################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I FbaI FseI FspI HgaI HincII HindII HindIII HpaI KpnI KspI MfeI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I Tth111I XcmI XhoI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 794 GGCGCC G[GA]^CG[CT]C AocI 1006 CCTAAGG CC^T[ACGT]AGG Aor51HI 548 AGCGCT AGC^GCT Asp700I 718 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbeI 794 GGCGCC GGCGC^C BcgI 1114 GCATCTGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 661 CTAG C^TAG BsaBI 735 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 794 GGCGCC G[GA]^CG[CT]C BsmI 671 GCATTC GAATGC(1/-1) Bsp1407I 1091 TGTACA T^GTACA BspHI 839 TCATGA T^CATGA BsrDI 537 CATTGC GCAATG(2/0) BsrFI 857 ACCGGC [GA]^CCGG[CT] BsrGI 1091 TGTACA T^GTACA BsrI 357 ACTGG ACTGG(1/-1) BssSI 727 CACGAG CACGAG(-5/-1) Bsu36I 1006 CCTAAGG CC^T[ACGT]AGG Cfr10I 857 ACCGGC [GA]^CCGG[CT] DraIII 727 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eco47III 548 AGCGCT AGC^GCT Eco81I 1006 CCTAAGG CC^T[ACGT]AGG EcoRI 448 GAATTC G^AATTC EcoRV 709 GATATC GAT^ATC EcoT22I 247 ATGCAT ATGCA^T Hin1I 794 GGCGCC G[GA]^CG[CT]C KasI 794 GGCGCC G^GCGCC MaeI 661 CTAG C^TAG MaeII 191 ACGT A^CGT MamI 735 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NarI 794 GGCGCC GG^CGCC NcoI 774 CCATGG C^CATGG NsiI 247 ATGCAT ATGCA^T PshAI 186 GACCCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 839 TCATGA T^CATGA SacI 505 GAGCTC GAGCT^C SapI 507 GCTCTTC GCTCTTC(1/4) SfoI 794 GGCGCC GGC^GCC SspBI 1091 TGTACA T^GTACA XbaI 660 TCTAGA T^CTAGA XmnI 718 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 1092 GTAC GT^AC 1167 GTAC AvaI 227 CCCGGG C^[CT]CG[GA]G 870 CCCGGG BanI 465 GGTGCC G^G[CT][GA]CC 794 GGCGCC BglII 137 AGATCT A^GATCT 786 AGATCT BpmI 238 CTGGAG CTGGAG(16/14) 1147 CTGGAG BseRI 241 GAGGAG GAGGAG(10/8) 934 GAGGAG BsgI 270 CTGCAC GTGCAG(16/14) 1058 GTGCAG BsoBI 227 CCCGGG C^[CT]CG[GA]G 870 CCCGGG BstF5I 1161 CATCC GGATG(2/0) 768 GGATG BstXI 774 CCATGGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1205 CCAACATGGTGG FauI 462 GCGGG CCCGC(4/6) 897 GCGGG FokI 1161 CATCC GGATG(9/13) 768 GGATG HaeII 548 AGCGCT [GA]GCGC^[CT] 794 GGCGCC MaeIII 610 GTCAC ^GT[ACGT]AC 686 GTCAC MslI 775 CATGGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1209 CATGGTGGTG PflMI 333 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1140 CCAGGACCTGG PleI 289 GAGTC GAGTC(4/5) 646 GAGTC RsaI 1092 GTAC GT^AC 1167 GTAC SmaI 227 CCCGGG CCC^GGG 870 CCCGGG SmlI 141 CTTGAG C^T[CT][GA]AG 799 CTCAAG TfiI 567 GAATC G^A[AT]TC 846 GATTC Tsp45I 610 GTCAC ^GT[GC]AC 686 GTCAC Van91I 333 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1140 CCAGGACCTGG XmaCI 227 CCCGGG C^CCGGG 870 CCCGGG XmaI 227 CCCGGG C^CCGGG 870 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcsI AluI AlwI AlwNI ApoI AspHI AvaII BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TseI Tsp509I TspRI TthHB8I XhoII