(Linear) (Six-base) MAPSORT of: KIEE3188.seq Check: 8321 from: 1 to: 1296 KIEE3188.orf With 182 enzymes: SgfI * March 22, 2012 16:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 221 1296 Size: 221 1075 AccI GT'mk_AC Cuts at: 0 437 1296 Size: 437 859 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 683 1296 Size: 683 613 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 488 520 1296 Size: 488 32 776 Fragments arranged by size: 776 488 32 AlwNI CAG_nnn'CTG Cuts at: 0 78 119 240 411 481 844 921 971 Size: 78 41 121 171 70 363 77 50 Cuts at: 971 1017 1296 Size: 46 279 Fragments arranged by size: 363 279 171 121 78 77 70 50 46 41 ApoI r'AATT_y Cuts at: 0 617 1296 Size: 617 679 AvaI C'yCGr_G Cuts at: 0 309 1296 Size: 309 987 BanI G'GyrC_C Cuts at: 0 148 193 350 1296 Size: 148 45 157 946 Fragments arranged by size: 946 157 148 45 BanII G_rGCy'C Cuts at: 0 22 286 316 1296 Size: 22 264 30 980 Fragments arranged by size: 980 264 30 22 BbeI G_GCGC'C Cuts at: 0 152 197 354 1296 Size: 152 45 157 942 Fragments arranged by size: 942 157 152 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 692 917 1296 Size: 692 225 379 Fragments arranged by size: 692 379 225 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 559 593 1296 Size: 559 34 703 Fragments arranged by size: 703 559 34 BglI GCCn_nnn'nGGC Cuts at: 0 191 1296 Size: 191 1105 BmrI ACTGGGnnnn_n' Cuts at: 0 951 981 1296 Size: 951 30 315 Fragments arranged by size: 951 315 30 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 616 770 1296 Size: 616 154 526 Fragments arranged by size: 616 526 154 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 300 312 835 894 1296 Size: 154 146 12 523 59 402 Fragments arranged by size: 523 402 154 146 59 12 Bpu10I CC'TnA_GC Cuts at: 0 404 1296 Size: 404 892 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1165 1296 Size: 1165 131 BsaHI Gr'CG_yC Cuts at: 0 149 158 194 351 1296 Size: 149 9 36 157 945 Fragments arranged by size: 945 157 149 36 9 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 127 157 1296 Size: 127 30 1139 Fragments arranged by size: 1139 127 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 696 1162 1209 1296 Size: 696 466 47 87 Fragments arranged by size: 696 466 87 47 BseYI C'CCAG_C Cuts at: 0 167 1296 Size: 167 1129 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 722 1296 Size: 722 574 BsiEI CG_ry'CG Cuts at: 0 338 1296 Size: 338 958 Bsp1286I G_dGCh'C Cuts at: 0 22 286 316 1296 Size: 22 264 30 980 Fragments arranged by size: 980 264 30 22 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 221 1296 Size: 221 1075 BsrDI GCAATG_nn' Cuts at: 0 715 1296 Size: 715 581 BstAPI GCAn_nnn'nTGC Cuts at: 0 68 1296 Size: 68 1228 BstYI r'GATC_y Cuts at: 0 534 1296 Size: 534 762 BtgI C'CryG_G Cuts at: 0 542 1296 Size: 542 754 BtsI GCAGTG_nn' Cuts at: 0 126 297 1296 Size: 126 171 999 Fragments arranged by size: 999 171 126 EaeI y'GGCC_r Cuts at: 0 41 108 242 335 1296 Size: 41 67 134 93 961 Fragments arranged by size: 961 134 93 67 41 EagI C'GGCC_G Cuts at: 0 335 1296 Size: 335 961 EarI CTCTTCn'nnn_ Cuts at: 0 379 790 1296 Size: 379 411 506 Fragments arranged by size: 506 411 379 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 12 1296 Size: 12 1284 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 154 300 312 683 835 894 1296 Size: 154 146 12 371 152 59 402 Fragments arranged by size: 402 371 154 152 146 59 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 511 1147 1296 Size: 511 636 149 Fragments arranged by size: 636 511 149 EcoO109I rG'GnC_Cy Cuts at: 0 138 237 344 467 918 1014 1296 Size: 138 99 107 123 451 96 282 Fragments arranged by size: 451 282 138 123 107 99 96 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1179 1296 Size: 1179 117 HaeII r_GCGC'y Cuts at: 0 152 197 354 1296 Size: 152 45 157 942 Fragments arranged by size: 942 157 152 45 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 834 866 1296 Size: 834 32 430 Fragments arranged by size: 834 430 32 HindIII A'AGCT_T Cuts at: 0 1156 1189 1296 Size: 1156 33 107 Fragments arranged by size: 1156 107 33 KasI G'GCGC_C Cuts at: 0 148 193 350 1296 Size: 148 45 157 946 Fragments arranged by size: 946 157 148 45 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 311 593 805 1296 Size: 311 282 212 491 Fragments arranged by size: 491 311 282 212 MspA1I CmG'CkG Cuts at: 0 167 369 577 1296 Size: 167 202 208 719 Fragments arranged by size: 719 208 202 167 NarI GG'CG_CC Cuts at: 0 149 194 351 1296 Size: 149 45 157 945 Fragments arranged by size: 945 157 149 45 NcoI C'CATG_G Cuts at: 0 542 1296 Size: 542 754 NotI GC'GGCC_GC Cuts at: 0 335 1296 Size: 335 961 PflMI CCAn_nnn'nTGG Cuts at: 0 132 1017 1296 Size: 132 885 279 Fragments arranged by size: 885 279 132 PfoI T'CCnGG_A Cuts at: 0 686 1260 1296 Size: 686 574 36 Fragments arranged by size: 686 574 36 PpuMI rG'GwC_Cy Cuts at: 0 237 1014 1296 Size: 237 777 282 Fragments arranged by size: 777 282 237 PstI C_TGCA'G Cuts at: 0 236 368 458 582 811 1296 Size: 236 132 90 124 229 485 Fragments arranged by size: 485 236 229 132 124 90 PvuII CAG'CTG Cuts at: 0 577 1296 Size: 577 719 SapI GCTCTTCn'nnn_ Cuts at: 0 379 790 1296 Size: 379 411 506 Fragments arranged by size: 506 411 379 SexAI A'CCwGG_T Cuts at: 0 1010 1296 Size: 1010 286 SfcI C'TryA_G Cuts at: 0 232 364 454 578 807 1296 Size: 232 132 90 124 229 489 Fragments arranged by size: 489 232 229 132 124 90 SfoI GGC'GCC Cuts at: 0 150 195 352 1296 Size: 150 45 157 944 Fragments arranged by size: 944 157 150 45 SmaI CCC'GGG Cuts at: 0 311 1296 Size: 311 985 SmlI C'TyrA_G Cuts at: 0 1144 1296 Size: 1144 152 SrfI GCCC'GGGC Cuts at: 0 311 1296 Size: 311 985 StyI C'CwwG_G Cuts at: 0 542 824 1101 1296 Size: 542 282 277 195 Fragments arranged by size: 542 282 277 195 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 127 159 1296 Size: 127 32 1137 Fragments arranged by size: 1137 127 32 Tth111I GACn'n_nGTC Cuts at: 0 442 846 1012 1296 Size: 442 404 166 284 Fragments arranged by size: 442 404 284 166 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 976 1296 Size: 976 320 XmaI C'CCGG_G Cuts at: 0 309 1296 Size: 309 987 XmnI GAAnn'nnTTC Cuts at: 0 617 1296 Size: 617 679 Enzymes that do cut: AarI AccI AcuI AloI AlwNI ApoI AvaI BanI BanII BbeI BbsI BcgI BglI BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BspMI BsrDI BstAPI BstYI BtgI BtsI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KasI MmeI MspA1I NarI NcoI NotI PflMI PfoI PpuMI PstI PvuII SapI SexAI SfcI SfoI SmaI SmlI SrfI StyI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BsaI BsaAI BsaBI BsaWI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HpaI KpnI MfeI MluI MscI MslI NaeI NdeI NgoMIV NheI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SspI StuI SwaI TaqII TatI XbaI XhoI ZraI