(Circular) (Six-base) MAPSORT of: pFN21AE9678.seq Check: 7316 from: 1 to: 8956 pFN21AE9678 8956 bp With 182 enzymes: SgfI * March 28, 2012 12:15 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6766 278 Size: 53 83 186 936 5230 2468 Fragments arranged by size: 5230 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5349 5565 6299 1937 Size: 3412 216 734 4594 Fragments arranged by size: 4594 3412 734 216 Acc65I G'GTAC_C Cuts at: 6277 6277 Size: 8956 AclI AA'CG_TT Cuts at: 1563 7083 7456 1563 Size: 5520 373 3063 Fragments arranged by size: 5520 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2115 2206 2757 3191 4559 5826 5901 7019 Size: 483 91 551 434 1368 1267 75 1118 Cuts at: 7019 8403 8671 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1368 1267 1118 551 483 434 268 91 75 AflII C'TTAA_G Cuts at: 820 1017 6233 820 Size: 197 5216 3543 Fragments arranged by size: 5216 3543 197 AflIII A'CryG_T Cuts at: 1203 4722 5308 6092 7855 8832 1203 Size: 3519 586 784 1763 977 1327 Fragments arranged by size: 3519 1763 1327 977 784 586 AgeI A'CCGG_T Cuts at: 7787 7787 Size: 8956 AhdI GACnn_n'nnGTC Cuts at: 3822 7685 3822 Size: 3863 5093 AleI CACnn'nnGTG Cuts at: 1166 3590 1166 Size: 2424 6532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4651 1184 Size: 3467 5489 AlwNI CAG_nnn'CTG Cuts at: 3061 6699 8271 3061 Size: 3638 1572 3746 Fragments arranged by size: 3746 3638 1572 ApaLI G'TGCA_C Cuts at: 7013 8169 7013 Size: 1156 7800 ApoI r'AATT_y Cuts at: 1493 2096 2423 2824 2873 3346 3364 3385 3901 Size: 603 327 401 49 473 18 21 516 Cuts at: 3901 3989 5584 6265 6491 6643 8752 1493 Size: 88 1595 681 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 681 603 516 473 401 327 226 152 88 49 21 18 AseI AT'TA_AT Cuts at: 160 1032 3149 4809 7510 160 Size: 872 2117 1660 2701 1606 Fragments arranged by size: 2701 2117 1660 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8956 AvaI C'yCGr_G Cuts at: 1870 1943 2397 6281 1870 Size: 73 454 3884 4545 Fragments arranged by size: 4545 3884 454 73 AvrII C'CTAG_G Cuts at: 3424 3424 Size: 8956 BamHI G'GATC_C Cuts at: 6137 6286 6653 6137 Size: 149 367 8440 Fragments arranged by size: 8440 367 149 BanI G'GyrC_C Cuts at: 618 943 1160 1787 6277 7732 618 Size: 325 217 627 4490 1455 1842 Fragments arranged by size: 4490 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4743 5949 6275 1391 Size: 3352 1206 326 4072 Fragments arranged by size: 4072 3352 1206 326 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2217 3263 3714 3818 4059 5481 5814 Size: 971 318 1046 451 104 241 1422 333 Cuts at: 5814 5889 928 Size: 75 3995 Fragments arranged by size: 3995 1422 1046 971 451 333 318 241 104 75 BbvCI CC'TCA_GC Cuts at: 3808 5940 3808 Size: 2132 6824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7147 7181 1903 Size: 34 5210 34 3678 Fragments arranged by size: 5210 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2395 6848 8058 2395 Size: 4453 1210 3293 Fragments arranged by size: 4453 3293 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8956 BfrBI ATG'CAT Cuts at: 3718 4895 6248 3718 Size: 1177 1353 6426 Fragments arranged by size: 6426 1353 1177 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7567 136 Size: 107 122 71 631 605 5895 1525 Fragments arranged by size: 5895 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3022 4311 8951 3022 Size: 1289 4640 3027 Fragments arranged by size: 4640 3027 1289 BlpI GC'TnA_GC Cuts at: 6373 6373 Size: 8956 Bme1580I G_kGCm'C Cuts at: 1790 7017 8173 1790 Size: 5227 1156 2573 Fragments arranged by size: 5227 2573 1156 BmgBI CAC'GTC Cuts at: 1318 4876 1318 Size: 3558 5398 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3526 3712 6687 Size: 416 53 83 399 24 2109 186 2975 Cuts at: 6687 7645 442 Size: 958 1753 Fragments arranged by size: 2975 2109 1753 958 416 399 186 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8956 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4757 4757 Size: 8956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2268 2901 4098 4241 4287 4364 4678 6109 Size: 1029 633 1197 143 46 77 314 1431 Cuts at: 6109 7616 1239 Size: 1507 2579 Fragments arranged by size: 2579 1507 1431 1197 1029 633 314 143 77 46 Bpu10I CC'TnA_GC Cuts at: 2760 3808 5940 2760 Size: 1048 2132 5776 Fragments arranged by size: 5776 2132 1048 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2396 4171 5726 6147 7081 7946 8244 2396 Size: 1775 1555 421 934 865 298 3108 Fragments arranged by size: 3108 1775 1555 934 865 421 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7619 882 Size: 617 6120 2219 Fragments arranged by size: 6120 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6747 493 Size: 711 5543 2702 Fragments arranged by size: 5543 2702 711 BsaBI GATnn'nnATC Cuts at: 2317 2714 8950 2317 Size: 397 6236 2323 Fragments arranged by size: 6236 2323 397 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6763 7145 7780 275 Size: 53 83 186 936 5230 382 635 1451 Fragments arranged by size: 5230 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4477 4870 7389 7787 8061 8208 8619 4477 Size: 393 2519 398 274 147 411 4814 Fragments arranged by size: 4814 2519 411 398 393 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4676 4706 5356 5386 1178 Size: 30 165 30 3273 30 650 30 4748 Fragments arranged by size: 4748 3273 650 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2629 5957 6058 1186 Size: 1443 3328 101 4084 Fragments arranged by size: 4084 3328 1443 101 BseYI C'CCAG_C Cuts at: 8159 8655 8159 Size: 496 8460 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4023 4814 1163 Size: 573 2287 791 5305 Fragments arranged by size: 5305 2287 791 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6406 7167 8195 1540 Size: 275 186 4405 761 1028 2301 Fragments arranged by size: 4405 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3138 5949 7017 7102 8173 3138 Size: 2811 1068 85 1071 3921 Fragments arranged by size: 3921 2811 1071 1068 85 BsmI GAATG_Cn' Cuts at: 5114 6248 6467 6560 5114 Size: 1134 219 93 7510 Fragments arranged by size: 7510 1134 219 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3138 4743 5949 6275 7017 7102 8173 Size: 399 1348 1605 1206 326 742 85 1071 Cuts at: 8173 1391 Size: 2174 Fragments arranged by size: 2174 1605 1348 1206 1071 742 399 326 85 BspEI T'CCGG_A Cuts at: 8619 8619 Size: 8956 BspHI T'CATG_A Cuts at: 4990 6845 8575 4990 Size: 1855 1730 5371 Fragments arranged by size: 5371 1855 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 6311 844 Size: 5467 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6843 1394 Size: 429 5020 3507 Fragments arranged by size: 5020 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2730 7451 7625 65 Size: 1183 1482 4721 174 1396 Fragments arranged by size: 4721 1482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7600 7787 1700 Size: 110 5790 187 2869 Fragments arranged by size: 5790 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8956 BssSI C'ACGA_G Cuts at: 7016 8028 7016 Size: 1012 7944 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8956 BstBI TT'CG_AA Cuts at: 7760 7760 Size: 8956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8956 BstXI CCAn_nnnn'nTGG Cuts at: 4934 4934 Size: 8956 BstYI r'GATC_y Cuts at: 1972 3022 4311 5405 6137 6286 6653 7038 7055 Size: 1050 1289 1094 732 149 367 385 17 Cuts at: 7055 8507 8593 8605 8951 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1289 1094 1050 732 385 367 346 149 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4554 5775 4554 Size: 1221 7735 BtgI C'CryG_G Cuts at: 513 1065 2004 4713 513 Size: 552 939 2709 4756 Fragments arranged by size: 4756 2709 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2718 5856 487 Size: 683 56 1492 3138 3587 Fragments arranged by size: 3587 3138 1492 683 56 BtsI GCAGTG_nn' Cuts at: 2547 3585 4040 4966 5599 6472 7266 7286 2547 Size: 1038 455 926 633 873 794 20 4217 Fragments arranged by size: 4217 1038 926 873 794 633 455 20 ClaI AT'CG_AT Cuts at: 1554 2718 1554 Size: 1164 7792 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8921 DraI TTT'AAA Cuts at: 2116 2275 2587 5443 5559 6260 2116 Size: 159 312 2856 116 701 4812 Fragments arranged by size: 4812 2856 701 312 159 116 DraIII CAC_nnn'GTG Cuts at: 4400 4400 Size: 8956 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7963 809 Size: 507 6647 1802 Fragments arranged by size: 6647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3776 4494 6403 Size: 54 1425 50 275 18 1946 718 1909 Cuts at: 6403 7292 8921 8 Size: 889 1629 43 Fragments arranged by size: 1946 1909 1629 1425 889 718 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6403 1537 Size: 275 4591 4090 Fragments arranged by size: 4591 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2953 4942 5634 6002 6886 1353 Size: 1600 1989 692 368 884 3423 Fragments arranged by size: 3423 1989 1600 884 692 368 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7543 7917 8063 387 Size: 1064 138 5954 374 146 1280 Fragments arranged by size: 5954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 5947 5947 Size: 8956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2115 2206 2268 2757 2901 3191 4098 Size: 393 483 91 62 489 144 290 907 Cuts at: 4098 4241 4287 4364 4559 4678 5826 5901 6109 Size: 143 46 77 195 119 1148 75 208 Cuts at: 6109 7019 7616 8403 8671 1239 Size: 910 597 787 268 1524 Fragments arranged by size: 1524 1148 910 907 787 597 489 483 393 290 268 208 195 144 143 119 91 77 75 62 46 EcoNI CCTnn'n_nnAGG Cuts at: 6031 6031 Size: 8956 EcoO109I rG'GnC_Cy Cuts at: 2974 5315 5921 2974 Size: 2341 606 6009 Fragments arranged by size: 6009 2341 606 EcoRI G'AATT_C Cuts at: 3346 6265 6643 3346 Size: 2919 378 5659 Fragments arranged by size: 5659 2919 378 EcoRV GAT'ATC Cuts at: 6105 6105 Size: 8956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2287 2596 2612 5584 2287 Size: 309 16 2972 5659 Fragments arranged by size: 5659 2972 309 16 FspI TGC'GCA Cuts at: 7462 7462 Size: 8956 HaeII r_GCGC'y Cuts at: 1678 8103 1678 Size: 6425 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3014 3046 3808 3840 4146 Size: 32 135 32 1442 32 762 32 306 Cuts at: 4146 4178 6187 6219 7597 7629 7671 7703 1373 Size: 32 2009 32 1378 32 42 32 2626 Fragments arranged by size: 2626 2009 1442 1378 762 306 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5350 6300 6546 669 Size: 988 281 3412 950 246 3079 Fragments arranged by size: 3412 3079 988 950 281 246 HindIII A'AGCT_T Cuts at: 748 5747 748 Size: 4999 3957 HpaI GTT'AAC Cuts at: 6546 6546 Size: 8956 KpnI G_GTAC'C Cuts at: 6281 6281 Size: 8956 MfeI C'AATT_G Cuts at: 4842 6555 4842 Size: 1713 7243 MluI A'CGCG_T Cuts at: 8832 8832 Size: 8956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8070 8254 1305 Size: 6765 184 2007 Fragments arranged by size: 6765 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 7134 Fragments arranged by size: 7134 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2748 3590 4665 5091 5181 6914 7273 Size: 648 1582 842 1075 426 90 1733 359 Cuts at: 7273 7432 518 Size: 159 2042 Fragments arranged by size: 2042 1733 1582 1075 842 648 426 359 159 90 MspA1I CmG'CkG Cuts at: 1606 3214 3749 4537 5879 5939 6372 7049 8197 Size: 1608 535 788 1342 60 433 677 1148 Cuts at: 8197 8442 1606 Size: 245 2120 Fragments arranged by size: 2120 1608 1342 1148 788 677 535 433 245 60 NcoI C'CATG_G Cuts at: 513 1065 2004 4713 513 Size: 552 939 2709 4756 Fragments arranged by size: 4756 2709 939 552 NdeI CA'TA_TG Cuts at: 387 4666 6244 387 Size: 4279 1578 3099 Fragments arranged by size: 4279 3099 1578 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8956 NotI GC'GGCC_GC Cuts at: 6403 6403 Size: 8956 NsiI A_TGCA'T Cuts at: 3720 4897 6250 3720 Size: 1177 1353 6426 Fragments arranged by size: 6426 1353 1177 NspI r_CATG'y Cuts at: 1132 1229 5312 6096 6314 7859 1132 Size: 97 4083 784 218 1545 2229 Fragments arranged by size: 4083 2229 1545 784 218 97 PasI CC'CwG_GG Cuts at: 1348 4509 5894 1348 Size: 3161 1385 4410 Fragments arranged by size: 4410 3161 1385 PciI A'CATG_T Cuts at: 5308 6092 7855 5308 Size: 784 1763 6409 Fragments arranged by size: 6409 1763 784 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8956 PmeI GTTT'AAAC Cuts at: 6260 6260 Size: 8956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7025 7057 8538 8570 7025 Size: 32 1481 32 7411 Fragments arranged by size: 7411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2974 5315 2974 Size: 2341 6615 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8956 PsiI TTA'TAA Cuts at: 5018 5220 6526 5018 Size: 202 1306 7448 Fragments arranged by size: 7448 1306 202 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 5250 5282 5250 Size: 32 8924 PstI C_TGCA'G Cuts at: 830 6308 830 Size: 5478 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8956 PvuII CAG'CTG Cuts at: 3749 4537 5879 3749 Size: 788 1342 6826 Fragments arranged by size: 6826 1342 788 SacI G_AGCT'C Cuts at: 5949 5949 Size: 8956 SalI G'TCGA_C Cuts at: 1936 5348 6298 1936 Size: 3412 950 4594 Fragments arranged by size: 4594 3412 950 SbfI CC_TGCA'GG Cuts at: 6308 6308 Size: 8956 ScaI AGT'ACT Cuts at: 7204 8916 7204 Size: 1712 7244 SfcI C'TryA_G Cuts at: 826 1045 2030 3339 6304 7439 8120 8311 826 Size: 219 985 1309 2965 1135 681 191 1471 Fragments arranged by size: 2965 1471 1309 1135 985 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8956 SmaI CCC'GGG Cuts at: 1872 2399 6283 1872 Size: 527 3884 4545 Fragments arranged by size: 4545 3884 527 SmlI C'TyrA_G Cuts at: 820 1017 1943 2411 4150 5741 6162 6233 7060 Size: 197 926 468 1739 1591 421 71 827 Cuts at: 7060 7961 8223 820 Size: 901 262 1553 Fragments arranged by size: 1739 1591 1553 926 901 827 468 421 262 197 71 SnaBI TAC'GTA Cuts at: 493 6747 493 Size: 6254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8956 SphI G_CATG'C Cuts at: 1132 6314 1132 Size: 5182 3774 SspI AAT'ATT Cuts at: 5 52 4249 5722 6880 5 Size: 47 4197 1473 1158 2081 Fragments arranged by size: 4197 2081 1473 1158 47 StuI AGG'CCT Cuts at: 1517 3298 3472 1517 Size: 1781 174 7001 Fragments arranged by size: 7001 1781 174 StyI C'CwwG_G Cuts at: 513 1065 2004 3424 4191 4713 6069 6395 513 Size: 552 939 1420 767 522 1356 326 3074 Fragments arranged by size: 3074 1420 1356 939 767 552 522 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4677 5193 6983 7000 7153 7338 7783 Size: 1255 3097 516 1790 17 153 185 445 Cuts at: 7783 325 Size: 1498 Fragments arranged by size: 3097 1790 1498 1255 516 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5542 7202 8914 Size: 274 80 33 51 1443 3565 1660 1712 Cuts at: 8914 96 Size: 138 Fragments arranged by size: 3565 1712 1660 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4489 4521 5356 5388 8916 8948 4489 Size: 32 835 32 3528 32 4497 Fragments arranged by size: 4497 3528 835 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8956 XbaI T'CTAG_A Cuts at: 6292 6292 Size: 8956 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6187 6187 Size: 8956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8956 XmaI C'CCGG_G Cuts at: 1870 2397 6281 1870 Size: 527 3884 4545 Fragments arranged by size: 4545 3884 527 XmnI GAAnn'nnTTC Cuts at: 3204 7085 3204 Size: 3881 5075 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6764 276 Size: 53 83 186 936 5230 2468 Fragments arranged by size: 5230 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI