(Circular) (Six-base) MAPSORT of: pFN21AE1989.seq Check: 6160 from: 1 to: 6400 pFN21AE1989 6400 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4210 278 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2808 3564 3743 1937 Size: 871 756 179 4594 Fragments arranged by size: 4594 871 756 179 Acc65I G'GTAC_C Cuts at: 3721 3721 Size: 6400 AclI AA'CG_TT Cuts at: 1563 4527 4900 1563 Size: 2964 373 3063 Fragments arranged by size: 3063 2964 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2998 4463 5847 6115 1632 Size: 1366 1465 1384 268 1917 Fragments arranged by size: 1917 1465 1384 1366 268 AfeI AGC'GCT Cuts at: 2819 2930 3479 2819 Size: 111 549 5740 Fragments arranged by size: 5740 549 111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6203 AflIII A'CryG_T Cuts at: 1203 2726 5299 6276 1203 Size: 1523 2573 977 1327 Fragments arranged by size: 2573 1523 1327 977 AgeI A'CCGG_T Cuts at: 5231 5231 Size: 6400 AhdI GACnn_n'nnGTC Cuts at: 5129 5129 Size: 6400 AleI CACnn'nnGTG Cuts at: 1166 2299 2870 2957 1166 Size: 1133 571 87 4609 Fragments arranged by size: 4609 1133 571 87 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2039 2317 2544 2975 3653 1184 Size: 855 278 227 431 678 3931 Fragments arranged by size: 3931 855 678 431 278 227 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2307 2339 2307 Size: 32 6368 AlwNI CAG_nnn'CTG Cuts at: 4143 5715 4143 Size: 1572 4828 ApaLI G'TGCA_C Cuts at: 2143 2222 2302 4457 5613 2143 Size: 79 80 2155 1156 2930 Fragments arranged by size: 2930 2155 1156 80 79 ApoI r'AATT_y Cuts at: 1493 3709 3935 4087 6196 1493 Size: 2216 226 152 2109 1697 Fragments arranged by size: 2216 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4954 160 Size: 872 3922 1606 Fragments arranged by size: 3922 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 AvaI C'yCGr_G Cuts at: 1870 1943 2402 2542 2551 3725 1870 Size: 73 459 140 9 1174 4545 Fragments arranged by size: 4545 1174 459 140 73 9 BamHI G'GATC_C Cuts at: 2745 3730 4097 2745 Size: 985 367 5048 Fragments arranged by size: 5048 985 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2438 2528 2537 2623 2966 Size: 325 217 627 651 90 9 86 343 Cuts at: 2966 3350 3721 5176 618 Size: 384 371 1455 1842 Fragments arranged by size: 1842 1455 651 627 384 371 343 325 217 90 86 9 BanII G_rGCy'C Cuts at: 1391 2485 3719 1391 Size: 1094 1234 4072 Fragments arranged by size: 4072 1234 1094 BbeI G_GCGC'C Cuts at: 2442 2442 Size: 6400 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3388 928 Size: 971 1489 3940 Fragments arranged by size: 3940 1489 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2134 2168 4591 4625 1903 Size: 34 50 34 113 34 2423 34 3678 Fragments arranged by size: 3678 2423 113 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4292 5502 4292 Size: 1210 5190 BclI T'GATC_A Cuts at: 1548 3570 1548 Size: 2022 4378 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2069 2556 3101 Size: 107 122 71 631 605 397 487 545 Cuts at: 3101 3134 3143 5011 136 Size: 33 9 1868 1525 Fragments arranged by size: 1868 1525 631 605 545 487 397 122 107 71 33 9 BglII A'GATC_T Cuts at: 2448 6395 2448 Size: 3947 2453 BlpI GC'TnA_GC Cuts at: 3197 3817 3197 Size: 620 5780 Bme1580I G_kGCm'C Cuts at: 1790 2147 2226 2306 2540 2969 3420 3524 4461 Size: 357 79 80 234 429 451 104 937 Cuts at: 4461 5617 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 937 451 429 357 234 104 80 79 BmgBI CAC'GTC Cuts at: 1318 3288 3606 1318 Size: 1970 318 4112 Fragments arranged by size: 4112 1970 318 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2068 3481 4131 Size: 416 53 83 399 24 651 1413 650 Cuts at: 4131 5089 442 Size: 958 1753 Fragments arranged by size: 1753 1413 958 651 650 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3257 1056 Size: 2201 4199 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2950 3124 2950 Size: 174 6226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2609 2888 3389 5060 1239 Size: 1370 279 501 1671 2579 Fragments arranged by size: 2579 1671 1370 501 279 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2603 4525 5390 5688 2603 Size: 1922 865 298 3315 Fragments arranged by size: 3315 1922 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3683 5063 882 Size: 617 2184 1380 2219 Fragments arranged by size: 2219 2184 1380 617 BsaAI yAC'GTr Cuts at: 493 1204 2799 4191 493 Size: 711 1595 1392 2702 Fragments arranged by size: 2702 1595 1392 711 BsaBI GATnn'nnATC Cuts at: 6394 6394 Size: 6400 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2439 3393 4207 4589 Size: 53 83 186 936 906 954 814 382 Cuts at: 4589 5224 275 Size: 635 1451 Fragments arranged by size: 1451 954 936 906 814 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4833 5231 5505 5652 6063 4833 Size: 398 274 147 411 5170 Fragments arranged by size: 5170 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3467 3497 1178 Size: 30 165 30 2064 30 4081 Fragments arranged by size: 4081 2064 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2735 1186 Size: 1549 4851 BseYI C'CCAG_C Cuts at: 5603 6099 5603 Size: 496 5904 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2270 2968 2981 3623 1163 Size: 573 534 698 13 642 3940 Fragments arranged by size: 3940 698 642 573 534 13 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3147 3850 4611 5639 1540 Size: 275 186 1146 703 761 1028 2301 Fragments arranged by size: 2301 1146 1028 761 703 275 186 BsiHKAI G_wGCw'C Cuts at: 2147 2226 2258 2285 2306 2424 2485 2595 2904 Size: 79 32 27 21 118 61 110 309 Cuts at: 2904 3408 3471 4461 4546 5617 2147 Size: 504 63 990 85 1071 2930 Fragments arranged by size: 2930 1071 990 504 309 118 110 85 79 63 61 32 27 21 BsmI GAATG_Cn' Cuts at: 3911 4004 3911 Size: 93 6307 BsmBI CGTCTCn'nnnn_ Cuts at: 2717 2717 Size: 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2226 2258 2285 2306 2424 2485 Size: 399 357 79 32 27 21 118 61 Cuts at: 2485 2540 2595 2904 2969 3408 3420 3471 3524 Size: 55 55 309 65 439 12 51 53 Cuts at: 3524 3719 4461 4546 5617 1391 Size: 195 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 357 309 195 118 85 79 65 61 55 55 53 51 32 27 21 12 BspEI T'CCGG_A Cuts at: 6063 6063 Size: 6400 BspHI T'CATG_A Cuts at: 2376 2997 3336 3582 4289 6019 2376 Size: 621 339 246 707 1730 2757 Fragments arranged by size: 2757 1730 707 621 339 246 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2692 3201 3755 844 Size: 1848 509 554 3489 Fragments arranged by size: 3489 1848 554 509 BsrBI CCG'CTC Cuts at: 1394 1823 2337 2836 3201 3262 3373 4287 1394 Size: 429 514 499 365 61 111 914 3507 Fragments arranged by size: 3507 914 514 499 429 365 111 61 BsrDI GCAATG_nn' Cuts at: 65 1248 4895 5069 65 Size: 1183 3647 174 1396 Fragments arranged by size: 3647 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 3071 3142 3302 5044 5231 1700 Size: 110 258 1003 71 160 1742 187 2869 Fragments arranged by size: 2869 1742 1003 258 187 160 110 71 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6400 BssHII G'CGCG_C Cuts at: 1925 2350 2352 2354 2735 1925 Size: 425 2 2 381 5590 Fragments arranged by size: 5590 425 381 2 2 BssSI C'ACGA_G Cuts at: 3089 3481 3643 4460 5472 3089 Size: 392 162 817 1012 4017 Fragments arranged by size: 4017 1012 817 392 162 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6400 BstBI TT'CG_AA Cuts at: 5204 5204 Size: 6400 BstEII G'GTnAC_C Cuts at: 1184 2945 1184 Size: 1761 4639 BstXI CCAn_nnnn'nTGG Cuts at: 2185 2803 2185 Size: 618 5782 BstYI r'GATC_y Cuts at: 1972 2448 2745 3618 3730 4097 4482 4499 5951 Size: 476 297 873 112 367 385 17 1452 Cuts at: 5951 6037 6049 6395 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 873 476 385 367 346 297 112 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2080 2110 2434 2868 3171 3696 513 Size: 552 1015 30 324 434 303 525 3217 Fragments arranged by size: 3217 1015 552 525 434 324 303 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2137 2872 3418 487 Size: 683 56 911 735 546 3469 Fragments arranged by size: 3469 911 735 683 546 56 BtsI GCAGTG_nn' Cuts at: 2258 2602 3496 3916 4710 4730 2258 Size: 344 894 420 794 20 3928 Fragments arranged by size: 3928 894 794 420 344 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6400 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6365 DraI TTT'AAA Cuts at: 3704 3704 Size: 6400 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3560 5407 809 Size: 507 2244 1847 1802 Fragments arranged by size: 2244 1847 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2458 3021 3126 Size: 54 1425 50 275 18 628 563 105 Cuts at: 3126 3144 3168 3300 3510 3847 4736 6365 8 Size: 18 24 132 210 337 889 1629 43 Fragments arranged by size: 1629 1425 889 628 563 337 275 210 132 105 54 50 43 24 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3144 3847 1537 Size: 275 1332 703 4090 Fragments arranged by size: 4090 1332 703 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2999 3269 3323 4330 1353 Size: 1646 270 54 1007 3423 Fragments arranged by size: 3423 1646 1007 270 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4987 5361 5507 387 Size: 1064 138 3398 374 146 1280 Fragments arranged by size: 3398 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2483 2483 Size: 6400 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2609 2888 2998 3389 4463 5060 5847 Size: 393 977 279 110 391 1074 597 787 Cuts at: 5847 6115 1239 Size: 268 1524 Fragments arranged by size: 1524 1074 977 787 597 393 391 279 268 110 EcoRI G'AATT_C Cuts at: 3709 4087 3709 Size: 378 6022 FseI GG_CCGG'CC Cuts at: 2072 2072 Size: 6400 FspI TGC'GCA Cuts at: 3386 4906 3386 Size: 1520 4880 FspAI rTGC'GCAy Cuts at: 3386 3386 Size: 6400 HaeII r_GCGC'y Cuts at: 1678 2022 2410 2442 2568 2821 2932 3260 3481 Size: 344 388 32 126 253 111 328 221 Cuts at: 3481 5547 1678 Size: 2066 2531 Fragments arranged by size: 2531 2066 388 344 328 253 221 126 111 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5041 5073 5115 5147 1373 Size: 32 135 32 3469 32 42 32 2626 Fragments arranged by size: 3469 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3744 3990 669 Size: 988 281 1806 246 3079 Fragments arranged by size: 3079 1806 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6400 HpaI GTT'AAC Cuts at: 3990 3990 Size: 6400 KasI G'GCGC_C Cuts at: 2438 2438 Size: 6400 KpnI G_GTAC'C Cuts at: 3725 3725 Size: 6400 MfeI C'AATT_G Cuts at: 3999 3999 Size: 6400 MluI A'CGCG_T Cuts at: 2726 6276 2726 Size: 3550 2850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5514 5698 1305 Size: 4209 184 2007 Fragments arranged by size: 4209 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3023 3170 3512 10 Size: 54 1425 343 1191 147 342 2898 Fragments arranged by size: 2898 1425 1191 343 342 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2299 2870 2957 3335 4358 4717 4876 Size: 648 1133 571 87 378 1023 359 159 Cuts at: 4876 518 Size: 2042 Fragments arranged by size: 2042 1133 1023 648 571 378 359 159 87 MspA1I CmG'CkG Cuts at: 1606 2044 2140 2239 3238 3816 4493 5641 5886 Size: 438 96 99 999 578 677 1148 245 Cuts at: 5886 1606 Size: 2120 Fragments arranged by size: 2120 1148 999 677 578 438 245 99 96 NaeI GCC'GGC Cuts at: 2070 3073 3144 3304 2070 Size: 1003 71 160 5166 Fragments arranged by size: 5166 1003 160 71 NarI GG'CG_CC Cuts at: 2439 2439 Size: 6400 NcoI C'CATG_G Cuts at: 513 1065 2868 3171 513 Size: 552 1803 303 3742 Fragments arranged by size: 3742 1803 552 303 NdeI CA'TA_TG Cuts at: 387 387 Size: 6400 NgoMIV G'CCGG_C Cuts at: 2068 3071 3142 3302 2068 Size: 1003 71 160 5166 Fragments arranged by size: 5166 1003 160 71 NheI G'CTAG_C Cuts at: 1052 3253 1052 Size: 2201 4199 NotI GC'GGCC_GC Cuts at: 3847 3847 Size: 6400 NspI r_CATG'y Cuts at: 1132 1229 2815 3547 3758 5303 1132 Size: 97 1586 732 211 1545 2229 Fragments arranged by size: 2229 1586 1545 732 211 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6400 PciI A'CATG_T Cuts at: 5299 5299 Size: 6400 PflMI CCAn_nnn'nTGG Cuts at: 1108 3036 1108 Size: 1928 4472 PfoI T'CCnGG_A Cuts at: 2103 2904 2103 Size: 801 5599 PmeI GTTT'AAAC Cuts at: 3704 3704 Size: 6400 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4469 4501 5982 6014 4469 Size: 32 1481 32 4855 Fragments arranged by size: 4855 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6400 PsiI TTA'TAA Cuts at: 3970 3970 Size: 6400 PstI C_TGCA'G Cuts at: 830 2523 3752 830 Size: 1693 1229 3478 Fragments arranged by size: 3478 1693 1229 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6400 SacI G_AGCT'C Cuts at: 2485 2485 Size: 6400 SalI G'TCGA_C Cuts at: 1936 3742 1936 Size: 1806 4594 SapI GCTCTTCn'nnn_ Cuts at: 3269 3323 3269 Size: 54 6346 SbfI CC_TGCA'GG Cuts at: 3752 3752 Size: 6400 ScaI AGT'ACT Cuts at: 3116 4648 6360 3116 Size: 1532 1712 3156 Fragments arranged by size: 3156 1712 1532 SexAI A'CCwGG_T Cuts at: 2753 3056 2753 Size: 303 6097 SfcI C'TryA_G Cuts at: 826 1045 2519 3748 4883 5564 5755 826 Size: 219 1474 1229 1135 681 191 1471 Fragments arranged by size: 1474 1471 1229 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3101 3134 3143 3101 Size: 33 9 6358 Fragments arranged by size: 6358 33 9 SfoI GGC'GCC Cuts at: 2440 2440 Size: 6400 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6400 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6400 SmaI CCC'GGG Cuts at: 1872 2544 2553 3727 1872 Size: 672 9 1174 4545 Fragments arranged by size: 4545 1174 672 9 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 4504 5405 5667 820 Size: 197 926 675 1886 901 262 1553 Fragments arranged by size: 1886 1553 926 901 675 262 197 SnaBI TAC'GTA Cuts at: 493 4191 493 Size: 3698 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6400 SphI G_CATG'C Cuts at: 1132 3758 1132 Size: 2626 3774 SrfI GCCC'GGGC Cuts at: 2553 2553 Size: 6400 SspI AAT'ATT Cuts at: 5 52 4324 5 Size: 47 4272 2081 Fragments arranged by size: 4272 2081 47 StuI AGG'CCT Cuts at: 1517 2214 3219 3458 1517 Size: 697 1005 239 4459 Fragments arranged by size: 4459 1005 697 239 StyI C'CwwG_G Cuts at: 513 1065 2868 3171 3839 513 Size: 552 1803 303 668 3074 Fragments arranged by size: 3074 1803 668 552 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3374 3475 4427 4444 4597 4782 5227 Size: 1255 1794 101 952 17 153 185 445 Cuts at: 5227 325 Size: 1498 Fragments arranged by size: 1794 1498 1255 952 445 185 153 101 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3114 4646 6358 Size: 274 80 33 51 1443 1137 1532 1712 Cuts at: 6358 96 Size: 138 Fragments arranged by size: 1712 1532 1443 1137 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3467 3499 6360 6392 3467 Size: 32 2861 32 3475 Fragments arranged by size: 3475 2861 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6400 XbaI T'CTAG_A Cuts at: 3736 3736 Size: 6400 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2875 3163 3169 2875 Size: 288 6 6106 Fragments arranged by size: 6106 288 6 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6400 XmaI C'CCGG_G Cuts at: 1870 2542 2551 3725 1870 Size: 672 9 1174 4545 Fragments arranged by size: 4545 1174 672 9 XmnI GAAnn'nnTTC Cuts at: 2384 4529 2384 Size: 2145 4255 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4208 276 Size: 53 83 186 936 2674 2468 Fragments arranged by size: 2674 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SwaI