(Linear) (Six-base) MAPSORT of: KIEE6585.seq Check: 2495 from: 1 to: 477 KIEE6585.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 58 85 172 187 421 477 Size: 58 27 87 15 234 56 Fragments arranged by size: 234 87 58 56 27 15 AleI CACnn'nnGTG Cuts at: 0 67 445 477 Size: 67 378 32 Fragments arranged by size: 378 67 32 AlwNI CAG_nnn'CTG Cuts at: 0 125 209 284 458 477 Size: 125 84 75 174 19 Fragments arranged by size: 174 125 84 75 19 ApaLI G'TGCA_C Cuts at: 0 287 477 Size: 287 190 Bme1580I G_kGCm'C Cuts at: 0 291 477 Size: 291 186 BsaI GGTCTCn'nnnn_ Cuts at: 0 400 477 Size: 400 77 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 28 477 Size: 28 449 BseYI C'CCAG_C Cuts at: 0 393 477 Size: 393 84 BsiHKAI G_wGCw'C Cuts at: 0 291 477 Size: 291 186 Bsp1286I G_dGCh'C Cuts at: 0 291 477 Size: 291 186 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 43 58 85 172 187 319 421 477 Size: 43 15 27 87 15 132 102 56 Fragments arranged by size: 132 102 87 56 43 27 15 15 BsrGI T'GTAC_A Cuts at: 0 449 477 Size: 449 28 BstAPI GCAn_nnn'nTGC Cuts at: 0 92 236 477 Size: 92 144 241 Fragments arranged by size: 241 144 92 BstXI CCAn_nnnn'nTGG Cuts at: 0 361 477 Size: 361 116 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 130 165 477 Size: 130 35 312 Fragments arranged by size: 312 130 35 EcoNI CCTnn'n_nnAGG Cuts at: 0 39 168 417 477 Size: 39 129 249 60 Fragments arranged by size: 249 129 60 39 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 392 477 Size: 392 85 MslI CAynn'nnrTG Cuts at: 0 67 196 445 477 Size: 67 129 249 32 Fragments arranged by size: 249 129 67 32 MspA1I CmG'CkG Cuts at: 0 122 206 266 455 477 Size: 122 84 60 189 22 Fragments arranged by size: 189 122 84 60 22 NspI r_CATG'y Cuts at: 0 254 477 Size: 254 223 PflMI CCAn_nnn'nTGG Cuts at: 0 53 477 Size: 53 424 PstI C_TGCA'G Cuts at: 0 43 82 304 421 477 Size: 43 39 222 117 56 Fragments arranged by size: 222 117 56 43 39 PvuII CAG'CTG Cuts at: 0 122 206 266 455 477 Size: 122 84 60 189 22 Fragments arranged by size: 189 122 84 60 22 SfcI C'TryA_G Cuts at: 0 39 78 168 225 300 417 477 Size: 39 39 90 57 75 117 60 Fragments arranged by size: 117 90 75 60 57 39 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 158 477 Size: 158 319 TatI w'GTAC_w Cuts at: 0 296 449 477 Size: 296 153 28 Fragments arranged by size: 296 153 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 30 477 Size: 30 447 Enzymes that do cut: AarI AleI AlwNI ApaLI Bme1580I BsaI BsaXI BseYI BsiHKAI Bsp1286I BspMI BsrGI BstAPI BstXI CspCI EcoNI MmeI MslI MspA1I NspI PflMI PstI PvuII SfcI TaqII TatI TstI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AlfI AloI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI