################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbsI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstYI CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 831 GTATAC GT^[AC][GT]AC AciI 5 GCGG CCGC(-3/-1) AocI 873 CCTGAGG CC^T[ACGT]AGG Asp700I 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 259 CTCGAG C^[CT]CG[GA]G AvaII 681 GGTCC G^G[AT]CC BanI 607 GGTGCC G^G[CT][GA]CC BanII 896 GGGCTC G[GA]GC[CT]^C BclI 620 TGATCA T^GATCA BfaI 343 CTAG C^TAG BpmI 51 CTCCAG CTGGAG(16/14) BsoBI 259 CTCGAG C^[CT]CG[GA]G BspHI 617 TCATGA T^CATGA BsrBI 3 GAGCGG CCGCTC(-3/-3) BsrDI 765 CATTGC GCAATG(2/0) BsrI 15 CCAGT ACTGG(1/-1) Bst1107I 831 GTATAC GTA^TAC BstZ17I 831 GTATAC GTA^TAC Bsu36I 873 CCTGAGG CC^T[ACGT]AGG Cfr13I 681 GGTCC G^G[ACGT]CC DpnII 621 GATC ^GATC DraII 680 AGGTCCC [GA]G^G[ACGT]CC[CT] EarI 748 CTCTTC CTCTTC(1/4) Eco81I 873 CCTGAGG CC^T[ACGT]AGG EcoO109I 680 AGGTCCC [GA]G^G[ACGT]CC[CT] FauI 5 GCGGG CCCGC(4/6) FbaI 620 TGATCA T^GATCA Ksp632I 748 CTCTTC CTCTTC(1/4) MaeI 343 CTAG C^TAG MboI 621 GATC ^GATC MseI 779 TTAA T^TAA MspA1I 800 CAGCTG C[AC]G^C[GT]G NdeII 621 GATC ^GATC NspI 98 GCATGT [GA]CATG^[CT] PaeR7I 259 CTCGAG C^TCGAG PpuMI 680 AGGTCCC [GA]G^G[AT]CC[CT] PvuII 800 CAGCTG CAG^CTG RcaI 617 TCATGA T^CATGA Sau3AI 621 GATC ^GATC Sau96I 681 GGTCC G^G[ACGT]CC SmlI 259 CTCGAG C^T[CT][GA]AG Tru9I 779 TTAA T^TAA Tsp509I 334 AATT ^AATT XhoI 259 CTCGAG C^TCGAG XmnI 705 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BalI 107 TGGCCA TGG^CCA 245 TGGCCA BbvCI 93 CCTCAGC CCTCAGC(-5/-2) 186 CCTCAGC BspMI 125 ACCTGC ACCTGC(4/8) 664 ACCTGC BstAPI 474 GCACACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 508 GCAGACAATGC BstXI 110 CCACTGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 236 CCAAGATGCTGG Cac8I 161 GCCTGC GC[ACGT]^[ACGT]GC 243 GCTGGC DrdI 9 GACAAACCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 361 GACCACTTTGTC EaeI 107 TGGCCA [CT]^GGCC[GA] 245 TGGCCA EcoNI 579 CCTCAATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 597 CCTTAGTGAGG MaeIII 541 GTGAC ^GT[ACGT]AC 841 GTGAC MluNI 107 TGGCCA TGG^CCA 245 TGGCCA MscI 107 TGGCCA TGG^CCA 245 TGGCCA SfcI 753 CTACAG C^T[GA][CT]AG 867 CTACAG TaqI 19 TCGA T^CGA 260 TCGA Tsp45I 541 GTGAC ^GT[GC]AC 841 GTGAC TspRI 111 CACTG CA[GC]TG[ACGT][ACGT]^ 669 CACTG TthHB8I 19 TCGA T^CGA 260 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AfaI AluI BbvI BmyI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI DdeI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MboII MnlI MslI MvaI MwoI NlaIII NlaIV PleI RsaI ScrFI SfaNI TseI