################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BamHI BbrPI BbvCI BcgI BcnI BfrI BlnI BpmI BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI BspEI BspLU11I BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I Cfr10I CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HindIII HpaI KpnI MfeI MluI MroI MunI NaeI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcyI 713 GGCGCC G[GA]^CG[CT]C AflIII 164 ACGTGT A^C[GA][CT]GT Alw44I 982 GTGCAC G^TGCAC AlwNI 95 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 982 GTGCAC G^TGCAC Asp700I 1140 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 172 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbeI 713 GGCGCC GGCGC^C BciVI 446 GGATAC GTATCC(6/5) BclI 866 TGATCA T^GATCA BlpI 100 GCTGAGC GC^T[ACGT]AGC Bpu1102I 100 GCTGAGC GC^T[ACGT]AGC BsaBI 1227 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 713 GGCGCC G[GA]^CG[CT]C BsgI 335 CTGCAC GTGCAG(16/14) BsmI 1460 GCATTC GAATGC(1/-1) Bsp1407I 704 TGTACA T^GTACA BspDI 559 ATCGAT AT^CGAT BspHI 1064 TCATGA T^CATGA BsrGI 704 TGTACA T^GTACA BssSI 1257 CACGAG CACGAG(-5/-1) BstEII 816 GGTGACC G^GT[ACGT]ACC BstPI 816 GGTGACC G^GT[ACGT]ACC BstXI 887 CCACCTCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 100 GCTGAGC GC^T[ACGT]AGC ClaI 559 ATCGAT AT^CGAT EarI 579 CTCTTC CTCTTC(1/4) EcoO65I 816 GGTGACC G^GT[ACGT]ACC EcoT14I 1415 CCATGG C^C[AT][AT]GG FauI 12 CCCGC CCCGC(4/6) FbaI 866 TGATCA T^GATCA Hin1I 713 GGCGCC G[GA]^CG[CT]C KasI 713 GGCGCC G^GCGCC Ksp632I 579 CTCTTC CTCTTC(1/4) KspI 43 CCGCGG CCGC^GG MamI 1227 GATAAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 464 TTAA T^TAA NarI 713 GGCGCC GG^CGCC NcoI 1415 CCATGG C^CATGG PflFI 172 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 1159 GAGTC GAGTC(4/5) PvuII 208 CAGCTG CAG^CTG RcaI 1064 TCATGA T^CATGA SacII 43 CCGCGG CCGC^GG SapI 578 GCTCTTC GCTCTTC(1/4) SfoI 713 GGCGCC GGC^GCC SspBI 704 TGTACA T^GTACA StyI 1415 CCATGG C^C[AT][AT]GG Tru9I 464 TTAA T^TAA Tth111I 172 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 200 CCATGTCTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1140 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 44 CGCG CG^CG 1153 CGCG AvaII 539 GGTCC G^G[AT]CC 567 GGTCC BalI 83 TGGCCA TGG^CCA 1499 TGGCCA BanI 713 GGCGCC G^G[CT][GA]CC 1120 GGTGCC BfaI 1268 CTAG C^TAG 1465 CTAG BglI 39 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 91 GCCTCAGAGGC BglII 344 AGATCT A^GATCT 677 AGATCT BsaI 244 GAGACC GGTCTC(1/5) 874 GAGACC BseRI 838 CTCCTC GAGGAG(10/8) 673 GAGGAG BspMI 791 ACCTGC ACCTGC(4/8) 1043 ACCTGC BstUI 44 CGCG CG^CG 1153 CGCG BstYI 344 AGATCT [GA]^GATC[CT] 677 AGATCT DraII 88 AGGGCCT [GA]G^G[ACGT]CC[CT] 1108 AGGCCCC DsaI 43 CCGCGG C^C[GA][CT]GG 1415 CCATGG EcoO109I 88 AGGGCCT [GA]G^G[ACGT]CC[CT] 1108 AGGCCCC HaeII 30 GGCGCT [GA]GCGC^[CT] 713 GGCGCC MaeI 1268 CTAG C^TAG 1465 CTAG MflI 344 AGATCT [GA]^GATC[CT] 677 AGATCT MluNI 83 TGGCCA TGG^CCA 1499 TGGCCA MscI 83 TGGCCA TGG^CCA 1499 TGGCCA MslI 1059 CATGTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1449 CACCCTGGTG MspA1I 43 CCGCGG C[AC]G^C[GT]G 208 CAGCTG MvnI 44 CGCG CG^CG 1153 CGCG PflMI 234 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 248 CCAACCTGTGG SacI 468 GAGCTC GAGCT^C 1126 GAGCTC SfaNI 1285 GATGC GCATC(5/9) 1510 GATGC SfcI 155 CTGTAG C^T[GA][CT]AG 987 CTACAG TfiI 1276 GAATC G^A[AT]TC 1389 GATTC Tsp509I 1141 AATT ^AATT 1240 AATT Van91I 234 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 248 CCAACCTGTGG XhoII 344 AGATCT [GA]^GATC[CT] 677 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AspHI BanII BbsI BbvI BmyI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmlI TaqI TseI Tsp45I TspRI TthHB8I