(Linear) (Six-base) MAPSORT of: KIEE5660.seq Check: 1399 from: 1 to: 1524 KIEE5660.orf With 182 enzymes: SgfI * December 5, 2014 13:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 157 1524 Size: 157 1367 AccI GT'mk_AC Cuts at: 0 425 1524 Size: 425 1099 Acc65I G'GTAC_C Cuts at: 0 688 728 1524 Size: 688 40 796 Fragments arranged by size: 796 688 40 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 1137 1524 Size: 144 993 387 Fragments arranged by size: 993 387 144 AlwNI CAG_nnn'CTG Cuts at: 0 208 1524 Size: 208 1316 ApoI r'AATT_y Cuts at: 0 188 1524 Size: 188 1336 AvaI C'yCGr_G Cuts at: 0 698 1524 Size: 698 826 BanI G'GyrC_C Cuts at: 0 500 688 728 1064 1308 1524 Size: 500 188 40 336 244 216 Fragments arranged by size: 500 336 244 216 188 40 BanII G_rGCy'C Cuts at: 0 764 1044 1524 Size: 764 280 480 Fragments arranged by size: 764 480 280 BbsI GAAGACnn'nnnn_ Cuts at: 0 1242 1446 1524 Size: 1242 204 78 Fragments arranged by size: 1242 204 78 BglI GCCn_nnn'nGGC Cuts at: 0 278 1524 Size: 278 1246 BglII A'GATC_T Cuts at: 0 1191 1524 Size: 1191 333 Bme1580I G_kGCm'C Cuts at: 0 339 791 1311 1524 Size: 339 452 520 213 Fragments arranged by size: 520 452 339 213 BmrI ACTGGGnnnn_n' Cuts at: 0 1390 1408 1524 Size: 1390 18 116 Fragments arranged by size: 1390 116 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 214 230 934 1524 Size: 214 16 704 590 Fragments arranged by size: 704 590 214 16 Bpu10I CC'TnA_GC Cuts at: 0 1043 1524 Size: 1043 481 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 465 922 1524 Size: 465 457 602 Fragments arranged by size: 602 465 457 BsaI GGTCTCn'nnnn_ Cuts at: 0 224 1524 Size: 224 1300 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 672 702 938 968 1524 Size: 672 30 236 30 556 Fragments arranged by size: 672 556 236 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 604 673 946 1524 Size: 604 69 273 578 Fragments arranged by size: 604 578 273 69 BseYI C'CCAG_C Cuts at: 0 15 1445 1524 Size: 15 1430 79 Fragments arranged by size: 1430 79 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 798 1498 1524 Size: 798 700 26 Fragments arranged by size: 798 700 26 BsiHKAI G_wGCw'C Cuts at: 0 412 1409 1524 Size: 412 997 115 Fragments arranged by size: 997 412 115 BsmI GAATG_Cn' Cuts at: 0 1439 1524 Size: 1439 85 Bsp1286I G_dGCh'C Cuts at: 0 339 412 764 791 1044 1311 1409 1524 Size: 339 73 352 27 253 267 98 115 Fragments arranged by size: 352 339 267 253 115 98 73 27 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 157 1440 1524 Size: 157 1283 84 Fragments arranged by size: 1283 157 84 BsrBI CCG'CTC Cuts at: 0 906 1524 Size: 906 618 BstXI CCAn_nnnn'nTGG Cuts at: 0 226 942 1524 Size: 226 716 582 Fragments arranged by size: 716 582 226 BstYI r'GATC_y Cuts at: 0 593 1191 1524 Size: 593 598 333 Fragments arranged by size: 598 593 333 BtgI C'CryG_G Cuts at: 0 875 1524 Size: 875 649 BtsI GCAGTG_nn' Cuts at: 0 339 1510 1524 Size: 339 1171 14 Fragments arranged by size: 1171 339 14 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 510 545 1524 Size: 510 35 979 Fragments arranged by size: 979 510 35 DraI TTT'AAA Cuts at: 0 536 1524 Size: 536 988 DraIII CAC_nnn'GTG Cuts at: 0 798 1524 Size: 798 726 EaeI y'GGCC_r Cuts at: 0 543 1524 Size: 543 981 EarI CTCTTCn'nnn_ Cuts at: 0 63 330 364 1156 1524 Size: 63 267 34 792 368 Fragments arranged by size: 792 368 267 63 34 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 144 214 230 934 1137 1524 Size: 144 70 16 704 203 387 Fragments arranged by size: 704 387 203 144 70 16 EcoNI CCTnn'n_nnAGG Cuts at: 0 675 1524 Size: 675 849 EcoO109I rG'GnC_Cy Cuts at: 0 205 253 964 1524 Size: 205 48 711 560 Fragments arranged by size: 711 560 205 48 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 90 122 1524 Size: 90 32 1402 Fragments arranged by size: 1402 90 32 HincII GTy'rAC Cuts at: 0 426 1524 Size: 426 1098 KpnI G_GTAC'C Cuts at: 0 692 732 1524 Size: 692 40 792 Fragments arranged by size: 792 692 40 MfeI C'AATT_G Cuts at: 0 36 1524 Size: 36 1488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1103 1524 Size: 1103 421 MscI TGG'CCA Cuts at: 0 545 1524 Size: 545 979 MspA1I CmG'CkG Cuts at: 0 79 649 1055 1282 1524 Size: 79 570 406 227 242 Fragments arranged by size: 570 406 242 227 79 PflMI CCAn_nnn'nTGG Cuts at: 0 208 1464 1524 Size: 208 1256 60 Fragments arranged by size: 1256 208 60 PfoI T'CCnGG_A Cuts at: 0 1457 1524 Size: 1457 67 PpuMI rG'GwC_Cy Cuts at: 0 205 964 1524 Size: 205 759 560 Fragments arranged by size: 759 560 205 PvuII CAG'CTG Cuts at: 0 79 649 1055 1282 1524 Size: 79 570 406 227 242 Fragments arranged by size: 570 406 242 227 79 SalI G'TCGA_C Cuts at: 0 424 1524 Size: 424 1100 SapI GCTCTTCn'nnn_ Cuts at: 0 364 1156 1524 Size: 364 792 368 Fragments arranged by size: 792 368 364 ScaI AGT'ACT Cuts at: 0 1316 1524 Size: 1316 208 SexAI A'CCwGG_T Cuts at: 0 1214 1524 Size: 1214 310 SfcI C'TryA_G Cuts at: 0 5 1004 1524 Size: 5 999 520 Fragments arranged by size: 999 520 5 SmaI CCC'GGG Cuts at: 0 700 1524 Size: 700 824 SmlI C'TyrA_G Cuts at: 0 444 901 1524 Size: 444 457 623 Fragments arranged by size: 623 457 444 StuI AGG'CCT Cuts at: 0 1002 1524 Size: 1002 522 StyI C'CwwG_G Cuts at: 0 1164 1524 Size: 1164 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 43 518 1524 Size: 43 475 1006 Fragments arranged by size: 1006 475 43 TatI w'GTAC_w Cuts at: 0 1314 1524 Size: 1314 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 11 43 424 456 938 970 1016 1048 Size: 11 32 381 32 482 32 46 32 Cuts at: 1048 1524 Size: 476 Fragments arranged by size: 482 476 381 46 32 32 32 32 11 Tth111I GACn'n_nGTC Cuts at: 0 1216 1524 Size: 1216 308 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1206 1371 1486 1524 Size: 1206 165 115 38 Fragments arranged by size: 1206 165 115 38 XmaI C'CCGG_G Cuts at: 0 698 1524 Size: 698 826 XmnI GAAnn'nnTTC Cuts at: 0 1249 1524 Size: 1249 275 Enzymes that do cut: AarI AccI Acc65I AcuI AlwNI ApoI AvaI BanI BanII BbsI BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BstXI BstYI BtgI BtsI CspCI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I Hin4I HincII KpnI MfeI MmeI MscI MspA1I PflMI PfoI PpuMI PvuII SalI SapI ScaI SexAI SfcI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI Enzymes that do not cut: AatII AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI KasI MluI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SanDI SbfI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI ZraI