Restriction map for pFN21AE5318
 (Circular) (Six-base) MAPSORT of: pFN21AE5318.seq  Check: 979  from: 1  to: 5563



pFN21AE5318  5563 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:46  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3373     278 
   Size:         53      83     186     936    1837    2468

  Fragments arranged by size: 

                2468    1837     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2906    1937 
   Size:        969    4594
 
Acc65I G'GTAC_C

Cuts at:   2858    2884    2858 
   Size:         26    5537
 
AclI AA'CG_TT

Cuts at:   1563    3690    4063    1563 
   Size:       2127     373    3063

  Fragments arranged by size: 

                3063    2127     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3626    5010    5278    1632 
   Size:       1994    1384     268    1917

  Fragments arranged by size: 

                1994    1917    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5366
 
AflIII A'CryG_T

Cuts at:   1203    4462    5439    1203 
   Size:       3259     977    1327

  Fragments arranged by size: 

                3259    1327     977
 
AgeI A'CCGG_T

Cuts at:   4394    4394 
   Size:       5563
 
AhdI GACnn_n'nnGTC

Cuts at:   4292    4292 
   Size:       5563
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5563
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5563
 
AlwNI CAG_nnn'CTG

Cuts at:   2443    2641    2713    3306    4878    2443 
   Size:        198      72     593    1572    3128

  Fragments arranged by size: 

                3128    1572     593     198      72
 
ApaLI G'TGCA_C

Cuts at:   2722    3620    4776    2722 
   Size:        898    1156    3509

  Fragments arranged by size: 

                3509    1156     898
 
ApoI r'AATT_y

Cuts at:   1493    2872    3098    3250    5359    1493 
   Size:       1379     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697    1379     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4117     160 
   Size:        872    3085    1606

  Fragments arranged by size: 

                3085    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5563
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2083    2379    2888    1870 
   Size:         73     140     296     509    4545

  Fragments arranged by size: 

                4545     509     296     140      73
 
BamHI G'GATC_C

Cuts at:   2893    3260    2893 
   Size:        367    5196
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2078    2858    2884    4339     618 
   Size:        325     217     627     291     780      26    1455    1842

  Fragments arranged by size: 

                1842    1455     780     627     325     291     217      26
            
 
BanII G_rGCy'C

Cuts at:   1391    2093    2234    2472    2882    1391 
   Size:        702     141     238     410    4072

  Fragments arranged by size: 

                4072     702     410     238     141
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4592
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2817    2851    3754    3788    1903 
   Size:         34     880      34     903      34    3678

  Fragments arranged by size: 

                3678     903     880      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3455    4665    3455 
   Size:       1210    4353
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       5563
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2238    2622 
   Size:        107     122      71     631     605     337     229     384

Cuts at:   2622    4174     136 
   Size:       1552    1525

  Fragments arranged by size: 

                1552    1525     631     605     384     337     229     122
                 107      71
 
BglII A'GATC_T

Cuts at:   2173    5558    2173 
   Size:       3385    2178
 
BlpI GC'TnA_GC

Cuts at:   2245    2327    2980    2245 
   Size:         82     653    4828

  Fragments arranged by size: 

                4828     653      82
 
Bme1580I G_kGCm'C

Cuts at:   1790    2083    2487    2726    3624    4780    1790 
   Size:        293     404     239     898    1156    2573

  Fragments arranged by size: 

                2573    1156     898     404     293     239
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5563
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2431    3294    4252 
   Size:        416      53      83     399      24    1014     863     958

Cuts at:   4252     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1014     958     863     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5563
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2176    2176 
   Size:       5563
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2078    2342    2678    2684    4223    1239 
   Size:        839     264     336       6    1539    2579

  Fragments arranged by size: 

                2579    1539     839     336     264       6
 
Bpu10I CC'TnA_GC

Cuts at:   2106    2106 
   Size:       5563
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3688    4553    4851    3688 
   Size:        865     298    4400

  Fragments arranged by size: 

                4400     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4226     882 
   Size:        617    2727    2219

  Fragments arranged by size: 

                2727    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3354     493 
   Size:        711    2150    2702

  Fragments arranged by size: 

                2702    2150     711
 
BsaBI GATnn'nnATC

Cuts at:   2172    5557    2172 
   Size:       3385    2178
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3370    3752    4387     275 
   Size:         53      83     186     936    1837     382     635    1451

  Fragments arranged by size: 

                1837    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   2781    3996    4394    4668    4815    5226    2781 
   Size:       1215     398     274     147     411    3118

  Fragments arranged by size: 

                3118    1215     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5338

  Fragments arranged by size: 

                5338     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2075    2480    2702    2705    2768    2864    1186 
   Size:        889     405     222       3      63      96    3885

  Fragments arranged by size: 

                3885     889     405     222      96      63       3
 
BseYI C'CCAG_C

Cuts at:   2180    2331    4766    5262    2180 
   Size:        151    2435     496    2481

  Fragments arranged by size: 

                2481    2435     496     151
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4990
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3013    3774    4802    1540 
   Size:        275     186    1012     761    1028    2301

  Fragments arranged by size: 

                2301    1028    1012     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2726    3624    3709    4780    2726 
   Size:        898      85    1071    3509

  Fragments arranged by size: 

                3509    1071     898      85
 
BsmI GAATG_Cn'

Cuts at:   3074    3167    3074 
   Size:         93    5470
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2083    2093    2234    2472    2487    2726    2882 
   Size:        399     293      10     141     238      15     239     156

Cuts at:   2882    3624    3709    4780    1391 
   Size:        742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     399     293     239     238     156
                 141      85      15      10
 
BspEI T'CCGG_A

Cuts at:   2781    5226    2781 
   Size:       2445    3118
 
BspHI T'CATG_A

Cuts at:   2113    3452    5182    2113 
   Size:       1339    1730    2494

  Fragments arranged by size: 

                2494    1730    1339
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2918     844 
   Size:       2074    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3450    1394 
   Size:        429    1627    3507

  Fragments arranged by size: 

                3507    1627     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4058    4232      65 
   Size:       1183    2810     174    1396

  Fragments arranged by size: 

                2810    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2726    4207    4394    1700 
   Size:        110     916    1481     187    2869

  Fragments arranged by size: 

                2869    1481     916     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5563
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5563
 
BssSI C'ACGA_G

Cuts at:   3623    4635    3623 
   Size:       1012    4551
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2713     794 
   Size:       1919    3644
 
BstBI TT'CG_AA

Cuts at:   4367    4367 
   Size:       5563
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5563
 
BstYI r'GATC_y

Cuts at:   1972    2173    2493    2893    3260    3645    3662    5114    5200 
   Size:        201     320     400     367     385      17    1452      86

Cuts at:   5200    5212    5558    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1452     400     385     367     346     320     201
                  86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2144    2415    2488     513 
   Size:        552    1079     271      73    3588

  Fragments arranged by size: 

                3588    1079     552     271      73
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4824

  Fragments arranged by size: 

                4824     683      56
 
BtsI GCAGTG_nn'

Cuts at:   3079    3873    3893    3079 
   Size:        794      20    4749

  Fragments arranged by size: 

                4749     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5563
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    5528
 
DraI TTT'AAA

Cuts at:   2867    2867 
   Size:       5563
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4570     809 
   Size:        507    3254    1802

  Fragments arranged by size: 

                3254    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2010    2285    2728 
   Size:         54    1425      50     275      18     180     275     443

Cuts at:   2728    3010    3899    5528       8 
   Size:        282     889    1629      43

  Fragments arranged by size: 

                1629    1425     889     443     282     275     275     180
                  54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3010    1537 
   Size:        275    1198    4090

  Fragments arranged by size: 

                4090    1198     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2385    3493    1353 
   Size:       1032    1108    3423

  Fragments arranged by size: 

                3423    1108    1032
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2699    4150    4524    4670     387 
   Size:       1064     138    1110    1451     374     146    1280

  Fragments arranged by size: 

                1451    1280    1110    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2078    2342    2678    2684    3626    4223    5010 
   Size:        393     446     264     336       6     942     597     787

Cuts at:   5010    5278    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524     942     787     597     446     393     336     268
                 264       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2060    2060 
   Size:       5563
 
EcoO109I rG'GnC_Cy

Cuts at:   2373    2458    2373 
   Size:         85    5478
 
EcoRI G'AATT_C

Cuts at:   2872    3250    2872 
   Size:        378    5185
 
FspI TGC'GCA

Cuts at:   4069    4069 
   Size:       5563
 
HaeII r_GCGC'y

Cuts at:   1678    4710    1678 
   Size:       3032    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    1999    2031    4204    4236    4278 
   Size:         32     135      32     427      32    2173      32      42

Cuts at:   4278    4310    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    2173     427     135      42      32      32      32
                  32      32
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2907    3153     669 
   Size:        988     281     969     246    3079

  Fragments arranged by size: 

                3079     988     969     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5563
 
HpaI GTT'AAC

Cuts at:   3153    3153 
   Size:       5563
 
KpnI G_GTAC'C

Cuts at:   2862    2888    2862 
   Size:         26    5537
 
MfeI C'AATT_G

Cuts at:   3162    3162 
   Size:       5563
 
MluI A'CGCG_T

Cuts at:   5439    5439 
   Size:       5563
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4677    4861    1305 
   Size:       3372     184    2007

  Fragments arranged by size: 

                3372    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2012      10 
   Size:         54    1425     343     180    3561

  Fragments arranged by size: 

                3561    1425     343     180      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3521    3880    4039     518 
   Size:        648    2355     359     159    2042

  Fragments arranged by size: 

                2355    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2146    2249    2350    2572    2713    2797    2841    2979 
   Size:        540     103     101     222     141      84      44     138

Cuts at:   2979    3656    4804    5049    1606 
   Size:        677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     677     540     245     222     141     138
                 103     101      84      44
 
NcoI C'CATG_G

Cuts at:    513    1065    2488     513 
   Size:        552    1423    3588

  Fragments arranged by size: 

                3588    1423     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5563
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5563
 
NotI GC'GGCC_GC

Cuts at:   3010    3010 
   Size:       5563
 
NspI r_CATG'y

Cuts at:   1132    1229    2921    4466    1132 
   Size:         97    1692    1545    2229

  Fragments arranged by size: 

                2229    1692    1545      97
 
PasI CC'CwG_GG

Cuts at:   1348    2234    1348 
   Size:        886    4677
 
PciI A'CATG_T

Cuts at:   4462    4462 
   Size:       5563
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2416    1108 
   Size:       1308    4255
 
PmeI GTTT'AAAC

Cuts at:   2867    2867 
   Size:       5563
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3632    3664    5145    5177    3632 
   Size:         32    1481      32    4018

  Fragments arranged by size: 

                4018    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2458    2458 
   Size:       5563
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5563
 
PsiI TTA'TAA

Cuts at:   3133    3133 
   Size:       5563
 
PstI C_TGCA'G

Cuts at:    830    2622    2709    2718    2915     830 
   Size:       1792      87       9     197    3478

  Fragments arranged by size: 

                3478    1792     197      87       9
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5563
 
PvuII CAG'CTG

Cuts at:   2713    2713 
   Size:       5563
 
RsrII CG'GwC_CG

Cuts at:   2784    2784 
   Size:       5563
 
SacII CC_GC'GG

Cuts at:   2147    2147 
   Size:       5563
 
SalI G'TCGA_C

Cuts at:   1936    2905    1936 
   Size:        969    4594
 
SanDI GG'GwC_CC

Cuts at:   2458    2458 
   Size:       5563
 
SbfI CC_TGCA'GG

Cuts at:   2622    2915    2622 
   Size:        293    5270
 
ScaI AGT'ACT

Cuts at:   3811    5523    3811 
   Size:       1712    3851
 
SfcI C'TryA_G

Cuts at:    826    1045    2618    2705    2714    2911    4046    4727    4918 
   Size:        219    1573      87       9     197    1135     681     191

Cuts at:   4918     826 
   Size:       1471

  Fragments arranged by size: 

                1573    1471    1135     681     219     197     191      87
                   9
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5563
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5563
 
SmaI CCC'GGG

Cuts at:   1872    2085    2381    2890    1872 
   Size:        213     296     509    4545

  Fragments arranged by size: 

                4545     509     296     213
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3667    4568    4830     820 
   Size:        197     926    1724     901     262    1553

  Fragments arranged by size: 

                1724    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3354     493 
   Size:       2861    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5563
 
SphI G_CATG'C

Cuts at:   1132    2921    1132 
   Size:       1789    3774
 
SspI AAT'ATT

Cuts at:      5      52    3487       5 
   Size:         47    3435    2081

  Fragments arranged by size: 

                3435    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5563
 
StyI C'CwwG_G

Cuts at:    513    1065    2488    3002     513 
   Size:        552    1423     514    3074

  Fragments arranged by size: 

                3074    1423     552     514
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2450    2517    2801    3590    3607    3760    3945 
   Size:       1255     870      67     284     789      17     153     185

Cuts at:   3945    4390     325 
   Size:        445    1498

  Fragments arranged by size: 

                1498    1255     870     789     445     284     185     153
                  67      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3809    5521      96 
   Size:        274      80      33      51    1443    1832    1712     138

  Fragments arranged by size: 

                1832    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2492    2524    5523    5555    2492 
   Size:         32    2999      32    2500

  Fragments arranged by size: 

                2999    2500      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5563
 
XbaI T'CTAG_A

Cuts at:   2899    2899 
   Size:       5563
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5563
 
XmaI C'CCGG_G

Cuts at:   1870    2083    2379    2888    1870 
   Size:        213     296     509    4545

  Fragments arranged by size: 

                4545     509     296     213
 
XmnI GAAnn'nnTTC

Cuts at:   2579    3692    2579 
   Size:       1113    4450
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3371     276 
   Size:         53      83     186     936    1837    2468

  Fragments arranged by size: 

                2468    1837     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI
  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I   DraIII
  EcoICRI    EcoRV     FalI     FseI    FspAI     KasI     NaeI     NarI
   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI   PspOMI    PspXI
     PsrI     SacI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
     XcmI
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