Restriction map for
pFN21AE9512
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BciVI BfrI BlnI
BsaBI BsaHI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HindIII KasI
KpnI KspI MamI MluI MroI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 970 GTCTAC GT^[AC][GT]AC
AcsI 1006 GAATTC [GA]^AATT[CT]
ApaI 485 GGGCCC GGGCC^C
ApoI 1006 GAATTC [GA]^AATT[CT]
AspHI 828 GAGCAC G[AT]GC[AT]^C
AspI 952 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 292 CTCGGG C^[CT]CG[GA]G
BanI 40 GGCACC G^G[CT][GA]CC
BbsI 1509 GAAGAC GAAGAC(2/6)
BcnI 651 CCGGG CC^[GC]GG
BfaI 1300 CTAG C^TAG
BglI 874 GCCAATTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 225 AGATCT A^GATCT
BpuAI 1509 GAAGAC GAAGAC(2/6)
BsaAI 331 TACGTG [CT]AC^GT[GA]
BsaI 1174 GAGACC GGTCTC(1/5)
BsgI 221 GTGCAG GTGCAG(16/14)
BsiHKAI 828 GAGCAC G[AT]GC[AT]^C
BsoBI 292 CTCGGG C^[CT]CG[GA]G
BspMI 203 ACCTGC ACCTGC(4/8)
EcoNI 818 CCTCCCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1006 GAATTC G^AATTC
HaeII 871 AGCGCC [GA]GCGC^[CT]
HapII 651 CCGG C^CGG
HgaI 281 GACGC GACGC(5/10)
HpaI 190 GTTAAC GTT^AAC
HpaII 651 CCGG C^CGG
MaeI 1300 CTAG C^TAG
MaeII 332 ACGT A^CGT
MfeI 876 CAATTG C^AATTG
MspI 651 CCGG C^CGG
MunI 876 CAATTG C^AATTG
NciI 651 CCGGG CC^[GC]GG
PflFI 952 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 57 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 674 AGGACCT [GA]G^G[AT]CC[CT]
ScaI 509 AGTACT AGT^ACT
SfcI 906 CTGTAG C^T[GA][CT]AG
SmlI 181 CTCAAG C^T[CT][GA]AG
Tth111I 952 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 57 CCAGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwI 552 GATCC GGATC(4/5)
752 GATCC
BalI 335 TGGCCA TGG^CCA
1226 TGGCCA
BclI 125 TGATCA T^GATCA
368 TGATCA
BpmI 688 CTCCAG CTGGAG(16/14)
1091 CTCCAG
BsmFI 279 GGGAC GGGAC(10/14)
1414 GTCCC
CfoI 762 GCGC GCG^C
872 GCGC
DraII 485 GGGCCCC [GA]G^G[ACGT]CC[CT]
674 AGGACCT
DrdI 1109 GACCCAAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1405 GACAAGTGTGTC
EaeI 335 TGGCCA [CT]^GGCC[GA]
1226 TGGCCA
EcoO109I 485 GGGCCCC [GA]G^G[ACGT]CC[CT]
674 AGGACCT
EcoT14I 671 CCAAGG C^C[AT][AT]GG
1229 CCAAGG
FbaI 125 TGATCA T^GATCA
368 TGATCA
HhaI 762 GCGC GCG^C
872 GCGC
HinP1I 762 GCGC G^CGC
872 GCGC
MluNI 335 TGGCCA TGG^CCA
1226 TGGCCA
MscI 335 TGGCCA TGG^CCA
1226 TGGCCA
MseI 191 TTAA T^TAA
1517 TTAA
MslI 129 CACCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
270 CATCCTCATG
PvuII 101 CAGCTG CAG^CTG
1097 CAGCTG
SfaNI 1521 GATGC GCATC(5/9)
443 GCATC
StuI 803 AGGCCT AGG^CCT
1248 AGGCCT
StyI 671 CCAAGG C^C[AT][AT]GG
1229 CCAAGG
TaqI 642 TCGA T^CGA
771 TCGA
Tru9I 191 TTAA T^TAA
1517 TTAA
Tsp509I 877 AATT ^AATT
1007 AATT
TthHB8I 642 TCGA T^CGA
771 TCGA
XcmI 868 CCAAGCGCCAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1417 CCAGTCACCCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AfaI AluI AlwNI AvaII
BanII BbvI BlpI BmyI Bpu1102I
BsaJI BsiYI BslI BsmAI Bsp1286I
BspHI BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
Cfr13I DdeI DpnII DraIII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HincII HindII HinfI HphI ItaI
Ksp632I MaeIII MboI MboII MflI
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PleI RcaI RsaI
Sau3AI Sau96I ScrFI TfiI TseI
Tsp45I TspRI XhoII