(Circular) (Six-base) MAPSORT of: pFN21AE1899.seq Check: 9332 from: 1 to: 7180 pFN21AE1899 7180 bp With 182 enzymes: SgfI * August 16, 2010 14:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2050 2111 2274 2050 Size: 61 163 6956 Fragments arranged by size: 6956 163 61 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4990 278 Size: 53 83 186 936 3454 2468 Fragments arranged by size: 3454 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4140 4523 1937 Size: 2203 383 4594 Fragments arranged by size: 4594 2203 383 Acc65I G'GTAC_C Cuts at: 4501 4501 Size: 7180 AclI AA'CG_TT Cuts at: 1563 5307 5680 1563 Size: 3744 373 3063 Fragments arranged by size: 3744 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2597 3487 5243 6627 6895 1632 Size: 965 890 1756 1384 268 1917 Fragments arranged by size: 1917 1756 1384 965 890 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6983 AflIII A'CryG_T Cuts at: 1203 2220 2222 6079 7056 1203 Size: 1017 2 3857 977 1327 Fragments arranged by size: 3857 1327 1017 977 2 AgeI A'CCGG_T Cuts at: 6011 6011 Size: 7180 AhdI GACnn_n'nnGTC Cuts at: 3435 5909 3435 Size: 2474 4706 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7180 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7180 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2538 2570 4433 4465 2538 Size: 32 1863 32 5253 Fragments arranged by size: 5253 1863 32 32 AlwNI CAG_nnn'CTG Cuts at: 2329 2332 3950 4923 6495 2329 Size: 3 1618 973 1572 3014 Fragments arranged by size: 3014 1618 1572 973 3 ApaI G_GGCC'C Cuts at: 2380 3363 4288 2380 Size: 983 925 5272 Fragments arranged by size: 5272 983 925 ApaLI G'TGCA_C Cuts at: 5237 6393 5237 Size: 1156 6024 ApoI r'AATT_y Cuts at: 1493 4489 4715 4867 6976 1493 Size: 2996 226 152 2109 1697 Fragments arranged by size: 2996 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5734 160 Size: 872 4702 1606 Fragments arranged by size: 4702 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7180 AvaI C'yCGr_G Cuts at: 1870 1943 2877 3587 4351 4505 1870 Size: 73 934 710 764 154 4545 Fragments arranged by size: 4545 934 764 710 154 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2209 2242 2209 Size: 33 7147 BamHI G'GATC_C Cuts at: 4510 4877 4510 Size: 367 6813 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3027 3370 3761 4345 4501 Size: 325 217 627 1240 343 391 584 156 Cuts at: 4501 5956 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1240 627 584 391 343 325 217 156 BanII G_rGCy'C Cuts at: 1391 2028 2100 2178 2345 2380 2620 2654 2688 Size: 637 72 78 167 35 240 34 34 Cuts at: 2688 3011 3363 4007 4288 4418 4499 1391 Size: 323 352 644 281 130 81 4072 Fragments arranged by size: 4072 644 637 352 323 281 240 167 130 81 78 72 35 34 34 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6209 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2806 2840 5371 5405 1903 Size: 34 869 34 2531 34 3678 Fragments arranged by size: 3678 2531 869 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2237 2454 5072 6282 2237 Size: 217 2618 1210 3135 Fragments arranged by size: 3135 2618 1210 217 BclI T'GATC_A Cuts at: 1548 1548 Size: 7180 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4343 5791 136 Size: 107 122 71 631 605 2671 1448 1525 Fragments arranged by size: 2671 1525 1448 631 605 122 107 71 BglII A'GATC_T Cuts at: 7175 7175 Size: 7180 BlpI GC'TnA_GC Cuts at: 4597 4597 Size: 7180 Bme1580I G_kGCm'C Cuts at: 1790 2380 2602 2903 3032 3363 3633 4288 5241 Size: 590 222 301 129 331 270 655 953 Cuts at: 5241 6397 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 953 655 590 331 301 270 222 129 BmgBI CAC'GTC Cuts at: 1318 2281 1318 Size: 963 6217 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3026 3938 4911 Size: 416 53 83 399 24 1609 912 973 Cuts at: 4911 5869 442 Size: 958 1753 Fragments arranged by size: 1753 1609 973 958 912 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3879 1056 Size: 2823 4357 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2452 2659 2966 3425 3593 3665 4064 4284 Size: 1213 207 307 459 168 72 399 220 Cuts at: 4284 4337 5840 1239 Size: 53 1503 2579 Fragments arranged by size: 2579 1503 1213 459 399 307 220 207 168 72 53 Bpu10I CC'TnA_GC Cuts at: 2850 3515 3639 2850 Size: 665 124 6391 Fragments arranged by size: 6391 665 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4402 5305 6170 6468 3808 Size: 594 903 865 298 4520 Fragments arranged by size: 4520 903 865 594 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4480 5843 882 Size: 617 2981 1363 2219 Fragments arranged by size: 2981 2219 1363 617 BsaAI yAC'GTr Cuts at: 493 1204 2223 4971 493 Size: 711 1019 2748 2702 Fragments arranged by size: 2748 2702 1019 711 BsaBI GATnn'nnATC Cuts at: 2970 7174 2970 Size: 4204 2976 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2712 3464 4987 5369 Size: 53 83 186 936 1179 752 1523 382 Cuts at: 5369 6004 275 Size: 635 1451 Fragments arranged by size: 1523 1451 1179 936 752 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5613 6011 6285 6432 6843 5613 Size: 398 274 147 411 5950 Fragments arranged by size: 5950 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2538 2568 2858 2888 3142 Size: 30 165 30 1135 30 290 30 254 Cuts at: 3142 3172 4433 4463 1178 Size: 30 1261 30 3895 Fragments arranged by size: 3895 1261 1135 290 254 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3134 3137 3140 3143 3269 1186 Size: 1948 3 3 3 126 5097 Fragments arranged by size: 5097 1948 126 3 3 3 BseYI C'CCAG_C Cuts at: 2318 2354 2654 2707 2809 3541 3606 6383 6879 Size: 36 300 53 102 732 65 2777 496 Cuts at: 6879 2318 Size: 2619 Fragments arranged by size: 2777 2619 732 496 300 102 65 53 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3375 1163 Size: 573 1639 4968 Fragments arranged by size: 4968 1639 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4630 5391 6419 1540 Size: 275 186 2629 761 1028 2301 Fragments arranged by size: 2629 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2178 2952 3328 4007 4035 4418 5241 5326 6397 Size: 774 376 679 28 383 823 85 1071 Cuts at: 6397 2178 Size: 2961 Fragments arranged by size: 2961 1071 823 774 679 383 376 85 28 BsmI GAATG_Cn' Cuts at: 4691 4784 4691 Size: 93 7087 BsmBI CGTCTCn'nnnn_ Cuts at: 2113 3455 3712 2113 Size: 1342 257 5581 Fragments arranged by size: 5581 1342 257 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2028 2100 2178 2345 2380 2602 2620 Size: 399 238 72 78 167 35 222 18 Cuts at: 2620 2654 2688 2903 2952 3011 3032 3328 3363 Size: 34 34 215 49 59 21 296 35 Cuts at: 3363 3633 4007 4035 4288 4418 4499 5241 5326 Size: 270 374 28 253 130 81 742 85 Cuts at: 5326 6397 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 374 296 270 253 238 222 215 167 130 85 81 78 72 59 49 35 35 34 34 28 21 18 BspEI T'CCGG_A Cuts at: 6843 6843 Size: 7180 BspHI T'CATG_A Cuts at: 5069 6799 5069 Size: 1730 5450 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 2111 2274 3252 4163 4535 844 Size: 1206 61 163 978 911 372 3489 Fragments arranged by size: 3489 1206 978 911 372 163 61 BsrBI CCG'CTC Cuts at: 1394 1823 5067 1394 Size: 429 3244 3507 Fragments arranged by size: 3507 3244 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4411 5675 5849 65 Size: 1183 3163 1264 174 1396 Fragments arranged by size: 3163 1396 1264 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5824 6011 1700 Size: 110 4014 187 2869 Fragments arranged by size: 4014 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3739 96 Size: 3643 3537 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7180 BssSI C'ACGA_G Cuts at: 2825 5240 6252 2825 Size: 2415 1012 3753 Fragments arranged by size: 3753 2415 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2329 2704 794 Size: 1535 375 5270 Fragments arranged by size: 5270 1535 375 BstBI TT'CG_AA Cuts at: 5984 5984 Size: 7180 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7180 BstXI CCAn_nnnn'nTGG Cuts at: 3011 3541 3571 3651 3778 4199 3011 Size: 530 30 80 127 421 5992 Fragments arranged by size: 5992 530 421 127 80 30 BstYI r'GATC_y Cuts at: 1972 2971 3660 4510 4877 5262 5279 6731 6817 Size: 999 689 850 367 385 17 1452 86 Cuts at: 6817 6829 7175 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 999 850 689 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4280 4280 Size: 7180 BtgI C'CryG_G Cuts at: 513 1065 2004 4452 513 Size: 552 939 2448 3241 Fragments arranged by size: 3241 2448 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2545 3097 3147 3460 487 Size: 683 56 1319 552 50 313 4207 Fragments arranged by size: 4207 1319 683 552 313 56 50 BtsI GCAGTG_nn' Cuts at: 3554 3847 3886 4135 4696 5490 5510 3554 Size: 293 39 249 561 794 20 5224 Fragments arranged by size: 5224 794 561 293 249 39 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7180 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7145 DraI TTT'AAA Cuts at: 4484 4484 Size: 7180 DraIII CAC_nnn'GTG Cuts at: 2664 2664 Size: 7180 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6187 809 Size: 507 4871 1802 Fragments arranged by size: 4871 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2739 3059 3561 Size: 54 1425 50 275 18 909 320 502 Cuts at: 3561 4168 4189 4204 4221 4627 5516 7145 8 Size: 607 21 15 17 406 889 1629 43 Fragments arranged by size: 1629 1425 909 889 607 502 406 320 275 54 50 43 21 18 17 15 EagI C'GGCC_G Cuts at: 1537 1812 4627 1537 Size: 275 2815 4090 Fragments arranged by size: 4090 2815 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3882 4361 5110 1353 Size: 2529 479 749 3423 Fragments arranged by size: 3423 2529 749 479 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2168 3656 5767 6141 6287 387 Size: 1064 138 579 1488 2111 374 146 1280 Fragments arranged by size: 2111 1488 1280 1064 579 374 146 138 EcoICRI GAG'CTC Cuts at: 2176 4005 4416 2176 Size: 1829 411 4940 Fragments arranged by size: 4940 1829 411 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2452 2597 2659 2966 3425 3487 3593 Size: 393 820 145 62 307 459 62 106 Cuts at: 3593 3665 4064 4284 4337 5243 5840 6627 6895 Size: 72 399 220 53 906 597 787 268 Cuts at: 6895 1239 Size: 1524 Fragments arranged by size: 1524 906 820 787 597 459 399 393 307 268 220 145 106 72 62 62 53 EcoNI CCTnn'n_nnAGG Cuts at: 2057 2057 Size: 7180 EcoO109I rG'GnC_Cy Cuts at: 2376 2377 2610 3103 3359 3791 3934 4257 4284 Size: 1 233 493 256 432 143 323 27 Cuts at: 4284 4285 4457 2376 Size: 1 172 5099 Fragments arranged by size: 5099 493 432 323 256 233 172 143 27 1 1 EcoRI G'AATT_C Cuts at: 4489 4867 4489 Size: 378 6802 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2362 2362 Size: 7180 FspI TGC'GCA Cuts at: 5686 5686 Size: 7180 HaeII r_GCGC'y Cuts at: 1678 6327 1678 Size: 4649 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2137 2169 2858 2890 3421 Size: 32 135 32 565 32 689 32 531 Cuts at: 3421 3453 3994 4026 4244 4276 5821 5853 5895 Size: 32 541 32 218 32 1545 32 42 Cuts at: 5895 5927 1373 Size: 32 2626 Fragments arranged by size: 2626 1545 689 565 541 531 218 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2488 4524 4770 669 Size: 988 281 550 2036 246 3079 Fragments arranged by size: 3079 2036 988 550 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7180 HpaI GTT'AAC Cuts at: 4770 4770 Size: 7180 KpnI G_GTAC'C Cuts at: 4505 4505 Size: 7180 MfeI C'AATT_G Cuts at: 2161 4779 2161 Size: 2618 4562 MluI A'CGCG_T Cuts at: 7056 7056 Size: 7180 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2136 2436 6294 6478 1305 Size: 831 300 3858 184 2007 Fragments arranged by size: 3858 2007 831 300 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3061 3563 4191 4206 4223 Size: 54 1425 343 1229 502 628 15 17 Cuts at: 4223 10 Size: 2967 Fragments arranged by size: 2967 1425 1229 628 502 343 54 17 15 MslI CAynn'nnrTG Cuts at: 518 1166 2403 3074 4466 5138 5497 5656 518 Size: 648 1237 671 1392 672 359 159 2042 Fragments arranged by size: 2042 1392 1237 672 671 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2194 2329 3695 4596 5273 6421 6666 1606 Size: 588 135 1366 901 677 1148 245 2120 Fragments arranged by size: 2120 1366 1148 901 677 588 245 135 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5689 Fragments arranged by size: 5689 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7180 NheI G'CTAG_C Cuts at: 1052 3875 1052 Size: 2823 4357 NotI GC'GGCC_GC Cuts at: 4627 4627 Size: 7180 NspI r_CATG'y Cuts at: 1132 1229 2366 3334 3746 4072 4538 6083 1132 Size: 97 1137 968 412 326 466 1545 2229 Fragments arranged by size: 2229 1545 1137 968 466 412 326 97 PasI CC'CwG_GG Cuts at: 1348 2373 3336 3378 3929 1348 Size: 1025 963 42 551 4599 Fragments arranged by size: 4599 1025 963 551 42 PciI A'CATG_T Cuts at: 6079 6079 Size: 7180 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 1108 Size: 902 6278 PfoI T'CCnGG_A Cuts at: 2841 3189 3237 2841 Size: 348 48 6784 Fragments arranged by size: 6784 348 48 PmeI GTTT'AAAC Cuts at: 4484 4484 Size: 7180 PmlI CAC'GTG Cuts at: 2223 2223 Size: 7180 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2538 2570 3218 3250 4433 4465 5249 5281 6762 Size: 32 648 32 1183 32 784 32 1481 Cuts at: 6762 6794 2538 Size: 32 2924 Fragments arranged by size: 2924 1481 1183 784 648 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3791 3934 4257 4457 3791 Size: 143 323 200 6514 Fragments arranged by size: 6514 323 200 143 PshAI GACnn'nnGTC Cuts at: 1613 4214 1613 Size: 2601 4579 PsiI TTA'TAA Cuts at: 4750 4750 Size: 7180 PspOMI G'GGCC_C Cuts at: 2376 3359 4284 2376 Size: 983 925 5272 Fragments arranged by size: 5272 983 925 PstI C_TGCA'G Cuts at: 830 2061 3700 4532 830 Size: 1231 1639 832 3478 Fragments arranged by size: 3478 1639 1231 832 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7180 PvuII CAG'CTG Cuts at: 2194 2329 3695 2194 Size: 135 1366 5679 Fragments arranged by size: 5679 1366 135 SacI G_AGCT'C Cuts at: 2178 4007 4418 2178 Size: 1829 411 4940 Fragments arranged by size: 4940 1829 411 SalI G'TCGA_C Cuts at: 1936 4522 1936 Size: 2586 4594 SanDI GG'GwC_CC Cuts at: 3791 3934 4457 3791 Size: 143 523 6514 Fragments arranged by size: 6514 523 143 SbfI CC_TGCA'GG Cuts at: 4532 4532 Size: 7180 ScaI AGT'ACT Cuts at: 5428 7140 5428 Size: 1712 5468 SfcI C'TryA_G Cuts at: 826 1045 2057 2593 2677 3696 4528 5663 6344 Size: 219 1012 536 84 1019 832 1135 681 Cuts at: 6344 6535 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1019 1012 832 681 536 219 191 84 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7180 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7180 SmaI CCC'GGG Cuts at: 1872 4507 1872 Size: 2635 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3823 4417 5284 6185 6447 820 Size: 197 926 1880 594 867 901 262 1553 Fragments arranged by size: 1880 1553 926 901 867 594 262 197 SnaBI TAC'GTA Cuts at: 493 4971 493 Size: 4478 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7180 SphI G_CATG'C Cuts at: 1132 4538 1132 Size: 3406 3774 SspI AAT'ATT Cuts at: 5 52 5104 5 Size: 47 5052 2081 Fragments arranged by size: 5052 2081 47 StuI AGG'CCT Cuts at: 1517 3818 1517 Size: 2301 4879 StyI C'CwwG_G Cuts at: 513 1065 2004 3363 4619 513 Size: 552 939 1359 1256 3074 Fragments arranged by size: 3074 1359 1256 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3779 5207 5224 5377 5562 6007 325 Size: 1255 2199 1428 17 153 185 445 1498 Fragments arranged by size: 2199 1498 1428 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3739 5426 7138 Size: 274 80 33 51 1443 1762 1687 1712 Cuts at: 7138 96 Size: 138 Fragments arranged by size: 1762 1712 1687 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3142 3174 7140 7172 3142 Size: 32 3966 32 3150 Fragments arranged by size: 3966 3150 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2282 1316 Size: 966 6214 XbaI T'CTAG_A Cuts at: 4516 4516 Size: 7180 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3778 4199 4397 3778 Size: 421 198 6561 Fragments arranged by size: 6561 421 198 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7180 XmaI C'CCGG_G Cuts at: 1870 4505 1870 Size: 2635 4545 XmnI GAAnn'nnTTC Cuts at: 5309 5309 Size: 7180 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4988 276 Size: 53 83 186 936 3454 2468 Fragments arranged by size: 3454 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BbvCI BfrBI BplI BsiWI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI