(Linear) (Six-base) MAPSORT of: KIEE1149.seq Check: 7234 from: 1 to: 1347 KIEE1149.orf With 182 enzymes: SgfI * July 15, 2010 18:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 241 1347 Size: 241 1106 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 519 932 1037 1347 Size: 519 413 105 310 Fragments arranged by size: 519 413 310 105 AfeI AGC'GCT Cuts at: 0 940 1347 Size: 940 407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1223 1347 Size: 1223 124 ApaI G_GGCC'C Cuts at: 0 38 1347 Size: 38 1309 AvaI C'yCGr_G Cuts at: 0 164 370 1347 Size: 164 206 977 Fragments arranged by size: 977 206 164 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 428 461 1347 Size: 428 33 886 Fragments arranged by size: 886 428 33 BamHI G'GATC_C Cuts at: 0 57 1347 Size: 57 1290 BanI G'GyrC_C Cuts at: 0 83 201 294 422 657 923 1347 Size: 83 118 93 128 235 266 424 Fragments arranged by size: 424 266 235 128 118 93 83 BanII G_rGCy'C Cuts at: 0 38 641 1347 Size: 38 603 706 Fragments arranged by size: 706 603 38 BbsI GAAGACnn'nnnn_ Cuts at: 0 718 1347 Size: 718 629 BbvCI CC'TCA_GC Cuts at: 0 1061 1347 Size: 1061 286 BglI GCCn_nnn'nGGC Cuts at: 0 134 287 1347 Size: 134 153 1060 Fragments arranged by size: 1060 153 134 Bme1580I G_kGCm'C Cuts at: 0 38 139 439 1347 Size: 38 101 300 908 Fragments arranged by size: 908 300 101 38 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 660 1347 Size: 660 687 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 274 366 415 634 982 1293 1347 Size: 121 153 92 49 219 348 311 54 Fragments arranged by size: 348 311 219 153 121 92 54 49 Bpu10I CC'TnA_GC Cuts at: 0 76 1061 1347 Size: 76 985 286 Fragments arranged by size: 985 286 76 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 220 250 406 436 974 1004 1347 Size: 220 30 156 30 538 30 343 Fragments arranged by size: 538 343 220 156 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 424 427 1018 1036 1078 1347 Size: 424 3 591 18 42 269 Fragments arranged by size: 591 424 269 42 18 3 BseYI C'CCAG_C Cuts at: 0 290 375 941 1347 Size: 290 85 566 406 Fragments arranged by size: 566 406 290 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 196 1347 Size: 196 1151 BsiHKAI G_wGCw'C Cuts at: 0 392 1347 Size: 392 955 Bsp1286I G_dGCh'C Cuts at: 0 38 139 392 439 641 1347 Size: 38 101 253 47 202 706 Fragments arranged by size: 706 253 202 101 47 38 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 241 1347 Size: 241 1106 BsrFI r'CCGG_y Cuts at: 0 86 128 145 419 1347 Size: 86 42 17 274 928 Fragments arranged by size: 928 274 86 42 17 BssSI C'ACGA_G Cuts at: 0 319 1347 Size: 319 1028 BstYI r'GATC_y Cuts at: 0 57 1347 Size: 57 1290 Bsu36I CC'TnA_GG Cuts at: 0 449 541 1347 Size: 449 92 806 Fragments arranged by size: 806 449 92 BtgI C'CryG_G Cuts at: 0 22 38 205 455 1347 Size: 22 16 167 250 892 Fragments arranged by size: 892 250 167 22 16 BtsI GCAGTG_nn' Cuts at: 0 298 1347 Size: 298 1049 DraIII CAC_nnn'GTG Cuts at: 0 930 1347 Size: 930 417 EaeI y'GGCC_r Cuts at: 0 143 403 470 1347 Size: 143 260 67 877 Fragments arranged by size: 877 260 143 67 EarI CTCTTCn'nnn_ Cuts at: 0 538 617 971 1013 1109 1347 Size: 538 79 354 42 96 238 Fragments arranged by size: 538 354 238 96 79 42 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 274 366 415 519 634 932 982 Size: 121 153 92 49 104 115 298 50 Cuts at: 982 1037 1293 1347 Size: 55 256 54 Fragments arranged by size: 298 256 153 121 115 104 92 55 54 50 49 EcoNI CCTnn'n_nnAGG Cuts at: 0 430 1347 Size: 430 917 EcoO109I rG'GnC_Cy Cuts at: 0 34 330 350 1347 Size: 34 296 20 997 Fragments arranged by size: 997 296 34 20 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1335 1347 Size: 1335 12 HaeII r_GCGC'y Cuts at: 0 313 420 942 1347 Size: 313 107 522 405 Fragments arranged by size: 522 405 313 107 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 478 510 1308 1340 1347 Size: 478 32 798 32 7 Fragments arranged by size: 798 478 32 32 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1313 1347 Size: 1313 34 MscI TGG'CCA Cuts at: 0 405 472 1347 Size: 405 67 875 Fragments arranged by size: 875 405 67 MslI CAynn'nnrTG Cuts at: 0 868 1347 Size: 868 479 MspA1I CmG'CkG Cuts at: 0 207 573 859 1170 1271 1347 Size: 207 366 286 311 101 76 Fragments arranged by size: 366 311 286 207 101 76 NaeI GCC'GGC Cuts at: 0 88 130 147 1347 Size: 88 42 17 1200 Fragments arranged by size: 1200 88 42 17 NdeI CA'TA_TG Cuts at: 0 812 1347 Size: 812 535 NgoMIV G'CCGG_C Cuts at: 0 86 128 145 1347 Size: 86 42 17 1202 Fragments arranged by size: 1202 86 42 17 NspI r_CATG'y Cuts at: 0 1056 1347 Size: 1056 291 PflMI CCAn_nnn'nTGG Cuts at: 0 573 576 1347 Size: 573 3 771 Fragments arranged by size: 771 573 3 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 178 210 1347 Size: 178 32 1137 Fragments arranged by size: 1137 178 32 PspOMI G'GGCC_C Cuts at: 0 34 1347 Size: 34 1313 PstI C_TGCA'G Cuts at: 0 278 329 669 1347 Size: 278 51 340 678 Fragments arranged by size: 678 340 278 51 PvuII CAG'CTG Cuts at: 0 573 859 1170 1271 1347 Size: 573 286 311 101 76 Fragments arranged by size: 573 311 286 101 76 SacII CC_GC'GG Cuts at: 0 208 1347 Size: 208 1139 SfcI C'TryA_G Cuts at: 0 274 325 594 665 1347 Size: 274 51 269 71 682 Fragments arranged by size: 682 274 269 71 51 SgrAI Cr'CCGG_yG Cuts at: 0 419 1347 Size: 419 928 SmaI CCC'GGG Cuts at: 0 166 1347 Size: 166 1181 SphI G_CATG'C Cuts at: 0 1056 1347 Size: 1056 291 SspI AAT'ATT Cuts at: 0 1140 1347 Size: 1140 207 StyI C'CwwG_G Cuts at: 0 113 1230 1347 Size: 113 1117 117 Fragments arranged by size: 1117 117 113 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 282 1347 Size: 282 1065 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 220 252 406 438 974 1006 1125 1157 Size: 220 32 154 32 536 32 119 32 Cuts at: 1157 1347 Size: 190 Fragments arranged by size: 536 220 190 154 119 32 32 32 32 XbaI T'CTAG_A Cuts at: 0 705 1347 Size: 705 642 XmaI C'CCGG_G Cuts at: 0 164 1347 Size: 164 1183 Enzymes that do cut: AarI AcuI AfeI AlfI ApaI AvaI BaeI BamHI BanI BanII BbsI BbvCI BglI Bme1580I BplI BpmI Bpu10I BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI PflMI PpiI PspOMI PstI PvuII SacII SfcI SgrAI SmaI SphI SspI StyI TaqII TstI XbaI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AflIII AgeI AhdI AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BbeI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NarI NcoI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SmlI SnaBI SpeI SrfI StuI SwaI TatI Tth111I XcmI XhoI XmnI ZraI