(Circular) (Six-base) MAPSORT of: pFN21AE3188.seq Check: 3626 from: 1 to: 6001 pFN21AE3188 6001 bp With 182 enzymes: SgfI * March 28, 2012 14:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2226 2226 Size: 6001 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2442 3344 1937 Size: 505 902 4594 Fragments arranged by size: 4594 902 505 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2688 4064 5448 5716 1632 Size: 1056 1376 1384 268 1917 Fragments arranged by size: 1917 1384 1376 1056 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6001 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2493 2525 2493 Size: 32 5969 AlwNI CAG_nnn'CTG Cuts at: 2083 2124 2245 2416 2486 2849 2926 2976 3022 Size: 41 121 171 70 363 77 50 46 Cuts at: 3022 3744 5316 2083 Size: 722 1572 2768 Fragments arranged by size: 2768 1572 722 363 171 121 77 70 50 46 41 ApaLI G'TGCA_C Cuts at: 4058 5214 4058 Size: 1156 4845 ApoI r'AATT_y Cuts at: 1493 2622 3310 3536 3688 5797 1493 Size: 1129 688 226 152 2109 1697 Fragments arranged by size: 2109 1697 1129 688 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 2314 3326 1870 Size: 73 371 1012 4545 Fragments arranged by size: 4545 1012 371 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2153 2198 2355 3322 4777 Size: 325 217 627 366 45 157 967 1455 Cuts at: 4777 618 Size: 1842 Fragments arranged by size: 1842 1455 967 627 366 325 217 157 45 BanII G_rGCy'C Cuts at: 1391 2027 2291 2321 3320 1391 Size: 636 264 30 999 4072 Fragments arranged by size: 4072 999 636 264 30 BbeI G_GCGC'C Cuts at: 2157 2202 2359 2157 Size: 45 157 5799 Fragments arranged by size: 5799 157 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2697 2922 928 Size: 971 798 225 4007 Fragments arranged by size: 4007 971 798 225 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2564 2598 4192 4226 1903 Size: 34 627 34 1594 34 3678 Fragments arranged by size: 3678 1594 627 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2196 4612 136 Size: 107 122 71 631 605 524 2416 1525 Fragments arranged by size: 2416 1525 631 605 524 122 107 71 BglII A'GATC_T Cuts at: 5996 5996 Size: 6001 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 4062 5218 1790 Size: 2272 1156 2573 Fragments arranged by size: 2573 2272 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2956 2986 3732 Size: 416 53 83 399 24 1539 30 746 Cuts at: 3732 4690 442 Size: 958 1753 Fragments arranged by size: 1753 1539 958 746 416 399 83 53 30 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6001 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2621 2775 2621 Size: 154 5847 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2305 2317 2840 2899 4661 1239 Size: 920 146 12 523 59 1762 2579 Fragments arranged by size: 2579 1762 920 523 146 59 12 Bpu10I CC'TnA_GC Cuts at: 2409 2409 Size: 6001 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3170 4126 4991 5289 3170 Size: 956 865 298 3882 Fragments arranged by size: 3882 956 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4664 882 Size: 617 3165 2219 Fragments arranged by size: 3165 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3792 493 Size: 711 2588 2702 Fragments arranged by size: 2702 2588 711 BsaBI GATnn'nnATC Cuts at: 5995 5995 Size: 6001 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 2163 2199 2356 Size: 53 83 186 936 621 9 36 157 Cuts at: 2356 3808 4190 4825 275 Size: 1452 382 635 1451 Fragments arranged by size: 1452 1451 936 635 621 382 186 157 83 53 36 9 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 1178 Size: 30 165 30 729 30 5017 Fragments arranged by size: 5017 729 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2701 3167 3214 1186 Size: 1515 466 47 3973 Fragments arranged by size: 3973 1515 466 47 BseYI C'CCAG_C Cuts at: 2172 5204 5700 2172 Size: 3032 496 2473 Fragments arranged by size: 3032 2473 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2727 1163 Size: 573 991 4437 Fragments arranged by size: 4437 991 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2343 3451 4212 5240 1540 Size: 275 186 342 1108 761 1028 2301 Fragments arranged by size: 2301 1108 1028 761 342 275 186 BsiHKAI G_wGCw'C Cuts at: 4062 4147 5218 4062 Size: 85 1071 4845 Fragments arranged by size: 4845 1071 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2027 2291 2321 3320 4062 4147 5218 Size: 399 237 264 30 999 742 85 1071 Cuts at: 5218 1391 Size: 2174 Fragments arranged by size: 2174 1071 999 742 399 264 237 85 30 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 3356 844 Size: 1382 1130 3489 Fragments arranged by size: 3489 1382 1130 BsrBI CCG'CTC Cuts at: 1394 1823 3888 1394 Size: 429 2065 3507 Fragments arranged by size: 3507 2065 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2720 4496 4670 65 Size: 1183 1472 1776 174 1396 Fragments arranged by size: 1776 1472 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4645 4832 1700 Size: 110 2835 187 2869 Fragments arranged by size: 2869 2835 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6001 BssSI C'ACGA_G Cuts at: 4061 5073 4061 Size: 1012 4989 BstAPI GCAn_nnn'nTGC Cuts at: 794 2073 794 Size: 1279 4722 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstYI r'GATC_y Cuts at: 1972 2539 3331 3698 4083 4100 5552 5638 5650 Size: 567 792 367 385 17 1452 86 12 Cuts at: 5650 5996 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 792 567 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2547 513 Size: 552 939 543 3967 Fragments arranged by size: 3967 939 552 543 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2131 2302 3517 4311 4331 2131 Size: 171 1215 794 20 3801 Fragments arranged by size: 3801 1215 794 171 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2113 2247 Size: 54 1425 50 275 18 216 67 134 Cuts at: 2247 2340 3448 4337 5966 8 Size: 93 1108 889 1629 43 Fragments arranged by size: 1629 1425 1108 889 275 216 134 93 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2340 3448 1537 Size: 275 528 1108 4090 Fragments arranged by size: 4090 1108 528 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2384 2795 3931 1353 Size: 1031 411 1136 3423 Fragments arranged by size: 3423 1136 1031 411 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2017 4588 4962 5108 387 Size: 1064 138 428 2571 374 146 1280 Fragments arranged by size: 2571 1280 1064 428 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2305 2317 2688 2840 2899 4064 Size: 393 527 146 12 371 152 59 1165 Cuts at: 4064 4661 5448 5716 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1165 787 597 527 393 371 268 152 146 59 12 EcoNI CCTnn'n_nnAGG Cuts at: 2516 3152 2516 Size: 636 5365 EcoO109I rG'GnC_Cy Cuts at: 2143 2242 2349 2472 2923 3019 2143 Size: 99 107 123 451 96 5125 Fragments arranged by size: 5125 451 123 107 99 96 EcoRI G'AATT_C Cuts at: 3310 3688 3310 Size: 378 5623 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3184 3184 Size: 6001 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 2157 2202 2359 5148 1678 Size: 479 45 157 2789 2531 Fragments arranged by size: 2789 2531 479 157 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2839 2871 4642 4674 4716 Size: 32 135 32 1267 32 1771 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 1771 1267 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 3161 3194 748 Size: 2413 33 3555 Fragments arranged by size: 3555 2413 33 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2153 2198 2355 2153 Size: 45 157 5799 Fragments arranged by size: 5799 157 45 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2316 2598 2810 5115 5299 1305 Size: 1011 282 212 2305 184 2007 Fragments arranged by size: 2305 2007 1011 282 212 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4179 Fragments arranged by size: 4179 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3959 4318 4477 518 Size: 648 2793 359 159 2042 Fragments arranged by size: 2793 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2172 2374 2582 3417 4094 5242 5487 1606 Size: 566 202 208 835 677 1148 245 2120 Fragments arranged by size: 2120 1148 835 677 566 245 208 202 NarI GG'CG_CC Cuts at: 2154 2199 2356 2154 Size: 45 157 5799 Fragments arranged by size: 5799 157 45 NcoI C'CATG_G Cuts at: 513 1065 2004 2547 513 Size: 552 939 543 3967 Fragments arranged by size: 3967 939 552 543 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6001 NotI GC'GGCC_GC Cuts at: 2340 3448 2340 Size: 1108 4893 NspI r_CATG'y Cuts at: 1132 1229 3359 4904 1132 Size: 97 2130 1545 2229 Fragments arranged by size: 2229 2130 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 2137 3022 1108 Size: 1029 885 4087 Fragments arranged by size: 4087 1029 885 PfoI T'CCnGG_A Cuts at: 2691 3265 2691 Size: 574 5427 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 3019 2242 Size: 777 5224 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PstI C_TGCA'G Cuts at: 830 2241 2373 2463 2587 2816 3353 830 Size: 1411 132 90 124 229 537 3478 Fragments arranged by size: 3478 1411 537 229 132 124 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2582 2582 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SapI GCTCTTCn'nnn_ Cuts at: 2384 2795 2384 Size: 411 5590 SbfI CC_TGCA'GG Cuts at: 3353 3353 Size: 6001 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SexAI A'CCwGG_T Cuts at: 3015 3015 Size: 6001 SfcI C'TryA_G Cuts at: 826 1045 2237 2369 2459 2583 2812 3349 4484 Size: 219 1192 132 90 124 229 537 1135 Cuts at: 4484 5165 5356 826 Size: 681 191 1471 Fragments arranged by size: 1471 1192 1135 681 537 229 219 191 132 124 90 SfoI GGC'GCC Cuts at: 2155 2200 2357 2155 Size: 45 157 5799 Fragments arranged by size: 5799 157 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 2316 3328 1872 Size: 444 1012 4545 Fragments arranged by size: 4545 1012 444 SmlI C'TyrA_G Cuts at: 820 1017 1943 3149 4105 5006 5268 820 Size: 197 926 1206 956 901 262 1553 Fragments arranged by size: 1553 1206 956 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 3359 1132 Size: 2227 3774 SrfI GCCC'GGGC Cuts at: 2316 2316 Size: 6001 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2004 2547 2829 3106 3440 513 Size: 552 939 543 282 277 334 3074 Fragments arranged by size: 3074 939 552 543 334 282 277 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4028 4045 4198 4383 4828 325 Size: 1255 2448 17 153 185 445 1498 Fragments arranged by size: 2448 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4247 5959 96 Size: 274 80 33 51 1443 2270 1712 138 Fragments arranged by size: 2270 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2132 2164 5961 5993 2132 Size: 32 3797 32 2140 Fragments arranged by size: 3797 2140 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2447 2851 3017 1316 Size: 1131 404 166 4300 Fragments arranged by size: 4300 1131 404 166 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2981 2011 Size: 970 5031 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 2314 3326 1870 Size: 444 1012 4545 Fragments arranged by size: 4545 1012 444 XmnI GAAnn'nnTTC Cuts at: 2622 4130 2622 Size: 1508 4493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SwaI