(Circular) (Six-base) MAPSORT of: pFN21AE9547.seq Check: 9294 from: 1 to: 6364 pFN21AE9547 6364 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2703 4174 278 Size: 53 83 186 936 1167 1471 2468 Fragments arranged by size: 2468 1471 1167 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3115 4427 5811 6079 1632 Size: 1483 1312 1384 268 1917 Fragments arranged by size: 1917 1483 1384 1312 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6167 AflIII A'CryG_T Cuts at: 1203 2313 5263 6240 1203 Size: 1110 2950 977 1327 Fragments arranged by size: 2950 1327 1110 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6364 AlwNI CAG_nnn'CTG Cuts at: 2033 2412 3396 4107 5679 2033 Size: 379 984 711 1572 2718 Fragments arranged by size: 2718 1572 984 711 379 ApaI G_GGCC'C Cuts at: 2146 2146 Size: 6364 ApaLI G'TGCA_C Cuts at: 2970 4421 5577 2970 Size: 1451 1156 3757 Fragments arranged by size: 3757 1451 1156 ApoI r'AATT_y Cuts at: 1493 3084 3673 3899 4051 6160 1493 Size: 1591 589 226 152 2109 1697 Fragments arranged by size: 2109 1697 1591 589 226 152 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 3579 3689 1870 Size: 73 1636 110 4545 Fragments arranged by size: 4545 1636 110 73 AvrII C'CTAG_G Cuts at: 3424 3424 Size: 6364 BamHI G'GATC_C Cuts at: 2201 3694 4061 2201 Size: 1493 367 4504 Fragments arranged by size: 4504 1493 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2819 3545 3685 5140 618 Size: 325 217 627 1032 726 140 1455 1842 Fragments arranged by size: 1842 1455 1032 726 627 325 217 140 BanII G_rGCy'C Cuts at: 1391 2146 2236 2393 2877 3281 3683 1391 Size: 755 90 157 484 404 402 4072 Fragments arranged by size: 4072 755 484 404 402 157 90 BbeI G_GCGC'C Cuts at: 3549 3549 Size: 6364 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2698 928 Size: 971 799 4594 Fragments arranged by size: 4594 971 799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4555 4589 1903 Size: 34 2618 34 3678 Fragments arranged by size: 3678 2618 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2552 4256 5466 2552 Size: 1704 1210 3450 Fragments arranged by size: 3450 1704 1210 BclI T'GATC_A Cuts at: 1548 2305 3223 1548 Size: 757 918 4689 Fragments arranged by size: 4689 918 757 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3384 4975 136 Size: 107 122 71 631 605 1712 1591 1525 Fragments arranged by size: 1712 1591 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 3339 3781 3339 Size: 442 5922 Bme1580I G_kGCm'C Cuts at: 1790 2146 2974 4425 5581 1790 Size: 356 828 1451 1156 2573 Fragments arranged by size: 2573 1451 1156 828 356 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6364 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3364 4095 5053 Size: 416 53 83 399 24 1947 731 958 Cuts at: 5053 442 Size: 1753 Fragments arranged by size: 1947 1753 958 731 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3374 1056 Size: 2318 4046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2282 2282 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2891 2999 5024 1239 Size: 1652 108 2025 2579 Fragments arranged by size: 2579 2025 1652 108 Bpu10I CC'TnA_GC Cuts at: 2392 2421 2392 Size: 29 6335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2231 2252 3074 4489 5354 5652 2231 Size: 21 822 1415 865 298 2943 Fragments arranged by size: 2943 1415 865 822 298 21 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5027 882 Size: 617 3528 2219 Fragments arranged by size: 3528 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2314 4155 493 Size: 711 1110 1841 2702 Fragments arranged by size: 2702 1841 1110 711 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2064 2700 3546 4171 Size: 53 83 186 936 531 636 846 625 Cuts at: 4171 4553 5188 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 846 636 635 625 531 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3417 4797 5195 5469 5616 6027 3417 Size: 1380 398 274 147 411 3754 Fragments arranged by size: 3754 1380 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 1178 Size: 30 165 30 1302 30 4807 Fragments arranged by size: 4807 1302 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2591 3653 1186 Size: 1405 1062 3897 Fragments arranged by size: 3897 1405 1062 BseYI C'CCAG_C Cuts at: 2011 3541 3549 5567 6063 2011 Size: 1530 8 2018 496 2312 Fragments arranged by size: 2312 2018 1530 496 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2611 3622 1163 Size: 573 875 1011 3905 Fragments arranged by size: 3905 1011 875 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3814 4575 5603 1540 Size: 275 186 1813 761 1028 2301 Fragments arranged by size: 2301 1813 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 2393 2974 3281 4425 4510 5581 2135 Size: 258 581 307 1144 85 1071 2918 Fragments arranged by size: 2918 1144 1071 581 307 258 85 BsmI GAATG_Cn' Cuts at: 2483 3875 3968 2483 Size: 1392 93 4879 Fragments arranged by size: 4879 1392 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2146 2236 2393 2877 2974 3281 Size: 399 345 11 90 157 484 97 307 Cuts at: 3281 3683 4425 4510 5581 1391 Size: 402 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 402 399 345 307 157 97 90 85 11 BspEI T'CCGG_A Cuts at: 6027 6027 Size: 6364 BspHI T'CATG_A Cuts at: 3193 4253 5983 3193 Size: 1060 1730 3574 Fragments arranged by size: 3574 1730 1060 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2922 3719 844 Size: 2078 797 3489 Fragments arranged by size: 3489 2078 797 BsrBI CCG'CTC Cuts at: 1394 1823 2787 4251 1394 Size: 429 964 1464 3507 Fragments arranged by size: 3507 1464 964 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5008 5195 1700 Size: 110 3198 187 2869 Fragments arranged by size: 3198 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2547 96 Size: 2451 3913 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6364 BssSI C'ACGA_G Cuts at: 2069 4424 5436 2069 Size: 2355 1012 2997 Fragments arranged by size: 2997 2355 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 2614 5168 2614 Size: 2554 3810 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3634 2011 Size: 1623 4741 BstYI r'GATC_y Cuts at: 1972 2201 3198 3694 4061 4446 4463 5915 6001 Size: 229 997 496 367 385 17 1452 86 Cuts at: 6001 6013 6359 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 997 496 385 367 346 229 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2079 2451 2553 3621 3633 513 Size: 552 1014 372 102 1068 12 3244 Fragments arranged by size: 3244 1068 1014 552 372 102 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5625 Fragments arranged by size: 5625 683 56 BtsI GCAGTG_nn' Cuts at: 2410 2623 3610 3880 4674 4694 2410 Size: 213 987 270 794 20 4080 Fragments arranged by size: 4080 987 794 270 213 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6364 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3668 3668 Size: 6364 DraIII CAC_nnn'GTG Cuts at: 2991 2991 Size: 6364 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5371 809 Size: 507 4055 1802 Fragments arranged by size: 4055 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3624 3811 4700 Size: 54 1425 50 275 18 1794 187 889 Cuts at: 4700 6329 8 Size: 1629 43 Fragments arranged by size: 1794 1629 1425 889 275 187 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3811 1537 Size: 275 1999 4090 Fragments arranged by size: 4090 1999 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 2588 3209 3645 4294 1353 Size: 1026 209 621 436 649 3423 Fragments arranged by size: 3423 1026 649 621 436 209 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4951 5325 5471 387 Size: 1064 138 3362 374 146 1280 Fragments arranged by size: 3362 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2391 3279 2391 Size: 888 5476 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2891 2999 3115 4427 5024 5811 6079 Size: 393 1259 108 116 1312 597 787 268 Cuts at: 6079 1239 Size: 1524 Fragments arranged by size: 1524 1312 1259 787 597 393 268 116 108 EcoNI CCTnn'n_nnAGG Cuts at: 2037 2122 2210 2037 Size: 85 88 6191 Fragments arranged by size: 6191 88 85 EcoO109I rG'GnC_Cy Cuts at: 2030 2143 2154 2400 3038 2030 Size: 113 11 246 638 5356 Fragments arranged by size: 5356 638 246 113 11 EcoRI G'AATT_C Cuts at: 3673 4051 3673 Size: 378 5986 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 3465 3549 5511 1678 Size: 1787 84 1962 2531 Fragments arranged by size: 2531 1962 1787 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2095 2127 2290 2322 3384 Size: 32 135 32 523 32 163 32 1062 Cuts at: 3384 3416 5005 5037 5079 5111 1373 Size: 32 1589 32 42 32 2626 Fragments arranged by size: 2626 1589 1062 523 163 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2532 3270 3708 3954 669 Size: 988 281 594 738 438 246 3079 Fragments arranged by size: 3079 988 738 594 438 281 246 HindIII A'AGCT_T Cuts at: 748 3092 3470 748 Size: 2344 378 3642 Fragments arranged by size: 3642 2344 378 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 3545 3545 Size: 6364 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5478 5662 1305 Size: 4173 184 2007 Fragments arranged by size: 4173 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3626 10 Size: 54 1425 343 1794 2748 Fragments arranged by size: 2748 1794 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2645 4322 4681 4840 518 Size: 648 1479 1677 359 159 2042 Fragments arranged by size: 2042 1677 1479 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2542 3780 4457 5605 5850 1606 Size: 936 1238 677 1148 245 2120 Fragments arranged by size: 2120 1238 1148 936 677 245 NarI GG'CG_CC Cuts at: 3546 3546 Size: 6364 NcoI C'CATG_G Cuts at: 513 1065 2079 3621 3633 513 Size: 552 1014 1542 12 3244 Fragments arranged by size: 3244 1542 1014 552 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NheI G'CTAG_C Cuts at: 1052 3370 1052 Size: 2318 4046 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 2253 3722 5267 1132 Size: 97 1024 1469 1545 2229 Fragments arranged by size: 2229 1545 1469 1024 97 PasI CC'CwG_GG Cuts at: 1348 2097 2147 2955 1348 Size: 749 50 808 4757 Fragments arranged by size: 4757 808 749 50 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6364 PfoI T'CCnGG_A Cuts at: 2335 2335 Size: 6364 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3038 3038 Size: 6364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3141 3934 3141 Size: 793 5571 PspOMI G'GGCC_C Cuts at: 2142 2142 Size: 6364 PstI C_TGCA'G Cuts at: 830 3392 3486 3716 830 Size: 2562 94 230 3478 Fragments arranged by size: 3478 2562 230 94 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 PvuII CAG'CTG Cuts at: 2542 2542 Size: 6364 SacI G_AGCT'C Cuts at: 2393 3281 2393 Size: 888 5476 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2404 3388 3482 3712 4847 5528 5719 Size: 219 1359 984 94 230 1135 681 191 Cuts at: 5719 826 Size: 1471 Fragments arranged by size: 1471 1359 1135 984 681 230 219 191 94 SfoI GGC'GCC Cuts at: 3547 3547 Size: 6364 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3581 3691 1872 Size: 1709 110 4545 Fragments arranged by size: 4545 1709 110 SmlI C'TyrA_G Cuts at: 820 1017 1943 2210 2267 3089 4468 5369 5631 Size: 197 926 267 57 822 1379 901 262 Cuts at: 5631 820 Size: 1553 Fragments arranged by size: 1553 1379 926 901 822 267 262 197 57 SnaBI TAC'GTA Cuts at: 493 4155 493 Size: 3662 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 2731 2850 1517 Size: 1214 119 5031 Fragments arranged by size: 5031 1214 119 StyI C'CwwG_G Cuts at: 513 1065 2079 2372 3424 3621 3633 3803 513 Size: 552 1014 293 1052 197 12 170 3074 Fragments arranged by size: 3074 1052 1014 552 293 197 170 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2736 2807 4391 4408 4561 4746 5191 Size: 1255 1156 71 1584 17 153 185 445 Cuts at: 5191 325 Size: 1498 Fragments arranged by size: 1584 1498 1255 1156 445 185 153 71 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2238 2547 2686 Size: 274 80 33 51 1443 261 309 139 Cuts at: 2686 4610 6322 96 Size: 1924 1712 138 Fragments arranged by size: 1924 1712 1443 309 274 261 139 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2869 2901 6324 6356 2869 Size: 32 3423 32 2877 Fragments arranged by size: 3423 2877 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2702 1316 Size: 1386 4978 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3579 3689 1870 Size: 1709 110 4545 Fragments arranged by size: 4545 1709 110 XmnI GAAnn'nnTTC Cuts at: 3413 4493 3413 Size: 1080 5284 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2701 4172 276 Size: 53 83 186 936 1167 1471 2468 Fragments arranged by size: 2468 1471 1167 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI