################################## # NO CUT ENZYMES ( 137 enzymes) # ################################## AatII AccI AccII AccIII AclI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AvaII AviII AvrII BamHI BbsI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HincII HindII HpaI KspI MaeI MamI MfeI MflI MluI MroI MunI MvnI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### Acc65I 631 GGTACC G^GTACC AcyI 66 GGCGCC G[GA]^CG[CT]C AflIII 727 ACATGT A^C[GA][CT]GT AlwNI 8 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG Asp700I 714 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 631 GGTACC G^GTACC AspHI 94 GTGCTC G[AT]GC[AT]^C BanII 163 GAGCCC G[GA]GC[CT]^C BbeI 66 GGCGCC GGCGC^C BbrPI 667 CACGTG CAC^GTG BbvCI 135 CCTCAGC CCTCAGC(-5/-2) BclI 527 TGATCA T^GATCA BsaAI 667 CACGTG [CT]AC^GT[GA] BsaHI 66 GGCGCC G[GA]^CG[CT]C BsiHKAI 94 GTGCTC G[AT]GC[AT]^C BsmAI 260 GTCTC GTCTC(1/5) BsmBI 259 CGTCTC CGTCTC(1/5) BsmFI 267 GTCCC GGGAC(10/14) BspLU11I 727 ACATGT A^CATGT BspMI 628 GCAGGT ACCTGC(4/8) BsrDI 33 CATTGC GCAATG(2/0) BsrFI 389 GCCGGC [GA]^CCGG[CT] CfoI 67 GCGC GCG^C Cfr10I 389 GCCGGC [GA]^CCGG[CT] DpnII 528 GATC ^GATC DraII 292 GGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 484 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 292 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRI 689 GAATTC G^AATTC FbaI 527 TGATCA T^GATCA HaeII 66 GGCGCC [GA]GCGC^[CT] HapII 390 CCGG C^CGG HhaI 67 GCGC GCG^C Hin1I 66 GGCGCC G[GA]^CG[CT]C HinP1I 67 GCGC G^CGC HindIII 805 AAGCTT A^AGCTT HpaII 390 CCGG C^CGG KasI 66 GGCGCC G^GCGCC KpnI 631 GGTACC GGTAC^C MaeII 668 ACGT A^CGT MboI 528 GATC ^GATC MseI 779 TTAA T^TAA MspI 390 CCGG C^CGG NaeI 389 GCCGGC GCC^GGC NarI 66 GGCGCC GG^CGCC NdeII 528 GATC ^GATC NgoMIV 389 GCCGGC G^CCGGC NspI 727 ACATGT [GA]CATG^[CT] PmaCI 667 CACGTG CAC^GTG PmlI 667 CACGTG CAC^GTG Sau3AI 528 GATC ^GATC SexAI 113 ACCTGGT A^CC[AT]GGT SfoI 66 GGCGCC GGC^GCC Tru9I 779 TTAA T^TAA XcmI 321 CCATCTGTTCGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 714 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 689 GAATTC [GA]^AATT[CT] 833 GAATTT AfaI 186 GTAC GT^AC 632 GTAC ApoI 689 GAATTC [GA]^AATT[CT] 833 GAATTT BalI 380 TGGCCA TGG^CCA 491 TGGCCA BanI 66 GGCGCC G^G[CT][GA]CC 631 GGTACC BmyI 94 GTGCTC G[AGT]GC[ACT]^C 163 GAGCCC BsgI 278 GTGCAG GTGCAG(16/14) 898 GTGCAG Bsp1286I 94 GTGCTC G[AGT]GC[ACT]^C 163 GAGCCC DdeI 38 CTGAG C^T[ACGT]AG 136 CTCAG DsaI 359 CCATGG C^C[GA][CT]GG 638 CCATGG EaeI 380 TGGCCA [CT]^GGCC[GA] 491 TGGCCA EcoT14I 359 CCATGG C^C[AT][AT]GG 638 CCATGG FauI 269 CCCGC CCCGC(4/6) 410 CCCGC HgaI 233 GCGTC GACGC(5/10) 448 GCGTC HphI 398 TCACC GGTGA(8/7) 467 TCACC MluNI 380 TGGCCA TGG^CCA 491 TGGCCA MscI 380 TGGCCA TGG^CCA 491 TGGCCA MslI 177 CACCCCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 408 CACCCGCATG MspA1I 149 CAGCTG C[AC]G^C[GT]G 674 CAGCTG NcoI 359 CCATGG C^CATGG 638 CCATGG PflMI 7 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 483 CCACACTGTGG PleI 255 GAGTC GAGTC(4/5) 417 GAGTC PstI 76 CTGCAG CTGCA^G 677 CTGCAG PvuII 149 CAGCTG CAG^CTG 674 CAGCTG RsaI 186 GTAC GT^AC 632 GTAC StyI 359 CCATGG C^C[AT][AT]GG 638 CCATGG TfiI 10 GAATC G^A[AT]TC 374 GATTC Tsp509I 690 AATT ^AATT 834 AATT Van91I 7 CCAGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 483 CCACACTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI BbvI BpmI BsaJI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I Cfr13I EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeIII MboII MnlI MvaI MwoI NlaIII NlaIV SapI Sau96I ScrFI SfaNI SfcI TseI Tsp45I TspRI