################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BseRI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI Eam1105I Eco47III Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KasI KspI MaeI MamI MfeI MluI MluNI MroI MscI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfaNI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TfiI Tru9I Tth111I TthHB8I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Acc65I 437 GGTACC G^GTACC AcsI 21 GAATTT [GA]^AATT[CT] AcyI 649 GGCGTC G[GA]^CG[CT]C ApoI 21 GAATTT [GA]^AATT[CT] Asp700I 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 437 GGTACC G^GTACC BamHI 447 GGATCC G^GATCC BanI 437 GGTACC G^G[CT][GA]CC BpmI 574 CTCCAG CTGGAG(16/14) BsaHI 649 GGCGTC G[GA]^CG[CT]C BsaI 55 GAGACC GGTCTC(1/5) BsaWI 493 ACCGGA [AT]^CCGG[AT] BsiEI 665 CGGCCG CG[GA][CT]^CG BsmAI 55 GAGAC GTCTC(1/5) BssSI 167 CACGAG CACGAG(-5/-1) BstYI 447 GGATCC [GA]^GATC[CT] DdeI 296 CTGAG C^T[ACGT]AG DpnII 448 GATC ^GATC DsaI 113 CCGTGG C^C[GA][CT]GG EagI 665 CGGCCG C^GGCCG EarI 64 GAAGAG CTCTTC(1/4) EclXI 665 CGGCCG C^GGCCG Eco52I 665 CGGCCG C^GGCCG EcoNI 588 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 239 CCAAGG C^C[AT][AT]GG HgaI 650 GCGTC GACGC(5/10) Hin1I 649 GGCGTC G[GA]^CG[CT]C HphI 236 TCACC GGTGA(8/7) KpnI 437 GGTACC GGTAC^C Ksp632I 64 GAAGAG CTCTTC(1/4) MaeIII 591 GTCAC ^GT[ACGT]AC MboI 448 GATC ^GATC MflI 447 GGATCC [GA]^GATC[CT] NdeII 448 GATC ^GATC PflMI 33 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 7 CAGCTG CAG^CTG Sau3AI 448 GATC ^GATC SbfI 441 CCTGCAGG CCTGCA^GG Sse8387I 441 CCTGCAGG CCTGCA^GG StyI 239 CCAAGG C^C[AT][AT]GG Tsp45I 591 GTCAC ^GT[GC]AC Tsp509I 22 AATT ^AATT TspRI 397 CACTG CA[GC]TG[ACGT][ACGT]^ Van91I 33 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 447 GGATCC [GA]^GATC[CT] XmnI 348 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 336 GTAC GT^AC 438 GTAC AlwI 448 GATCC GGATC(4/5) 447 GGATC AlwNI 262 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG 583 CAGGTCCTG AspHI 205 GAGCTC G[AT]GC[AT]^C 478 GAGCTC BanII 205 GAGCTC G[GA]GC[CT]^C 478 GAGCTC BbsI 311 GAAGAC GAAGAC(2/6) 390 GAAGAC BpuAI 311 GAAGAC GAAGAC(2/6) 390 GAAGAC BsiHKAI 205 GAGCTC G[AT]GC[AT]^C 478 GAGCTC BsrBI 69 GAGCGG CCGCTC(-3/-3) 551 GAGCGG BsrI 521 ACTGG ACTGG(1/-1) 671 CCAGT FauI 357 CCCGC CCCGC(4/6) 606 GCGGG HaeII 83 AGCGCC [GA]GCGC^[CT] 264 GGCGCT MaeII 474 ACGT A^CGT 712 ACGT MspA1I 7 CAGCTG C[AC]G^C[GT]G 358 CCGCTG PpuMI 321 GGGACCT [GA]G^G[AT]CC[CT] 584 AGGTCCT RsaI 336 GTAC GT^AC 438 GTAC SacI 205 GAGCTC GAGCT^C 478 GAGCTC SfiI 117 GGCCCCTGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 126 GGCCTGCGAGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 46 enzymes) # ######################################### AccII AciI AluI AvaII BbvI BcnI BglI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BssKI BstNI BstUI Cac8I CfoI Cfr13I DraII EaeI EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboII MnlI MspI MvaI MvnI MwoI NciI NlaIV PleI PstI Sau96I ScrFI SfcI TseI