Restriction map for
pFN21AE5660
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HindIII HpaI KasI KspI
MamI MluI MroI MslI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 424 GTCGAC GT^[AC][GT]AC
AccII 1258 CGCG CG^CG
AcsI 188 AAATTC [GA]^AATT[CT]
AlwNI 203 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 188 AAATTC [GA]^AATT[CT]
Asp700I 1245 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1213 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 698 CCCGGG C^[CT]CG[GA]G
BalI 543 TGGCCA TGG^CCA
BglI 272 GCCAAGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1191 AGATCT A^GATCT
BsaI 230 GAGACC GGTCTC(1/5)
BsmFI 835 GTCCC GGGAC(10/14)
BsmI 1439 GCATTC GAATGC(1/-1)
BsoBI 698 CCCGGG C^[CT]CG[GA]G
BsrBI 904 GAGCGG CCGCTC(-3/-3)
BstUI 1258 CGCG CG^CG
CfoI 1257 GCGC GCG^C
DraI 534 TTTAAA TTT^AAA
DraIII 793 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 875 CCGTGG C^C[GA][CT]GG
EaeI 543 TGGCCA [CT]^GGCC[GA]
EcoNI 671 CCTATGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1164 CCAAGG C^C[AT][AT]GG
FauI 393 CCCGC CCCGC(4/6)
HhaI 1257 GCGC GCG^C
HinP1I 1257 GCGC G^CGC
HincII 424 GTCGAC GT[CT]^[GA]AC
HindII 424 GTCGAC GT[CT]^[GA]AC
MfeI 36 CAATTG C^AATTG
MluNI 543 TGGCCA TGG^CCA
MscI 543 TGGCCA TGG^CCA
MunI 36 CAATTG C^AATTG
MvnI 1258 CGCG CG^CG
PflFI 1213 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
SalI 424 GTCGAC G^TCGAC
ScaI 1314 AGTACT AGT^ACT
SexAI 1214 ACCTGGT A^CC[AT]GGT
SmaI 698 CCCGGG CCC^GGG
StuI 1000 AGGCCT AGG^CCT
StyI 1164 CCAAGG C^C[AT][AT]GG
Tth111I 1213 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 698 CCCGGG C^CCGGG
XmaI 698 CCCGGG C^CCGGG
XmnI 1245 GAACGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
Acc65I 688 GGTACC G^GTACC
728 GGTACC
Asp718I 688 GGTACC G^GTACC
728 GGTACC
AspHI 408 GTGCTC G[AT]GC[AT]^C
1405 GAGCAC
BanII 760 GAGCCC G[GA]GC[CT]^C
1040 GGGCTC
BbsI 1249 GTCTTC GAAGAC(2/6)
1453 GTCTTC
BpuAI 1249 GTCTTC GAAGAC(2/6)
1453 GTCTTC
BsgI 813 CTGCAC GTGCAG(16/14)
1513 CTGCAC
BsiHKAI 408 GTGCTC G[AT]GC[AT]^C
1405 GAGCAC
BspMI 166 GCAGGT ACCTGC(4/8)
1449 GCAGGT
BstXI 219 CCAACATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
935 CCAGTCAGATGG
BstYI 593 AGATCC [GA]^GATC[CT]
1191 AGATCT
KpnI 688 GGTACC GGTAC^C
728 GGTACC
MaeII 797 ACGT A^CGT
1247 ACGT
MflI 593 AGATCC [GA]^GATC[CT]
1191 AGATCT
MseI 184 TTAA T^TAA
535 TTAA
PflMI 202 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1458 CCAGGAGGTGG
PpuMI 204 AGGACCT [GA]G^G[AT]CC[CT]
963 AGGACCT
SapI 369 GAAGAGC GCTCTTC(1/4)
1149 GCTCTTC
SfcI 5 CTACAG C^T[GA][CT]AG
1004 CTACAG
SmlI 444 CTTGAG C^T[CT][GA]AG
901 CTTGAG
Tru9I 184 TTAA T^TAA
535 TTAA
Van91I 202 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1458 CCAGGAGGTGG
XhoII 593 AGATCC [GA]^GATC[CT]
1191 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanI BbvI BcnI BfaI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI