Restriction map for pFN21AE5318
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AcyI        
AflII       AflIII      AgeI        AhdI        AocI        
Aor51HI     ApaI        ApoI        AscI        AseI        
AsnI        AspEI       AspI        AviII       AvrII       
BamHI       BbeI        BbrPI       BbsI        BbvCI       
BciVI       BclI        BfrI        BlnI        BpuAI       
BsaAI       BsaHI       BsaI        BsgI        BsiEI       
BsiWI       BsmBI       BsmI        Bsp1407I    BspDI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      ClaI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoO65I     
EcoRI       EcoRV       EcoT22I     FbaI        FseI        
FspI        HaeII       Hin1I       HincII      HindII      
HindIII     HpaI        KasI        MaeII       MfeI        
MluI        MseI        MslI        MunI        NaeI        
NarI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
SacI        SalI        SapI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmlI        SnaBI       SpeI        SphI        SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        853  GGTACC               G^GTACC
AccIII        776  TCCGGA               T^CCGGA
AfaI          854  GTAC                 GT^AC
Alw44I        717  GTGCAC               G^TGCAC
AlwI          488  GGATC                GGATC(4/5)
ApaLI         717  GTGCAC               G^TGCAC
Asp700I       570  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       853  GGTACC               G^GTACC
AspHI         717  GTGCAC               G[AT]GC[AT]^C
BalI            5  TGGCCA               TGG^CCA
BcgI          823  CGAGAGGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI          692  CTAG                 C^TAG
BglII         168  AGATCT               A^GATCT
BsaBI         163  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI         776  TCCGGA               [AT]^CCGG[AT]
BseAI         776  TCCGGA               T^CCGGA
BsiHKAI       717  GTGCAC               G[AT]GC[AT]^C
BspEI         776  TCCGGA               T^CCGGA
BspHI         108  TCATGA               T^CATGA
BsrFI         721  ACCGGC               [GA]^CCGG[CT]
BstAPI        702  GCAGCAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I        721  ACCGGC               [GA]^CCGG[CT]
CpoI          778  CGGACCG              CG^G[AT]CCG
EarI          385  GAAGAG               CTCTTC(1/4)
EcoNI          51  CCTGGAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I       483  CCATGG               C^C[AT][AT]GG
HgaI          105  GCGTC                GACGC(5/10)
KpnI          853  GGTACC               GGTAC^C
Ksp632I       385  GAAGAG               CTCTTC(1/4)
KspI          139  CCGCGG               CCGC^GG
MaeI          692  CTAG                 C^TAG
MaeIII         39  GTCAC                ^GT[ACGT]AC
MamI          163  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI           5  TGGCCA               TGG^CCA
MroI          776  TCCGGA               T^CCGGA
MscI            5  TGGCCA               TGG^CCA
NcoI          483  CCATGG               C^CATGG
PflMI         405  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         452  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         706  CAGCTG               CAG^CTG
RcaI          108  TCATGA               T^CATGA
RsaI          854  GTAC                 GT^AC
RsrII         778  CGGACCG              CG^G[AT]CCG
SacII         139  CCGCGG               CCGC^GG
SbfI          612  CCTGCAGG             CCTGCA^GG
Sse8387I      612  CCTGCAGG             CCTGCA^GG
StyI          483  CCATGG               C^C[AT][AT]GG
TaqI          286  TCGA                 T^CGA
Tsp45I         39  GTCAC                ^GT[GC]AC
TthHB8I       286  TCGA                 T^CGA
Van91I        405  CCAGCCCGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI          570  GAAGGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccII         140  CGCG                 CG^CG
              382  CGCG                
AvaI           78  CCCGGG               C^[CT]CG[GA]G
              374  CCCGGG              
BanI           73  GGTGCC               G^G[CT][GA]CC
              853  GGTACC              
BglI          227  GCCCAGGGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
              611  GCCTGCAGGGC         
BlpI          239  GCTCAGC              GC^T[ACGT]AGC
              321  GCTGAGC             
Bpu1102I      239  GCTCAGC              GC^T[ACGT]AGC
              321  GCTGAGC             
BsoBI          78  CCCGGG               C^[CT]CG[GA]G
              374  CCCGGG              
BstUI         140  CGCG                 CG^CG
              382  CGCG                
BstYI         168  AGATCT               [GA]^GATC[CT]
              488  GGATCT              
CelII         239  GCTCAGC              GC^T[ACGT]AGC
              321  GCTGAGC             
CfoI          291  GCGC                 GCG^C
              550  GCGC                
DpnII         169  GATC                 ^GATC
              489  GATC                
DraII         367  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              452  GGGACCC             
EcoO109I      367  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              452  GGGACCC             
FauI          345  GCGGG                CCCGC(4/6)
              540  GCGGG               
HhaI          291  GCGC                 GCG^C
              550  GCGC                
HinP1I        291  GCGC                 G^CGC
              550  GCGC                
HphI          556  GGTGA                GGTGA(8/7)
               40  TCACC               
MboI          169  GATC                 ^GATC
              489  GATC                
MflI          168  AGATCT               [GA]^GATC[CT]
              488  GGATCT              
MvnI          140  CGCG                 CG^CG
              382  CGCG                
NdeII         169  GATC                 ^GATC
              489  GATC                
NlaIII        109  CATG                 CATG^
              484  CATG                
Sau3AI        169  GATC                 ^GATC
              489  GATC                
SmaI           78  CCCGGG               CCC^GGG
              374  CCCGGG              
XhoII         168  AGATCT               [GA]^GATC[CT]
              488  GGATCT              
XmaCI          78  CCCGGG               C^CCGGG
              374  CCCGGG              
XmaI           78  CCCGGG               C^CCGGG
              374  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   48 enzymes) #
#########################################

AciI        AluI        AlwNI       AvaII       BanII       
BbvI        BcnI        BmyI        BpmI        BsaJI       
BseRI       BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BsrI        BssKI       BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DsaI        EaeI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       ItaI        MboII       MnlI        
MspA1I      MspI        MvaI        MwoI        NciI        
NlaIV       PleI        PstI        Sau96I      ScrFI       
SfcI        TseI        TspRI       
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