################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII AccI AclI AcsI AcyI AflII AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfrI BlnI BpuAI BsaAI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI MaeII MfeI MluI MseI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI SacI SalI SapI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 853 GGTACC G^GTACC AccIII 776 TCCGGA T^CCGGA AfaI 854 GTAC GT^AC Alw44I 717 GTGCAC G^TGCAC AlwI 488 GGATC GGATC(4/5) ApaLI 717 GTGCAC G^TGCAC Asp700I 570 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 853 GGTACC G^GTACC AspHI 717 GTGCAC G[AT]GC[AT]^C BalI 5 TGGCCA TGG^CCA BcgI 823 CGAGAGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 692 CTAG C^TAG BglII 168 AGATCT A^GATCT BsaBI 163 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 776 TCCGGA [AT]^CCGG[AT] BseAI 776 TCCGGA T^CCGGA BsiHKAI 717 GTGCAC G[AT]GC[AT]^C BspEI 776 TCCGGA T^CCGGA BspHI 108 TCATGA T^CATGA BsrFI 721 ACCGGC [GA]^CCGG[CT] BstAPI 702 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 721 ACCGGC [GA]^CCGG[CT] CpoI 778 CGGACCG CG^G[AT]CCG EarI 385 GAAGAG CTCTTC(1/4) EcoNI 51 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 483 CCATGG C^C[AT][AT]GG HgaI 105 GCGTC GACGC(5/10) KpnI 853 GGTACC GGTAC^C Ksp632I 385 GAAGAG CTCTTC(1/4) KspI 139 CCGCGG CCGC^GG MaeI 692 CTAG C^TAG MaeIII 39 GTCAC ^GT[ACGT]AC MamI 163 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 5 TGGCCA TGG^CCA MroI 776 TCCGGA T^CCGGA MscI 5 TGGCCA TGG^CCA NcoI 483 CCATGG C^CATGG PflMI 405 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 452 GGGACCC [GA]G^G[AT]CC[CT] PvuII 706 CAGCTG CAG^CTG RcaI 108 TCATGA T^CATGA RsaI 854 GTAC GT^AC RsrII 778 CGGACCG CG^G[AT]CCG SacII 139 CCGCGG CCGC^GG SbfI 612 CCTGCAGG CCTGCA^GG Sse8387I 612 CCTGCAGG CCTGCA^GG StyI 483 CCATGG C^C[AT][AT]GG TaqI 286 TCGA T^CGA Tsp45I 39 GTCAC ^GT[GC]AC TthHB8I 286 TCGA T^CGA Van91I 405 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 570 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccII 140 CGCG CG^CG 382 CGCG AvaI 78 CCCGGG C^[CT]CG[GA]G 374 CCCGGG BanI 73 GGTGCC G^G[CT][GA]CC 853 GGTACC BglI 227 GCCCAGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 611 GCCTGCAGGGC BlpI 239 GCTCAGC GC^T[ACGT]AGC 321 GCTGAGC Bpu1102I 239 GCTCAGC GC^T[ACGT]AGC 321 GCTGAGC BsoBI 78 CCCGGG C^[CT]CG[GA]G 374 CCCGGG BstUI 140 CGCG CG^CG 382 CGCG BstYI 168 AGATCT [GA]^GATC[CT] 488 GGATCT CelII 239 GCTCAGC GC^T[ACGT]AGC 321 GCTGAGC CfoI 291 GCGC GCG^C 550 GCGC DpnII 169 GATC ^GATC 489 GATC DraII 367 AGGGCCT [GA]G^G[ACGT]CC[CT] 452 GGGACCC EcoO109I 367 AGGGCCT [GA]G^G[ACGT]CC[CT] 452 GGGACCC FauI 345 GCGGG CCCGC(4/6) 540 GCGGG HhaI 291 GCGC GCG^C 550 GCGC HinP1I 291 GCGC G^CGC 550 GCGC HphI 556 GGTGA GGTGA(8/7) 40 TCACC MboI 169 GATC ^GATC 489 GATC MflI 168 AGATCT [GA]^GATC[CT] 488 GGATCT MvnI 140 CGCG CG^CG 382 CGCG NdeII 169 GATC ^GATC 489 GATC NlaIII 109 CATG CATG^ 484 CATG Sau3AI 169 GATC ^GATC 489 GATC SmaI 78 CCCGGG CCC^GGG 374 CCCGGG XhoII 168 AGATCT [GA]^GATC[CT] 488 GGATCT XmaCI 78 CCCGGG C^CCGGG 374 CCCGGG XmaI 78 CCCGGG C^CCGGG 374 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AluI AlwNI AvaII BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DsaI EaeI EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MboII MnlI MspA1I MspI MvaI MwoI NciI NlaIV PleI PstI Sau96I ScrFI SfcI TseI TspRI