(Circular) (Six-base) MAPSORT of: pFN21AE5318.seq Check: 979 from: 1 to: 5563 pFN21AE5318 5563 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3373 278 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2906 1937 Size: 969 4594 Acc65I G'GTAC_C Cuts at: 2858 2884 2858 Size: 26 5537 AclI AA'CG_TT Cuts at: 1563 3690 4063 1563 Size: 2127 373 3063 Fragments arranged by size: 3063 2127 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3626 5010 5278 1632 Size: 1994 1384 268 1917 Fragments arranged by size: 1994 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5366 AflIII A'CryG_T Cuts at: 1203 4462 5439 1203 Size: 3259 977 1327 Fragments arranged by size: 3259 1327 977 AgeI A'CCGG_T Cuts at: 4394 4394 Size: 5563 AhdI GACnn_n'nnGTC Cuts at: 4292 4292 Size: 5563 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5563 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5563 AlwNI CAG_nnn'CTG Cuts at: 2443 2641 2713 3306 4878 2443 Size: 198 72 593 1572 3128 Fragments arranged by size: 3128 1572 593 198 72 ApaLI G'TGCA_C Cuts at: 2722 3620 4776 2722 Size: 898 1156 3509 Fragments arranged by size: 3509 1156 898 ApoI r'AATT_y Cuts at: 1493 2872 3098 3250 5359 1493 Size: 1379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 226 152 AseI AT'TA_AT Cuts at: 160 1032 4117 160 Size: 872 3085 1606 Fragments arranged by size: 3085 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 AvaI C'yCGr_G Cuts at: 1870 1943 2083 2379 2888 1870 Size: 73 140 296 509 4545 Fragments arranged by size: 4545 509 296 140 73 BamHI G'GATC_C Cuts at: 2893 3260 2893 Size: 367 5196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2858 2884 4339 618 Size: 325 217 627 291 780 26 1455 1842 Fragments arranged by size: 1842 1455 780 627 325 291 217 26 BanII G_rGCy'C Cuts at: 1391 2093 2234 2472 2882 1391 Size: 702 141 238 410 4072 Fragments arranged by size: 4072 702 410 238 141 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4592 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2817 2851 3754 3788 1903 Size: 34 880 34 903 34 3678 Fragments arranged by size: 3678 903 880 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3455 4665 3455 Size: 1210 4353 BclI T'GATC_A Cuts at: 1548 1548 Size: 5563 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2238 2622 Size: 107 122 71 631 605 337 229 384 Cuts at: 2622 4174 136 Size: 1552 1525 Fragments arranged by size: 1552 1525 631 605 384 337 229 122 107 71 BglII A'GATC_T Cuts at: 2173 5558 2173 Size: 3385 2178 BlpI GC'TnA_GC Cuts at: 2245 2327 2980 2245 Size: 82 653 4828 Fragments arranged by size: 4828 653 82 Bme1580I G_kGCm'C Cuts at: 1790 2083 2487 2726 3624 4780 1790 Size: 293 404 239 898 1156 2573 Fragments arranged by size: 2573 1156 898 404 293 239 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5563 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2431 3294 4252 Size: 416 53 83 399 24 1014 863 958 Cuts at: 4252 442 Size: 1753 Fragments arranged by size: 1753 1014 958 863 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5563 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2176 2176 Size: 5563 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2342 2678 2684 4223 1239 Size: 839 264 336 6 1539 2579 Fragments arranged by size: 2579 1539 839 336 264 6 Bpu10I CC'TnA_GC Cuts at: 2106 2106 Size: 5563 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3688 4553 4851 3688 Size: 865 298 4400 Fragments arranged by size: 4400 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4226 882 Size: 617 2727 2219 Fragments arranged by size: 2727 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3354 493 Size: 711 2150 2702 Fragments arranged by size: 2702 2150 711 BsaBI GATnn'nnATC Cuts at: 2172 5557 2172 Size: 3385 2178 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3370 3752 4387 275 Size: 53 83 186 936 1837 382 635 1451 Fragments arranged by size: 1837 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2781 3996 4394 4668 4815 5226 2781 Size: 1215 398 274 147 411 3118 Fragments arranged by size: 3118 1215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5338 Fragments arranged by size: 5338 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2075 2480 2702 2705 2768 2864 1186 Size: 889 405 222 3 63 96 3885 Fragments arranged by size: 3885 889 405 222 96 63 3 BseYI C'CCAG_C Cuts at: 2180 2331 4766 5262 2180 Size: 151 2435 496 2481 Fragments arranged by size: 2481 2435 496 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4990 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3013 3774 4802 1540 Size: 275 186 1012 761 1028 2301 Fragments arranged by size: 2301 1028 1012 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2726 3624 3709 4780 2726 Size: 898 85 1071 3509 Fragments arranged by size: 3509 1071 898 85 BsmI GAATG_Cn' Cuts at: 3074 3167 3074 Size: 93 5470 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2093 2234 2472 2487 2726 2882 Size: 399 293 10 141 238 15 239 156 Cuts at: 2882 3624 3709 4780 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 293 239 238 156 141 85 15 10 BspEI T'CCGG_A Cuts at: 2781 5226 2781 Size: 2445 3118 BspHI T'CATG_A Cuts at: 2113 3452 5182 2113 Size: 1339 1730 2494 Fragments arranged by size: 2494 1730 1339 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2918 844 Size: 2074 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3450 1394 Size: 429 1627 3507 Fragments arranged by size: 3507 1627 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4058 4232 65 Size: 1183 2810 174 1396 Fragments arranged by size: 2810 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2726 4207 4394 1700 Size: 110 916 1481 187 2869 Fragments arranged by size: 2869 1481 916 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5563 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5563 BssSI C'ACGA_G Cuts at: 3623 4635 3623 Size: 1012 4551 BstAPI GCAn_nnn'nTGC Cuts at: 794 2713 794 Size: 1919 3644 BstBI TT'CG_AA Cuts at: 4367 4367 Size: 5563 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5563 BstYI r'GATC_y Cuts at: 1972 2173 2493 2893 3260 3645 3662 5114 5200 Size: 201 320 400 367 385 17 1452 86 Cuts at: 5200 5212 5558 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 400 385 367 346 320 201 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2144 2415 2488 513 Size: 552 1079 271 73 3588 Fragments arranged by size: 3588 1079 552 271 73 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4824 Fragments arranged by size: 4824 683 56 BtsI GCAGTG_nn' Cuts at: 3079 3873 3893 3079 Size: 794 20 4749 Fragments arranged by size: 4749 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5563 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5528 DraI TTT'AAA Cuts at: 2867 2867 Size: 5563 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4570 809 Size: 507 3254 1802 Fragments arranged by size: 3254 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 2285 2728 Size: 54 1425 50 275 18 180 275 443 Cuts at: 2728 3010 3899 5528 8 Size: 282 889 1629 43 Fragments arranged by size: 1629 1425 889 443 282 275 275 180 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3010 1537 Size: 275 1198 4090 Fragments arranged by size: 4090 1198 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 3493 1353 Size: 1032 1108 3423 Fragments arranged by size: 3423 1108 1032 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2699 4150 4524 4670 387 Size: 1064 138 1110 1451 374 146 1280 Fragments arranged by size: 1451 1280 1110 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2342 2678 2684 3626 4223 5010 Size: 393 446 264 336 6 942 597 787 Cuts at: 5010 5278 1239 Size: 268 1524 Fragments arranged by size: 1524 942 787 597 446 393 336 268 264 6 EcoNI CCTnn'n_nnAGG Cuts at: 2060 2060 Size: 5563 EcoO109I rG'GnC_Cy Cuts at: 2373 2458 2373 Size: 85 5478 EcoRI G'AATT_C Cuts at: 2872 3250 2872 Size: 378 5185 FspI TGC'GCA Cuts at: 4069 4069 Size: 5563 HaeII r_GCGC'y Cuts at: 1678 4710 1678 Size: 3032 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 1999 2031 4204 4236 4278 Size: 32 135 32 427 32 2173 32 42 Cuts at: 4278 4310 1373 Size: 32 2626 Fragments arranged by size: 2626 2173 427 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2907 3153 669 Size: 988 281 969 246 3079 Fragments arranged by size: 3079 988 969 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5563 HpaI GTT'AAC Cuts at: 3153 3153 Size: 5563 KpnI G_GTAC'C Cuts at: 2862 2888 2862 Size: 26 5537 MfeI C'AATT_G Cuts at: 3162 3162 Size: 5563 MluI A'CGCG_T Cuts at: 5439 5439 Size: 5563 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4677 4861 1305 Size: 3372 184 2007 Fragments arranged by size: 3372 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2012 10 Size: 54 1425 343 180 3561 Fragments arranged by size: 3561 1425 343 180 54 MslI CAynn'nnrTG Cuts at: 518 1166 3521 3880 4039 518 Size: 648 2355 359 159 2042 Fragments arranged by size: 2355 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2146 2249 2350 2572 2713 2797 2841 2979 Size: 540 103 101 222 141 84 44 138 Cuts at: 2979 3656 4804 5049 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 540 245 222 141 138 103 101 84 44 NcoI C'CATG_G Cuts at: 513 1065 2488 513 Size: 552 1423 3588 Fragments arranged by size: 3588 1423 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5563 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5563 NotI GC'GGCC_GC Cuts at: 3010 3010 Size: 5563 NspI r_CATG'y Cuts at: 1132 1229 2921 4466 1132 Size: 97 1692 1545 2229 Fragments arranged by size: 2229 1692 1545 97 PasI CC'CwG_GG Cuts at: 1348 2234 1348 Size: 886 4677 PciI A'CATG_T Cuts at: 4462 4462 Size: 5563 PflMI CCAn_nnn'nTGG Cuts at: 1108 2416 1108 Size: 1308 4255 PmeI GTTT'AAAC Cuts at: 2867 2867 Size: 5563 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3632 3664 5145 5177 3632 Size: 32 1481 32 4018 Fragments arranged by size: 4018 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2458 2458 Size: 5563 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5563 PsiI TTA'TAA Cuts at: 3133 3133 Size: 5563 PstI C_TGCA'G Cuts at: 830 2622 2709 2718 2915 830 Size: 1792 87 9 197 3478 Fragments arranged by size: 3478 1792 197 87 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5563 PvuII CAG'CTG Cuts at: 2713 2713 Size: 5563 RsrII CG'GwC_CG Cuts at: 2784 2784 Size: 5563 SacII CC_GC'GG Cuts at: 2147 2147 Size: 5563 SalI G'TCGA_C Cuts at: 1936 2905 1936 Size: 969 4594 SanDI GG'GwC_CC Cuts at: 2458 2458 Size: 5563 SbfI CC_TGCA'GG Cuts at: 2622 2915 2622 Size: 293 5270 ScaI AGT'ACT Cuts at: 3811 5523 3811 Size: 1712 3851 SfcI C'TryA_G Cuts at: 826 1045 2618 2705 2714 2911 4046 4727 4918 Size: 219 1573 87 9 197 1135 681 191 Cuts at: 4918 826 Size: 1471 Fragments arranged by size: 1573 1471 1135 681 219 197 191 87 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5563 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5563 SmaI CCC'GGG Cuts at: 1872 2085 2381 2890 1872 Size: 213 296 509 4545 Fragments arranged by size: 4545 509 296 213 SmlI C'TyrA_G Cuts at: 820 1017 1943 3667 4568 4830 820 Size: 197 926 1724 901 262 1553 Fragments arranged by size: 1724 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3354 493 Size: 2861 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5563 SphI G_CATG'C Cuts at: 1132 2921 1132 Size: 1789 3774 SspI AAT'ATT Cuts at: 5 52 3487 5 Size: 47 3435 2081 Fragments arranged by size: 3435 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5563 StyI C'CwwG_G Cuts at: 513 1065 2488 3002 513 Size: 552 1423 514 3074 Fragments arranged by size: 3074 1423 552 514 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 2517 2801 3590 3607 3760 3945 Size: 1255 870 67 284 789 17 153 185 Cuts at: 3945 4390 325 Size: 445 1498 Fragments arranged by size: 1498 1255 870 789 445 284 185 153 67 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3809 5521 96 Size: 274 80 33 51 1443 1832 1712 138 Fragments arranged by size: 1832 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2492 2524 5523 5555 2492 Size: 32 2999 32 2500 Fragments arranged by size: 2999 2500 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5563 XbaI T'CTAG_A Cuts at: 2899 2899 Size: 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5563 XmaI C'CCGG_G Cuts at: 1870 2083 2379 2888 1870 Size: 213 296 509 4545 Fragments arranged by size: 4545 509 296 213 XmnI GAAnn'nnTTC Cuts at: 2579 3692 2579 Size: 1113 4450 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3371 276 Size: 53 83 186 936 1837 2468 Fragments arranged by size: 2468 1837 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI