Restriction map for
pFN21AE6594
(Circular) (Six-base) MAPSORT of: pFN21AE6594.seq Check: 7499 from: 1 to: 4921
pFN21AE6594 4921 bp
With 182 enzymes: SgfI *
March 18, 2015 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2731 278
Size: 53 83 186 936 1195 2468
Fragments arranged by size:
2468 1195 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2264 1937
Size: 327 4594
Acc65I G'GTAC_C
Cuts at: 2242 2242
Size: 4921
AclI AA'CG_TT
Cuts at: 1563 3048 3421 1563
Size: 1485 373 3063
Fragments arranged by size:
3063 1485 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2222 2984 4368 4636 1632
Size: 590 762 1384 268 1917
Fragments arranged by size:
1917 1384 762 590 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 4724
AflIII A'CryG_T
Cuts at: 1203 3820 4797 1203
Size: 2617 977 1327
Fragments arranged by size:
2617 1327 977
AgeI A'CCGG_T
Cuts at: 3752 3752
Size: 4921
AhdI GACnn_n'nnGTC
Cuts at: 3650 3650
Size: 4921
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 4921
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 4921
AlwNI CAG_nnn'CTG
Cuts at: 2664 4236 2664
Size: 1572 3349
ApaI G_GGCC'C
Cuts at: 2103 2103
Size: 4921
ApaLI G'TGCA_C
Cuts at: 2978 4134 2978
Size: 1156 3765
ApoI r'AATT_y
Cuts at: 1493 2230 2456 2608 4717 1493
Size: 737 226 152 2109 1697
Fragments arranged by size:
2109 1697 737 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3475 160
Size: 872 2443 1606
Fragments arranged by size:
2443 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4921
AvaI C'yCGr_G
Cuts at: 1870 1943 2095 2246 1870
Size: 73 152 151 4545
Fragments arranged by size:
4545 152 151 73
BamHI G'GATC_C
Cuts at: 2251 2618 2251
Size: 367 4554
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2242 3697 618
Size: 325 217 627 455 1455 1842
Fragments arranged by size:
1842 1455 627 455 325 217
BanII G_rGCy'C
Cuts at: 1391 2103 2240 1391
Size: 712 137 4072
Fragments arranged by size:
4072 712 137
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 3950
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3112 3146 1903
Size: 34 50 34 1091 34 3678
Fragments arranged by size:
3678 1091 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2813 4023 2813
Size: 1210 3711
BclI T'GATC_A
Cuts at: 1548 1548
Size: 4921
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3532 136
Size: 107 122 71 631 605 1860 1525
Fragments arranged by size:
1860 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4916 4916
Size: 4921
BlpI GC'TnA_GC
Cuts at: 2338 2338
Size: 4921
Bme1580I G_kGCm'C
Cuts at: 1790 2103 2982 4138 1790
Size: 313 879 1156 2573
Fragments arranged by size:
2573 1156 879 313
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 4921
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2652 3610 442
Size: 416 53 83 399 24 1235 958 1753
Fragments arranged by size:
1753 1235 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 4921
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3581 1239
Size: 2342 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3046 3911 4209 3046
Size: 865 298 3758
Fragments arranged by size:
3758 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3584 882
Size: 617 2085 2219
Fragments arranged by size:
2219 2085 617
BsaAI yAC'GTr
Cuts at: 493 1204 2712 493
Size: 711 1508 2702
Fragments arranged by size:
2702 1508 711
BsaBI GATnn'nnATC
Cuts at: 4915 4915
Size: 4921
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2728 3110 3745 275
Size: 53 83 186 936 1195 382 635 1451
Fragments arranged by size:
1451 1195 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3354 3752 4026 4173 4584 3354
Size: 398 274 147 411 3691
Fragments arranged by size:
3691 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4696
Fragments arranged by size:
4696 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 4921
BseYI C'CCAG_C
Cuts at: 4124 4620 4124
Size: 496 4425
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4348
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2371 3132 4160 1540
Size: 275 186 370 761 1028 2301
Fragments arranged by size:
2301 1028 761 370 275 186
BsiHKAI G_wGCw'C
Cuts at: 2982 3067 4138 2982
Size: 85 1071 3765
Fragments arranged by size:
3765 1071 85
BsmI GAATG_Cn'
Cuts at: 2432 2525 2432
Size: 93 4828
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2103 2240 2982 3067 4138 1391
Size: 399 313 137 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 313 137 85
BspEI T'CCGG_A
Cuts at: 4584 4584
Size: 4921
BspHI T'CATG_A
Cuts at: 2810 4540 2810
Size: 1730 3191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2276 844
Size: 1432 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2078 2808 1394
Size: 429 255 730 3507
Fragments arranged by size:
3507 730 429 255
BsrDI GCAATG_nn'
Cuts at: 65 1248 3416 3590 65
Size: 1183 2168 174 1396
Fragments arranged by size:
2168 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3565 3752 1700
Size: 110 1755 187 2869
Fragments arranged by size:
2869 1755 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 4921
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 4921
BssSI C'ACGA_G
Cuts at: 2981 3993 2981
Size: 1012 3909
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 4921
BstBI TT'CG_AA
Cuts at: 3725 3725
Size: 4921
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 4921
BstYI r'GATC_y
Cuts at: 1972 2251 2618 3003 3020 4472 4558 4570 4916
Size: 279 367 385 17 1452 86 12 346
Cuts at: 4916 1972
Size: 1977
Fragments arranged by size:
1977 1452 385 367 346 279 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2127 513
Size: 552 1062 3307
Fragments arranged by size:
3307 1062 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4182
Fragments arranged by size:
4182 683 56
BtsI GCAGTG_nn'
Cuts at: 2437 3231 3251 2437
Size: 794 20 4107
Fragments arranged by size:
4107 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 4921
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 4886
DraI TTT'AAA
Cuts at: 2225 2225
Size: 4921
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3928 809
Size: 507 2612 1802
Fragments arranged by size:
2612 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2368 3257 4886
Size: 54 1425 50 275 18 538 889 1629
Cuts at: 4886 8
Size: 43
Fragments arranged by size:
1629 1425 889 538 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2368 1537
Size: 275 556 4090
Fragments arranged by size:
4090 556 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2851 1353
Size: 1498 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3508 3882 4028 387
Size: 1064 138 1919 374 146 1280
Fragments arranged by size:
1919 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2222 2984 3581 4368 4636 1239
Size: 393 590 762 597 787 268 1524
Fragments arranged by size:
1524 787 762 597 590 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2099 2100 2099
Size: 1 4920
EcoRI G'AATT_C
Cuts at: 2230 2608 2230
Size: 378 4543
FspI TGC'GCA
Cuts at: 3427 3427
Size: 4921
HaeII r_GCGC'y
Cuts at: 1678 2026 4068 1678
Size: 348 2042 2531
Fragments arranged by size:
2531 2042 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3562 3594 3636 3668 1373
Size: 32 135 32 1990 32 42 32 2626
Fragments arranged by size:
2626 1990 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2265 2511 669
Size: 988 281 327 246 3079
Fragments arranged by size:
3079 988 327 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 4921
HpaI GTT'AAC
Cuts at: 2511 2511
Size: 4921
KpnI G_GTAC'C
Cuts at: 2246 2246
Size: 4921
MfeI C'AATT_G
Cuts at: 2520 2520
Size: 4921
MluI A'CGCG_T
Cuts at: 4797 4797
Size: 4921
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 4035 4219 1305
Size: 817 1913 184 2007
Fragments arranged by size:
2007 1913 817 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3099
Fragments arranged by size:
3099 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2879 3238 3397 518
Size: 648 1713 359 159 2042
Fragments arranged by size:
2042 1713 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2113 2129 2180 2337 3014 4162 4407 1606
Size: 507 16 51 157 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 507 245 157 51 16
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4369
NdeI CA'TA_TG
Cuts at: 387 2185 387
Size: 1798 3123
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 4921
NotI GC'GGCC_GC
Cuts at: 2368 2368
Size: 4921
NspI r_CATG'y
Cuts at: 1132 1229 2279 3824 1132
Size: 97 1050 1545 2229
Fragments arranged by size:
2229 1545 1050 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 4921
PciI A'CATG_T
Cuts at: 3820 3820
Size: 4921
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 4921
PmeI GTTT'AAAC
Cuts at: 2225 2225
Size: 4921
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2990 3022 4503 4535 2990
Size: 32 1481 32 3376
Fragments arranged by size:
3376 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 4921
PsiI TTA'TAA
Cuts at: 2491 2491
Size: 4921
PspOMI G'GGCC_C
Cuts at: 2099 2099
Size: 4921
PstI C_TGCA'G
Cuts at: 830 2073 2273 830
Size: 1243 200 3478
Fragments arranged by size:
3478 1243 200
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 4921
PvuII CAG'CTG
Cuts at: 2180 2180
Size: 4921
SacII CC_GC'GG
Cuts at: 2130 2130
Size: 4921
SalI G'TCGA_C
Cuts at: 1936 2263 1936
Size: 327 4594
SbfI CC_TGCA'GG
Cuts at: 2273 2273
Size: 4921
ScaI AGT'ACT
Cuts at: 3169 4881 3169
Size: 1712 3209
SfcI C'TryA_G
Cuts at: 826 1045 2069 2269 3404 4085 4276 826
Size: 219 1024 200 1135 681 191 1471
Fragments arranged by size:
1471 1135 1024 681 219 200 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 4921
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 4921
SmaI CCC'GGG
Cuts at: 1872 2248 1872
Size: 376 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3025 3926 4188 820
Size: 197 926 1082 901 262 1553
Fragments arranged by size:
1553 1082 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 2712 493
Size: 2219 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 4921
SphI G_CATG'C
Cuts at: 1132 2279 1132
Size: 1147 3774
SspI AAT'ATT
Cuts at: 5 52 2845 5
Size: 47 2793 2081
Fragments arranged by size:
2793 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 4921
StyI C'CwwG_G
Cuts at: 513 1065 2360 513
Size: 552 1295 3074
Fragments arranged by size:
3074 1295 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2948 2965 3118 3303 3748 325
Size: 1255 1368 17 153 185 445 1498
Fragments arranged by size:
1498 1368 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3167 4879 96
Size: 274 80 33 51 1443 1190 1712 138
Fragments arranged by size:
1712 1443 1190 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2136 2168 4881 4913 2136
Size: 32 2713 32 2144
Fragments arranged by size:
2713 2144 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 4921
XbaI T'CTAG_A
Cuts at: 2257 2257
Size: 4921
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 4921
XmaI C'CCGG_G
Cuts at: 1870 2246 1870
Size: 376 4545
XmnI GAAnn'nnTTC
Cuts at: 2207 3050 2207
Size: 843 4078
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2729 276
Size: 53 83 186 936 1195 2468
Fragments arranged by size:
2468 1195 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI