################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BalI BamHI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MunI NaeI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 436 GTAGAC GT^[AC][GT]AC AcsI 617 AAATTC [GA]^AATT[CT] AlwI 534 GGATC GGATC(4/5) ApoI 617 AAATTC [GA]^AATT[CT] Asp700I 613 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 309 CCCGGG C^[CT]CG[GA]G BcgI 570 CGACACAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 185 GCCGCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsgI 737 CTGCAC GTGCAG(16/14) BsiEI 335 CGGCCG CG[GA][CT]^CG BsmAI 849 GTCTC GTCTC(1/5) BsmFI 1057 GGGAC GGGAC(10/14) BsoBI 309 CCCGGG C^[CT]CG[GA]G BspMI 212 ACCTGC ACCTGC(4/8) BsrDI 716 CATTGC GCAATG(2/0) BstAPI 62 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 534 GGATCT [GA]^GATC[CT] DpnII 535 GATC ^GATC DsaI 542 CCATGG C^C[GA][CT]GG EagI 335 CGGCCG C^GGCCG EclXI 335 CGGCCG C^GGCCG Eco52I 335 CGGCCG C^GGCCG MboI 535 GATC ^GATC MflI 534 GGATCT [GA]^GATC[CT] MseI 788 TTAA T^TAA NcoI 542 CCATGG C^CATGG NdeII 535 GATC ^GATC NotI 334 GCGGCCGC GC^GGCCGC PleI 1033 GACTC GAGTC(4/5) PvuII 575 CAGCTG CAG^CTG Sau3AI 535 GATC ^GATC SexAI 1010 ACCAGGT A^CC[AT]GGT SmaI 309 CCCGGG CCC^GGG SmlI 1144 CTTGAG C^T[CT][GA]AG Tru9I 788 TTAA T^TAA XcmI 969 CCACTGGGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 534 GGATCT [GA]^GATC[CT] XmaCI 309 CCCGGG C^CCGGG XmaI 309 CCCGGG C^CCGGG XmnI 613 GAAGAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### BbsI 699 GTCTTC GAAGAC(2/6) 924 GTCTTC BpuAI 699 GTCTTC GAAGAC(2/6) 924 GTCTTC EarI 384 GAAGAG CTCTTC(1/4) 795 GAAGAG EcoNI 507 CCTGGGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1143 CCTTGAGGAGG HgaI 157 GACGC GACGC(5/10) 641 GCGTC HindIII 1156 AAGCTT A^AGCTT 1189 AAGCTT Ksp632I 384 GAAGAG CTCTTC(1/4) 795 GAAGAG PflMI 126 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1011 CCAGGTCCTGG PpuMI 236 AGGACCT [GA]G^G[AT]CC[CT] 1013 AGGTCCT SapI 384 GAAGAGC GCTCTTC(1/4) 795 GAAGAGC Van91I 126 CCACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1011 CCAGGTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 78 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwNI AspI AvaII BanI BanII BbeI BbvI BcnI BfaI BmyI BpmI BsaHI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI MaeI MaeIII MboII MnlI MspA1I MspI MvaI MvnI MwoI NarI NciI NlaIII NlaIV PflFI PstI RsaI Sau96I ScrFI SfaNI SfcI SfoI StyI TfiI TseI Tsp45I Tsp509I TspRI Tth111I