(Circular) (Six-base) MAPSORT of: pFN21AE5632.seq Check: 4596 from: 1 to: 6175 pFN21AE5632 6175 bp With 182 enzymes: SgfI * December 8, 2014 13:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3470 3518 1937 Size: 1533 48 4594 Fragments arranged by size: 4594 1533 48 Acc65I G'GTAC_C Cuts at: 3109 3496 3109 Size: 387 5788 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4238 5622 5890 1632 Size: 2606 1384 268 1917 Fragments arranged by size: 2606 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5978 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 4904 4904 Size: 6175 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AlwNI CAG_nnn'CTG Cuts at: 2549 3242 3266 3918 5490 2549 Size: 693 24 652 1572 3234 Fragments arranged by size: 3234 1572 693 652 24 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 2220 2569 2887 3484 3710 3862 5971 1493 Size: 727 349 318 597 226 152 2109 1697 Fragments arranged by size: 2109 1697 727 597 349 318 226 152 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 3500 1870 Size: 73 1557 4545 Fragments arranged by size: 4545 1557 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2976 3009 2976 Size: 33 6142 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2968 3109 3496 4951 618 Size: 325 217 627 1181 141 387 1455 1842 Fragments arranged by size: 1842 1455 1181 627 387 325 217 141 BanII G_rGCy'C Cuts at: 1391 3494 1391 Size: 2103 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2025 2942 3096 3131 928 Size: 971 126 917 154 35 3972 Fragments arranged by size: 3972 971 917 154 126 35 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4067 5277 4067 Size: 1210 4965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2834 6170 2834 Size: 3336 2839 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 4236 5392 1790 Size: 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3460 3906 4864 Size: 416 53 83 399 24 2043 446 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 2043 1753 958 446 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3381 3381 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2052 3114 3165 4835 1239 Size: 813 1062 51 1670 2579 Fragments arranged by size: 2579 1670 1062 813 51 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3239 3263 4838 882 Size: 617 1740 24 1575 2219 Fragments arranged by size: 2219 1740 1575 617 24 BsaAI yAC'GTr Cuts at: 493 1204 2648 3966 493 Size: 711 1444 1318 2702 Fragments arranged by size: 2702 1444 1318 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2050 2080 1178 Size: 30 165 30 647 30 5273 Fragments arranged by size: 5273 647 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6175 BseYI C'CCAG_C Cuts at: 3200 3340 5378 5874 3200 Size: 140 2038 496 3501 Fragments arranged by size: 3501 2038 496 140 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2766 1163 Size: 573 1030 4572 Fragments arranged by size: 4572 1030 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3474 3625 4386 5414 1540 Size: 275 186 1473 151 761 1028 2301 Fragments arranged by size: 2301 1473 1028 761 275 186 151 BsiHKAI G_wGCw'C Cuts at: 2679 4236 4321 5392 2679 Size: 1557 85 1071 3462 Fragments arranged by size: 3462 1557 1071 85 BsmI GAATG_Cn' Cuts at: 2670 3686 3779 2670 Size: 1016 93 5066 Fragments arranged by size: 5066 1016 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2679 3494 4236 4321 5392 1391 Size: 399 889 815 742 85 1071 2174 Fragments arranged by size: 2174 1071 889 815 742 399 85 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3530 844 Size: 2686 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2140 4670 4844 65 Size: 1183 892 2530 174 1396 Fragments arranged by size: 2530 1396 1183 892 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6175 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 3210 4235 5247 3210 Size: 1025 1012 4138 Fragments arranged by size: 4138 1025 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstXI CCAn_nnnn'nTGG Cuts at: 2736 2736 Size: 6175 BstYI r'GATC_y Cuts at: 1972 2834 3505 3872 4257 4274 5726 5812 5824 Size: 862 671 367 385 17 1452 86 12 Cuts at: 5824 6170 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 862 671 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4684 Fragments arranged by size: 4684 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 2127 2644 3324 3691 4485 4505 2127 Size: 517 680 367 794 20 3797 Fragments arranged by size: 3797 794 680 517 367 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 2358 3479 2358 Size: 1121 5054 DraIII CAC_nnn'GTG Cuts at: 3212 3212 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140 Size: 54 1425 50 275 18 1792 889 1629 Cuts at: 6140 8 Size: 43 Fragments arranged by size: 1792 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2872 2912 3306 4105 1353 Size: 1519 40 394 799 3423 Fragments arranged by size: 3423 1519 799 394 40 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2432 4762 5136 5282 387 Size: 1064 138 843 2330 374 146 1280 Fragments arranged by size: 2330 1280 1064 843 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2052 3114 3165 4238 4835 5622 5890 Size: 393 420 1062 51 1073 597 787 268 Cuts at: 5890 1239 Size: 1524 Fragments arranged by size: 1524 1073 1062 787 597 420 393 268 51 EcoRI G'AATT_C Cuts at: 3484 3862 3484 Size: 378 5797 EcoRV GAT'ATC Cuts at: 2287 2287 Size: 6175 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2649 3246 2649 Size: 597 5578 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2242 2274 3181 Size: 32 135 32 431 32 207 32 907 Cuts at: 3181 3213 4816 4848 4890 4922 1373 Size: 32 1603 32 42 32 2626 Fragments arranged by size: 2626 1603 907 431 207 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3471 3519 3765 669 Size: 988 281 1533 48 246 3079 Fragments arranged by size: 3079 1533 988 281 246 48 HindIII A'AGCT_T Cuts at: 748 2351 3420 748 Size: 1603 1069 3503 Fragments arranged by size: 3503 1603 1069 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3113 3500 3113 Size: 387 5788 MfeI C'AATT_G Cuts at: 2812 3774 2812 Size: 962 5213 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2641 5289 5473 1305 Size: 1336 2648 184 2007 Fragments arranged by size: 2648 2007 1336 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4133 4492 4651 518 Size: 648 2967 359 159 2042 Fragments arranged by size: 2967 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2072 2095 3106 3591 4268 5416 5661 1606 Size: 466 23 1011 485 677 1148 245 2120 Fragments arranged by size: 2120 1148 1011 677 485 466 245 23 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4684 Fragments arranged by size: 4684 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 3164 3533 5078 1132 Size: 97 1935 369 1545 2229 Fragments arranged by size: 2229 1935 1545 369 97 PasI CC'CwG_GG Cuts at: 1348 3275 1348 Size: 1927 4248 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 3032 3441 1108 Size: 1924 409 3842 Fragments arranged by size: 3842 1924 409 PfoI T'CCnGG_A Cuts at: 2150 2150 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6175 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 2041 3262 3527 830 Size: 1211 1221 265 3478 Fragments arranged by size: 3478 1221 1211 265 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2095 3106 2095 Size: 1011 5164 SalI G'TCGA_C Cuts at: 1936 3469 3517 1936 Size: 1533 48 4594 Fragments arranged by size: 4594 1533 48 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 2037 2988 3258 3523 4658 5339 5530 Size: 219 992 951 270 265 1135 681 191 Cuts at: 5530 826 Size: 1471 Fragments arranged by size: 1471 1135 992 951 681 270 265 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4279 5180 5442 820 Size: 197 926 2336 901 262 1553 Fragments arranged by size: 2336 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2648 3966 493 Size: 2155 1318 2702 Fragments arranged by size: 2702 2155 1318 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 4099 5 Size: 47 4047 2081 Fragments arranged by size: 4047 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2004 3614 513 Size: 552 939 1610 3074 Fragments arranged by size: 3074 1610 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4202 4219 4372 4557 5002 325 Size: 1255 2622 17 153 185 445 1498 Fragments arranged by size: 2622 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4421 6133 96 Size: 274 80 33 51 1443 2444 1712 138 Fragments arranged by size: 2444 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2050 2082 6135 6167 2050 Size: 32 4053 32 2058 Fragments arranged by size: 4053 2058 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 3421 4304 3421 Size: 883 5292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI