(Linear) (Six-base) MAPSORT of: KIEE5249.seq Check: 4499 from: 1 to: 546 KIEE5249.orf With 182 enzymes: SgfI * January 29, 2014 11:52 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 546 Size: 462 84 AhdI GACnn_n'nnGTC Cuts at: 0 38 546 Size: 38 508 AvaI C'yCGr_G Cuts at: 0 109 546 Size: 109 437 BanI G'GyrC_C Cuts at: 0 52 397 546 Size: 52 345 149 Fragments arranged by size: 345 149 52 BbeI G_GCGC'C Cuts at: 0 56 546 Size: 56 490 BbsI GAAGACnn'nnnn_ Cuts at: 0 213 546 Size: 213 333 BciVI GTATCCnnnnn_n' Cuts at: 0 197 546 Size: 197 349 Bme1580I G_kGCm'C Cuts at: 0 400 546 Size: 400 146 BsaHI Gr'CG_yC Cuts at: 0 53 546 Size: 53 493 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 512 546 Size: 512 34 BseYI C'CCAG_C Cuts at: 0 138 200 546 Size: 138 62 346 Fragments arranged by size: 346 138 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 256 546 Size: 256 290 Bsp1286I G_dGCh'C Cuts at: 0 400 546 Size: 400 146 BsrDI GCAATG_nn' Cuts at: 0 516 546 Size: 516 30 BsrGI T'GTAC_A Cuts at: 0 284 546 Size: 284 262 BssHII G'CGCG_C Cuts at: 0 417 546 Size: 417 129 BstAPI GCAn_nnn'nTGC Cuts at: 0 135 546 Size: 135 411 BstXI CCAn_nnnn'nTGG Cuts at: 0 528 546 Size: 528 18 Bsu36I CC'TnA_GG Cuts at: 0 391 546 Size: 391 155 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 342 546 Size: 342 204 EaeI y'GGCC_r Cuts at: 0 2 298 515 546 Size: 2 296 217 31 Fragments arranged by size: 296 217 31 2 EarI CTCTTCn'nnn_ Cuts at: 0 253 546 Size: 253 293 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 462 546 Size: 462 84 FspI TGC'GCA Cuts at: 0 47 546 Size: 47 499 HaeII r_GCGC'y Cuts at: 0 56 76 118 160 332 546 Size: 56 20 42 42 172 214 Fragments arranged by size: 214 172 56 42 42 20 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 24 30 56 370 402 546 Size: 24 6 26 314 32 144 Fragments arranged by size: 314 144 32 26 24 6 HincII GTy'rAC Cuts at: 0 378 546 Size: 378 168 KasI G'GCGC_C Cuts at: 0 52 546 Size: 52 494 MscI TGG'CCA Cuts at: 0 300 546 Size: 300 246 MspA1I CmG'CkG Cuts at: 0 132 270 546 Size: 132 138 276 Fragments arranged by size: 276 138 132 NarI GG'CG_CC Cuts at: 0 53 546 Size: 53 493 PshAI GACnn'nnGTC Cuts at: 0 241 546 Size: 241 305 PsiI TTA'TAA Cuts at: 0 410 546 Size: 410 136 PstI C_TGCA'G Cuts at: 0 131 546 Size: 131 415 PvuII CAG'CTG Cuts at: 0 132 270 546 Size: 132 138 276 Fragments arranged by size: 276 138 132 SfcI C'TryA_G Cuts at: 0 127 546 Size: 127 419 SfoI GGC'GCC Cuts at: 0 54 546 Size: 54 492 StuI AGG'CCT Cuts at: 0 490 546 Size: 490 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 192 546 Size: 192 354 TatI w'GTAC_w Cuts at: 0 284 546 Size: 284 262 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 525 546 Size: 525 21 Enzymes that do cut: AcuI AhdI AvaI BanI BbeI BbsI BciVI Bme1580I BsaHI BseRI BseYI BsgI Bsp1286I BsrDI BsrGI BssHII BstAPI BstXI Bsu36I BtgZI EaeI EarI Eco57MI FspI HaeII Hin4I HincII KasI MscI MspA1I NarI PshAI PsiI PstI PvuII SfcI SfoI StuI TaqII TatI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssSI BstBI BstEII BstYI BstZ17I BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI