(Circular) (Six-base) MAPSORT of: pFN21AE5420.seq Check: 5571 from: 1 to: 5650 pFN21AE5420 5650 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3460 278 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2993 1937 Size: 1056 4594 Acc65I G'GTAC_C Cuts at: 2971 2971 Size: 5650 AclI AA'CG_TT Cuts at: 1563 3777 4150 1563 Size: 2214 373 3063 Fragments arranged by size: 3063 2214 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2277 2672 3713 5097 5365 1632 Size: 645 395 1041 1384 268 1917 Fragments arranged by size: 1917 1384 1041 645 395 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5453 AflIII A'CryG_T Cuts at: 1203 4549 5526 1203 Size: 3346 977 1327 Fragments arranged by size: 3346 1327 977 AgeI A'CCGG_T Cuts at: 4481 4481 Size: 5650 AhdI GACnn_n'nnGTC Cuts at: 2402 4379 2402 Size: 1977 3673 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5650 AlwNI CAG_nnn'CTG Cuts at: 3393 4965 3393 Size: 1572 4078 ApaLI G'TGCA_C Cuts at: 2594 3707 4863 2594 Size: 1113 1156 3381 Fragments arranged by size: 3381 1156 1113 ApoI r'AATT_y Cuts at: 1493 2959 3185 3337 5446 1493 Size: 1466 226 152 2109 1697 Fragments arranged by size: 2109 1697 1466 226 152 AseI AT'TA_AT Cuts at: 160 1032 4204 160 Size: 872 3172 1606 Fragments arranged by size: 3172 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 AvaI C'yCGr_G Cuts at: 1870 1943 2495 2736 2808 2975 1870 Size: 73 552 241 72 167 4545 Fragments arranged by size: 4545 552 241 167 73 72 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2008 2041 2008 Size: 33 5617 BamHI G'GATC_C Cuts at: 2980 3347 2980 Size: 367 5283 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2971 4426 618 Size: 325 217 627 1184 1455 1842 Fragments arranged by size: 1842 1455 1184 627 325 217 BanII G_rGCy'C Cuts at: 1391 2364 2730 2969 1391 Size: 973 366 239 4072 Fragments arranged by size: 4072 973 366 239 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2314 928 Size: 971 415 4264 Fragments arranged by size: 4264 971 415 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2214 2224 2248 2258 3841 3875 1903 Size: 34 277 10 24 10 1583 34 3678 Fragments arranged by size: 3678 1583 277 34 34 24 10 10 BciVI GTATCCnnnnn_n' Cuts at: 2036 3542 4752 2036 Size: 1506 1210 2934 Fragments arranged by size: 2934 1506 1210 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 5161 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4261 136 Size: 107 122 71 631 605 2589 1525 Fragments arranged by size: 2589 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5645 5645 Size: 5650 BlpI GC'TnA_GC Cuts at: 3067 3067 Size: 5650 Bme1580I G_kGCm'C Cuts at: 1790 2093 2598 3711 4867 1790 Size: 303 505 1113 1156 2573 Fragments arranged by size: 2573 1156 1113 505 303 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5650 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3381 4339 442 Size: 416 53 83 399 24 1964 958 1753 Fragments arranged by size: 1964 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5650 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2299 4310 1239 Size: 1060 2011 2579 Fragments arranged by size: 2579 2011 1060 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3775 4640 4938 3775 Size: 865 298 4487 Fragments arranged by size: 4487 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2484 2733 4313 882 Size: 617 985 249 1580 2219 Fragments arranged by size: 2219 1580 985 617 249 BsaAI yAC'GTr Cuts at: 493 1204 3441 493 Size: 711 2237 2702 Fragments arranged by size: 2702 2237 711 BsaBI GATnn'nnATC Cuts at: 2036 5644 2036 Size: 3608 2042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3457 3839 4474 275 Size: 53 83 186 936 1924 382 635 1451 Fragments arranged by size: 1924 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2697 4083 4481 4755 4902 5313 2697 Size: 1386 398 274 147 411 3034 Fragments arranged by size: 3034 1386 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5425 Fragments arranged by size: 5425 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2831 1186 Size: 1645 4005 BseYI C'CCAG_C Cuts at: 4853 5349 4853 Size: 496 5154 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2143 1163 Size: 573 407 4670 Fragments arranged by size: 4670 573 407 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2854 3100 3861 4889 1540 Size: 275 186 853 246 761 1028 2301 Fragments arranged by size: 2301 1028 853 761 275 246 186 BsiHKAI G_wGCw'C Cuts at: 2598 3711 3796 4867 2598 Size: 1113 85 1071 3381 Fragments arranged by size: 3381 1113 1071 85 BsmI GAATG_Cn' Cuts at: 3161 3254 3161 Size: 93 5557 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2364 2598 2730 2969 3711 3796 Size: 399 303 271 234 132 239 742 85 Cuts at: 3796 4867 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 303 271 239 234 132 85 BspEI T'CCGG_A Cuts at: 2697 5313 2697 Size: 2616 3034 BspHI T'CATG_A Cuts at: 3539 5269 3539 Size: 1730 3920 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2210 3005 844 Size: 1366 795 3489 Fragments arranged by size: 3489 1366 795 BsrBI CCG'CTC Cuts at: 1394 1823 3537 1394 Size: 429 1714 3507 Fragments arranged by size: 3507 1714 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2079 4145 4319 65 Size: 1183 831 2066 174 1396 Fragments arranged by size: 2066 1396 1183 831 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4294 4481 1700 Size: 110 2484 187 2869 Fragments arranged by size: 2869 2484 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5650 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5650 BssSI C'ACGA_G Cuts at: 3710 4722 3710 Size: 1012 4638 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5650 BstBI TT'CG_AA Cuts at: 4454 4454 Size: 5650 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5650 BstXI CCAn_nnnn'nTGG Cuts at: 2086 2086 Size: 5650 BstYI r'GATC_y Cuts at: 1972 2732 2980 3347 3732 3749 5201 5287 5299 Size: 760 248 367 385 17 1452 86 12 Cuts at: 5299 5645 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 760 385 367 346 248 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2829 513 Size: 552 1764 3334 Fragments arranged by size: 3334 1764 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2551 487 Size: 683 56 1325 3586 Fragments arranged by size: 3586 1325 683 56 BtsI GCAGTG_nn' Cuts at: 2091 2909 3166 3960 3980 2091 Size: 818 257 794 20 3761 Fragments arranged by size: 3761 818 794 257 20 ClaI AT'CG_AT Cuts at: 1554 2244 2692 1554 Size: 690 448 4512 Fragments arranged by size: 4512 690 448 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5615 DraI TTT'AAA Cuts at: 2954 2954 Size: 5650 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4657 809 Size: 507 3341 1802 Fragments arranged by size: 3341 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2925 3097 3986 Size: 54 1425 50 275 18 1095 172 889 Cuts at: 3986 5615 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1095 889 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3097 1537 Size: 275 1285 4090 Fragments arranged by size: 4090 1285 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3580 1353 Size: 2227 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4237 4611 4757 387 Size: 1064 138 2648 374 146 1280 Fragments arranged by size: 2648 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 2299 2672 3713 4310 5097 5365 Size: 393 645 22 373 1041 597 787 268 Cuts at: 5365 1239 Size: 1524 Fragments arranged by size: 1524 1041 787 645 597 393 373 268 22 EcoO109I rG'GnC_Cy Cuts at: 2199 2310 2812 2835 2199 Size: 111 502 23 5014 Fragments arranged by size: 5014 502 111 23 EcoRI G'AATT_C Cuts at: 2959 3337 2959 Size: 378 5272 FspI TGC'GCA Cuts at: 4156 4156 Size: 5650 HaeII r_GCGC'y Cuts at: 1678 4797 1678 Size: 3119 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 2717 2749 4291 Size: 32 135 32 816 32 297 32 1542 Cuts at: 4291 4323 4365 4397 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1542 816 297 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2994 3240 669 Size: 988 281 1056 246 3079 Fragments arranged by size: 3079 1056 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5650 HpaI GTT'AAC Cuts at: 3240 3240 Size: 5650 KpnI G_GTAC'C Cuts at: 2975 2975 Size: 5650 MfeI C'AATT_G Cuts at: 3249 3249 Size: 5650 MluI A'CGCG_T Cuts at: 5526 5526 Size: 5650 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2874 4764 4948 1305 Size: 1569 1890 184 2007 Fragments arranged by size: 2007 1890 1569 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3828 Fragments arranged by size: 3828 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2084 2792 3608 3967 4126 518 Size: 648 843 75 708 816 359 159 2042 Fragments arranged by size: 2042 843 816 708 648 359 159 75 MspA1I CmG'CkG Cuts at: 1606 3066 3743 4891 5136 1606 Size: 1460 677 1148 245 2120 Fragments arranged by size: 2120 1460 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2829 513 Size: 552 1764 3334 Fragments arranged by size: 3334 1764 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5650 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5650 NotI GC'GGCC_GC Cuts at: 3097 3097 Size: 5650 NspI r_CATG'y Cuts at: 1132 1229 3008 4553 1132 Size: 97 1779 1545 2229 Fragments arranged by size: 2229 1779 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5650 PciI A'CATG_T Cuts at: 4549 4549 Size: 5650 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5650 PmeI GTTT'AAAC Cuts at: 2954 2954 Size: 5650 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3719 3751 5232 5264 3719 Size: 32 1481 32 4105 Fragments arranged by size: 4105 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2199 2812 2835 2199 Size: 613 23 5014 Fragments arranged by size: 5014 613 23 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5650 PsiI TTA'TAA Cuts at: 3220 3220 Size: 5650 PstI C_TGCA'G Cuts at: 830 2440 3002 830 Size: 1610 562 3478 Fragments arranged by size: 3478 1610 562 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5650 SalI G'TCGA_C Cuts at: 1936 2992 1936 Size: 1056 4594 SanDI GG'GwC_CC Cuts at: 2812 2835 2812 Size: 23 5627 SbfI CC_TGCA'GG Cuts at: 3002 3002 Size: 5650 ScaI AGT'ACT Cuts at: 3898 5610 3898 Size: 1712 3938 SfcI C'TryA_G Cuts at: 826 1045 2046 2436 2683 2870 2998 4133 4814 Size: 219 1001 390 247 187 128 1135 681 Cuts at: 4814 5005 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1001 681 390 247 219 191 187 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5650 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5650 SmaI CCC'GGG Cuts at: 1872 2497 2810 2977 1872 Size: 625 313 167 4545 Fragments arranged by size: 4545 625 313 167 SmlI C'TyrA_G Cuts at: 820 1017 1943 3754 4655 4917 820 Size: 197 926 1811 901 262 1553 Fragments arranged by size: 1811 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3441 493 Size: 2948 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5650 SphI G_CATG'C Cuts at: 1132 3008 1132 Size: 1876 3774 SspI AAT'ATT Cuts at: 5 52 3574 5 Size: 47 3522 2081 Fragments arranged by size: 3522 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5650 StyI C'CwwG_G Cuts at: 513 1065 2829 3089 513 Size: 552 1764 260 3074 Fragments arranged by size: 3074 1764 552 260 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2905 3677 3694 3847 4032 4477 325 Size: 1255 1325 772 17 153 185 445 1498 Fragments arranged by size: 1498 1325 1255 772 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3896 5608 96 Size: 274 80 33 51 1443 1919 1712 138 Fragments arranged by size: 1919 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5610 5642 5610 Size: 32 5618 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5650 XbaI T'CTAG_A Cuts at: 2986 2986 Size: 5650 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5650 XmaI C'CCGG_G Cuts at: 1870 2495 2808 2975 1870 Size: 625 313 167 4545 Fragments arranged by size: 4545 625 313 167 XmnI GAAnn'nnTTC Cuts at: 3779 3779 Size: 5650 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3458 276 Size: 53 83 186 936 1924 2468 Fragments arranged by size: 2468 1924 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI