################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AfaI AflIII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbrPI BciVI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI Hin1I HincII HindII HpaI KasI KpnI KspI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I RcaI RsaI RsrII SacII SalI SbfI ScaI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 881 TCCGGA T^CCGGA AflII 247 CTTAAG C^TTAAG ApaI 966 GGGCCC GGGCC^C BbvCI 431 CCTCAGC CCTCAGC(-5/-2) BclI 1646 TGATCA T^GATCA BfrI 247 CTTAAG C^TTAAG BsaBI 207 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 881 TCCGGA [AT]^CCGG[AT] BseAI 881 TCCGGA T^CCGGA BsiEI 1309 CGATCG CG[GA][CT]^CG BspEI 881 TCCGGA T^CCGGA BsrDI 1374 CATTGC GCAATG(2/0) BstEII 561 GGTGACC G^GT[ACGT]ACC BstPI 561 GGTGACC G^GT[ACGT]ACC BstYI 857 AGATCC [GA]^GATC[CT] EcoNI 1290 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 561 GGTGACC G^GT[ACGT]ACC FbaI 1646 TGATCA T^GATCA HaeII 135 GGCGCT [GA]GCGC^[CT] HgaI 938 GCGTC GACGC(5/10) HindIII 568 AAGCTT A^AGCTT MamI 207 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 857 AGATCC [GA]^GATC[CT] MroI 881 TCCGGA T^CCGGA NspI 456 ACATGC [GA]CATG^[CT] PflMI 5 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuI 1309 CGATCG CGAT^CG SacI 532 GAGCTC GAGCT^C SexAI 728 ACCTGGT A^CC[AT]GGT SfiI 18 GGCCCTGTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 247 CTTAAG C^T[CT][GA]AG StuI 104 AGGCCT AGG^CCT TfiI 1619 GATTC G^A[AT]TC Van91I 5 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 122 CCATCATCCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 857 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 39 enzymes) # ################################### AccII 114 CGCG CG^CG 294 CGCG Alw44I 147 GTGCAC G^TGCAC 1416 GTGCAC AlwI 858 GATCC GGATC(4/5) 413 GGATC AlwNI 16 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1735 CAGTGCCTG ApaLI 147 GTGCAC G^TGCAC 1416 GTGCAC AvaI 1034 CCCGGG C^[CT]CG[GA]G 1610 CCCGGG BanI 444 GGTGCC G^G[CT][GA]CC 715 GGCACC BanII 532 GAGCTC G[GA]GC[CT]^C 966 GGGCCC BbsI 1230 GAAGAC GAAGAC(2/6) 1573 GTCTTC BfaI 1421 CTAG C^TAG 1459 CTAG BglI 19 GCCCTGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1462 GCCTCCATGGC BpmI 535 CTCCAG CTGGAG(16/14) 1367 CTCCAG BpuAI 1230 GAAGAC GAAGAC(2/6) 1573 GTCTTC BsoBI 1034 CCCGGG C^[CT]CG[GA]G 1610 CCCGGG BspMI 7 ACCTGC ACCTGC(4/8) 311 ACCTGC BstAPI 954 GCACAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1353 GCAGAGTGTGC BstUI 114 CGCG CG^CG 294 CGCG BstXI 290 CCATCGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1523 CCACCTCCCTGG CfoI 113 GCGC GCG^C 136 GCGC DraIII 651 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG 1410 CACCGAGTG FauI 366 CCCGC CCCGC(4/6) 1557 CCCGC HhaI 113 GCGC GCG^C 136 GCGC HinP1I 113 GCGC G^CGC 136 GCGC MaeI 1421 CTAG C^TAG 1459 CTAG MseI 190 TTAA T^TAA 248 TTAA MspA1I 472 CAGCTG C[AC]G^C[GT]G 1668 CAGCTG MvnI 114 CGCG CG^CG 294 CGCG NcoI 818 CCATGG C^CATGG 1466 CCATGG PleI 253 GACTC GAGTC(4/5) 1640 GAGTC PpuMI 1178 AGGACCC [GA]G^G[AT]CC[CT] 1586 GGGACCT PstI 853 CTGCAG CTGCA^G 1351 CTGCAG PvuII 472 CAGCTG CAG^CTG 1668 CAGCTG SapI 138 GCTCTTC GCTCTTC(1/4) 756 GCTCTTC SfcI 853 CTGCAG C^T[GA][CT]AG 1351 CTGCAG SmaI 1034 CCCGGG CCC^GGG 1610 CCCGGG Tru9I 190 TTAA T^TAA 248 TTAA Tsp509I 1066 AATT ^AATT 1137 AATT XmaCI 1034 CCCGGG C^CCGGG 1610 CCCGGG XmaI 1034 CCCGGG C^CCGGG 1610 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AluI AspHI AvaII BalI BbvI BcgI BcnI BmyI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp45I TspRI TthHB8I