################################## # NO CUT ENZYMES ( 156 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MfeI MflI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Van91I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AlwI 519 GATCC GGATC(4/5) AocI 873 CCTGAGG CC^T[ACGT]AGG AspI 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 575 CTCGAG C^[CT]CG[GA]G BbvI 66 GCAGC GCAGC(8/12) BglI 427 GCCCTGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 328 CTGGAG CTGGAG(16/14) BsaBI 594 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsoBI 575 CTCGAG C^[CT]CG[GA]G Bsp1407I 830 TGTACA T^GTACA BspHI 404 TCATGA T^CATGA BsrGI 830 TGTACA T^GTACA BsrI 150 ACTGG ACTGG(1/-1) BstEII 840 GGTGACC G^GT[ACGT]ACC BstPI 840 GGTGACC G^GT[ACGT]ACC BstXI 110 CCACCCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 873 CCTGAGG CC^T[ACGT]AGG CfoI 436 GCGC GCG^C DpnII 519 GATC ^GATC DsaI 350 CCATGG C^C[GA][CT]GG EarI 219 CTCTTC CTCTTC(1/4) Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoO65I 840 GGTGACC G^GT[ACGT]ACC Fnu4HI 66 GCAGC GC^[ACGT]GC HaeII 435 GGCGCT [GA]GCGC^[CT] HhaI 436 GCGC GCG^C HinP1I 436 GCGC G^CGC ItaI 66 GCAGC GC^[ACGT]GC Ksp632I 219 CTCTTC CTCTTC(1/4) MamI 594 GATATTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 519 GATC ^GATC MseI 581 TTAA T^TAA MwoI 427 GCCCTGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 350 CCATGG C^CATGG NdeII 519 GATC ^GATC NspI 251 ACATGC [GA]CATG^[CT] PaeR7I 575 CTCGAG C^TCGAG PflFI 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC RcaI 404 TCATGA T^CATGA Sau3AI 519 GATC ^GATC SfaNI 23 GCATC GCATC(5/9) SfcI 867 CTACAG C^T[GA][CT]AG SspBI 830 TGTACA T^GTACA StuI 707 AGGCCT AGG^CCT Tru9I 581 TTAA T^TAA TseI 66 GCAGC G^C[AT]GC Tsp509I 660 AATT ^AATT Tth111I 811 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 575 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AfaI 303 GTAC GT^AC 831 GTAC Asp700I 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 924 GAAAACTTTC BsaI 323 GAGACC GGTCTC(1/5) 769 GGTCTC BseRI 127 CTCCTC GAGGAG(10/8) 666 CTCCTC BsgI 388 CTGCAC GTGCAG(16/14) 506 GTGCAG BsmFI 6 GGGAC GGGAC(10/14) 682 GTCCC Cac8I 47 GCCTGC GC[ACGT]^[ACGT]GC 558 GCTGGC EcoT14I 350 CCATGG C^C[AT][AT]GG 642 CCTTGG HinfI 154 GACTC G^A[ACGT]TC 908 GAGTC HphI 591 GGTGA GGTGA(8/7) 840 GGTGA PleI 154 GACTC GAGTC(4/5) 908 GAGTC RsaI 303 GTAC GT^AC 831 GTAC SmlI 575 CTCGAG C^T[CT][GA]AG 676 CTCAAG StyI 350 CCATGG C^C[AT][AT]GG 642 CCTTGG TaqI 147 TCGA T^CGA 576 TCGA TthHB8I 147 TCGA T^CGA 576 TCGA XmnI 855 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 924 GAAAACTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AvaII BalI BanII BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cfr13I DdeI DraII EaeI EcoO109I EcoRII FokI HaeIII MaeIII MboII MluNI MnlI MscI MslI MvaI NlaIII NlaIV PpuMI Sau96I ScrFI Tsp45I TspRI