################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI AocI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MaeII MamI MfeI MluI MluNI MroI MscI MseI MspA1I MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 523 GTCTAC GT^[AC][GT]AC AccII 72 CGCG CG^CG AcsI 580 GAATTC [GA]^AATT[CT] AcyI 32 GGCGCC G[GA]^CG[CT]C AfaI 489 GTAC GT^AC AlwNI 587 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 580 GAATTC [GA]^AATT[CT] BbeI 32 GGCGCC GGCGC^C BfaI 262 CTAG C^TAG BglI 589 GCCACTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 32 GGCGCC G[GA]^CG[CT]C BspMI 370 GCAGGT ACCTGC(4/8) BsrBI 67 CCGCTC CCGCTC(-3/-3) BstUI 72 CGCG CG^CG BstXI 630 CCAGGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 585 CTCAG C^T[ACGT]AG DsaI 119 CCGTGG C^C[GA][CT]GG EcoNI 624 CCTCTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 580 GAATTC G^AATTC FauI 66 CCCGC CCCGC(4/6) Hin1I 32 GGCGCC G[GA]^CG[CT]C KasI 32 GGCGCC G^GCGCC MaeI 262 CTAG C^TAG MaeIII 463 GTCAC ^GT[ACGT]AC MslI 502 CACTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 72 CGCG CG^CG NarI 32 GGCGCC GG^CGCC PflMI 343 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 731 GACTC GAGTC(4/5) RsaI 489 GTAC GT^AC SapI 311 GAAGAGC GCTCTTC(1/4) SfaNI 142 GCATC GCATC(5/9) SfoI 32 GGCGCC GGC^GCC SmaI 467 CCCGGG CCC^GGG SmlI 700 CTCAAG C^T[CT][GA]AG SpeI 261 ACTAGT A^CTAGT Tsp45I 463 GTCAC ^GT[GC]AC Tsp509I 581 AATT ^AATT Van91I 343 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 467 CCCGGG C^CCGGG XmaI 467 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### Alw44I 281 GTGCAC G^TGCAC 499 GTGCAC ApaI 610 GGGCCC GGGCC^C 692 GGGCCC ApaLI 281 GTGCAC G^TGCAC 499 GTGCAC AspHI 281 GTGCAC G[AT]GC[AT]^C 499 GTGCAC AvaI 467 CCCGGG C^[CT]CG[GA]G 607 CTCGGG BamHI 237 GGATCC G^GATCC 674 GGATCC BanI 32 GGCGCC G^G[CT][GA]CC 49 GGCACC BpmI 420 CTCCAG CTGGAG(16/14) 574 CTGGAG BseRI 172 GAGGAG GAGGAG(10/8) 187 GAGGAG BsiHKAI 281 GTGCAC G[AT]GC[AT]^C 499 GTGCAC BsmFI 382 GGGAC GGGAC(10/14) 64 GTCCC BsoBI 467 CCCGGG C^[CT]CG[GA]G 607 CTCGGG BstF5I 340 CATCC GGATG(2/0) 549 GGATG BstYI 237 GGATCC [GA]^GATC[CT] 674 GGATCC EarI 733 CTCTTC CTCTTC(1/4) 311 GAAGAG FokI 340 CATCC GGATG(9/13) 549 GGATG HaeII 32 GGCGCC [GA]GCGC^[CT] 90 GGCGCT HphI 464 TCACC GGTGA(8/7) 719 TCACC Ksp632I 733 CTCTTC CTCTTC(1/4) 311 GAAGAG MflI 237 GGATCC [GA]^GATC[CT] 674 GGATCC PpuMI 197 AGGACCC [GA]G^G[AT]CC[CT] 661 GGGTCCT TaqI 273 TCGA T^CGA 309 TCGA TfiI 213 GAATC G^A[AT]TC 410 GAATC TspRI 386 CAGTG CA[GC]TG[ACGT][ACGT]^ 591 CACTG TthHB8I 273 TCGA T^CGA 309 TCGA XhoII 237 GGATCC [GA]^GATC[CT] 674 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DpnII DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI