(Circular) (Six-base) MAPSORT of: pFN21AB6562.seq Check: 2338 from: 1 to: 5902 pFN21AB6562 5902 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3712 278 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2549 3245 1937 Size: 612 696 4594 Fragments arranged by size: 4594 696 612 Acc65I G'GTAC_C Cuts at: 3223 3223 Size: 5902 AclI AA'CG_TT Cuts at: 1563 4029 4402 1563 Size: 2466 373 3063 Fragments arranged by size: 3063 2466 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2156 2285 2494 3965 5349 5617 1632 Size: 524 129 209 1471 1384 268 1917 Fragments arranged by size: 1917 1471 1384 524 268 209 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5705 AflIII A'CryG_T Cuts at: 1203 4801 5778 1203 Size: 3598 977 1327 Fragments arranged by size: 3598 1327 977 AgeI A'CCGG_T Cuts at: 4733 4733 Size: 5902 AhdI GACnn_n'nnGTC Cuts at: 4631 4631 Size: 5902 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5902 AlwNI CAG_nnn'CTG Cuts at: 3645 5217 3645 Size: 1572 4330 ApaLI G'TGCA_C Cuts at: 2502 3959 5115 2502 Size: 1457 1156 3289 Fragments arranged by size: 3289 1457 1156 ApoI r'AATT_y Cuts at: 1493 2143 2400 2613 3008 3211 3437 3589 5698 Size: 650 257 213 395 203 226 152 2109 Cuts at: 5698 1493 Size: 1697 Fragments arranged by size: 2109 1697 650 395 257 226 213 203 152 AseI AT'TA_AT Cuts at: 160 1032 4456 160 Size: 872 3424 1606 Fragments arranged by size: 3424 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 AvaI C'yCGr_G Cuts at: 1870 1943 2020 3016 3227 1870 Size: 73 77 996 211 4545 Fragments arranged by size: 4545 996 211 77 73 BamHI G'GATC_C Cuts at: 3232 3599 3232 Size: 367 5535 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2060 2086 2815 3223 4678 Size: 325 217 627 273 26 729 408 1455 Cuts at: 4678 618 Size: 1842 Fragments arranged by size: 1842 1455 729 627 408 325 273 217 26 BanII G_rGCy'C Cuts at: 1391 2021 3221 1391 Size: 630 1200 4072 Fragments arranged by size: 4072 1200 630 BbeI G_GCGC'C Cuts at: 2090 2090 Size: 5902 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4931 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4093 4127 1903 Size: 34 2156 34 3678 Fragments arranged by size: 3678 2156 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3794 5004 3794 Size: 1210 4692 BclI T'GATC_A Cuts at: 1548 2947 1548 Size: 1399 4503 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2025 4513 Size: 107 122 71 631 605 337 16 2488 Cuts at: 4513 136 Size: 1525 Fragments arranged by size: 2488 1525 631 605 337 122 107 71 16 BglII A'GATC_T Cuts at: 2268 5897 2268 Size: 3629 2273 BlpI GC'TnA_GC Cuts at: 3319 3319 Size: 5902 Bme1580I G_kGCm'C Cuts at: 1790 2506 3963 5119 1790 Size: 716 1457 1156 2573 Fragments arranged by size: 2573 1457 1156 716 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5902 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2296 3633 4591 Size: 416 53 83 399 24 879 1337 958 Cuts at: 4591 442 Size: 1753 Fragments arranged by size: 1753 1337 958 879 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5902 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2896 4562 1239 Size: 1657 1666 2579 Fragments arranged by size: 2579 1666 1657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4027 4892 5190 4027 Size: 865 298 4739 Fragments arranged by size: 4739 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4565 882 Size: 617 3066 2219 Fragments arranged by size: 3066 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3693 493 Size: 711 2489 2702 Fragments arranged by size: 2702 2489 711 BsaBI GATnn'nnATC Cuts at: 5896 5896 Size: 5902 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2087 3709 4091 4726 Size: 53 83 186 936 554 1622 382 635 Cuts at: 4726 275 Size: 1451 Fragments arranged by size: 1622 1451 936 635 554 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4335 4733 5007 5154 5565 4335 Size: 398 274 147 411 4672 Fragments arranged by size: 4672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5677 Fragments arranged by size: 5677 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2349 1186 Size: 1163 4739 BseYI C'CCAG_C Cuts at: 3179 5105 5601 3179 Size: 1926 496 3480 Fragments arranged by size: 3480 1926 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5329 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2103 3352 4113 5141 1540 Size: 275 186 102 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 275 186 102 BsiHKAI G_wGCw'C Cuts at: 2506 3963 4048 5119 2506 Size: 1457 85 1071 3289 Fragments arranged by size: 3289 1457 1071 85 BsmI GAATG_Cn' Cuts at: 3413 3506 3413 Size: 93 5809 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2506 3221 3963 4048 5119 1391 Size: 399 231 485 715 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 715 485 399 231 85 BspEI T'CCGG_A Cuts at: 5565 5565 Size: 5902 BspHI T'CATG_A Cuts at: 3791 5521 3791 Size: 1730 4172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3257 844 Size: 2413 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2025 2865 3789 1394 Size: 429 202 840 924 3507 Fragments arranged by size: 3507 924 840 429 202 BsrDI GCAATG_nn' Cuts at: 65 1248 4397 4571 65 Size: 1183 3149 174 1396 Fragments arranged by size: 3149 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4546 4733 1700 Size: 110 2736 187 2869 Fragments arranged by size: 2869 2736 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5902 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5902 BssSI C'ACGA_G Cuts at: 3962 4974 3962 Size: 1012 4890 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5902 BstBI TT'CG_AA Cuts at: 4706 4706 Size: 5902 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5902 BstYI r'GATC_y Cuts at: 1972 2268 2529 2974 3232 3599 3984 4001 5453 Size: 296 261 445 258 367 385 17 1452 Cuts at: 5453 5539 5551 5897 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 445 385 367 346 296 261 258 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4411 Fragments arranged by size: 4411 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5163 Fragments arranged by size: 5163 683 56 BtsI GCAGTG_nn' Cuts at: 2295 3418 4212 4232 2295 Size: 1123 794 20 3965 Fragments arranged by size: 3965 1123 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5902 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2356 2391 529 Size: 35 1792 35 4040 Fragments arranged by size: 4040 1792 35 35 DraI TTT'AAA Cuts at: 3206 3206 Size: 5902 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4909 809 Size: 507 3593 1802 Fragments arranged by size: 3593 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2904 3349 Size: 54 1425 50 275 18 270 804 445 Cuts at: 3349 4238 5867 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 804 445 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2100 3349 1537 Size: 275 288 1249 4090 Fragments arranged by size: 4090 1249 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2513 3832 1353 Size: 1160 1319 3423 Fragments arranged by size: 3423 1319 1160 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4489 4863 5009 387 Size: 1064 138 2900 374 146 1280 Fragments arranged by size: 2900 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2285 2494 2896 3965 4562 5349 Size: 393 524 129 209 402 1069 597 787 Cuts at: 5349 5617 1239 Size: 268 1524 Fragments arranged by size: 1524 1069 787 597 524 402 393 268 209 129 EcoNI CCTnn'n_nnAGG Cuts at: 3050 3050 Size: 5902 EcoO109I rG'GnC_Cy Cuts at: 2079 2079 Size: 5902 EcoRI G'AATT_C Cuts at: 2400 3008 3211 3589 2400 Size: 608 203 378 4713 Fragments arranged by size: 4713 608 378 203 EcoRV GAT'ATC Cuts at: 2692 2692 Size: 5902 FspI TGC'GCA Cuts at: 4408 4408 Size: 5902 HaeII r_GCGC'y Cuts at: 1678 2090 5049 1678 Size: 412 2959 2531 Fragments arranged by size: 2959 2531 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2844 2849 2876 2881 4543 Size: 32 135 32 1272 5 27 5 1662 Cuts at: 4543 4575 4617 4649 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1662 1272 135 42 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 2353 2409 3037 3246 3492 669 Size: 988 281 415 56 628 209 246 3079 Fragments arranged by size: 3079 988 628 415 281 246 209 56 HindIII A'AGCT_T Cuts at: 748 748 Size: 5902 HpaI GTT'AAC Cuts at: 3492 3492 Size: 5902 KasI G'GCGC_C Cuts at: 2086 2086 Size: 5902 KpnI G_GTAC'C Cuts at: 3227 3227 Size: 5902 MfeI C'AATT_G Cuts at: 3501 3501 Size: 5902 MluI A'CGCG_T Cuts at: 5778 5778 Size: 5902 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2507 5016 5200 1305 Size: 1202 2509 184 2007 Fragments arranged by size: 2509 2007 1202 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2906 10 Size: 54 1425 343 1074 3006 Fragments arranged by size: 3006 1425 1074 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3860 4219 4378 518 Size: 648 2694 359 159 2042 Fragments arranged by size: 2694 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2052 2058 2986 3318 3995 5143 5388 1606 Size: 446 6 928 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 928 677 446 332 245 6 NarI GG'CG_CC Cuts at: 2087 2087 Size: 5902 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4411 Fragments arranged by size: 4411 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5902 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5902 NotI GC'GGCC_GC Cuts at: 3349 3349 Size: 5902 NspI r_CATG'y Cuts at: 1132 1229 2116 3141 3260 4805 1132 Size: 97 887 1025 119 1545 2229 Fragments arranged by size: 2229 1545 1025 887 119 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5902 PciI A'CATG_T Cuts at: 4801 4801 Size: 5902 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5902 PfoI T'CCnGG_A Cuts at: 3156 3156 Size: 5902 PmeI GTTT'AAAC Cuts at: 3206 3206 Size: 5902 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3971 4003 5484 5516 3971 Size: 32 1481 32 4357 Fragments arranged by size: 4357 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2079 2079 Size: 5902 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5902 PsiI TTA'TAA Cuts at: 3472 3472 Size: 5902 PstI C_TGCA'G Cuts at: 830 2302 3254 830 Size: 1472 952 3478 Fragments arranged by size: 3478 1472 952 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5902 PvuII CAG'CTG Cuts at: 2986 2986 Size: 5902 SalI G'TCGA_C Cuts at: 1936 3244 1936 Size: 1308 4594 SanDI GG'GwC_CC Cuts at: 2079 2079 Size: 5902 SbfI CC_TGCA'GG Cuts at: 3254 3254 Size: 5902 ScaI AGT'ACT Cuts at: 4150 5862 4150 Size: 1712 4190 SfcI C'TryA_G Cuts at: 826 1045 2169 2199 2298 2800 3250 4385 5066 Size: 219 1124 30 99 502 450 1135 681 Cuts at: 5066 5257 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1124 681 502 450 219 191 99 30 SfoI GGC'GCC Cuts at: 2088 2088 Size: 5902 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5902 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5902 SmaI CCC'GGG Cuts at: 1872 3229 1872 Size: 1357 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4006 4907 5169 820 Size: 197 926 2063 901 262 1553 Fragments arranged by size: 2063 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3693 493 Size: 3200 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5902 SphI G_CATG'C Cuts at: 1132 2116 3141 3260 1132 Size: 984 1025 119 3774 Fragments arranged by size: 3774 1025 984 119 SspI AAT'ATT Cuts at: 5 52 2707 2731 3826 5 Size: 47 2655 24 1095 2081 Fragments arranged by size: 2655 2081 1095 47 24 StuI AGG'CCT Cuts at: 1517 1517 Size: 5902 StyI C'CwwG_G Cuts at: 513 1065 2004 2317 3341 513 Size: 552 939 313 1024 3074 Fragments arranged by size: 3074 1024 939 552 313 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2394 3929 3946 4099 4284 4729 325 Size: 1255 814 1535 17 153 185 445 1498 Fragments arranged by size: 1535 1498 1255 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4148 5860 96 Size: 274 80 33 51 1443 2171 1712 138 Fragments arranged by size: 2171 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2751 2783 5862 5894 2751 Size: 32 3079 32 2759 Fragments arranged by size: 3079 2759 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5902 XbaI T'CTAG_A Cuts at: 3238 3238 Size: 5902 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2372 2710 2372 Size: 338 5564 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5902 XmaI C'CCGG_G Cuts at: 1870 3227 1870 Size: 1357 4545 XmnI GAAnn'nnTTC Cuts at: 4031 4031 Size: 5902 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3710 276 Size: 53 83 186 936 2176 2468 Fragments arranged by size: 2468 2176 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI