(Circular) (Six-base) MAPSORT of: pFN21AE3264.seq Check: 4902 from: 1 to: 6634 pFN21AE3264 6634 bp With 182 enzymes: SgfI * March 28, 2012 13:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4444 278 Size: 53 83 186 936 2908 2468 Fragments arranged by size: 2908 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3977 1937 Size: 2040 4594 Acc65I G'GTAC_C Cuts at: 3955 3955 Size: 6634 AclI AA'CG_TT Cuts at: 1563 3790 4761 5134 1563 Size: 2227 971 373 3063 Fragments arranged by size: 3063 2227 971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3831 3899 4697 6081 6349 1632 Size: 2199 68 798 1384 268 1917 Fragments arranged by size: 2199 1917 1384 798 268 68 AfeI AGC'GCT Cuts at: 2027 2027 Size: 6634 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6437 AflIII A'CryG_T Cuts at: 1203 2838 2953 5533 6510 1203 Size: 1635 115 2580 977 1327 Fragments arranged by size: 2580 1635 1327 977 115 AgeI A'CCGG_T Cuts at: 5465 5465 Size: 6634 AhdI GACnn_n'nnGTC Cuts at: 3876 5363 3876 Size: 1487 5147 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6634 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3767 1184 Size: 2583 4051 AlwNI CAG_nnn'CTG Cuts at: 2233 4377 5949 2233 Size: 2144 1572 2918 Fragments arranged by size: 2918 2144 1572 ApaI G_GGCC'C Cuts at: 2215 2215 Size: 6634 ApaLI G'TGCA_C Cuts at: 4691 5847 4691 Size: 1156 5478 ApoI r'AATT_y Cuts at: 1493 2366 2374 2928 3208 3943 4169 4321 6430 Size: 873 8 554 280 735 226 152 2109 Cuts at: 6430 1493 Size: 1697 Fragments arranged by size: 2109 1697 873 735 554 280 226 152 8 AseI AT'TA_AT Cuts at: 160 1032 5188 160 Size: 872 4156 1606 Fragments arranged by size: 4156 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6634 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2120 2208 3959 1870 Size: 73 78 99 88 1751 4545 Fragments arranged by size: 4545 1751 99 88 78 73 BamHI G'GATC_C Cuts at: 3964 4331 3964 Size: 367 6267 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2179 2448 3955 5410 Size: 325 217 627 297 95 269 1507 1455 Cuts at: 5410 618 Size: 1842 Fragments arranged by size: 1842 1507 1455 627 325 297 269 217 95 BanII G_rGCy'C Cuts at: 1391 2215 2802 3678 3953 1391 Size: 824 587 876 275 4072 Fragments arranged by size: 4072 876 824 587 275 BbeI G_GCGC'C Cuts at: 2183 2183 Size: 6634 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3089 3696 928 Size: 971 1190 607 3866 Fragments arranged by size: 3866 1190 971 607 BbvCI CC'TCA_GC Cuts at: 2063 2063 Size: 6634 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3283 3317 4825 4859 1903 Size: 34 50 34 1262 34 1508 34 3678 Fragments arranged by size: 3678 1508 1262 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4526 5736 4526 Size: 1210 5424 BclI T'GATC_A Cuts at: 1548 1548 Size: 6634 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 2188 5245 Size: 107 122 71 631 605 395 121 3057 Cuts at: 5245 136 Size: 1525 Fragments arranged by size: 3057 1525 631 605 395 122 121 107 71 BglII A'GATC_T Cuts at: 6629 6629 Size: 6634 BlpI GC'TnA_GC Cuts at: 2111 2301 4051 2111 Size: 190 1750 4694 Fragments arranged by size: 4694 1750 190 Bme1580I G_kGCm'C Cuts at: 1790 2087 2215 4695 5851 1790 Size: 297 128 2480 1156 2573 Fragments arranged by size: 2573 2480 1156 297 128 BmgBI CAC'GTC Cuts at: 1318 2751 1318 Size: 1433 5201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2221 3672 4365 Size: 416 53 83 399 24 804 1451 693 Cuts at: 4365 5323 442 Size: 958 1753 Fragments arranged by size: 1753 1451 958 804 693 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6634 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2712 2712 Size: 6634 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2979 3428 3659 3758 5294 1239 Size: 1740 449 231 99 1536 2579 Fragments arranged by size: 2579 1740 1536 449 231 99 Bpu10I CC'TnA_GC Cuts at: 2063 2169 2063 Size: 106 6528 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4759 5624 5922 4759 Size: 865 298 5471 Fragments arranged by size: 5471 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3232 3288 5297 882 Size: 617 1733 56 2009 2219 Fragments arranged by size: 2219 2009 1733 617 56 BsaAI yAC'GTr Cuts at: 493 1204 2716 2954 4425 493 Size: 711 1512 238 1471 2702 Fragments arranged by size: 2702 1512 1471 711 238 BsaBI GATnn'nnATC Cuts at: 6628 6628 Size: 6634 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2180 4441 4823 5458 Size: 53 83 186 936 647 2261 382 635 Cuts at: 5458 275 Size: 1451 Fragments arranged by size: 2261 1451 936 647 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5067 5465 5739 5886 6297 5067 Size: 398 274 147 411 5404 Fragments arranged by size: 5404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2963 2993 1178 Size: 30 165 30 1560 30 4819 Fragments arranged by size: 4819 1560 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3014 3656 3761 1186 Size: 1828 642 105 4059 Fragments arranged by size: 4059 1828 642 105 BseYI C'CCAG_C Cuts at: 2039 2080 3369 5837 6333 2039 Size: 41 1289 2468 496 2340 Fragments arranged by size: 2468 2340 1289 496 41 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2462 3773 1163 Size: 573 726 1311 4024 Fragments arranged by size: 4024 1311 726 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2192 2557 2610 4084 4845 5873 Size: 275 186 191 365 53 1474 761 1028 Cuts at: 5873 1540 Size: 2301 Fragments arranged by size: 2301 1474 1028 761 365 275 191 186 53 BsiHKAI G_wGCw'C Cuts at: 2802 4695 4780 5851 2802 Size: 1893 85 1071 3585 Fragments arranged by size: 3585 1893 1071 85 BsmI GAATG_Cn' Cuts at: 2646 4145 4238 2646 Size: 1499 93 5042 Fragments arranged by size: 5042 1499 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3638 3638 Size: 6634 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2215 2802 3678 3953 4695 4780 Size: 399 297 128 587 876 275 742 85 Cuts at: 4780 5851 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 876 742 587 399 297 275 128 85 BspEI T'CCGG_A Cuts at: 6297 6297 Size: 6634 BspHI T'CATG_A Cuts at: 2911 4523 6253 2911 Size: 1612 1730 3292 Fragments arranged by size: 3292 1730 1612 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 3547 3989 844 Size: 1397 1306 442 3489 Fragments arranged by size: 3489 1397 1306 442 BsrBI CCG'CTC Cuts at: 1394 1823 4521 1394 Size: 429 2698 3507 Fragments arranged by size: 3507 2698 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3387 5129 5303 65 Size: 1183 2139 1742 174 1396 Fragments arranged by size: 2139 1742 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2126 2176 2624 5278 5465 1700 Size: 110 316 50 448 2654 187 2869 Fragments arranged by size: 2869 2654 448 316 187 110 50 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6634 BssHII G'CGCG_C Cuts at: 1925 2106 1925 Size: 181 6453 BssSI C'ACGA_G Cuts at: 3702 4694 5706 3702 Size: 992 1012 4630 Fragments arranged by size: 4630 1012 992 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6634 BstBI TT'CG_AA Cuts at: 5438 5438 Size: 6634 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6634 BstXI CCAn_nnnn'nTGG Cuts at: 3278 3685 3278 Size: 407 6227 BstYI r'GATC_y Cuts at: 1972 3964 4331 4716 4733 6185 6271 6283 6629 Size: 1992 367 385 17 1452 86 12 346 Cuts at: 6629 1972 Size: 1977 Fragments arranged by size: 1992 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2551 2635 2868 3900 513 Size: 552 1486 84 233 1032 3247 Fragments arranged by size: 3247 1486 1032 552 233 84 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2232 487 Size: 683 56 1006 4889 Fragments arranged by size: 4889 1006 683 56 BtsI GCAGTG_nn' Cuts at: 3577 4150 4944 4964 3577 Size: 573 794 20 5247 Fragments arranged by size: 5247 794 573 20 ClaI AT'CG_AT Cuts at: 1554 2506 1554 Size: 952 5682 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6599 DraI TTT'AAA Cuts at: 3938 3938 Size: 6634 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5641 809 Size: 507 4325 1802 Fragments arranged by size: 4325 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2189 2607 2871 Size: 54 1425 50 275 18 359 418 264 Cuts at: 2871 4081 4970 6599 8 Size: 1210 889 1629 43 Fragments arranged by size: 1629 1425 1210 889 418 359 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2189 2607 4081 1537 Size: 275 377 418 1474 4090 Fragments arranged by size: 4090 1474 418 377 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2021 2255 2337 2427 2679 3576 4564 1353 Size: 668 234 82 90 252 897 988 3423 Fragments arranged by size: 3423 988 897 668 252 234 90 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5221 5595 5741 387 Size: 1064 138 3632 374 146 1280 Fragments arranged by size: 3632 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2800 2800 Size: 6634 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2979 3428 3659 3758 3831 3899 4697 Size: 393 1347 449 231 99 73 68 798 Cuts at: 4697 5294 6081 6349 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1347 798 787 597 449 393 268 231 99 73 68 EcoO109I rG'GnC_Cy Cuts at: 2204 2731 3439 2204 Size: 527 708 5399 Fragments arranged by size: 5399 708 527 EcoRI G'AATT_C Cuts at: 2928 3943 4321 2928 Size: 1015 378 5241 Fragments arranged by size: 5241 1015 378 FseI GG_CCGG'CC Cuts at: 2130 2130 Size: 6634 FspI TGC'GCA Cuts at: 5140 5140 Size: 6634 HaeII r_GCGC'y Cuts at: 1678 2029 2100 2183 2199 5781 1678 Size: 351 71 83 16 3582 2531 Fragments arranged by size: 3582 2531 351 83 71 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2340 2370 2372 3809 Size: 32 135 32 766 2 30 2 1437 Cuts at: 3809 3841 3862 3894 5275 5307 5349 5381 1373 Size: 32 21 32 1381 32 42 32 2626 Fragments arranged by size: 2626 1437 1381 766 135 42 32 32 32 32 32 32 30 21 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3013 3978 4224 669 Size: 988 281 1075 965 246 3079 Fragments arranged by size: 3079 1075 988 965 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6634 HpaI GTT'AAC Cuts at: 4224 4224 Size: 6634 KasI G'GCGC_C Cuts at: 2179 2179 Size: 6634 KpnI G_GTAC'C Cuts at: 3959 3959 Size: 6634 MfeI C'AATT_G Cuts at: 4233 4233 Size: 6634 MluI A'CGCG_T Cuts at: 6510 6510 Size: 6634 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2941 3155 5748 5932 1305 Size: 1636 214 2593 184 2007 Fragments arranged by size: 2593 2007 1636 214 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2873 10 Size: 54 1425 343 1041 3771 Fragments arranged by size: 3771 1425 1041 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2888 3276 3749 4592 4951 5110 Size: 648 1374 348 388 473 843 359 159 Cuts at: 5110 518 Size: 2042 Fragments arranged by size: 2042 1374 843 648 473 388 359 348 159 MspA1I CmG'CkG Cuts at: 1606 2011 2067 2896 3448 3520 3902 4050 4727 Size: 405 56 829 552 72 382 148 677 Cuts at: 4727 5875 6120 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 552 405 382 245 148 72 56 NaeI GCC'GGC Cuts at: 2128 2178 2128 Size: 50 6584 NarI GG'CG_CC Cuts at: 2180 2180 Size: 6634 NcoI C'CATG_G Cuts at: 513 1065 2868 513 Size: 552 1803 4279 Fragments arranged by size: 4279 1803 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6634 NgoMIV G'CCGG_C Cuts at: 2126 2176 2126 Size: 50 6584 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6634 NotI GC'GGCC_GC Cuts at: 4081 4081 Size: 6634 NspI r_CATG'y Cuts at: 1132 1229 2842 3138 3748 3992 5537 1132 Size: 97 1613 296 610 244 1545 2229 Fragments arranged by size: 2229 1613 1545 610 296 244 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6634 PciI A'CATG_T Cuts at: 2838 5533 2838 Size: 2695 3939 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 3036 1108 Size: 1320 608 4706 Fragments arranged by size: 4706 1320 608 PmeI GTTT'AAAC Cuts at: 3938 3938 Size: 6634 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4703 4735 6216 6248 4703 Size: 32 1481 32 5089 Fragments arranged by size: 5089 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2204 2731 3439 2204 Size: 527 708 5399 Fragments arranged by size: 5399 708 527 PshAI GACnn'nnGTC Cuts at: 1613 2771 3627 1613 Size: 1158 856 4620 Fragments arranged by size: 4620 1158 856 PsiI TTA'TAA Cuts at: 4204 4204 Size: 6634 PspOMI G'GGCC_C Cuts at: 2211 2211 Size: 6634 PspXI vC'TCGA_Gb Cuts at: 2021 2021 Size: 6634 PstI C_TGCA'G Cuts at: 830 2166 2668 3438 3986 830 Size: 1336 502 770 548 3478 Fragments arranged by size: 3478 1336 770 548 502 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6634 PvuII CAG'CTG Cuts at: 2896 3448 2896 Size: 552 6082 SacI G_AGCT'C Cuts at: 2802 2802 Size: 6634 SacII CC_GC'GG Cuts at: 3903 3903 Size: 6634 SalI G'TCGA_C Cuts at: 1936 3976 1936 Size: 2040 4594 SanDI GG'GwC_CC Cuts at: 2204 2204 Size: 6634 SapI GCTCTTCn'nnn_ Cuts at: 2021 2021 Size: 6634 SbfI CC_TGCA'GG Cuts at: 3986 3986 Size: 6634 ScaI AGT'ACT Cuts at: 4882 6594 4882 Size: 1712 4922 SfcI C'TryA_G Cuts at: 826 1045 2162 2664 3219 3434 3982 5117 5798 Size: 219 1117 502 555 215 548 1135 681 Cuts at: 5798 5989 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1117 681 555 548 502 219 215 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2067 2067 Size: 6634 SfoI GGC'GCC Cuts at: 2181 2181 Size: 6634 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6634 SgrAI Cr'CCGG_yG Cuts at: 1700 2176 1700 Size: 476 6158 SmaI CCC'GGG Cuts at: 1872 2210 3961 1872 Size: 338 1751 4545 Fragments arranged by size: 4545 1751 338 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 4738 5639 5901 820 Size: 197 926 78 2717 901 262 1553 Fragments arranged by size: 2717 1553 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 2716 4425 493 Size: 2223 1709 2702 Fragments arranged by size: 2702 2223 1709 SpeI A'CTAG_T Cuts at: 152 152 Size: 6634 SphI G_CATG'C Cuts at: 1132 3748 3992 1132 Size: 2616 244 3774 Fragments arranged by size: 3774 2616 244 SspI AAT'ATT Cuts at: 5 52 3154 3481 4558 5 Size: 47 3102 327 1077 2081 Fragments arranged by size: 3102 2081 1077 327 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6634 StyI C'CwwG_G Cuts at: 513 1065 2649 2868 4073 513 Size: 552 1584 219 1205 3074 Fragments arranged by size: 3074 1584 1205 552 219 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2648 3620 4661 4678 4831 5016 5461 Size: 1255 1068 972 1041 17 153 185 445 Cuts at: 5461 325 Size: 1498 Fragments arranged by size: 1498 1255 1068 1041 972 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4880 6592 96 Size: 274 80 33 51 1443 2903 1712 138 Fragments arranged by size: 2903 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3112 3144 6594 6626 3112 Size: 32 3450 32 3120 Fragments arranged by size: 3450 3120 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2789 1316 Size: 1473 5161 XbaI T'CTAG_A Cuts at: 3970 3970 Size: 6634 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2875 2875 Size: 6634 XhoI C'TCGA_G Cuts at: 1943 2021 1943 Size: 78 6556 XmaI C'CCGG_G Cuts at: 1870 2208 3959 1870 Size: 338 1751 4545 Fragments arranged by size: 4545 1751 338 XmnI GAAnn'nnTTC Cuts at: 4763 4763 Size: 6634 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4442 276 Size: 53 83 186 936 2908 2468 Fragments arranged by size: 2908 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FspAI NruI NsiI PacI PfoI PmlI PsrI RsrII SexAI SrfI SwaI