################################## # NO CUT ENZYMES ( 133 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflIII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII BalI BbvCI BcgI BciVI BclI BglII BlpI Bpu1102I BsaBI BsaI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KpnI KspI MamI MfeI MluI MluNI MscI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfaNI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccIII 137 TCCGGA T^CCGGA AflII 385 CTTAAG C^TTAAG AluI 531 AGCT AG^CT Alw44I 106 GTGCAC G^TGCAC AlwNI 201 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 106 GTGCAC G^TGCAC AspHI 106 GTGCAC G[AT]GC[AT]^C AvrII 516 CCTAGG C^CTAGG BamHI 432 GGATCC G^GATCC BanII 69 GGGCTC G[GA]GC[CT]^C BbrPI 234 CACGTG CAC^GTG BbsI 196 GTCTTC GAAGAC(2/6) BfrI 385 CTTAAG C^TTAAG BlnI 516 CCTAGG C^CTAGG BpmI 533 CTGGAG CTGGAG(16/14) BpuAI 196 GTCTTC GAAGAC(2/6) BsaAI 234 CACGTG [CT]AC^GT[GA] BseAI 137 TCCGGA T^CCGGA BseRI 510 GAGGAG GAGGAG(10/8) BsgI 271 CTGCAC GTGCAG(16/14) BsiHKAI 106 GTGCAC G[AT]GC[AT]^C BsiWI 303 CGTACG C^GTACG BsmAI 374 GTCTC GTCTC(1/5) BspEI 137 TCCGGA T^CCGGA BsrFI 363 GCCGGC [GA]^CCGG[CT] BssHII 186 GCGCGC G^CGCGC Cfr10I 363 GCCGGC [GA]^CCGG[CT] DdeI 376 CTCAG C^T[ACGT]AG EarI 28 GAAGAG CTCTTC(1/4) EcoNI 270 CCTGCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HinfI 462 GAATC G^A[ACGT]TC Ksp632I 28 GAAGAG CTCTTC(1/4) MroI 137 TCCGGA T^CCGGA MseI 386 TTAA T^TAA NaeI 363 GCCGGC GCC^GGC NgoMIV 363 GCCGGC G^CCGGC NspI 110 ACATGC [GA]CATG^[CT] PmaCI 234 CACGTG CAC^GTG PmlI 234 CACGTG CAC^GTG PvuII 530 CAGCTG CAG^CTG SexAI 275 ACCAGGT A^CC[AT]GGT SmaI 66 CCCGGG CCC^GGG TfiI 462 GAATC G^A[AT]TC Tru9I 386 TTAA T^TAA XmaCI 66 CCCGGG C^CCGGG XmaI 66 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AfaI 304 GTAC GT^AC 382 GTAC AvaI 66 CCCGGG C^[CT]CG[GA]G 562 CCCGAG AvaII 335 GGTCC G^G[AT]CC 475 GGTCC BanI 33 GGCGCC G^G[CT][GA]CC 93 GGCGCC BbeI 33 GGCGCC GGCGC^C 93 GGCGCC BfaI 371 CTAG C^TAG 517 CTAG BglI 163 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 260 GCCTGGCGGGC BsaWI 137 TCCGGA [AT]^CCGG[AT] 296 ACCGGA BsiEI 210 CGGCCG CG[GA][CT]^CG 365 CGGCCG BsmFI 476 GTCCC GGGAC(10/14) 483 GTCCC BsoBI 66 CCCGGG C^[CT]CG[GA]G 562 CCCGAG BstF5I 408 CATCC GGATG(2/0) 469 CATCC EaeI 210 CGGCCG [CT]^GGCC[GA] 365 CGGCCG EagI 210 CGGCCG C^GGCCG 365 CGGCCG EclXI 210 CGGCCG C^GGCCG 365 CGGCCG Eco52I 210 CGGCCG C^GGCCG 365 CGGCCG EcoT14I 419 CCAAGG C^C[AT][AT]GG 516 CCTAGG FokI 408 CATCC GGATG(9/13) 469 CATCC KasI 33 GGCGCC G^GCGCC 93 GGCGCC MaeI 371 CTAG C^TAG 517 CTAG MaeII 235 ACGT A^CGT 392 ACGT MaeIII 232 GTCAC ^GT[ACGT]AC 280 GTCAC MboII 28 GAAGA GAAGA(8/7) 197 TCTTC NarI 33 GGCGCC GG^CGCC 93 GGCGCC NlaIII 111 CATG CATG^ 168 CATG RsaI 304 GTAC GT^AC 382 GTAC SfoI 33 GGCGCC GGC^GCC 93 GGCGCC SmlI 250 CTCAAG C^T[CT][GA]AG 385 CTTAAG StyI 419 CCAAGG C^C[AT][AT]GG 516 CCTAGG Tsp45I 232 GTCAC ^GT[GC]AC 280 GTCAC XcmI 158 CCACCGCCAGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 419 CCAAGGACGCGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AccII AciI AcyI AlwI BbvI BcnI BmyI BsaHI BsaJI BsiYI BslI Bsp1286I BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DpnII EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII ItaI MboI MflI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIV Sau3AI Sau96I ScrFI TseI XhoII