(Linear) (Six-base) MAPSORT of: KIEE5621.seq Check: 5533 from: 1 to: 1536 KIEE5621.orf With 182 enzymes: SgfI * December 5, 2014 13:03 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 511 1536 Size: 511 1025 AflIII A'CryG_T Cuts at: 0 581 1536 Size: 581 955 AleI CACnn'nnGTG Cuts at: 0 223 553 1536 Size: 223 330 983 Fragments arranged by size: 983 330 223 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 628 1536 Size: 628 908 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 719 751 1536 Size: 719 32 785 Fragments arranged by size: 785 719 32 AlwNI CAG_nnn'CTG Cuts at: 0 1033 1536 Size: 1033 503 ApoI r'AATT_y Cuts at: 0 1168 1536 Size: 1168 368 AvrII C'CTAG_G Cuts at: 0 1038 1536 Size: 1038 498 BanII G_rGCy'C Cuts at: 0 154 181 1118 1349 1536 Size: 154 27 937 231 187 Fragments arranged by size: 937 231 187 154 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 246 1536 Size: 246 1290 BciVI GTATCCnnnnn_n' Cuts at: 0 1339 1536 Size: 1339 197 BclI T'GATC_A Cuts at: 0 1370 1536 Size: 1370 166 BglII A'GATC_T Cuts at: 0 53 1536 Size: 53 1483 Bme1580I G_kGCm'C Cuts at: 0 1496 1536 Size: 1496 40 BmrI ACTGGGnnnn_n' Cuts at: 0 14 1536 Size: 14 1522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 421 481 670 779 1536 Size: 282 139 60 189 109 757 Fragments arranged by size: 757 282 189 139 109 60 Bpu10I CC'TnA_GC Cuts at: 0 1007 1536 Size: 1007 529 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1180 1536 Size: 1180 356 BsaI GGTCTCn'nnnn_ Cuts at: 0 928 1536 Size: 928 608 BsaWI w'CCGG_w Cuts at: 0 290 1536 Size: 290 1246 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 187 217 719 749 1536 Size: 187 30 502 30 787 Fragments arranged by size: 787 502 187 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1420 1536 Size: 1420 116 BseYI C'CCAG_C Cuts at: 0 258 328 777 1135 1536 Size: 258 70 449 358 401 Fragments arranged by size: 449 401 358 258 70 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 1046 1536 Size: 454 592 490 Fragments arranged by size: 592 490 454 BsiHKAI G_wGCw'C Cuts at: 0 181 1118 1349 1536 Size: 181 937 231 187 Fragments arranged by size: 937 231 187 181 Bsp1286I G_dGCh'C Cuts at: 0 154 181 1118 1349 1496 1536 Size: 154 27 937 231 147 40 Fragments arranged by size: 937 231 154 147 40 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 368 692 851 1400 1536 Size: 368 324 159 549 136 Fragments arranged by size: 549 368 324 159 136 BstAPI GCAn_nnn'nTGC Cuts at: 0 1033 1536 Size: 1033 503 BstYI r'GATC_y Cuts at: 0 53 365 740 1130 1536 Size: 53 312 375 390 406 Fragments arranged by size: 406 390 375 312 53 BtgI C'CryG_G Cuts at: 0 78 620 853 1121 1536 Size: 78 542 233 268 415 Fragments arranged by size: 542 415 268 233 78 BtsI GCAGTG_nn' Cuts at: 0 143 1536 Size: 143 1393 DraIII CAC_nnn'GTG Cuts at: 0 477 1485 1536 Size: 477 1008 51 Fragments arranged by size: 1008 477 51 EaeI y'GGCC_r Cuts at: 0 534 815 1536 Size: 534 281 721 Fragments arranged by size: 721 534 281 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 76 1066 1536 Size: 76 990 470 Fragments arranged by size: 990 470 76 EcoICRI GAG'CTC Cuts at: 0 179 1116 1347 1536 Size: 179 937 231 189 Fragments arranged by size: 937 231 189 179 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 282 421 481 511 670 779 1536 Size: 282 139 60 30 159 109 757 Fragments arranged by size: 757 282 159 139 109 60 30 EcoNI CCTnn'n_nnAGG Cuts at: 0 1037 1536 Size: 1037 499 EcoO109I rG'GnC_Cy Cuts at: 0 405 1514 1536 Size: 405 1109 22 Fragments arranged by size: 1109 405 22 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 441 1536 Size: 441 1095 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 175 207 732 764 853 885 1536 Size: 175 32 525 32 89 32 651 Fragments arranged by size: 651 525 175 89 32 32 32 HincII GTy'rAC Cuts at: 0 357 1536 Size: 357 1179 MfeI C'AATT_G Cuts at: 0 1247 1536 Size: 1247 289 MscI TGG'CCA Cuts at: 0 817 1536 Size: 817 719 MslI CAynn'nnrTG Cuts at: 0 97 223 553 1536 Size: 97 126 330 983 Fragments arranged by size: 983 330 126 97 MspA1I CmG'CkG Cuts at: 0 111 262 1536 Size: 111 151 1274 Fragments arranged by size: 1274 151 111 NcoI C'CATG_G Cuts at: 0 78 1121 1536 Size: 78 1043 415 Fragments arranged by size: 1043 415 78 NspI r_CATG'y Cuts at: 0 217 459 585 981 1536 Size: 217 242 126 396 555 Fragments arranged by size: 555 396 242 217 126 PasI CC'CwG_GG Cuts at: 0 1509 1536 Size: 1509 27 PciI A'CATG_T Cuts at: 0 581 1536 Size: 581 955 PflMI CCAn_nnn'nTGG Cuts at: 0 777 1536 Size: 777 759 PfoI T'CCnGG_A Cuts at: 0 368 1536 Size: 368 1168 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 719 751 1536 Size: 719 32 785 Fragments arranged by size: 785 719 32 PpuMI rG'GwC_Cy Cuts at: 0 1514 1536 Size: 1514 22 PstI C_TGCA'G Cuts at: 0 365 1081 1502 1536 Size: 365 716 421 34 Fragments arranged by size: 716 421 365 34 PvuII CAG'CTG Cuts at: 0 262 1536 Size: 262 1274 SacI G_AGCT'C Cuts at: 0 181 1118 1349 1536 Size: 181 937 231 187 Fragments arranged by size: 937 231 187 181 ScaI AGT'ACT Cuts at: 0 1440 1536 Size: 1440 96 SfcI C'TryA_G Cuts at: 0 361 918 1077 1498 1536 Size: 361 557 159 421 38 Fragments arranged by size: 557 421 361 159 38 SmlI C'TyrA_G Cuts at: 0 7 1159 1536 Size: 7 1152 377 Fragments arranged by size: 1152 377 7 SphI G_CATG'C Cuts at: 0 459 1536 Size: 459 1077 StyI C'CwwG_G Cuts at: 0 78 1038 1121 1517 1536 Size: 78 960 83 396 19 Fragments arranged by size: 960 396 83 78 19 TatI w'GTAC_w Cuts at: 0 1438 1536 Size: 1438 98 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 752 784 915 947 1536 Size: 752 32 131 32 589 Fragments arranged by size: 752 589 131 32 32 Enzymes that do cut: AcuI AflIII AleI AlfI AloI AlwNI ApoI AvrII BanII BbsI BciVI BclI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BstAPI BstYI BtgI BtsI DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MfeI MscI MslI MspA1I NcoI NspI PasI PciI PflMI PfoI PpiI PpuMI PstI PvuII SacI ScaI SfcI SmlI SphI StyI TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI ApaI ApaLI AscI AseI AsiSI AvaI BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI