################################## # NO CUT ENZYMES ( 99 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AviII BbrPI BbvCI BcgI BfrI BglII BsaBI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrDI BsrFI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HpaI KpnI KspI MamI MfeI MluI MroI MunI NaeI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 62 enzymes) # ################################### AatII 694 GACGTC GACGT^C AcsI 1079 AAATTC [GA]^AATT[CT] AflIII 308 ACGTGT A^C[GA][CT]GT Alw44I 965 GTGCAC G^TGCAC ApaI 137 GGGCCC GGGCC^C ApaLI 965 GTGCAC G^TGCAC ApoI 1079 AAATTC [GA]^AATT[CT] Asp700I 1404 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 694 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 1574 CCCGGG C^[CT]CG[GA]G AvrII 1419 CCTAGG C^CTAGG BalI 1619 TGGCCA TGG^CCA BamHI 196 GGATCC G^GATCC BbeI 1540 GGCGCC GGCGC^C BbsI 700 GTCTTC GAAGAC(2/6) BciVI 553 GGATAC GTATCC(6/5) BglI 1373 GCCACTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1419 CCTAGG C^CTAGG BlpI 1333 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1333 GCTGAGC GC^T[ACGT]AGC BpuAI 700 GTCTTC GAAGAC(2/6) BsaAI 307 TACGTG [CT]AC^GT[GA] BsaWI 1412 TCCGGT [AT]^CCGG[AT] BsmI 472 GAATGC GAATGC(1/-1) BsoBI 1574 CCCGGG C^[CT]CG[GA]G Bsp1407I 542 TGTACA T^GTACA BspHI 1188 TCATGA T^CATGA BspMI 908 ACCTGC ACCTGC(4/8) BsrBI 780 CCGCTC CCGCTC(-3/-3) BsrGI 542 TGTACA T^GTACA BssSI 64 CACGAG CACGAG(-5/-1) BstBI 608 TTCGAA TT^CGAA BstXI 1622 CCAACACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1333 GCTGAGC GC^T[ACGT]AGC DraIII 981 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 1619 TGGCCA [CT]^GGCC[GA] HgaI 59 GCGTC GACGC(5/10) KasI 1540 GGCGCC G^GCGCC MluNI 1619 TGGCCA TGG^CCA MscI 1619 TGGCCA TGG^CCA MseI 1490 TTAA T^TAA MslI 636 CATCCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 535 CAGCTG C[AC]G^C[GT]G NarI 1540 GGCGCC GG^CGCC NheI 1365 GCTAGC G^CTAGC NspI 244 GCATGT [GA]CATG^[CT] NspV 608 TTCGAA TT^CGAA PflFI 694 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1032 AGGACCC [GA]G^G[AT]CC[CT] PvuII 535 CAGCTG CAG^CTG RcaI 1188 TCATGA T^CATGA SfoI 1540 GGCGCC GGC^GCC SfuI 608 TTCGAA TT^CGAA SmaI 1574 CCCGGG CCC^GGG SspBI 542 TGTACA T^GTACA TfiI 1638 GATTC G^A[AT]TC Tru9I 1490 TTAA T^TAA Tsp45I 1612 GTCAC ^GT[GC]AC Tth111I 694 GACGTCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 1574 CCCGGG C^CCGGG XmaI 1574 CCCGGG C^CCGGG XmnI 1404 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 42 CGCG CG^CG 626 CGCG BanI 814 GGTGCC G^G[CT][GA]CC 1540 GGCGCC BclI 300 TGATCA T^GATCA 1218 TGATCA BcnI 1574 CCCGG CC^[GC]GG 1575 CCGGG BpmI 865 CTGGAG CTGGAG(16/14) 973 CTGGAG BseRI 595 CTCCTC GAGGAG(10/8) 1633 GAGGAG BsgI 621 CTGCAC GTGCAG(16/14) 1596 GTGCAG BsmAI 254 GAGAC GTCTC(1/5) 340 GTCTC BstUI 42 CGCG CG^CG 626 CGCG BstYI 196 GGATCC [GA]^GATC[CT] 1193 AGATCC FauI 1370 CCCGC CCCGC(4/6) 43 GCGGG FbaI 300 TGATCA T^GATCA 1218 TGATCA HaeII 1456 GGCGCT [GA]GCGC^[CT] 1540 GGCGCC HapII 1413 CCGG C^CGG 1575 CCGG HincII 525 GTCAAC GT[CT]^[GA]AC 1263 GTTGAC HindII 525 GTCAAC GT[CT]^[GA]AC 1263 GTTGAC HindIII 1087 AAGCTT A^AGCTT 1465 AAGCTT HpaII 1413 CCGG C^CGG 1575 CCGG MflI 196 GGATCC [GA]^GATC[CT] 1193 AGATCC MspI 1413 CCGG C^CGG 1575 CCGG MvnI 42 CGCG CG^CG 626 CGCG NciI 1574 CCCGG CC^[GC]GG 1575 CCGGG PstI 1383 CTGCAG CTGCA^G 1477 CTGCAG SacI 384 GAGCTC GAGCT^C 1272 GAGCTC SfaNI 1041 GATGC GCATC(5/9) 80 GCATC StuI 724 AGGCCT AGG^CCT 843 AGGCCT TaqI 13 TCGA T^CGA 609 TCGA TthHB8I 13 TCGA T^CGA 609 TCGA XhoII 196 GGATCC [GA]^GATC[CT] 1193 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaII BanII BbvI BfaI BmyI BsaHI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI Hin1I HinP1I HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI Tsp509I TspRI