Restriction map for
pFN21AE1623
##################################
# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BanI BanII BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmFI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HpaI KasI KpnI Ksp632I
KspI MamI MfeI MluI MluNI
MroI MscI MseI MslI MspA1I
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AfaI 124 GTAC GT^AC
AspHI 282 GTGCTC G[AT]GC[AT]^C
AvaI 62 CCCGGG C^[CT]CG[GA]G
AvaII 157 GGTCC G^G[AT]CC
BamHI 52 GGATCC G^GATCC
BfaI 200 CTAG C^TAG
BmyI 282 GTGCTC G[AGT]GC[ACT]^C
BsaI 6 GGTCTC GGTCTC(1/5)
BseRI 144 GAGGAG GAGGAG(10/8)
BsiHKAI 282 GTGCTC G[AT]GC[AT]^C
BsmI 371 GAATGC GAATGC(1/-1)
BsoBI 62 CCCGGG C^[CT]CG[GA]G
Bsp1286I 282 GTGCTC G[AGT]GC[ACT]^C
BspHI 92 TCATGA T^CATGA
BstF5I 237 CATCC GGATG(2/0)
BstYI 52 GGATCC [GA]^GATC[CT]
DdeI 161 CTCAG C^T[ACGT]AG
DsaI 11 CCGTGG C^C[GA][CT]GG
EcoT14I 48 CCTTGG C^C[AT][AT]GG
FokI 237 CATCC GGATG(9/13)
HaeIII 60 GGCC GG^CC
HindIII 103 AAGCTT A^AGCTT
HinfI 188 GATTC G^A[ACGT]TC
HphI 308 TCACC GGTGA(8/7)
MaeI 200 CTAG C^TAG
MaeII 324 ACGT A^CGT
MflI 52 GGATCC [GA]^GATC[CT]
MwoI 196 GCTACTAGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NlaIV 52 GGATCC GG[ACGT]^[ACGT]CC
NspI 134 ACATGC [GA]CATG^[CT]
PstI 182 CTGCAG CTGCA^G
RcaI 92 TCATGA T^CATGA
RsaI 124 GTAC GT^AC
ScaI 123 AGTACT AGT^ACT
SfcI 182 CTGCAG C^T[GA][CT]AG
SmaI 62 CCCGGG CCC^GGG
SpeI 199 ACTAGT A^CTAGT
StyI 48 CCTTGG C^C[AT][AT]GG
TfiI 188 GATTC G^A[AT]TC
Tsp45I 67 GTCAC ^GT[GC]AC
TspRI 342 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 52 GGATCC [GA]^GATC[CT]
XmaCI 62 CCCGGG C^CCGGG
XmaI 62 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AluI 104 AGCT AG^CT
380 AGCT
BpmI 141 CTGGAG CTGGAG(16/14)
253 CTGGAG
BsmAI 82 GAGAC GTCTC(1/5)
7 GTCTC
BsrI 121 CCAGT ACTGG(1/-1)
341 CCAGT
Cfr13I 60 GGCCC G^G[ACGT]CC
157 GGTCC
DpnII 53 GATC ^GATC
393 GATC
HapII 63 CCGG C^CGG
292 CCGG
HpaII 63 CCGG C^CGG
292 CCGG
MboI 53 GATC ^GATC
393 GATC
MboII 405 GAAGA GAAGA(8/7)
302 TCTTC
MspI 63 CCGG C^CGG
292 CCGG
NdeII 53 GATC ^GATC
393 GATC
Sau3AI 53 GATC ^GATC
393 GATC
Sau96I 60 GGCCC G^G[ACGT]CC
157 GGTCC
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AlwI BbvI BcnI BsaJI BsiYI
BslI BssKI BstNI EcoRII Fnu4HI
ItaI MaeIII MnlI MvaI NciI
NlaIII ScrFI SfaNI TseI