################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccII AcyI AflII AflIII AgeI AlwNI Aor51HI ApaI AscI Asp700I AspI AvaII AviII AvrII BbeI BbrPI BcnI BfrI BglI BlnI BsaAI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CfoI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HpaI KasI Ksp632I KspI MluI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1945 GGTACC G^GTACC AccI 2718 GTCTAC GT^[AC][GT]AC AccIII 788 TCCGGA T^CCGGA AciI 3274 CCGC CCGC(-3/-1) AhdI 2710 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 2627 GTGCAC G^TGCAC AocI 856 CCTGAGG CC^T[ACGT]AGG ApaLI 2627 GTGCAC G^TGCAC Asp718I 1945 GGTACC G^GTACC AspEI 2710 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 633 TGGCCA TGG^CCA BamHI 3297 GGATCC G^GATCC BanI 1945 GGTACC G^G[CT][GA]CC BbvCI 3559 GCTGAGG CCTCAGC(-5/-2) BciVI 2680 GTATCC GTATCC(6/5) BclI 960 TGATCA T^GATCA BglII 2370 AGATCT A^GATCT BlpI 158 GCTTAGC GC^T[ACGT]AGC Bpu1102I 158 GCTTAGC GC^T[ACGT]AGC BsaBI 1144 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 788 TCCGGA [AT]^CCGG[AT] BseAI 788 TCCGGA T^CCGGA BsmAI 3315 GTCTC GTCTC(1/5) BspEI 788 TCCGGA T^CCGGA BspMI 2217 GCAGGT ACCTGC(4/8) BsrBI 3274 CCGCTC CCGCTC(-3/-3) BsrDI 520 CATTGC GCAATG(2/0) BstXI 2478 CCAGGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 856 CCTGAGG CC^T[ACGT]AGG CelII 158 GCTTAGC GC^T[ACGT]AGC Eam1105I 2710 GACAATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 856 CCTGAGG CC^T[ACGT]AGG FbaI 960 TGATCA T^GATCA HapII 789 CCGG C^CGG HgaI 2230 GACGC GACGC(5/10) HincII 1276 GTCAAC GT[CT]^[GA]AC HindII 1276 GTCAAC GT[CT]^[GA]AC HpaII 789 CCGG C^CGG KpnI 1945 GGTACC GGTAC^C MamI 1144 GATTTAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 633 TGGCCA TGG^CCA MroI 788 TCCGGA T^CCGGA MscI 633 TGGCCA TGG^CCA MspI 789 CCGG C^CGG PflMI 1516 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SphI 265 GCATGC GCATG^C StuI 1043 AGGCCT AGG^CCT SwaI 1145 ATTTAAAT ATTT^AAAT Van91I 1516 CCAATTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1116 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AclI 1733 AACGTT AA^CGTT 2117 AACGTT AseI 1336 ATTAAT AT^TAAT 1807 ATTAAT AsnI 1336 ATTAAT AT^TAAT 1807 ATTAAT BcgI 1194 CGAGGACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1315 CGAGCTGTGTGC BseRI 1531 GAGGAG GAGGAG(10/8) 2186 GAGGAG BspHI 1302 TCATGA T^CATGA 3678 TCATGA EaeI 633 TGGCCA [CT]^GGCC[GA] 2436 TGGCCG MfeI 26 CAATTG C^AATTG 247 CAATTG MslI 841 CATACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2503 CACAGAAATG MspA1I 1787 CAGCTG C[AC]G^C[GT]G 1900 CAGCTG MunI 26 CAATTG C^AATTG 247 CAATTG NspI 265 GCATGC [GA]CATG^[CT] 3728 GCATGT PshBI 1336 ATTAAT AT^TAAT 1807 ATTAAT Psp1406I 1733 AACGTT AA^CGTT 2117 AACGTT PstI 1751 CTGCAG CTGCA^G 2215 CTGCAG PvuII 1787 CAGCTG CAG^CTG 1900 CAGCTG RcaI 1302 TCATGA T^CATGA 3678 TCATGA Tsp45I 69 GTCAC ^GT[GC]AC 2731 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI AvaI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeIII HindIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII