(Linear) (Six-base) MAPSORT of: KIEE9547.seq Check: 2375 from: 1 to: 1659 KIEE9547.orf With 182 enzymes: SgfI * December 27, 2011 16:42 .. AatII G_ACGT'C Cuts at: 0 698 1659 Size: 698 961 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1110 1659 Size: 1110 549 AflIII A'CryG_T Cuts at: 0 308 1659 Size: 308 1351 AlwNI CAG_nnn'CTG Cuts at: 0 28 407 1391 1659 Size: 28 379 984 268 Fragments arranged by size: 984 379 268 28 ApaI G_GGCC'C Cuts at: 0 141 1659 Size: 141 1518 ApaLI G'TGCA_C Cuts at: 0 965 1659 Size: 965 694 ApoI r'AATT_y Cuts at: 0 1079 1659 Size: 1079 580 AvaI C'yCGr_G Cuts at: 0 1574 1659 Size: 1574 85 AvrII C'CTAG_G Cuts at: 0 1419 1659 Size: 1419 240 BamHI G'GATC_C Cuts at: 0 196 1659 Size: 196 1463 BanI G'GyrC_C Cuts at: 0 814 1540 1659 Size: 814 726 119 Fragments arranged by size: 814 726 119 BanII G_rGCy'C Cuts at: 0 141 231 388 872 1276 1659 Size: 141 90 157 484 404 383 Fragments arranged by size: 484 404 383 157 141 90 BbeI G_GCGC'C Cuts at: 0 1544 1659 Size: 1544 115 BbsI GAAGACnn'nnnn_ Cuts at: 0 693 1659 Size: 693 966 BciVI GTATCCnnnnn_n' Cuts at: 0 547 1659 Size: 547 1112 BclI T'GATC_A Cuts at: 0 300 1218 1659 Size: 300 918 441 Fragments arranged by size: 918 441 300 BglI GCCn_nnn'nGGC Cuts at: 0 1379 1659 Size: 1379 280 BlpI GC'TnA_GC Cuts at: 0 1334 1659 Size: 1334 325 Bme1580I G_kGCm'C Cuts at: 0 141 969 1659 Size: 141 828 690 Fragments arranged by size: 828 690 141 BmrI ACTGGGnnnn_n' Cuts at: 0 1359 1659 Size: 1359 300 BmtI G_CTAG'C Cuts at: 0 1369 1659 Size: 1369 290 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 277 1659 Size: 277 1382 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 886 994 1659 Size: 886 108 665 Fragments arranged by size: 886 665 108 Bpu10I CC'TnA_GC Cuts at: 0 387 416 1659 Size: 387 29 1243 Fragments arranged by size: 1243 387 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 226 247 1069 1659 Size: 226 21 822 590 Fragments arranged by size: 822 590 226 21 BsaAI yAC'GTr Cuts at: 0 309 1659 Size: 309 1350 BsaHI Gr'CG_yC Cuts at: 0 59 695 1541 1659 Size: 59 636 846 118 Fragments arranged by size: 846 636 118 59 BsaWI w'CCGG_w Cuts at: 0 1412 1659 Size: 1412 247 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 700 730 1659 Size: 700 30 929 Fragments arranged by size: 929 700 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 586 1648 1659 Size: 586 1062 11 Fragments arranged by size: 1062 586 11 BseYI C'CCAG_C Cuts at: 0 6 1536 1544 1659 Size: 6 1530 8 115 Fragments arranged by size: 1530 115 8 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 606 1617 1659 Size: 606 1011 42 Fragments arranged by size: 1011 606 42 BsiHKAI G_wGCw'C Cuts at: 0 130 388 969 1276 1659 Size: 130 258 581 307 383 Fragments arranged by size: 581 383 307 258 130 BsmI GAATG_Cn' Cuts at: 0 478 1659 Size: 478 1181 Bsp1286I G_dGCh'C Cuts at: 0 130 141 231 388 872 969 1276 1659 Size: 130 11 90 157 484 97 307 383 Fragments arranged by size: 484 383 307 157 130 97 90 11 BspHI T'CATG_A Cuts at: 0 1188 1659 Size: 1188 471 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 917 1659 Size: 917 742 BsrBI CCG'CTC Cuts at: 0 782 1659 Size: 782 877 BsrGI T'GTAC_A Cuts at: 0 542 1659 Size: 542 1117 BssSI C'ACGA_G Cuts at: 0 64 1659 Size: 64 1595 BstBI TT'CG_AA Cuts at: 0 609 1659 Size: 609 1050 BstXI CCAn_nnnn'nTGG Cuts at: 0 1629 1659 Size: 1629 30 BstYI r'GATC_y Cuts at: 0 196 1193 1659 Size: 196 997 466 Fragments arranged by size: 997 466 196 BtgI C'CryG_G Cuts at: 0 74 446 548 1616 1628 1659 Size: 74 372 102 1068 12 31 Fragments arranged by size: 1068 372 102 74 31 12 BtsI GCAGTG_nn' Cuts at: 0 405 618 1605 1659 Size: 405 213 987 54 Fragments arranged by size: 987 405 213 54 DraIII CAC_nnn'GTG Cuts at: 0 986 1659 Size: 986 673 EaeI y'GGCC_r Cuts at: 0 1619 1659 Size: 1619 40 EarI CTCTTCn'nnn_ Cuts at: 0 374 583 1204 1640 1659 Size: 374 209 621 436 19 Fragments arranged by size: 621 436 374 209 19 EcoICRI GAG'CTC Cuts at: 0 386 1274 1659 Size: 386 888 385 Fragments arranged by size: 888 386 385 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 886 994 1110 1659 Size: 886 108 116 549 Fragments arranged by size: 886 549 116 108 EcoNI CCTnn'n_nnAGG Cuts at: 0 32 117 205 1659 Size: 32 85 88 1454 Fragments arranged by size: 1454 88 85 32 EcoO109I rG'GnC_Cy Cuts at: 0 25 138 149 395 1033 1659 Size: 25 113 11 246 638 626 Fragments arranged by size: 638 626 246 113 25 11 HaeII r_GCGC'y Cuts at: 0 1460 1544 1659 Size: 1460 84 115 Fragments arranged by size: 1460 115 84 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 90 122 285 317 1379 1411 1659 Size: 90 32 163 32 1062 32 248 Fragments arranged by size: 1062 248 163 90 32 32 32 HincII GTy'rAC Cuts at: 0 527 1265 1659 Size: 527 738 394 Fragments arranged by size: 738 527 394 HindIII A'AGCT_T Cuts at: 0 1087 1465 1659 Size: 1087 378 194 Fragments arranged by size: 1087 378 194 KasI G'GCGC_C Cuts at: 0 1540 1659 Size: 1540 119 MscI TGG'CCA Cuts at: 0 1621 1659 Size: 1621 38 MslI CAynn'nnrTG Cuts at: 0 640 1659 Size: 640 1019 MspA1I CmG'CkG Cuts at: 0 537 1659 Size: 537 1122 NarI GG'CG_CC Cuts at: 0 1541 1659 Size: 1541 118 NcoI C'CATG_G Cuts at: 0 74 1616 1628 1659 Size: 74 1542 12 31 Fragments arranged by size: 1542 74 31 12 NheI G'CTAG_C Cuts at: 0 1365 1659 Size: 1365 294 NspI r_CATG'y Cuts at: 0 248 1659 Size: 248 1411 PasI CC'CwG_GG Cuts at: 0 92 142 950 1659 Size: 92 50 808 709 Fragments arranged by size: 808 709 92 50 PfoI T'CCnGG_A Cuts at: 0 330 1659 Size: 330 1329 PpuMI rG'GwC_Cy Cuts at: 0 1033 1659 Size: 1033 626 PsiI TTA'TAA Cuts at: 0 1136 1659 Size: 1136 523 PspOMI G'GGCC_C Cuts at: 0 137 1659 Size: 137 1522 PstI C_TGCA'G Cuts at: 0 1387 1481 1659 Size: 1387 94 178 Fragments arranged by size: 1387 178 94 PvuII CAG'CTG Cuts at: 0 537 1659 Size: 537 1122 SacI G_AGCT'C Cuts at: 0 388 1276 1659 Size: 388 888 383 Fragments arranged by size: 888 388 383 SfcI C'TryA_G Cuts at: 0 399 1383 1477 1659 Size: 399 984 94 182 Fragments arranged by size: 984 399 182 94 SfoI GGC'GCC Cuts at: 0 1542 1659 Size: 1542 117 SmaI CCC'GGG Cuts at: 0 1576 1659 Size: 1576 83 SmlI C'TyrA_G Cuts at: 0 205 262 1084 1659 Size: 205 57 822 575 Fragments arranged by size: 822 575 205 57 StuI AGG'CCT Cuts at: 0 726 845 1659 Size: 726 119 814 Fragments arranged by size: 814 726 119 StyI C'CwwG_G Cuts at: 0 74 367 1419 1616 1628 1659 Size: 74 293 1052 197 12 31 Fragments arranged by size: 1052 293 197 74 31 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 731 802 1659 Size: 731 71 857 Fragments arranged by size: 857 731 71 TatI w'GTAC_w Cuts at: 0 233 542 681 1659 Size: 233 309 139 978 Fragments arranged by size: 978 309 233 139 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 864 896 1659 Size: 864 32 763 Fragments arranged by size: 864 763 32 Tth111I GACn'n_nGTC Cuts at: 0 697 1659 Size: 697 962 XmaI C'CCGG_G Cuts at: 0 1574 1659 Size: 1574 85 XmnI GAAnn'nnTTC Cuts at: 0 1408 1659 Size: 1408 251 ZraI GAC'GTC Cuts at: 0 696 1659 Size: 696 963 Enzymes that do cut: AatII AcuI AflIII AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI BciVI BclI BglI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI BstBI BstXI BstYI BtgI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I NarI NcoI NheI NspI PasI PfoI PpuMI PsiI PspOMI PstI PvuII SacI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XmaI XmnI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI AscI AseI AsiSI BaeI BbvCI BcgI BfrBI BglII BmgBI BsaI BsaBI BsiEI BsiWI BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI MluI MmeI NaeI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PmlI PpiI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XcmI XhoI