Restriction map for pFN21AB6562
 (Circular) (Six-base) MAPSORT of: pFN21AB6562.seq  Check: 2338  from: 1  to: 5902



pFN21AB6562  5902 bp

 With 182 enzymes: SgfI * 

                        October 25, 2019 12:32  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3712     278 
   Size:         53      83     186     936    2176    2468

  Fragments arranged by size: 

                2468    2176     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2549    3245    1937 
   Size:        612     696    4594

  Fragments arranged by size: 

                4594     696     612
 
Acc65I G'GTAC_C

Cuts at:   3223    3223 
   Size:       5902
 
AclI AA'CG_TT

Cuts at:   1563    4029    4402    1563 
   Size:       2466     373    3063

  Fragments arranged by size: 

                3063    2466     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2156    2285    2494    3965    5349    5617    1632 
   Size:        524     129     209    1471    1384     268    1917

  Fragments arranged by size: 

                1917    1471    1384     524     268     209     129
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    5705
 
AflIII A'CryG_T

Cuts at:   1203    4801    5778    1203 
   Size:       3598     977    1327

  Fragments arranged by size: 

                3598    1327     977
 
AgeI A'CCGG_T

Cuts at:   4733    4733 
   Size:       5902
 
AhdI GACnn_n'nnGTC

Cuts at:   4631    4631 
   Size:       5902
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5902
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5902
 
AlwNI CAG_nnn'CTG

Cuts at:   3645    5217    3645 
   Size:       1572    4330
 
ApaLI G'TGCA_C

Cuts at:   2502    3959    5115    2502 
   Size:       1457    1156    3289

  Fragments arranged by size: 

                3289    1457    1156
 
ApoI r'AATT_y

Cuts at:   1493    2143    2400    2613    3008    3211    3437    3589    5698 
   Size:        650     257     213     395     203     226     152    2109

Cuts at:   5698    1493 
   Size:       1697

  Fragments arranged by size: 

                2109    1697     650     395     257     226     213     203
                 152
 
AseI AT'TA_AT

Cuts at:    160    1032    4456     160 
   Size:        872    3424    1606

  Fragments arranged by size: 

                3424    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5902
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2020    3016    3227    1870 
   Size:         73      77     996     211    4545

  Fragments arranged by size: 

                4545     996     211      77      73
 
BamHI G'GATC_C

Cuts at:   3232    3599    3232 
   Size:        367    5535
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2060    2086    2815    3223    4678 
   Size:        325     217     627     273      26     729     408    1455

Cuts at:   4678     618 
   Size:       1842

  Fragments arranged by size: 

                1842    1455     729     627     408     325     273     217
                  26
 
BanII G_rGCy'C

Cuts at:   1391    2021    3221    1391 
   Size:        630    1200    4072

  Fragments arranged by size: 

                4072    1200     630
 
BbeI G_GCGC'C

Cuts at:   2090    2090 
   Size:       5902
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4931
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    4093    4127    1903 
   Size:         34    2156      34    3678

  Fragments arranged by size: 

                3678    2156      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3794    5004    3794 
   Size:       1210    4692
 
BclI T'GATC_A

Cuts at:   1548    2947    1548 
   Size:       1399    4503
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2009    2025    4513 
   Size:        107     122      71     631     605     337      16    2488

Cuts at:   4513     136 
   Size:       1525

  Fragments arranged by size: 

                2488    1525     631     605     337     122     107      71
                  16
 
BglII A'GATC_T

Cuts at:   2268    5897    2268 
   Size:       3629    2273
 
BlpI GC'TnA_GC

Cuts at:   3319    3319 
   Size:       5902
 
Bme1580I G_kGCm'C

Cuts at:   1790    2506    3963    5119    1790 
   Size:        716    1457    1156    2573

  Fragments arranged by size: 

                2573    1457    1156     716
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5902
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2296    3633    4591 
   Size:        416      53      83     399      24     879    1337     958

Cuts at:   4591     442 
   Size:       1753

  Fragments arranged by size: 

                1753    1337     958     879     416     399      83      53
                  24
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5902
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2896    4562    1239 
   Size:       1657    1666    2579

  Fragments arranged by size: 

                2579    1666    1657
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   4027    4892    5190    4027 
   Size:        865     298    4739

  Fragments arranged by size: 

                4739     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4565     882 
   Size:        617    3066    2219

  Fragments arranged by size: 

                3066    2219     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3693     493 
   Size:        711    2489    2702

  Fragments arranged by size: 

                2702    2489     711
 
BsaBI GATnn'nnATC

Cuts at:   5896    5896 
   Size:       5902
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2087    3709    4091    4726 
   Size:         53      83     186     936     554    1622     382     635

Cuts at:   4726     275 
   Size:       1451

  Fragments arranged by size: 

                1622    1451     936     635     554     382     186      83
                  53
 
BsaWI w'CCGG_w

Cuts at:   4335    4733    5007    5154    5565    4335 
   Size:        398     274     147     411    4672

  Fragments arranged by size: 

                4672     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5677

  Fragments arranged by size: 

                5677     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2349    1186 
   Size:       1163    4739
 
BseYI C'CCAG_C

Cuts at:   3179    5105    5601    3179 
   Size:       1926     496    3480

  Fragments arranged by size: 

                3480    1926     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    5329
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2103    3352    4113    5141    1540 
   Size:        275     186     102    1249     761    1028    2301

  Fragments arranged by size: 

                2301    1249    1028     761     275     186     102
 
BsiHKAI G_wGCw'C

Cuts at:   2506    3963    4048    5119    2506 
   Size:       1457      85    1071    3289

  Fragments arranged by size: 

                3289    1457    1071      85
 
BsmI GAATG_Cn'

Cuts at:   3413    3506    3413 
   Size:         93    5809
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2021    2506    3221    3963    4048    5119    1391 
   Size:        399     231     485     715     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     715     485     399     231      85
            
 
BspEI T'CCGG_A

Cuts at:   5565    5565 
   Size:       5902
 
BspHI T'CATG_A

Cuts at:   3791    5521    3791 
   Size:       1730    4172
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    3257     844 
   Size:       2413    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2025    2865    3789    1394 
   Size:        429     202     840     924    3507

  Fragments arranged by size: 

                3507     924     840     429     202
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    4397    4571      65 
   Size:       1183    3149     174    1396

  Fragments arranged by size: 

                3149    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4546    4733    1700 
   Size:        110    2736     187    2869

  Fragments arranged by size: 

                2869    2736     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5902
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5902
 
BssSI C'ACGA_G

Cuts at:   3962    4974    3962 
   Size:       1012    4890
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5902
 
BstBI TT'CG_AA

Cuts at:   4706    4706 
   Size:       5902
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5902
 
BstYI r'GATC_y

Cuts at:   1972    2268    2529    2974    3232    3599    3984    4001    5453 
   Size:        296     261     445     258     367     385      17    1452

Cuts at:   5453    5539    5551    5897    1972 
   Size:         86      12     346    1977

  Fragments arranged by size: 

                1977    1452     445     385     367     346     296     261
                 258      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4411

  Fragments arranged by size: 

                4411     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    5163

  Fragments arranged by size: 

                5163     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2295    3418    4212    4232    2295 
   Size:       1123     794      20    3965

  Fragments arranged by size: 

                3965    1123     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5902
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2356    2391     529 
   Size:         35    1792      35    4040

  Fragments arranged by size: 

                4040    1792      35      35
 
DraI TTT'AAA

Cuts at:   3206    3206 
   Size:       5902
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4909     809 
   Size:        507    3593    1802

  Fragments arranged by size: 

                3593    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2100    2904    3349 
   Size:         54    1425      50     275      18     270     804     445

Cuts at:   3349    4238    5867       8 
   Size:        889    1629      43

  Fragments arranged by size: 

                1629    1425     889     804     445     275     270      54
                  50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2100    3349    1537 
   Size:        275     288    1249    4090

  Fragments arranged by size: 

                4090    1249     288     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2513    3832    1353 
   Size:       1160    1319    3423

  Fragments arranged by size: 

                3423    1319    1160
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4489    4863    5009     387 
   Size:       1064     138    2900     374     146    1280

  Fragments arranged by size: 

                2900    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2156    2285    2494    2896    3965    4562    5349 
   Size:        393     524     129     209     402    1069     597     787

Cuts at:   5349    5617    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1524    1069     787     597     524     402     393     268
                 209     129
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3050    3050 
   Size:       5902
 
EcoO109I rG'GnC_Cy

Cuts at:   2079    2079 
   Size:       5902
 
EcoRI G'AATT_C

Cuts at:   2400    3008    3211    3589    2400 
   Size:        608     203     378    4713

  Fragments arranged by size: 

                4713     608     378     203
 
EcoRV GAT'ATC

Cuts at:   2692    2692 
   Size:       5902
 
FspI TGC'GCA

Cuts at:   4408    4408 
   Size:       5902
 
HaeII r_GCGC'y

Cuts at:   1678    2090    5049    1678 
   Size:        412    2959    2531

  Fragments arranged by size: 

                2959    2531     412
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2844    2849    2876    2881    4543 
   Size:         32     135      32    1272       5      27       5    1662

Cuts at:   4543    4575    4617    4649    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1662    1272     135      42      32      32      32
                  32      27       5       5
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2353    2409    3037    3246    3492     669 
   Size:        988     281     415      56     628     209     246    3079

  Fragments arranged by size: 

                3079     988     628     415     281     246     209      56
            
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5902
 
HpaI GTT'AAC

Cuts at:   3492    3492 
   Size:       5902
 
KasI G'GCGC_C

Cuts at:   2086    2086 
   Size:       5902
 
KpnI G_GTAC'C

Cuts at:   3227    3227 
   Size:       5902
 
MfeI C'AATT_G

Cuts at:   3501    3501 
   Size:       5902
 
MluI A'CGCG_T

Cuts at:   5778    5778 
   Size:       5902
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2507    5016    5200    1305 
   Size:       1202    2509     184    2007

  Fragments arranged by size: 

                2509    2007    1202     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2906      10 
   Size:         54    1425     343    1074    3006

  Fragments arranged by size: 

                3006    1425    1074     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    3860    4219    4378     518 
   Size:        648    2694     359     159    2042

  Fragments arranged by size: 

                2694    2042     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2052    2058    2986    3318    3995    5143    5388    1606 
   Size:        446       6     928     332     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     928     677     446     332     245       6
            
 
NarI GG'CG_CC

Cuts at:   2087    2087 
   Size:       5902
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    4411

  Fragments arranged by size: 

                4411     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5902
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5902
 
NotI GC'GGCC_GC

Cuts at:   3349    3349 
   Size:       5902
 
NspI r_CATG'y

Cuts at:   1132    1229    2116    3141    3260    4805    1132 
   Size:         97     887    1025     119    1545    2229

  Fragments arranged by size: 

                2229    1545    1025     887     119      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5902
 
PciI A'CATG_T

Cuts at:   4801    4801 
   Size:       5902
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5902
 
PfoI T'CCnGG_A

Cuts at:   3156    3156 
   Size:       5902
 
PmeI GTTT'AAAC

Cuts at:   3206    3206 
   Size:       5902
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3971    4003    5484    5516    3971 
   Size:         32    1481      32    4357

  Fragments arranged by size: 

                4357    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2079    2079 
   Size:       5902
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5902
 
PsiI TTA'TAA

Cuts at:   3472    3472 
   Size:       5902
 
PstI C_TGCA'G

Cuts at:    830    2302    3254     830 
   Size:       1472     952    3478

  Fragments arranged by size: 

                3478    1472     952
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       5902
 
PvuII CAG'CTG

Cuts at:   2986    2986 
   Size:       5902
 
SalI G'TCGA_C

Cuts at:   1936    3244    1936 
   Size:       1308    4594
 
SanDI GG'GwC_CC

Cuts at:   2079    2079 
   Size:       5902
 
SbfI CC_TGCA'GG

Cuts at:   3254    3254 
   Size:       5902
 
ScaI AGT'ACT

Cuts at:   4150    5862    4150 
   Size:       1712    4190
 
SfcI C'TryA_G

Cuts at:    826    1045    2169    2199    2298    2800    3250    4385    5066 
   Size:        219    1124      30      99     502     450    1135     681

Cuts at:   5066    5257     826 
   Size:        191    1471

  Fragments arranged by size: 

                1471    1135    1124     681     502     450     219     191
                  99      30
 
SfoI GGC'GCC

Cuts at:   2088    2088 
   Size:       5902
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5902
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5902
 
SmaI CCC'GGG

Cuts at:   1872    3229    1872 
   Size:       1357    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    4006    4907    5169     820 
   Size:        197     926    2063     901     262    1553

  Fragments arranged by size: 

                2063    1553     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    3693     493 
   Size:       3200    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5902
 
SphI G_CATG'C

Cuts at:   1132    2116    3141    3260    1132 
   Size:        984    1025     119    3774

  Fragments arranged by size: 

                3774    1025     984     119
 
SspI AAT'ATT

Cuts at:      5      52    2707    2731    3826       5 
   Size:         47    2655      24    1095    2081

  Fragments arranged by size: 

                2655    2081    1095      47      24
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5902
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2317    3341     513 
   Size:        552     939     313    1024    3074

  Fragments arranged by size: 

                3074    1024     939     552     313
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2394    3929    3946    4099    4284    4729     325 
   Size:       1255     814    1535      17     153     185     445    1498

  Fragments arranged by size: 

                1535    1498    1255     814     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4148    5860      96 
   Size:        274      80      33      51    1443    2171    1712     138

  Fragments arranged by size: 

                2171    1712    1443     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2751    2783    5862    5894    2751 
   Size:         32    3079      32    2759

  Fragments arranged by size: 

                3079    2759      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5902
 
XbaI T'CTAG_A

Cuts at:   3238    3238 
   Size:       5902
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2372    2710    2372 
   Size:        338    5564
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5902
 
XmaI C'CCGG_G

Cuts at:   1870    3227    1870 
   Size:       1357    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4031    4031 
   Size:       5902
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3710     276 
   Size:         53      83     186     936    2176    2468

  Fragments arranged by size: 

                2468    2176     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbeI     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NarI     NcoI     NdeI     NheI     NotI     NspI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI     PstI     PvuI    PvuII     SalI    SanDI     SbfI     ScaI
     SfcI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI     FalI     FseI    FspAI     NaeI   NgoMIV     NruI
     NsiI     PacI     PmlI   PspOMI    PspXI     PsrI    RsrII     SacI
    SacII     SapI    SexAI     SfiI     SrfI     SwaI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com