(Circular) (Six-base) MAPSORT of: pFN21AE6687.seq Check: 6686 from: 1 to: 5401 pFN21AE6687 5401 bp With 182 enzymes: SgfI * August 29, 2014 11:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3211 278 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2744 1937 Size: 807 4594 Acc65I G'GTAC_C Cuts at: 2722 2722 Size: 5401 AclI AA'CG_TT Cuts at: 1563 3528 3901 1563 Size: 1965 373 3063 Fragments arranged by size: 3063 1965 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2524 3464 4848 5116 1632 Size: 892 940 1384 268 1917 Fragments arranged by size: 1917 1384 940 892 268 AfeI AGC'GCT Cuts at: 2012 2549 2645 2012 Size: 537 96 4768 Fragments arranged by size: 4768 537 96 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5204 AflIII A'CryG_T Cuts at: 1203 2481 4300 5277 1203 Size: 1278 1819 977 1327 Fragments arranged by size: 1819 1327 1278 977 AgeI A'CCGG_T Cuts at: 4232 4232 Size: 5401 AhdI GACnn_n'nnGTC Cuts at: 4130 4130 Size: 5401 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5401 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2441 1184 Size: 1257 4144 AlwNI CAG_nnn'CTG Cuts at: 2326 3144 4716 2326 Size: 818 1572 3011 Fragments arranged by size: 3011 1572 818 ApaLI G'TGCA_C Cuts at: 2597 3458 4614 2597 Size: 861 1156 3384 Fragments arranged by size: 3384 1156 861 ApoI r'AATT_y Cuts at: 1493 2710 2936 3088 5197 1493 Size: 1217 226 152 2109 1697 Fragments arranged by size: 2109 1697 1217 226 152 AseI AT'TA_AT Cuts at: 160 1032 3955 160 Size: 872 2923 1606 Fragments arranged by size: 2923 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 AvaI C'yCGr_G Cuts at: 1870 1943 2213 2255 2564 2726 1870 Size: 73 270 42 309 162 4545 Fragments arranged by size: 4545 309 270 162 73 42 AvrII C'CTAG_G Cuts at: 2023 2023 Size: 5401 BamHI G'GATC_C Cuts at: 2731 3098 2731 Size: 367 5034 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2722 4177 618 Size: 325 217 627 323 612 1455 1842 Fragments arranged by size: 1842 1455 627 612 325 323 217 BanII G_rGCy'C Cuts at: 1391 2720 1391 Size: 1329 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3592 3626 1903 Size: 34 1655 34 3678 Fragments arranged by size: 3678 1655 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3293 4503 3293 Size: 1210 4191 BclI T'GATC_A Cuts at: 1548 1548 Size: 5401 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4012 136 Size: 107 122 71 631 605 2340 1525 Fragments arranged by size: 2340 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5396 5396 Size: 5401 BlpI GC'TnA_GC Cuts at: 2818 2818 Size: 5401 Bme1580I G_kGCm'C Cuts at: 1790 2565 2601 3462 4618 1790 Size: 775 36 861 1156 2573 Fragments arranged by size: 2573 1156 861 775 36 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5401 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3132 4090 442 Size: 416 53 83 399 24 1715 958 1753 Fragments arranged by size: 1753 1715 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4061 1239 Size: 2822 2579 Bpu10I CC'TnA_GC Cuts at: 2414 2414 Size: 5401 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3526 4391 4689 3526 Size: 865 298 4238 Fragments arranged by size: 4238 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4064 882 Size: 617 2565 2219 Fragments arranged by size: 2565 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2521 3192 493 Size: 711 1317 671 2702 Fragments arranged by size: 2702 1317 711 671 BsaBI GATnn'nnATC Cuts at: 2660 5395 2660 Size: 2735 2666 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3208 3590 4225 275 Size: 53 83 186 936 1675 382 635 1451 Fragments arranged by size: 1675 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3834 4232 4506 4653 5064 3834 Size: 398 274 147 411 4171 Fragments arranged by size: 4171 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5176 Fragments arranged by size: 5176 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5401 BseYI C'CCAG_C Cuts at: 2018 2114 2288 4604 5100 2018 Size: 96 174 2316 496 2319 Fragments arranged by size: 2319 2316 496 174 96 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2304 1163 Size: 573 568 4260 Fragments arranged by size: 4260 573 568 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 2339 2851 3612 4640 1540 Size: 275 186 78 260 512 761 1028 2301 Fragments arranged by size: 2301 1028 761 512 275 260 186 78 BsiHKAI G_wGCw'C Cuts at: 2558 2601 2696 3462 3547 4618 2558 Size: 43 95 766 85 1071 3341 Fragments arranged by size: 3341 1071 766 95 85 43 BsmI GAATG_Cn' Cuts at: 2912 3005 2912 Size: 93 5308 BsmBI CGTCTCn'nnnn_ Cuts at: 2186 2186 Size: 5401 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2558 2565 2601 2696 2720 3462 3547 Size: 399 768 7 36 95 24 742 85 Cuts at: 3547 4618 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 768 742 399 95 85 36 24 7 BspEI T'CCGG_A Cuts at: 5064 5064 Size: 5401 BspHI T'CATG_A Cuts at: 3290 5020 3290 Size: 1730 3671 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2133 2756 844 Size: 1289 623 3489 Fragments arranged by size: 3489 1289 623 BsrBI CCG'CTC Cuts at: 1394 1823 3288 1394 Size: 429 1465 3507 Fragments arranged by size: 3507 1465 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3896 4070 65 Size: 1183 2648 174 1396 Fragments arranged by size: 2648 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2074 2078 2262 4045 4232 1700 Size: 110 264 4 184 1783 187 2869 Fragments arranged by size: 2869 1783 264 187 184 110 4 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5401 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5401 BssSI C'ACGA_G Cuts at: 2551 2695 3461 4473 2551 Size: 144 766 1012 3479 Fragments arranged by size: 3479 1012 766 144 BstAPI GCAn_nnn'nTGC Cuts at: 794 2577 794 Size: 1783 3618 BstBI TT'CG_AA Cuts at: 4205 4205 Size: 5401 BstEII G'GTnAC_C Cuts at: 1184 2029 1184 Size: 845 4556 BstYI r'GATC_y Cuts at: 1972 2182 2731 3098 3483 3500 4952 5038 5050 Size: 210 549 367 385 17 1452 86 12 Cuts at: 5050 5396 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 549 385 367 346 210 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3910 Fragments arranged by size: 3910 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4662 Fragments arranged by size: 4662 683 56 BtsI GCAGTG_nn' Cuts at: 2917 3711 3731 2917 Size: 794 20 4587 Fragments arranged by size: 4587 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5401 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5366 DraI TTT'AAA Cuts at: 2705 2705 Size: 5401 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4408 809 Size: 507 3092 1802 Fragments arranged by size: 3092 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2083 2241 Size: 54 1425 50 275 18 246 7 158 Cuts at: 2241 2336 2510 2848 3737 5366 8 Size: 95 174 338 889 1629 43 Fragments arranged by size: 1629 1425 889 338 275 246 174 158 95 54 50 43 18 7 EagI C'GGCC_G Cuts at: 1537 1812 2076 2336 2848 1537 Size: 275 264 260 512 4090 Fragments arranged by size: 4090 512 275 264 260 EarI CTCTTCn'nnn_ Cuts at: 1353 3331 1353 Size: 1978 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2093 2324 3988 4362 4508 387 Size: 1064 138 504 231 1664 374 146 1280 Fragments arranged by size: 1664 1280 1064 504 374 231 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2524 3464 4061 4848 5116 1239 Size: 393 892 940 597 787 268 1524 Fragments arranged by size: 1524 940 892 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2302 2302 Size: 5401 EcoRI G'AATT_C Cuts at: 2710 3088 2710 Size: 378 5023 FspI TGC'GCA Cuts at: 3907 3907 Size: 5401 HaeII r_GCGC'y Cuts at: 1678 2014 2413 2551 2623 2647 4548 1678 Size: 336 399 138 72 24 1901 2531 Fragments arranged by size: 2531 1901 399 336 138 72 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4042 4074 4116 4148 1373 Size: 32 135 32 2470 32 42 32 2626 Fragments arranged by size: 2626 2470 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2745 2991 669 Size: 988 281 807 246 3079 Fragments arranged by size: 3079 988 807 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5401 HpaI GTT'AAC Cuts at: 2991 2991 Size: 5401 KpnI G_GTAC'C Cuts at: 2726 2726 Size: 5401 MfeI C'AATT_G Cuts at: 3000 3000 Size: 5401 MluI A'CGCG_T Cuts at: 2481 5277 2481 Size: 2796 2605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4515 4699 1305 Size: 3210 184 2007 Fragments arranged by size: 3210 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2243 10 Size: 54 1425 343 411 3168 Fragments arranged by size: 3168 1425 411 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3359 3718 3877 518 Size: 648 2193 359 159 2042 Fragments arranged by size: 2193 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2288 2323 2335 2535 2629 2817 3494 4642 Size: 682 35 12 200 94 188 677 1148 Cuts at: 4642 4887 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 682 677 245 200 188 94 35 12 NaeI GCC'GGC Cuts at: 2080 2080 Size: 5401 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3910 Fragments arranged by size: 3910 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5401 NgoMIV G'CCGG_C Cuts at: 2078 2078 Size: 5401 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5401 NotI GC'GGCC_GC Cuts at: 2848 2848 Size: 5401 NspI r_CATG'y Cuts at: 1132 1229 2759 4304 1132 Size: 97 1530 1545 2229 Fragments arranged by size: 2229 1545 1530 97 PasI CC'CwG_GG Cuts at: 1348 2395 1348 Size: 1047 4354 PciI A'CATG_T Cuts at: 4300 4300 Size: 5401 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5401 PmeI GTTT'AAAC Cuts at: 2705 2705 Size: 5401 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3470 3502 4983 5015 3470 Size: 32 1481 32 3856 Fragments arranged by size: 3856 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2302 2302 Size: 5401 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5401 PsiI TTA'TAA Cuts at: 2971 2971 Size: 5401 PstI C_TGCA'G Cuts at: 830 2753 830 Size: 1923 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5401 PvuII CAG'CTG Cuts at: 2288 2323 2535 2288 Size: 35 212 5154 Fragments arranged by size: 5154 212 35 SalI G'TCGA_C Cuts at: 1936 2743 1936 Size: 807 4594 SbfI CC_TGCA'GG Cuts at: 2753 2753 Size: 5401 ScaI AGT'ACT Cuts at: 3649 5361 3649 Size: 1712 3689 SfcI C'TryA_G Cuts at: 826 1045 2749 3884 4565 4756 826 Size: 219 1704 1135 681 191 1471 Fragments arranged by size: 1704 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5401 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5401 SmaI CCC'GGG Cuts at: 1872 2728 1872 Size: 856 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3505 4406 4668 820 Size: 197 926 1562 901 262 1553 Fragments arranged by size: 1562 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3192 493 Size: 2699 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5401 SphI G_CATG'C Cuts at: 1132 2759 1132 Size: 1627 3774 SspI AAT'ATT Cuts at: 5 52 3325 5 Size: 47 3273 2081 Fragments arranged by size: 3273 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5401 StyI C'CwwG_G Cuts at: 513 1065 2004 2023 2172 2840 513 Size: 552 939 19 149 668 3074 Fragments arranged by size: 3074 939 668 552 149 19 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2128 2166 3428 3445 3598 3783 4228 Size: 1255 548 38 1262 17 153 185 445 Cuts at: 4228 325 Size: 1498 Fragments arranged by size: 1498 1262 1255 548 445 185 153 38 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3647 5359 96 Size: 274 80 33 51 1443 1670 1712 138 Fragments arranged by size: 1712 1670 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5361 5393 5361 Size: 32 5369 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5401 XbaI T'CTAG_A Cuts at: 2737 2737 Size: 5401 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5401 XmaI C'CCGG_G Cuts at: 1870 2726 1870 Size: 856 4545 XmnI GAAnn'nnTTC Cuts at: 3530 3530 Size: 5401 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3209 276 Size: 53 83 186 936 1675 2468 Fragments arranged by size: 2468 1675 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI