Restriction map for
pFN21AE1582
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# NO CUT ENZYMES ( 189 enzymes) #
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AatII AccI AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI AhdI AlwI AlwNI AocI
Aor51HI ApaI ApoI AscI AseI
AsnI Asp700I AspEI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglI
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsiEI BsiWI BsmAI
BsmBI BsmFI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
ClaI CpoI DdeI DraI DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HindIII HpaI
HphI KasI Ksp632I KspI MaeI
MaeII MaeIII MamI MboII MfeI
MluI MluNI MroI MscI MseI
MslI MunI MvnI NarI NciI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp45I Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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Acc65I 70 GGTACC G^GTACC
AfaI 71 GTAC GT^AC
Alw44I 59 GTGCAC G^TGCAC
ApaLI 59 GTGCAC G^TGCAC
Asp718I 70 GGTACC G^GTACC
AvaII 231 GGTCC G^G[AT]CC
BanI 70 GGTACC G^G[CT][GA]CC
BanII 82 GAGCTC G[GA]GC[CT]^C
BglII 203 AGATCT A^GATCT
BsaJI 41 CCATGG C^C[ACGT][ACGT]GG
BsgI 36 CTGCAC GTGCAG(16/14)
BsiYI 163 CCCAAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 163 CCCAAGCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 261 GAATGC GAATGC(1/-1)
BsrFI 197 GCCGGC [GA]^CCGG[CT]
BsrI 156 CCAGT ACTGG(1/-1)
BstF5I 177 CATCC GGATG(2/0)
BstYI 203 AGATCT [GA]^GATC[CT]
Cac8I 197 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 197 GCCGGC [GA]^CCGG[CT]
DpnII 204 GATC ^GATC
DraII 5 AGGGCCT [GA]G^G[ACGT]CC[CT]
DsaI 41 CCATGG C^C[GA][CT]GG
EcoO109I 5 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 41 CCATGG C^C[AT][AT]GG
FokI 177 CATCC GGATG(9/13)
HapII 198 CCGG C^CGG
HincII 133 GTTGAC GT[CT]^[GA]AC
HindII 133 GTTGAC GT[CT]^[GA]AC
HinfI 114 GATTC G^A[ACGT]TC
HpaII 198 CCGG C^CGG
KpnI 70 GGTACC GGTAC^C
MboI 204 GATC ^GATC
MflI 203 AGATCT [GA]^GATC[CT]
MspA1I 14 CAGCTG C[AC]G^C[GT]G
MspI 198 CCGG C^CGG
MwoI 152 GCCCCCAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 197 GCCGGC GCC^GGC
NcoI 41 CCATGG C^CATGG
NdeII 204 GATC ^GATC
NgoMIV 197 GCCGGC G^CCGGC
NlaIII 42 CATG CATG^
PvuII 14 CAGCTG CAG^CTG
RsaI 71 GTAC GT^AC
SacI 82 GAGCTC GAGCT^C
Sau3AI 204 GATC ^GATC
StyI 41 CCATGG C^C[AT][AT]GG
TfiI 114 GATTC G^A[AT]TC
TspRI 157 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 203 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 8 enzymes) #
###################################
AspHI 59 GTGCAC G[AT]GC[AT]^C
82 GAGCTC
BsiHKAI 59 GTGCAC G[AT]GC[AT]^C
82 GAGCTC
BssKI 107 CCTGG ^CC[ACGT]GG
169 CCAGG
BstNI 107 CCTGG CC^[AT]GG
169 CCAGG
EcoRII 107 CCTGG ^CC[AT]GG
169 CCAGG
MvaI 107 CCTGG CC^[AT]GG
169 CCAGG
NlaIV 70 GGTACC GG[ACGT]^[ACGT]CC
230 GGGTCC
ScrFI 107 CCTGG CC^[ACGT]GG
169 CCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AluI BbvI BmyI Bsp1286I Cfr13I
Fnu4HI HaeIII ItaI MnlI Sau96I
TseI