(Circular) (Six-base) MAPSORT of: pFN21AE2290.seq Check: 5556 from: 1 to: 5779 pFN21AE2290 5779 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3589 278 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 1937 Size: 1185 4594 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2344 2635 3842 5226 5494 1632 Size: 712 291 1207 1384 268 1917 Fragments arranged by size: 1917 1384 1207 712 291 268 AfeI AGC'GCT Cuts at: 2405 2405 Size: 5779 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 2029 4508 2029 Size: 2479 3300 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 2299 3522 5094 2299 Size: 1223 1572 2984 Fragments arranged by size: 2984 1572 1223 ApaI G_GGCC'C Cuts at: 2746 2746 Size: 5779 ApaLI G'TGCA_C Cuts at: 2329 3836 4992 2329 Size: 1507 1156 3116 Fragments arranged by size: 3116 1507 1156 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AscI GG'CGCG_CC Cuts at: 2144 2144 Size: 5779 AseI AT'TA_AT Cuts at: 160 1032 2922 4333 160 Size: 872 1890 1411 1606 Fragments arranged by size: 1890 1606 1411 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2243 2315 2552 2738 3104 1870 Size: 73 300 72 237 186 366 4545 Fragments arranged by size: 4545 366 300 237 186 73 72 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2311 2421 3100 4555 618 Size: 325 217 627 524 110 679 1455 1842 Fragments arranged by size: 1842 1455 679 627 524 325 217 110 BanII G_rGCy'C Cuts at: 1391 2192 2746 3098 1391 Size: 801 554 352 4072 Fragments arranged by size: 4072 801 554 352 BbeI G_GCGC'C Cuts at: 2315 2315 Size: 5779 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4808 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2320 2354 2362 2396 3970 4004 1903 Size: 34 383 34 8 34 1574 34 3678 Fragments arranged by size: 3678 1574 383 34 34 34 34 8 BciVI GTATCCnnnnn_n' Cuts at: 3671 4881 3671 Size: 1210 4569 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2733 4390 136 Size: 107 122 71 631 605 1061 1657 1525 Fragments arranged by size: 1657 1525 1061 631 605 122 107 71 BglII A'GATC_T Cuts at: 5774 5774 Size: 5779 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2333 2424 2746 3840 4996 1790 Size: 543 91 322 1094 1156 2573 Fragments arranged by size: 2573 1156 1094 543 322 91 BmgBI CAC'GTC Cuts at: 1318 2164 1318 Size: 846 4933 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2023 2258 3510 Size: 416 53 83 399 24 606 235 1252 Cuts at: 3510 4468 442 Size: 958 1753 Fragments arranged by size: 1753 1252 958 606 416 399 235 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 4439 1239 Size: 1181 2019 2579 Fragments arranged by size: 2579 2019 1181 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3904 4769 5067 3904 Size: 865 298 4616 Fragments arranged by size: 4616 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4442 882 Size: 617 2943 2219 Fragments arranged by size: 2943 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2209 3570 493 Size: 711 1005 1361 2702 Fragments arranged by size: 2702 1361 1005 711 BsaBI GATnn'nnATC Cuts at: 5773 5773 Size: 5779 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2312 3586 3968 4603 Size: 53 83 186 936 779 1274 382 635 Cuts at: 4603 275 Size: 1451 Fragments arranged by size: 1451 1274 936 779 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4212 4610 4884 5031 5442 4212 Size: 398 274 147 411 4549 Fragments arranged by size: 4549 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5554 Fragments arranged by size: 5554 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2792 1186 Size: 1606 4173 BseYI C'CCAG_C Cuts at: 2077 2113 4982 5478 2077 Size: 36 2869 496 2378 Fragments arranged by size: 2869 2378 496 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2191 1163 Size: 573 455 4751 Fragments arranged by size: 4751 573 455 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2106 2182 2418 3229 3990 5018 Size: 275 186 105 76 236 811 761 1028 Cuts at: 5018 1540 Size: 2301 Fragments arranged by size: 2301 1028 811 761 275 236 186 105 76 BsiHKAI G_wGCw'C Cuts at: 2192 2333 2640 3840 3925 4996 2192 Size: 141 307 1200 85 1071 2975 Fragments arranged by size: 2975 1200 1071 307 141 85 BsiWI C'GTAC_G Cuts at: 2280 2280 Size: 5779 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 BsmBI CGTCTCn'nnnn_ Cuts at: 2039 2452 2774 2039 Size: 413 322 5044 Fragments arranged by size: 5044 413 322 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2192 2333 2424 2640 2746 3098 3840 Size: 399 402 141 91 216 106 352 742 Cuts at: 3840 3925 4996 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 402 399 352 216 141 106 91 85 BspEI T'CCGG_A Cuts at: 5442 5442 Size: 5779 BspHI T'CATG_A Cuts at: 2808 3668 5398 2808 Size: 860 1730 3189 Fragments arranged by size: 3189 1730 860 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2247 2258 2601 3134 844 Size: 1403 11 343 533 3489 Fragments arranged by size: 3489 1403 533 343 11 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2017 4274 4448 65 Size: 1183 769 2257 174 1396 Fragments arranged by size: 2257 1396 1183 769 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2177 4423 4610 1700 Size: 110 367 2246 187 2869 Fragments arranged by size: 2869 2246 367 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5779 BssHII G'CGCG_C Cuts at: 1925 2144 1925 Size: 219 5560 BssSI C'ACGA_G Cuts at: 3839 4851 3839 Size: 1012 4767 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5779 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 2215 1184 Size: 1031 4748 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2398 Size: 5779 BstYI r'GATC_y Cuts at: 1972 3109 3476 3861 3878 5330 5416 5428 5774 Size: 1137 367 385 17 1452 86 12 346 Cuts at: 5774 1972 Size: 1977 Fragments arranged by size: 1977 1452 1137 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2957 2957 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2004 2391 2769 513 Size: 552 939 387 378 3523 Fragments arranged by size: 3523 939 552 387 378 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 2728 3295 4089 4109 2728 Size: 567 794 20 4398 Fragments arranged by size: 4398 794 567 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5779 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2942 4786 809 Size: 507 1626 1844 1802 Fragments arranged by size: 1844 1802 1626 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2103 2179 2367 Size: 54 1425 50 275 18 273 76 188 Cuts at: 2367 2466 2725 3226 4115 5744 8 Size: 99 259 501 889 1629 43 Fragments arranged by size: 1629 1425 889 501 275 273 259 188 99 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2103 2179 3226 1537 Size: 275 291 76 1047 4090 Fragments arranged by size: 4090 1047 291 275 76 EarI CTCTTCn'nnn_ Cuts at: 1353 2390 2507 2878 3709 1353 Size: 1037 117 371 831 3423 Fragments arranged by size: 3423 1037 831 371 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2596 2789 4366 4740 4886 387 Size: 1064 138 1007 193 1577 374 146 1280 Fragments arranged by size: 1577 1280 1064 1007 374 193 146 138 EcoICRI GAG'CTC Cuts at: 2190 2190 Size: 5779 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2344 2420 2635 3842 4439 5226 5494 Size: 393 712 76 215 1207 597 787 268 Cuts at: 5494 1239 Size: 1524 Fragments arranged by size: 1524 1207 787 712 597 393 268 215 76 EcoO109I rG'GnC_Cy Cuts at: 2247 2461 2556 2742 2893 2247 Size: 214 95 186 151 5133 Fragments arranged by size: 5133 214 186 151 95 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2138 2178 2315 2407 2443 4926 1678 Size: 460 40 137 92 36 2483 2531 Fragments arranged by size: 2531 2483 460 137 92 40 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2015 2047 4420 4452 4494 Size: 32 135 32 443 32 2373 32 42 Cuts at: 4494 4526 1373 Size: 32 2626 Fragments arranged by size: 2626 2373 443 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3123 3369 669 Size: 988 281 1185 246 3079 Fragments arranged by size: 3079 1185 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5779 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KasI G'GCGC_C Cuts at: 2311 2311 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2981 4893 5077 1305 Size: 1676 1912 184 2007 Fragments arranged by size: 2007 1912 1676 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2369 10 Size: 54 1425 343 537 3420 Fragments arranged by size: 3420 1425 537 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2675 3737 4096 4255 518 Size: 648 1509 1062 359 159 2042 Fragments arranged by size: 2042 1509 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2151 2518 2599 2703 3195 3872 5020 5265 Size: 545 367 81 104 492 677 1148 245 Cuts at: 5265 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 545 492 367 245 104 81 NaeI GCC'GGC Cuts at: 2179 2179 Size: 5779 NarI GG'CG_CC Cuts at: 2312 2312 Size: 5779 NcoI C'CATG_G Cuts at: 513 1065 2004 2391 2769 513 Size: 552 939 387 378 3523 Fragments arranged by size: 3523 939 552 387 378 NdeI CA'TA_TG Cuts at: 387 387 Size: 5779 NgoMIV G'CCGG_C Cuts at: 2177 2177 Size: 5779 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2680 3137 4682 1132 Size: 97 1451 457 1545 2229 Fragments arranged by size: 2229 1545 1451 457 97 PasI CC'CwG_GG Cuts at: 1348 2716 1348 Size: 1368 4411 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 2299 1108 Size: 1104 87 4588 Fragments arranged by size: 4588 1104 87 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PmlI CAC'GTG Cuts at: 2209 2209 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2213 2245 3848 3880 5361 5393 2213 Size: 32 1603 32 1481 32 2599 Fragments arranged by size: 2599 1603 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2247 2556 2893 2247 Size: 309 337 5133 Fragments arranged by size: 5133 337 309 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspOMI G'GGCC_C Cuts at: 2742 2742 Size: 5779 PstI C_TGCA'G Cuts at: 830 2598 3131 830 Size: 1768 533 3478 Fragments arranged by size: 3478 1768 533 PvuI CG_AT'CG Cuts at: 2001 2418 2001 Size: 417 5362 PvuII CAG'CTG Cuts at: 2599 2599 Size: 5779 RsrII CG'GwC_CG Cuts at: 2583 2583 Size: 5779 SacI G_AGCT'C Cuts at: 2192 2192 Size: 5779 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SapI GCTCTTCn'nnn_ Cuts at: 2390 2507 2390 Size: 117 5662 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 2546 4027 5739 2546 Size: 1481 1712 2586 Fragments arranged by size: 2586 1712 1481 SexAI A'CCwGG_T Cuts at: 2558 2558 Size: 5779 SfcI C'TryA_G Cuts at: 826 1045 2594 3127 4262 4943 5134 826 Size: 219 1549 533 1135 681 191 1471 Fragments arranged by size: 1549 1471 1135 681 533 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2733 2733 Size: 5779 SfoI GGC'GCC Cuts at: 2313 2313 Size: 5779 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 2554 2740 3106 1872 Size: 682 186 366 4545 Fragments arranged by size: 4545 682 366 186 SmlI C'TyrA_G Cuts at: 820 1017 1943 3883 4784 5046 820 Size: 197 926 1940 901 262 1553 Fragments arranged by size: 1940 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 2680 3137 1132 Size: 1548 457 3774 Fragments arranged by size: 3774 1548 457 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 2962 1517 Size: 1445 4334 StyI C'CwwG_G Cuts at: 513 1065 2004 2391 2769 2939 3218 513 Size: 552 939 387 378 170 279 3074 Fragments arranged by size: 3074 939 552 387 378 279 170 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2234 3806 3823 3976 4161 4606 325 Size: 1255 654 1572 17 153 185 445 1498 Fragments arranged by size: 1572 1498 1255 654 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2544 4025 5737 Size: 274 80 33 51 1443 567 1481 1712 Cuts at: 5737 96 Size: 138 Fragments arranged by size: 1712 1481 1443 567 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2153 2185 5739 5771 2153 Size: 32 3554 32 2161 Fragments arranged by size: 3554 2161 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5779 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5779 XmaI C'CCGG_G Cuts at: 1870 2552 2738 3104 1870 Size: 682 186 366 4545 Fragments arranged by size: 4545 682 366 186 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3587 276 Size: 53 83 186 936 2053 2468 Fragments arranged by size: 2468 2053 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspXI PsrI SacII SanDI SrfI SwaI XcmI