################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AlwI ApoI Asp700I Asp718I AspI AviII AvrII BamHI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaI BsaWI BseAI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstYI BstZ17I CelII ClaI DraI DraIII EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KpnI KspI MaeI MamI MfeI MflI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I SacII SalI SbfI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI TfiI Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 72 enzymes) # ################################### AcyI 306 GGCGCC G[GA]^CG[CT]C AhdI 19 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 324 GTGCAC G^TGCAC AlwNI 289 CAGACCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 951 CCTTAGG CC^T[ACGT]AGG Aor51HI 398 AGCGCT AGC^GCT ApaI 737 GGGCCC GGGCC^C ApaLI 324 GTGCAC G^TGCAC AscI 138 GGCGCGCC GG^CGCGCC AseI 916 ATTAAT AT^TAAT AsnI 916 ATTAAT AT^TAAT AspEI 19 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 362 TGGCCA TGG^CCA BbeI 306 GGCGCC GGCGC^C BbrPI 202 CACGTG CAC^GTG BglI 722 GCCGGGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 394 CTGGAG CTGGAG(16/14) BsaAI 202 CACGTG [CT]AC^GT[GA] BsaHI 306 GGCGCC G[GA]^CG[CT]C BseRI 772 GAGGAG GAGGAG(10/8) BsgI 165 GTGCAG GTGCAG(16/14) BsiWI 275 CGTACG C^GTACG BspHI 803 TCATGA T^CATGA BsrDI 5 GCAATG GCAATG(2/0) BsrFI 172 GCCGGC [GA]^CCGG[CT] BssHII 139 GCGCGC G^CGCGC BstEII 210 GGTGACC G^GT[ACGT]ACC BstPI 210 GGTGACC G^GT[ACGT]ACC BstXI 386 CCATGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 951 CCTTAGG CC^T[ACGT]AGG Cfr10I 172 GCCGGC [GA]^CCGG[CT] CpoI 577 CGGACCG CG^G[AT]CCG DpnII 411 GATC ^GATC DrdI 931 GACCCTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 19 GACTCCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 398 AGCGCT AGC^GCT Eco81I 951 CCTTAGG CC^T[ACGT]AGG EcoO65I 210 GGTGACC G^GT[ACGT]ACC Hin1I 306 GGCGCC G[GA]^CG[CT]C HinfI 19 GACTC G^A[ACGT]TC KasI 306 GGCGCC G^GCGCC MaeIII 211 GTGAC ^GT[ACGT]AC MboI 411 GATC ^GATC MluNI 362 TGGCCA TGG^CCA MscI 362 TGGCCA TGG^CCA MslI 666 CATCCGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 172 GCCGGC GCC^GGC NarI 306 GGCGCC GG^CGCC NdeII 411 GATC ^GATC NgoMIV 172 GCCGGC G^CCGGC NspI 671 GCATGC [GA]CATG^[CT] PleI 19 GACTC GAGTC(4/5) PmaCI 202 CACGTG CAC^GTG PmlI 202 CACGTG CAC^GTG PshBI 916 ATTAAT AT^TAAT PstI 589 CTGCAG CTGCA^G PvuI 410 CGATCG CGAT^CG PvuII 592 CAGCTG CAG^CTG RcaI 803 TCATGA T^CATGA RsrII 577 CGGACCG CG^G[AT]CCG SacI 183 GAGCTC GAGCT^C Sau3AI 411 GATC ^GATC ScaI 539 AGTACT AGT^ACT SexAI 553 ACCTGGT A^CC[AT]GGT SfcI 589 CTGCAG C^T[GA][CT]AG SfiI 721 GGCCGGGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 306 GGCGCC GGC^GCC SphI 671 GCATGC GCATG^C StuI 955 AGGCCT AGG^CCT TaqI 409 TCGA T^CGA Tsp45I 211 GTGAC ^GT[GC]AC TthHB8I 409 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### BanI 306 GGCGCC G^G[CT][GA]CC 416 GGCACC BanII 183 GAGCTC G[GA]GC[CT]^C 737 GGGCCC BcgI 368 CGATGCTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 326 GCACCTACTTCG DsaI 386 CCATGG C^C[GA][CT]GG 764 CCATGG EagI 98 CGGCCG C^GGCCG 174 CGGCCG EclXI 98 CGGCCG C^GGCCG 174 CGGCCG Eco52I 98 CGGCCG C^GGCCG 174 CGGCCG FauI 740 CCCGC CCCGC(4/6) 150 GCGGG HgaI 42 GACGC GACGC(5/10) 440 GCGTC MseI 917 TTAA T^TAA 1006 TTAA NcoI 386 CCATGG C^CATGG 764 CCATGG PflMI 201 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 288 CCAGACCCTGG SapI 378 GCTCTTC GCTCTTC(1/4) 495 GCTCTTC SmaI 547 CCCGGG CCC^GGG 733 CCCGGG Tru9I 917 TTAA T^TAA 1006 TTAA Van91I 201 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 288 CCAGACCCTGG XmaCI 547 CCCGGG C^CCGGG 733 CCCGGG XmaI 547 CCCGGG C^CCGGG 733 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaI AvaII BbvI BcnI BmyI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DraII EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PpuMI RsaI Sau96I ScrFI SfaNI StyI TseI Tsp509I TspRI