(Circular) (Six-base) MAPSORT of: pFN21AE9282.seq Check: 341 from: 1 to: 8452 pFN21AE9282 8452 bp With 182 enzymes: SgfI * December 8, 2014 13:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4213 4213 Size: 8452 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6262 278 Size: 53 83 186 936 4726 2468 Fragments arranged by size: 4726 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4724 5795 1937 Size: 2787 1071 4594 Fragments arranged by size: 4594 2787 1071 Acc65I G'GTAC_C Cuts at: 3950 5773 3950 Size: 1823 6629 AclI AA'CG_TT Cuts at: 1563 3739 4123 6579 6952 1563 Size: 2176 384 2456 373 3063 Fragments arranged by size: 3063 2456 2176 384 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2265 5310 5442 6515 7899 8167 1632 Size: 633 3045 132 1073 1384 268 1917 Fragments arranged by size: 3045 1917 1384 1073 633 268 132 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8255 AflIII A'CryG_T Cuts at: 1203 7351 8328 1203 Size: 6148 977 1327 Fragments arranged by size: 6148 1327 977 AgeI A'CCGG_T Cuts at: 7283 7283 Size: 8452 AhdI GACnn_n'nnGTC Cuts at: 4720 7181 4720 Size: 2461 5991 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8452 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2921 1184 Size: 1737 6715 AlwNI CAG_nnn'CTG Cuts at: 6195 7767 6195 Size: 1572 6880 ApaLI G'TGCA_C Cuts at: 4632 6509 7665 4632 Size: 1877 1156 5419 Fragments arranged by size: 5419 1877 1156 ApoI r'AATT_y Cuts at: 1493 2092 2484 3931 4074 4204 4353 4458 4652 Size: 599 392 1447 143 130 149 105 194 Cuts at: 4652 4801 4867 5595 5761 5987 6139 8248 1493 Size: 149 66 728 166 226 152 2109 1697 Fragments arranged by size: 2109 1697 1447 728 599 392 226 194 166 152 149 149 143 130 105 66 AseI AT'TA_AT Cuts at: 160 1032 3342 3813 7006 160 Size: 872 2310 471 3193 1606 Fragments arranged by size: 3193 2310 1606 872 471 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8452 AvaI C'yCGr_G Cuts at: 1870 1943 3069 3210 3258 5072 5777 1870 Size: 73 1126 141 48 1814 705 4545 Fragments arranged by size: 4545 1814 1126 705 141 73 48 BamHI G'GATC_C Cuts at: 5302 5782 6149 5302 Size: 480 367 7605 Fragments arranged by size: 7605 480 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3950 5773 7228 618 Size: 325 217 627 2163 1823 1455 1842 Fragments arranged by size: 2163 1842 1823 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2793 4270 4528 5073 5771 1391 Size: 1402 1477 258 545 698 4072 Fragments arranged by size: 4072 1477 1402 698 545 258 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2001 4172 4424 928 Size: 971 102 2171 252 4956 Fragments arranged by size: 4956 2171 971 252 102 BbvCI CC'TCA_GC Cuts at: 5565 5565 Size: 8452 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3188 3222 3309 3343 6643 6677 1903 Size: 34 1251 34 87 34 3300 34 3678 Fragments arranged by size: 3678 3300 1251 87 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4696 6344 7554 4696 Size: 1648 1210 5594 Fragments arranged by size: 5594 1648 1210 BclI T'GATC_A Cuts at: 1548 2965 1548 Size: 1417 7035 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7063 136 Size: 107 122 71 631 605 5391 1525 Fragments arranged by size: 5391 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4375 8447 4375 Size: 4072 4380 BlpI GC'TnA_GC Cuts at: 2164 5869 2164 Size: 3705 4747 Bme1580I G_kGCm'C Cuts at: 1790 4636 6513 7669 1790 Size: 2846 1877 1156 2573 Fragments arranged by size: 2846 2573 1877 1156 BmgBI CAC'GTC Cuts at: 1318 2321 1318 Size: 1003 7449 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5681 6183 7141 Size: 416 53 83 399 24 4264 502 958 Cuts at: 7141 442 Size: 1753 Fragments arranged by size: 4264 1753 958 502 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8452 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3929 4175 4658 4879 7112 1239 Size: 2690 246 483 221 2233 2579 Fragments arranged by size: 2690 2579 2233 483 246 221 Bpu10I CC'TnA_GC Cuts at: 5067 5565 5067 Size: 498 7954 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6577 7442 7740 6577 Size: 865 298 7289 Fragments arranged by size: 7289 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7115 882 Size: 617 5616 2219 Fragments arranged by size: 5616 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6243 493 Size: 711 5039 2702 Fragments arranged by size: 5039 2702 711 BsaBI GATnn'nnATC Cuts at: 3153 8446 3153 Size: 5293 3159 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6259 6641 7276 275 Size: 53 83 186 936 4726 382 635 1451 Fragments arranged by size: 4726 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2793 6885 7283 7557 7704 8115 2793 Size: 4092 398 274 147 411 3130 Fragments arranged by size: 4092 3130 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 8227 Fragments arranged by size: 8227 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3551 4206 1186 Size: 2365 655 5432 Fragments arranged by size: 5432 2365 655 BseYI C'CCAG_C Cuts at: 7655 8151 7655 Size: 496 7956 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7879 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5902 6663 7691 1540 Size: 275 186 3901 761 1028 2301 Fragments arranged by size: 3901 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2793 3211 4039 4270 4528 4636 5073 6513 6598 Size: 418 828 231 258 108 437 1440 85 Cuts at: 6598 7669 2793 Size: 1071 3576 Fragments arranged by size: 3576 1440 1071 828 437 418 258 231 108 85 BsmI GAATG_Cn' Cuts at: 5963 6056 5963 Size: 93 8359 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2793 3211 4039 4270 4528 4636 5073 Size: 399 1003 418 828 231 258 108 437 Cuts at: 5073 5771 6513 6598 7669 1391 Size: 698 742 85 1071 2174 Fragments arranged by size: 2174 1071 1003 828 742 698 437 418 399 258 231 108 85 BspEI T'CCGG_A Cuts at: 2793 8115 2793 Size: 5322 3130 BspHI T'CATG_A Cuts at: 3307 5683 6341 8071 3307 Size: 2376 658 1730 3688 Fragments arranged by size: 3688 2376 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4213 5807 844 Size: 3369 1594 3489 Fragments arranged by size: 3489 3369 1594 BsrBI CCG'CTC Cuts at: 1394 1823 5281 6339 1394 Size: 429 3458 1058 3507 Fragments arranged by size: 3507 3458 1058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2524 6947 7121 65 Size: 1183 1276 4423 174 1396 Fragments arranged by size: 4423 1396 1276 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7096 7283 1700 Size: 110 5286 187 2869 Fragments arranged by size: 5286 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8452 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8452 BssSI C'ACGA_G Cuts at: 6512 7524 6512 Size: 1012 7440 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8452 BstBI TT'CG_AA Cuts at: 7256 7256 Size: 8452 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8452 BstXI CCAn_nnnn'nTGG Cuts at: 4490 4490 Size: 8452 BstYI r'GATC_y Cuts at: 1972 4375 4964 5302 5782 6149 6534 6551 8003 Size: 2403 589 338 480 367 385 17 1452 Cuts at: 8003 8089 8101 8447 1972 Size: 86 12 346 1977 Fragments arranged by size: 2403 1977 1452 589 480 385 367 346 338 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2862 2862 Size: 8452 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 7900 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7713 Fragments arranged by size: 7713 683 56 BtsI GCAGTG_nn' Cuts at: 3453 5968 6762 6782 3453 Size: 2515 794 20 5123 Fragments arranged by size: 5123 2515 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8452 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8417 DraI TTT'AAA Cuts at: 3153 4746 5707 5756 3153 Size: 1593 961 49 5849 Fragments arranged by size: 5849 1593 961 49 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7459 809 Size: 507 6143 1802 Fragments arranged by size: 6143 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2638 4441 5899 Size: 54 1425 50 275 18 808 1803 1458 Cuts at: 5899 6788 8417 8 Size: 889 1629 43 Fragments arranged by size: 1803 1629 1458 1425 889 808 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5899 1537 Size: 275 4087 4090 Fragments arranged by size: 4090 4087 275 EarI CTCTTCn'nnn_ Cuts at: 1353 6382 1353 Size: 5029 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7039 7413 7559 387 Size: 1064 138 5450 374 146 1280 Fragments arranged by size: 5450 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2791 4268 4526 5071 2791 Size: 1477 258 545 6172 Fragments arranged by size: 6172 1477 545 258 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2265 3929 4175 4658 4879 5310 5442 Size: 393 633 1664 246 483 221 431 132 Cuts at: 5442 6515 7112 7899 8167 1239 Size: 1073 597 787 268 1524 Fragments arranged by size: 1664 1524 1073 787 633 597 483 431 393 268 246 221 132 EcoO109I rG'GnC_Cy Cuts at: 3965 4473 4486 3965 Size: 508 13 7931 Fragments arranged by size: 7931 508 13 EcoRI G'AATT_C Cuts at: 2092 2484 4353 5595 5761 6139 2092 Size: 392 1869 1242 166 378 4405 Fragments arranged by size: 4405 1869 1242 392 378 166 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2068 3263 4995 2068 Size: 1195 1732 5525 Fragments arranged by size: 5525 1732 1195 FspI TGC'GCA Cuts at: 6958 6958 Size: 8452 HaeII r_GCGC'y Cuts at: 1678 7599 1678 Size: 5921 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3374 3406 4291 4323 4706 Size: 32 135 32 1802 32 885 32 383 Cuts at: 4706 4738 5060 5092 5330 5362 7093 7125 7167 Size: 32 322 32 238 32 1731 32 42 Cuts at: 7167 7199 1373 Size: 32 2626 Fragments arranged by size: 2626 1802 1731 885 383 322 238 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3283 5796 6042 669 Size: 988 281 1345 2513 246 3079 Fragments arranged by size: 3079 2513 1345 988 281 246 HindIII A'AGCT_T Cuts at: 748 2064 2161 5014 748 Size: 1316 97 2853 4186 Fragments arranged by size: 4186 2853 1316 97 HpaI GTT'AAC Cuts at: 6042 6042 Size: 8452 KpnI G_GTAC'C Cuts at: 3954 5777 3954 Size: 1823 6629 MfeI C'AATT_G Cuts at: 2031 2252 6051 2031 Size: 221 3799 4432 Fragments arranged by size: 4432 3799 221 MluI A'CGCG_T Cuts at: 8328 8328 Size: 8452 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 3956 7566 7750 1305 Size: 810 1841 3610 184 2007 Fragments arranged by size: 3610 2007 1841 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2640 10 Size: 54 1425 343 808 5822 Fragments arranged by size: 5822 1425 808 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2850 4512 6410 6769 6928 518 Size: 648 1684 1662 1898 359 159 2042 Fragments arranged by size: 2042 1898 1684 1662 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3794 3907 5868 6545 7693 7938 1606 Size: 2188 113 1961 677 1148 245 2120 Fragments arranged by size: 2188 2120 1961 1148 677 245 113 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7900 NdeI CA'TA_TG Cuts at: 387 387 Size: 8452 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8452 NotI GC'GGCC_GC Cuts at: 5899 5899 Size: 8452 NspI r_CATG'y Cuts at: 1132 1229 2274 5737 5810 7355 1132 Size: 97 1045 3463 73 1545 2229 Fragments arranged by size: 3463 2229 1545 1045 97 73 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8452 PciI A'CATG_T Cuts at: 7351 7351 Size: 8452 PflMI CCAn_nnn'nTGG Cuts at: 1108 3527 1108 Size: 2419 6033 PfoI T'CCnGG_A Cuts at: 3674 4833 4923 3674 Size: 1159 90 7203 Fragments arranged by size: 7203 1159 90 PmeI GTTT'AAAC Cuts at: 5756 5756 Size: 8452 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4113 4145 5265 5297 6521 6553 8034 8066 4113 Size: 32 1120 32 1224 32 1481 32 4499 Fragments arranged by size: 4499 1481 1224 1120 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8452 PsiI TTA'TAA Cuts at: 2061 3648 6022 2061 Size: 1587 2374 4491 Fragments arranged by size: 4491 2374 1587 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3225 3257 3225 Size: 32 8420 PstI C_TGCA'G Cuts at: 830 3760 4224 5804 830 Size: 2930 464 1580 3478 Fragments arranged by size: 3478 2930 1580 464 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8452 PvuII CAG'CTG Cuts at: 3794 3907 3794 Size: 113 8339 SacI G_AGCT'C Cuts at: 2793 4270 4528 5073 2793 Size: 1477 258 545 6172 Fragments arranged by size: 6172 1477 545 258 SalI G'TCGA_C Cuts at: 1936 5794 1936 Size: 3858 4594 SbfI CC_TGCA'GG Cuts at: 5804 5804 Size: 8452 ScaI AGT'ACT Cuts at: 6700 8412 6700 Size: 1712 6740 SfcI C'TryA_G Cuts at: 826 1045 3331 3756 4220 4379 4841 5800 6935 Size: 219 2286 425 464 159 462 959 1135 Cuts at: 6935 7616 7807 826 Size: 681 191 1471 Fragments arranged by size: 2286 1471 1135 959 681 464 462 425 219 191 159 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8452 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8452 SmaI CCC'GGG Cuts at: 1872 5779 1872 Size: 3907 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 6556 7457 7719 820 Size: 197 926 4613 901 262 1553 Fragments arranged by size: 4613 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6243 493 Size: 5750 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8452 SphI G_CATG'C Cuts at: 1132 2274 5810 1132 Size: 1142 3536 3774 Fragments arranged by size: 3774 3536 1142 SspI AAT'ATT Cuts at: 5 52 6376 5 Size: 47 6324 2081 Fragments arranged by size: 6324 2081 47 StuI AGG'CCT Cuts at: 1517 3050 1517 Size: 1533 6919 StyI C'CwwG_G Cuts at: 513 1065 2308 2989 3969 4069 4489 4644 5891 Size: 552 1243 681 980 100 420 155 1247 Cuts at: 5891 513 Size: 3074 Fragments arranged by size: 3074 1247 1243 980 681 552 420 155 100 SwaI ATTT'AAAT Cuts at: 3153 3153 Size: 8452 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 6479 6496 6649 6834 7279 325 Size: 1255 4899 17 153 185 445 1498 Fragments arranged by size: 4899 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2248 2542 4227 Size: 274 80 33 51 1443 271 294 1685 Cuts at: 4227 4749 6698 8410 96 Size: 522 1949 1712 138 Fragments arranged by size: 1949 1712 1685 1443 522 294 274 271 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3970 4002 4829 4861 8412 8444 3970 Size: 32 827 32 3551 32 3978 Fragments arranged by size: 3978 3551 827 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8452 XbaI T'CTAG_A Cuts at: 3121 5788 3121 Size: 2667 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8452 XmaI C'CCGG_G Cuts at: 1870 5777 1870 Size: 3907 4545 XmnI GAAnn'nnTTC Cuts at: 6581 6581 Size: 8452 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6260 276 Size: 53 83 186 936 4726 2468 Fragments arranged by size: 4726 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI