(Linear) (Six-base) MAPSORT of: KIEE1899.seq Check: 923 from: 1 to: 2475 KIEE1899.orf With 182 enzymes: SgfI * July 15, 2010 18:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 45 106 269 2475 Size: 45 61 163 2206 Fragments arranged by size: 2206 163 61 45 AccI GT'mk_AC Cuts at: 0 2135 2475 Size: 2135 340 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 592 1482 2475 Size: 592 890 993 Fragments arranged by size: 993 890 592 AflIII A'CryG_T Cuts at: 0 215 217 2475 Size: 215 2 2258 Fragments arranged by size: 2258 215 2 AhdI GACnn_n'nnGTC Cuts at: 0 1430 2475 Size: 1430 1045 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 533 565 2428 2460 2475 Size: 533 32 1863 32 15 Fragments arranged by size: 1863 533 32 32 15 AlwNI CAG_nnn'CTG Cuts at: 0 324 327 1945 2475 Size: 324 3 1618 530 Fragments arranged by size: 1618 530 324 3 ApaI G_GGCC'C Cuts at: 0 375 1358 2283 2475 Size: 375 983 925 192 Fragments arranged by size: 983 925 375 192 AvaI C'yCGr_G Cuts at: 0 872 1582 2346 2475 Size: 872 710 764 129 Fragments arranged by size: 872 764 710 129 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 204 237 2475 Size: 204 33 2238 Fragments arranged by size: 2238 204 33 BanI G'GyrC_C Cuts at: 0 1022 1365 1756 2340 2475 Size: 1022 343 391 584 135 Fragments arranged by size: 1022 584 391 343 135 BanII G_rGCy'C Cuts at: 0 23 95 173 340 375 615 649 683 Size: 23 72 78 167 35 240 34 34 Cuts at: 683 1006 1358 2002 2283 2413 2475 Size: 323 352 644 281 130 62 Fragments arranged by size: 644 352 323 281 240 167 130 78 72 62 35 34 34 23 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 801 835 2475 Size: 801 34 1640 Fragments arranged by size: 1640 801 34 BciVI GTATCCnnnnn_n' Cuts at: 0 232 449 2475 Size: 232 217 2026 Fragments arranged by size: 2026 232 217 BglI GCCn_nnn'nGGC Cuts at: 0 2338 2475 Size: 2338 137 Bme1580I G_kGCm'C Cuts at: 0 375 597 898 1027 1358 1628 2283 2475 Size: 375 222 301 129 331 270 655 192 Fragments arranged by size: 655 375 331 301 270 222 192 129 BmgBI CAC'GTC Cuts at: 0 276 2475 Size: 276 2199 BmrI ACTGGGnnnn_n' Cuts at: 0 1021 1933 2475 Size: 1021 912 542 Fragments arranged by size: 1021 912 542 BmtI G_CTAG'C Cuts at: 0 1874 2475 Size: 1874 601 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 447 654 961 1420 1588 1660 2059 2279 Size: 447 207 307 459 168 72 399 220 Cuts at: 2279 2332 2475 Size: 53 143 Fragments arranged by size: 459 447 399 307 220 207 168 143 72 53 Bpu10I CC'TnA_GC Cuts at: 0 845 1510 1634 2475 Size: 845 665 124 841 Fragments arranged by size: 845 841 665 124 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1803 2397 2475 Size: 1803 594 78 Fragments arranged by size: 1803 594 78 BsaI GGTCTCn'nnnn_ Cuts at: 0 2475 Size: 2475 BsaAI yAC'GTr Cuts at: 0 218 2475 Size: 218 2257 BsaBI GATnn'nnATC Cuts at: 0 965 2475 Size: 965 1510 BsaHI Gr'CG_yC Cuts at: 0 707 1459 2475 Size: 707 752 1016 Fragments arranged by size: 1016 752 707 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 533 563 853 883 1137 1167 2428 2458 Size: 533 30 290 30 254 30 1261 30 Cuts at: 2458 2475 Size: 17 Fragments arranged by size: 1261 533 290 254 30 30 30 30 17 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1129 1132 1135 1138 1264 2475 Size: 1129 3 3 3 126 1211 Fragments arranged by size: 1211 1129 126 3 3 3 BseYI C'CCAG_C Cuts at: 0 313 349 649 702 804 1536 1601 2475 Size: 313 36 300 53 102 732 65 874 Fragments arranged by size: 874 732 313 300 102 65 53 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1370 2475 Size: 1370 1105 BsiHKAI G_wGCw'C Cuts at: 0 173 947 1323 2002 2030 2413 2475 Size: 173 774 376 679 28 383 62 Fragments arranged by size: 774 679 383 376 173 62 28 BsmBI CGTCTCn'nnnn_ Cuts at: 0 108 1450 1707 2475 Size: 108 1342 257 768 Fragments arranged by size: 1342 768 257 108 Bsp1286I G_dGCh'C Cuts at: 0 23 95 173 340 375 597 615 649 Size: 23 72 78 167 35 222 18 34 Cuts at: 649 683 898 947 1006 1027 1323 1358 1628 Size: 34 215 49 59 21 296 35 270 Cuts at: 1628 2002 2030 2283 2413 2475 Size: 374 28 253 130 62 Fragments arranged by size: 374 296 270 253 222 215 167 130 78 72 62 59 49 35 35 34 34 28 23 21 18 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 45 106 269 1247 2158 2475 Size: 45 61 163 978 911 317 Fragments arranged by size: 978 911 317 163 61 45 BsrDI GCAATG_nn' Cuts at: 0 2406 2475 Size: 2406 69 BsrGI T'GTAC_A Cuts at: 0 1734 2475 Size: 1734 741 BssSI C'ACGA_G Cuts at: 0 820 2475 Size: 820 1655 BstAPI GCAn_nnn'nTGC Cuts at: 0 324 699 2475 Size: 324 375 1776 Fragments arranged by size: 1776 375 324 BstXI CCAn_nnnn'nTGG Cuts at: 0 1006 1536 1566 1646 1773 2194 2475 Size: 1006 530 30 80 127 421 281 Fragments arranged by size: 1006 530 421 281 127 80 30 BstYI r'GATC_y Cuts at: 0 966 1655 2475 Size: 966 689 820 Fragments arranged by size: 966 820 689 Bsu36I CC'TnA_GG Cuts at: 0 2275 2475 Size: 2275 200 BtgI C'CryG_G Cuts at: 0 2447 2475 Size: 2447 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 540 1092 1142 1455 2475 Size: 540 552 50 313 1020 Fragments arranged by size: 1020 552 540 313 50 BtsI GCAGTG_nn' Cuts at: 0 1549 1842 1881 2130 2475 Size: 1549 293 39 249 345 Fragments arranged by size: 1549 345 293 249 39 DraIII CAC_nnn'GTG Cuts at: 0 659 2475 Size: 659 1816 EaeI y'GGCC_r Cuts at: 0 734 1054 1556 2163 2184 2199 2216 2475 Size: 734 320 502 607 21 15 17 259 Fragments arranged by size: 734 607 502 320 259 21 17 15 EarI CTCTTCn'nnn_ Cuts at: 0 1877 2356 2475 Size: 1877 479 119 Fragments arranged by size: 1877 479 119 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 163 1651 2475 Size: 163 1488 824 Fragments arranged by size: 1488 824 163 EcoICRI GAG'CTC Cuts at: 0 171 2000 2411 2475 Size: 171 1829 411 64 Fragments arranged by size: 1829 411 171 64 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 447 592 654 961 1420 1482 1588 1660 Size: 447 145 62 307 459 62 106 72 Cuts at: 1660 2059 2279 2332 2475 Size: 399 220 53 143 Fragments arranged by size: 459 447 399 307 220 145 143 106 72 62 62 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 52 2475 Size: 52 2423 EcoO109I rG'GnC_Cy Cuts at: 0 371 372 605 1098 1354 1786 1929 2252 Size: 371 1 233 493 256 432 143 323 Cuts at: 2252 2279 2280 2452 2475 Size: 27 1 172 23 Fragments arranged by size: 493 432 371 323 256 233 172 143 27 23 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 357 2475 Size: 357 2118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 132 164 853 885 1416 1448 1989 2021 Size: 132 32 689 32 531 32 541 32 Cuts at: 2021 2239 2271 2475 Size: 218 32 204 Fragments arranged by size: 689 541 531 218 204 132 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 483 2475 Size: 483 1992 MfeI C'AATT_G Cuts at: 0 156 2475 Size: 156 2319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 131 431 2475 Size: 131 300 2044 Fragments arranged by size: 2044 300 131 MscI TGG'CCA Cuts at: 0 1056 1558 2186 2201 2218 2475 Size: 1056 502 628 15 17 257 Fragments arranged by size: 1056 628 502 257 17 15 MslI CAynn'nnrTG Cuts at: 0 398 1069 2461 2475 Size: 398 671 1392 14 Fragments arranged by size: 1392 671 398 14 MspA1I CmG'CkG Cuts at: 0 189 324 1690 2475 Size: 189 135 1366 785 Fragments arranged by size: 1366 785 189 135 NheI G'CTAG_C Cuts at: 0 1870 2475 Size: 1870 605 NspI r_CATG'y Cuts at: 0 361 1329 1741 2067 2475 Size: 361 968 412 326 408 Fragments arranged by size: 968 412 408 361 326 PasI CC'CwG_GG Cuts at: 0 368 1331 1373 1924 2475 Size: 368 963 42 551 551 Fragments arranged by size: 963 551 551 368 42 PfoI T'CCnGG_A Cuts at: 0 836 1184 1232 2475 Size: 836 348 48 1243 Fragments arranged by size: 1243 836 348 48 PmlI CAC'GTG Cuts at: 0 218 2475 Size: 218 2257 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 533 565 1213 1245 2428 2460 2475 Size: 533 32 648 32 1183 32 15 Fragments arranged by size: 1183 648 533 32 32 32 15 PpuMI rG'GwC_Cy Cuts at: 0 1786 1929 2252 2452 2475 Size: 1786 143 323 200 23 Fragments arranged by size: 1786 323 200 143 23 PshAI GACnn'nnGTC Cuts at: 0 2209 2475 Size: 2209 266 PspOMI G'GGCC_C Cuts at: 0 371 1354 2279 2475 Size: 371 983 925 196 Fragments arranged by size: 983 925 371 196 PstI C_TGCA'G Cuts at: 0 56 1695 2475 Size: 56 1639 780 Fragments arranged by size: 1639 780 56 PvuII CAG'CTG Cuts at: 0 189 324 1690 2475 Size: 189 135 1366 785 Fragments arranged by size: 1366 785 189 135 SacI G_AGCT'C Cuts at: 0 173 2002 2413 2475 Size: 173 1829 411 62 Fragments arranged by size: 1829 411 173 62 SanDI GG'GwC_CC Cuts at: 0 1786 1929 2452 2475 Size: 1786 143 523 23 Fragments arranged by size: 1786 523 143 23 SfcI C'TryA_G Cuts at: 0 52 588 672 1691 2475 Size: 52 536 84 1019 784 Fragments arranged by size: 1019 784 536 84 52 SmlI C'TyrA_G Cuts at: 0 1818 2412 2475 Size: 1818 594 63 Fragments arranged by size: 1818 594 63 StuI AGG'CCT Cuts at: 0 1813 2475 Size: 1813 662 StyI C'CwwG_G Cuts at: 0 1358 2475 Size: 1358 1117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1774 2475 Size: 1774 701 TatI w'GTAC_w Cuts at: 0 1734 2475 Size: 1734 741 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1137 1169 2475 Size: 1137 32 1306 Fragments arranged by size: 1306 1137 32 Tth111I GACn'n_nGTC Cuts at: 0 277 2475 Size: 277 2198 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1773 2194 2392 2475 Size: 1773 421 198 83 Fragments arranged by size: 1773 421 198 83 Enzymes that do cut: AarI AccI AcuI AflIII AhdI AloI AlwNI ApaI AvaI BaeI BanI BanII BcgI BciVI BglI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I HincII MfeI MmeI MscI MslI MspA1I NheI NspI PasI PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII Acc65I AclI AfeI AflII AgeI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbsI BbvCI BclI BfrBI BglII BlpI BplI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrFI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MluI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PciI PflMI PmeI PsiI PspXI PsrI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI