################################## # NO CUT ENZYMES ( 189 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspHI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbvCI BbvI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsoBI Bsp1286I Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRII EcoRV EcoT14I FauI FbaI Fnu4HI FokI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KspI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvaI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### Acc65I 224 GGTACC G^GTACC AcsI 346 GAATTC [GA]^AATT[CT] AocI 228 CCTGAGG CC^T[ACGT]AGG ApoI 346 GAATTC [GA]^AATT[CT] Asp700I 421 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 224 GGTACC G^GTACC AvaII 260 GGACC G^G[AT]CC BamHI 23 GGATCC G^GATCC BanI 224 GGTACC G^G[CT][GA]CC BbsI 200 GAAGAC GAAGAC(2/6) BciVI 11 GGATAC GTATCC(6/5) BfaI 70 CTAG C^TAG BpmI 107 CTCCAG CTGGAG(16/14) BpuAI 200 GAAGAC GAAGAC(2/6) BsiYI 517 CCTGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 517 CCTGTAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 259 GGGAC GGGAC(10/14) BsmI 394 GAATGC GAATGC(1/-1) BspDI 235 ATCGAT AT^CGAT BstYI 23 GGATCC [GA]^GATC[CT] Bsu36I 228 CCTGAGG CC^T[ACGT]AGG Cac8I 91 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 260 GGACC G^G[ACGT]CC ClaI 235 ATCGAT AT^CGAT DraII 259 GGGACCC [GA]G^G[ACGT]CC[CT] DrdI 203 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 205 CTCTTC CTCTTC(1/4) Eco81I 228 CCTGAGG CC^T[ACGT]AGG EcoNI 517 CCTGTAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 259 GGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 346 GAATTC G^AATTC EcoT22I 452 ATGCAT ATGCA^T KpnI 224 GGTACC GGTAC^C Ksp632I 205 CTCTTC CTCTTC(1/4) MaeI 70 CTAG C^TAG MaeIII 410 GTAAC ^GT[ACGT]AC MflI 23 GGATCC [GA]^GATC[CT] MseI 499 TTAA T^TAA MwoI 30 GCCAACTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NdeI 449 CATATG CA^TATG NsiI 452 ATGCAT ATGCA^T PpuMI 259 GGGACCC [GA]G^G[AT]CC[CT] Sau96I 260 GGACC G^G[ACGT]CC SfaNI 454 GCATC GCATC(5/9) SfcI 42 CTACAG C^T[GA][CT]AG TfiI 513 GATTC G^A[AT]TC Tru9I 499 TTAA T^TAA XhoII 23 GGATCC [GA]^GATC[CT] XmnI 421 GAACTAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 8 enzymes) # ################################### AfaI 77 GTAC GT^AC 225 GTAC AluI 90 AGCT AG^CT 459 AGCT DdeI 146 CTGAG C^T[ACGT]AG 229 CTGAG PleI 105 GACTC GAGTC(4/5) 203 GACTC RsaI 77 GTAC GT^AC 225 GTAC TaqI 213 TCGA T^CGA 236 TCGA TspRI 220 CAGTG CA[GC]TG[ACGT][ACGT]^ 305 CAGTG TthHB8I 213 TCGA T^CGA 236 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AlwI BsrDI DpnII HinfI MboI MboII MnlI NdeII NlaIII NlaIV Sau3AI Tsp509I