################################## # NO CUT ENZYMES ( 113 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbeI BbsI BbvCI BclI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MluI MroI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 2134 GTCTAC GT^[AC][GT]AC AccII 243 CGCG CG^CG AhdI 1425 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2274 CCTGAGG CC^T[ACGT]AGG AspEI 1425 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 274 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 216 CACGTG CAC^GTG BcgI 812 GCAGCCGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 2332 GCCTCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 216 CACGTG [CT]AC^GT[GA] BsaBI 961 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 2469 GGTCTC GGTCTC(1/5) BsgI 1349 GTGCAG GTGCAG(16/14) Bsp1407I 1734 TGTACA T^GTACA BsrDI 2399 GCAATG GCAATG(2/0) BsrGI 1734 TGTACA T^GTACA BssSI 820 CTCGTG CACGAG(-5/-1) BstUI 243 CGCG CG^CG Bsu36I 2274 CCTGAGG CC^T[ACGT]AGG CfoI 242 GCGC GCG^C DraIII 654 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2447 CCGTGG C^C[GA][CT]GG Eam1105I 1425 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2274 CCTGAGG CC^T[ACGT]AGG EcoNI 48 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1358 CCAAGG C^C[AT][AT]GG HhaI 242 GCGC GCG^C HinP1I 242 GCGC G^CGC HincII 481 GTCAAC GT[CT]^[GA]AC HindII 481 GTCAAC GT[CT]^[GA]AC MamI 961 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 156 CAATTG C^AATTG MseI 773 TTAA T^TAA MunI 156 CAATTG C^AATTG MvnI 243 CGCG CG^CG NheI 1870 GCTAGC G^CTAGC PflFI 274 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 216 CACGTG CAC^GTG PmlI 216 CACGTG CAC^GTG PshAI 2205 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SspBI 1734 TGTACA T^GTACA StuI 1811 AGGCCT AGG^CCT StyI 1358 CCAAGG C^C[AT][AT]GG TaqI 686 TCGA T^CGA Tru9I 773 TTAA T^TAA Tth111I 274 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 686 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AcyI 706 GGCGTC G[GA]^CG[CT]C 1458 GACGCC AflIII 215 ACACGT A^C[GA][CT]GT 217 ACGTGT BciVI 221 GTATCC GTATCC(6/5) 438 GTATCC BfaI 538 CTAG C^TAG 1871 CTAG BsaHI 706 GGCGTC G[GA]^CG[CT]C 1458 GACGCC BstAPI 318 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 693 GCACCTCCTGC BstYI 966 AGATCC [GA]^GATC[CT] 1655 AGATCC EarI 1882 GAAGAG CTCTTC(1/4) 2361 GAAGAG FauI 809 CCCGC CCCGC(4/6) 244 GCGGG Hin1I 706 GGCGTC G[GA]^CG[CT]C 1458 GACGCC Ksp632I 1882 GAAGAG CTCTTC(1/4) 2361 GAAGAG MaeI 538 CTAG C^TAG 1871 CTAG MflI 966 AGATCC [GA]^GATC[CT] 1655 AGATCC PleI 1285 GAGTC GAGTC(4/5) 1431 GAGTC PstI 52 CTGCAG CTGCA^G 1691 CTGCAG SmlI 1818 CTCAAG C^T[CT][GA]AG 2412 CTCAAG TfiI 518 GAATC G^A[AT]TC 795 GATTC XhoII 966 AGATCC [GA]^GATC[CT] 1655 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 80 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DraII EaeI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HgaI HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PpuMI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI TseI Tsp45I Tsp509I TspRI XcmI