Restriction map for
pFN21AE3264
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# NO CUT ENZYMES ( 86 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbrPI BciVI BclI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiWI Bsp1407I BspEI BsrBI
BsrGI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CpoI
DraI DraIII DrdI Eco81I EcoNI
EcoO65I EcoRV EcoT22I FbaI FspI
HindIII HpaI KpnI MamI MfeI
MflI MluI MroI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SalI SbfI
ScaI SexAI SfuI SpeI Sse8387I
SspBI StuI SwaI XbaI XhoII
XmnI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AclI 1784 AACGTT AA^CGTT
AcyI 174 GGCGCC G[GA]^CG[CT]C
AhdI 1866 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 498 GGATC GGATC(4/5)
AlwNI 223 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 20 AGCGCT AGC^GCT
ApaI 206 GGGCCC GGGCC^C
AspEI 1866 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 793 GAGCTC G[AT]GC[AT]^C
AspI 781 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 866 TGGCCA TGG^CCA
BbeI 174 GGCGCC GGCGC^C
BbvCI 57 CCTCAGC CCTCAGC(-5/-2)
BcgI 1289 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 174 GGCGCC G[GA]^CG[CT]C
BsiHKAI 793 GAGCTC G[AT]GC[AT]^C
BsmBI 1639 GAGACG CGTCTC(1/5)
BsmI 635 GAATGC GAATGC(1/-1)
BspDI 500 ATCGAT AT^CGAT
BspHI 906 TCATGA T^CATGA
BspLU11I 833 ACATGT A^CATGT
BsrDI 1375 GCAATG GCAATG(2/0)
BssHII 101 GCGCGC G^CGCGC
BssSI 1697 CTCGTG CACGAG(-5/-1)
ClaI 500 ATCGAT AT^CGAT
DpnII 499 GATC ^GATC
Eam1105I 1866 GACCCTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 20 AGCGCT AGC^GCT
EcoRI 923 GAATTC G^AATTC
FseI 120 GGCCGGCC GGCCGG^CC
Hin1I 174 GGCGCC G[GA]^CG[CT]C
HincII 1006 GTCAAC GT[CT]^[GA]AC
HindII 1006 GTCAAC GT[CT]^[GA]AC
KasI 174 GGCGCC G^GCGCC
KspI 1895 CCGCGG CCGC^GG
MboI 499 GATC ^GATC
MluNI 866 TGGCCA TGG^CCA
MscI 866 TGGCCA TGG^CCA
NarI 174 GGCGCC GG^CGCC
NcoI 863 CCATGG C^CATGG
NdeII 499 GATC ^GATC
PaeR7I 16 CTCGAG C^TCGAG
PflFI 781 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 1784 AACGTT AA^CGTT
RcaI 906 TCATGA T^CATGA
SacI 793 GAGCTC GAGCT^C
SacII 1895 CCGCGG CCGC^GG
SapI 9 GCTCTTC GCTCTTC(1/4)
Sau3AI 499 GATC ^GATC
SfiI 55 GGCCTCAGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 174 GGCGCC GGC^GCC
SgrAI 170 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 203 CCCGGG CCC^GGG
SmlI 16 CTCGAG C^T[CT][GA]AG
SnaBI 709 TACGTA TAC^GTA
SphI 1739 GCATGC GCATG^C
Tth111I 781 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 863 CCATGGCCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 16 CTCGAG C^TCGAG
XmaCI 203 CCCGGG C^CCGGG
XmaI 203 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AflIII 833 ACATGT A^C[GA][CT]GT
948 ACGTGT
BbsI 1684 GAAGAC GAAGAC(2/6)
1091 GTCTTC
BfaI 259 CTAG C^TAG
1105 CTAG
BglI 56 GCCTCAGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
177 GCCCGCCCGGC
BlpI 105 GCTCAGC GC^T[ACGT]AGC
295 GCTAAGC
Bpu1102I 105 GCTCAGC GC^T[ACGT]AGC
295 GCTAAGC
BpuAI 1684 GAAGAC GAAGAC(2/6)
1091 GTCTTC
BsaAI 709 TACGTA [CT]AC^GT[GA]
947 TACGTG
BsaI 1233 GAGACC GGTCTC(1/5)
1277 GGTCTC
BsgI 472 CTGCAC GTGCAG(16/14)
1747 GTGCAG
BspMI 227 ACCTGC ACCTGC(4/8)
1533 ACCTGC
BstXI 1266 CCATTTCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1673 CCAGTGAGGTGG
CelII 105 GCTCAGC GC^T[ACGT]AGC
295 GCTAAGC
EagI 184 CGGCCG C^GGCCG
602 CGGCCG
EclXI 184 CGGCCG C^GGCCG
602 CGGCCG
Eco52I 184 CGGCCG C^GGCCG
602 CGGCCG
EcoT14I 644 CCTTGG C^C[AT][AT]GG
863 CCATGG
FauI 178 CCCGC CCCGC(4/6)
209 CCCGC
HphI 617 TCACC GGTGA(8/7)
717 TCACC
MaeI 259 CTAG C^TAG
1105 CTAG
MseI 1145 TTAA T^TAA
1243 TTAA
NaeI 121 GCCGGC GCC^GGC
171 GCCGGC
NgoMIV 121 GCCGGC G^CCGGC
171 GCCGGC
PflMI 417 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1025 CCATCTGTTGG
PshAI 762 GACGTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1618 GACCTCAGTC
PvuII 889 CAGCTG CAG^CTG
1441 CAGCTG
SspI 1147 AATATT AAT^ATT
1474 AATATT
StyI 644 CCTTGG C^C[AT][AT]GG
863 CCATGG
TfiI 1389 GATTC G^A[AT]TC
1491 GATTC
Tru9I 1145 TTAA T^TAA
1243 TTAA
Van91I 417 CCAGACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1025 CCATCTGTTGG
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# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DraII
DsaI EaeI EarI EcoO109I EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeII MaeIII MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
NspI PleI PpuMI PstI RsaI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I