(Circular) (Six-base) MAPSORT of: pFN21AE5660.seq Check: 478 from: 1 to: 6229 pFN21AE5660 6229 bp With 182 enzymes: SgfI * December 8, 2014 13:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2162 2162 Size: 6229 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4039 278 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2430 3572 1937 Size: 493 1142 4594 Fragments arranged by size: 4594 1142 493 Acc65I G'GTAC_C Cuts at: 2693 2733 3550 2693 Size: 40 817 5372 Fragments arranged by size: 5372 817 40 AclI AA'CG_TT Cuts at: 1563 4356 4729 1563 Size: 2793 373 3063 Fragments arranged by size: 3063 2793 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2149 3142 4292 5676 5944 1632 Size: 517 993 1150 1384 268 1917 Fragments arranged by size: 1917 1384 1150 993 517 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6032 AflIII A'CryG_T Cuts at: 1203 5128 6105 1203 Size: 3925 977 1327 Fragments arranged by size: 3925 1327 977 AgeI A'CCGG_T Cuts at: 5060 5060 Size: 6229 AhdI GACnn_n'nnGTC Cuts at: 4958 4958 Size: 6229 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6229 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6229 AlwNI CAG_nnn'CTG Cuts at: 2213 3972 5544 2213 Size: 1759 1572 2898 Fragments arranged by size: 2898 1759 1572 ApaLI G'TGCA_C Cuts at: 4286 5442 4286 Size: 1156 5073 ApoI r'AATT_y Cuts at: 1493 2193 3538 3764 3916 6025 1493 Size: 700 1345 226 152 2109 1697 Fragments arranged by size: 2109 1697 1345 700 226 152 AseI AT'TA_AT Cuts at: 160 1032 4783 160 Size: 872 3751 1606 Fragments arranged by size: 3751 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 AvaI C'yCGr_G Cuts at: 1870 1943 2703 3554 1870 Size: 73 760 851 4545 Fragments arranged by size: 4545 851 760 73 BamHI G'GATC_C Cuts at: 3559 3926 3559 Size: 367 5862 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2505 2693 2733 3069 3313 Size: 325 217 627 718 188 40 336 244 Cuts at: 3313 3550 5005 618 Size: 237 1455 1842 Fragments arranged by size: 1842 1455 718 627 336 325 244 237 217 188 40 BanII G_rGCy'C Cuts at: 1391 2769 3049 3548 1391 Size: 1378 280 499 4072 Fragments arranged by size: 4072 1378 499 280 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3247 3451 928 Size: 971 1348 204 3706 Fragments arranged by size: 3706 1348 971 204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4420 4454 1903 Size: 34 2483 34 3678 Fragments arranged by size: 3678 2483 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4121 5331 4121 Size: 1210 5019 BclI T'GATC_A Cuts at: 1548 1548 Size: 6229 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2283 4840 136 Size: 107 122 71 631 605 611 2557 1525 Fragments arranged by size: 2557 1525 631 611 605 122 107 71 BglII A'GATC_T Cuts at: 3196 6224 3196 Size: 3028 3201 BlpI GC'TnA_GC Cuts at: 3646 3646 Size: 6229 Bme1580I G_kGCm'C Cuts at: 1790 2344 2796 3316 4290 5446 1790 Size: 554 452 520 974 1156 2573 Fragments arranged by size: 2573 1156 974 554 520 452 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6229 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3395 3413 3960 Size: 416 53 83 399 24 1978 18 547 Cuts at: 3960 4918 442 Size: 958 1753 Fragments arranged by size: 1978 1753 958 547 416 399 83 53 24 18 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6229 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 2235 2939 4889 1239 Size: 980 16 704 1950 2579 Fragments arranged by size: 2579 1950 980 704 16 Bpu10I CC'TnA_GC Cuts at: 3048 3048 Size: 6229 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2470 2927 4354 5219 5517 2470 Size: 457 1427 865 298 3182 Fragments arranged by size: 3182 1427 865 457 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2229 4892 882 Size: 617 730 2663 2219 Fragments arranged by size: 2663 2219 730 617 BsaAI yAC'GTr Cuts at: 493 1204 4020 493 Size: 711 2816 2702 Fragments arranged by size: 2816 2702 711 BsaBI GATnn'nnATC Cuts at: 6223 6223 Size: 6229 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4036 4418 5053 275 Size: 53 83 186 936 2503 382 635 1451 Fragments arranged by size: 2503 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4662 5060 5334 5481 5892 4662 Size: 398 274 147 411 4999 Fragments arranged by size: 4999 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2677 2707 2943 2973 1178 Size: 30 165 30 1274 30 236 30 4434 Fragments arranged by size: 4434 1274 236 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 2678 2951 1186 Size: 1423 69 273 4464 Fragments arranged by size: 4464 1423 273 69 BseYI C'CCAG_C Cuts at: 2020 3450 5432 5928 2020 Size: 1430 1982 496 2321 Fragments arranged by size: 2321 1982 1430 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2803 3503 1163 Size: 573 1067 700 3889 Fragments arranged by size: 3889 1067 700 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 4440 5468 1540 Size: 275 186 1678 761 1028 2301 Fragments arranged by size: 2301 1678 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2417 3414 4290 4375 5446 2417 Size: 997 876 85 1071 3200 Fragments arranged by size: 3200 1071 997 876 85 BsmI GAATG_Cn' Cuts at: 3444 3740 3833 3444 Size: 296 93 5840 Fragments arranged by size: 5840 296 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2344 2417 2769 2796 3049 3316 3414 Size: 399 554 73 352 27 253 267 98 Cuts at: 3414 3548 4290 4375 5446 1391 Size: 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 352 267 253 134 98 85 73 27 BspEI T'CCGG_A Cuts at: 5892 5892 Size: 6229 BspHI T'CATG_A Cuts at: 4118 5848 4118 Size: 1730 4499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2162 3445 3584 844 Size: 1318 1283 139 3489 Fragments arranged by size: 3489 1318 1283 139 BsrBI CCG'CTC Cuts at: 1394 1823 2911 4116 1394 Size: 429 1088 1205 3507 Fragments arranged by size: 3507 1205 1088 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4724 4898 65 Size: 1183 3476 174 1396 Fragments arranged by size: 3476 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4873 5060 1700 Size: 110 3063 187 2869 Fragments arranged by size: 3063 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6229 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6229 BssSI C'ACGA_G Cuts at: 4289 5301 4289 Size: 1012 5217 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6229 BstBI TT'CG_AA Cuts at: 5033 5033 Size: 6229 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6229 BstXI CCAn_nnnn'nTGG Cuts at: 2231 2947 2231 Size: 716 5513 BstYI r'GATC_y Cuts at: 1972 2598 3196 3559 3926 4311 4328 5780 5866 Size: 626 598 363 367 385 17 1452 86 Cuts at: 5866 5878 6224 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 626 598 385 367 363 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2880 513 Size: 552 939 876 3862 Fragments arranged by size: 3862 939 876 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5490 Fragments arranged by size: 5490 683 56 BtsI GCAGTG_nn' Cuts at: 2344 3515 3745 4539 4559 2344 Size: 1171 230 794 20 4014 Fragments arranged by size: 4014 1171 794 230 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6229 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2515 2550 529 Size: 35 1951 35 4208 Fragments arranged by size: 4208 1951 35 35 DraI TTT'AAA Cuts at: 2541 3533 2541 Size: 992 5237 DraIII CAC_nnn'GTG Cuts at: 2803 2803 Size: 6229 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5236 809 Size: 507 3920 1802 Fragments arranged by size: 3920 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2548 3676 4565 Size: 54 1425 50 275 18 718 1128 889 Cuts at: 4565 6194 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1128 889 718 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 1537 Size: 275 1864 4090 Fragments arranged by size: 4090 1864 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2068 2335 2369 3161 4159 1353 Size: 715 267 34 792 998 3423 Fragments arranged by size: 3423 998 792 715 267 34 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4816 5190 5336 387 Size: 1064 138 3227 374 146 1280 Fragments arranged by size: 3227 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2149 2219 2235 2939 3142 4292 4889 Size: 393 517 70 16 704 203 1150 597 Cuts at: 4889 5676 5944 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1150 787 704 597 517 393 268 203 70 16 EcoNI CCTnn'n_nnAGG Cuts at: 2680 2680 Size: 6229 EcoO109I rG'GnC_Cy Cuts at: 2210 2258 2969 2210 Size: 48 711 5470 Fragments arranged by size: 5470 711 48 EcoRI G'AATT_C Cuts at: 3538 3916 3538 Size: 378 5851 FspI TGC'GCA Cuts at: 4735 4735 Size: 6229 HaeII r_GCGC'y Cuts at: 1678 5376 1678 Size: 3698 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2095 2127 4870 4902 4944 Size: 32 135 32 523 32 2743 32 42 Cuts at: 4944 4976 1373 Size: 32 2626 Fragments arranged by size: 2743 2626 523 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2431 3573 3819 669 Size: 988 281 493 1142 246 3079 Fragments arranged by size: 3079 1142 988 493 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6229 HpaI GTT'AAC Cuts at: 3819 3819 Size: 6229 KpnI G_GTAC'C Cuts at: 2697 2737 3554 2697 Size: 40 817 5372 Fragments arranged by size: 5372 817 40 MfeI C'AATT_G Cuts at: 2041 3828 2041 Size: 1787 4442 MluI A'CGCG_T Cuts at: 6105 6105 Size: 6229 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3108 5343 5527 1305 Size: 1803 2235 184 2007 Fragments arranged by size: 2235 2007 1803 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2550 10 Size: 54 1425 343 718 3689 Fragments arranged by size: 3689 1425 718 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4187 4546 4705 518 Size: 648 3021 359 159 2042 Fragments arranged by size: 3021 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2084 2654 3060 3287 3645 4322 5470 5715 Size: 478 570 406 227 358 677 1148 245 Cuts at: 5715 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 570 478 406 358 245 227 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4738 Fragments arranged by size: 4738 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6229 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6229 NotI GC'GGCC_GC Cuts at: 3676 3676 Size: 6229 NspI r_CATG'y Cuts at: 1132 1229 3587 5132 1132 Size: 97 2358 1545 2229 Fragments arranged by size: 2358 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6229 PciI A'CATG_T Cuts at: 5128 5128 Size: 6229 PflMI CCAn_nnn'nTGG Cuts at: 1108 2213 3469 1108 Size: 1105 1256 3868 Fragments arranged by size: 3868 1256 1105 PfoI T'CCnGG_A Cuts at: 3462 3462 Size: 6229 PmeI GTTT'AAAC Cuts at: 3533 3533 Size: 6229 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4298 4330 5811 5843 4298 Size: 32 1481 32 4684 Fragments arranged by size: 4684 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2210 2969 2210 Size: 759 5470 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6229 PsiI TTA'TAA Cuts at: 3799 3799 Size: 6229 PstI C_TGCA'G Cuts at: 830 3581 830 Size: 2751 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6229 PvuII CAG'CTG Cuts at: 2084 2654 3060 3287 2084 Size: 570 406 227 5026 Fragments arranged by size: 5026 570 406 227 SalI G'TCGA_C Cuts at: 1936 2429 3571 1936 Size: 493 1142 4594 Fragments arranged by size: 4594 1142 493 SapI GCTCTTCn'nnn_ Cuts at: 2369 3161 2369 Size: 792 5437 SbfI CC_TGCA'GG Cuts at: 3581 3581 Size: 6229 ScaI AGT'ACT Cuts at: 3321 4477 6189 3321 Size: 1156 1712 3361 Fragments arranged by size: 3361 1712 1156 SexAI A'CCwGG_T Cuts at: 3219 3219 Size: 6229 SfcI C'TryA_G Cuts at: 826 1045 2010 3009 3577 4712 5393 5584 826 Size: 219 965 999 568 1135 681 191 1471 Fragments arranged by size: 1471 1135 999 965 681 568 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6229 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6229 SmaI CCC'GGG Cuts at: 1872 2705 3556 1872 Size: 833 851 4545 Fragments arranged by size: 4545 851 833 SmlI C'TyrA_G Cuts at: 820 1017 1943 2449 2906 4333 5234 5496 820 Size: 197 926 506 457 1427 901 262 1553 Fragments arranged by size: 1553 1427 926 901 506 457 262 197 SnaBI TAC'GTA Cuts at: 493 4020 493 Size: 3527 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6229 SphI G_CATG'C Cuts at: 1132 3587 1132 Size: 2455 3774 SspI AAT'ATT Cuts at: 5 52 4153 5 Size: 47 4101 2081 Fragments arranged by size: 4101 2081 47 StuI AGG'CCT Cuts at: 1517 3007 1517 Size: 1490 4739 StyI C'CwwG_G Cuts at: 513 1065 2004 3169 3668 513 Size: 552 939 1165 499 3074 Fragments arranged by size: 3074 1165 939 552 499 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2048 2523 4256 4273 4426 4611 5056 Size: 1255 468 475 1733 17 153 185 445 Cuts at: 5056 325 Size: 1498 Fragments arranged by size: 1733 1498 1255 475 468 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3319 4475 6187 Size: 274 80 33 51 1443 1342 1156 1712 Cuts at: 6187 96 Size: 138 Fragments arranged by size: 1712 1443 1342 1156 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2016 2048 2429 2461 2943 2975 3021 3053 6189 Size: 32 381 32 482 32 46 32 3136 Cuts at: 6189 6221 2016 Size: 32 2024 Fragments arranged by size: 3136 2024 482 381 46 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3221 1316 Size: 1905 4324 XbaI T'CTAG_A Cuts at: 3565 3565 Size: 6229 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3211 3376 3491 3211 Size: 165 115 5949 Fragments arranged by size: 5949 165 115 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6229 XmaI C'CCGG_G Cuts at: 1870 2703 3554 1870 Size: 833 851 4545 Fragments arranged by size: 4545 851 833 XmnI GAAnn'nnTTC Cuts at: 3254 4358 3254 Size: 1104 5125 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4037 276 Size: 53 83 186 936 2503 2468 Fragments arranged by size: 2503 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI