(Linear) (Six-base) MAPSORT of: KIEE2290.seq Check: 6496 from: 1 to: 1074 KIEE2290.orf With 182 enzymes: SgfI * July 15, 2010 18:57 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 339 630 1074 Size: 339 291 444 Fragments arranged by size: 444 339 291 AfeI AGC'GCT Cuts at: 0 400 1074 Size: 400 674 AhdI GACnn_n'nnGTC Cuts at: 0 24 1074 Size: 24 1050 AlwNI CAG_nnn'CTG Cuts at: 0 294 1074 Size: 294 780 ApaI G_GGCC'C Cuts at: 0 741 1074 Size: 741 333 ApaLI G'TGCA_C Cuts at: 0 324 1074 Size: 324 750 AscI GG'CGCG_CC Cuts at: 0 139 1074 Size: 139 935 AseI AT'TA_AT Cuts at: 0 917 1074 Size: 917 157 AvaI C'yCGr_G Cuts at: 0 238 310 547 733 1074 Size: 238 72 237 186 341 Fragments arranged by size: 341 238 237 186 72 BanI G'GyrC_C Cuts at: 0 306 416 1074 Size: 306 110 658 Fragments arranged by size: 658 306 110 BanII G_rGCy'C Cuts at: 0 187 741 1074 Size: 187 554 333 Fragments arranged by size: 554 333 187 BbeI G_GCGC'C Cuts at: 0 310 1074 Size: 310 764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 315 349 357 391 1074 Size: 315 34 8 34 683 Fragments arranged by size: 683 315 34 34 8 BglI GCCn_nnn'nGGC Cuts at: 0 728 1074 Size: 728 346 Bme1580I G_kGCm'C Cuts at: 0 328 419 741 1074 Size: 328 91 322 333 Fragments arranged by size: 333 328 322 91 BmgBI CAC'GTC Cuts at: 0 159 1074 Size: 159 915 BmrI ACTGGGnnnn_n' Cuts at: 0 18 253 1074 Size: 18 235 821 Fragments arranged by size: 821 235 18 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 415 1074 Size: 415 659 BsaAI yAC'GTr Cuts at: 0 204 1074 Size: 204 870 BsaHI Gr'CG_yC Cuts at: 0 307 1074 Size: 307 767 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 787 1074 Size: 787 287 BseYI C'CCAG_C Cuts at: 0 72 108 1074 Size: 72 36 966 Fragments arranged by size: 966 72 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 186 1074 Size: 186 888 BsiEI CG_ry'CG Cuts at: 0 101 177 413 1074 Size: 101 76 236 661 Fragments arranged by size: 661 236 101 76 BsiHKAI G_wGCw'C Cuts at: 0 187 328 635 1074 Size: 187 141 307 439 Fragments arranged by size: 439 307 187 141 BsiWI C'GTAC_G Cuts at: 0 275 1074 Size: 275 799 BsmBI CGTCTCn'nnnn_ Cuts at: 0 34 447 769 1074 Size: 34 413 322 305 Fragments arranged by size: 413 322 305 34 Bsp1286I G_dGCh'C Cuts at: 0 187 328 419 635 741 1074 Size: 187 141 91 216 106 333 Fragments arranged by size: 333 216 187 141 106 91 BspHI T'CATG_A Cuts at: 0 803 1074 Size: 803 271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 242 253 596 1074 Size: 242 11 343 478 Fragments arranged by size: 478 343 242 11 BsrDI GCAATG_nn' Cuts at: 0 12 1074 Size: 12 1062 BsrFI r'CCGG_y Cuts at: 0 172 1074 Size: 172 902 BssHII G'CGCG_C Cuts at: 0 139 1074 Size: 139 935 BstEII G'GTnAC_C Cuts at: 0 210 1074 Size: 210 864 BstXI CCAn_nnnn'nTGG Cuts at: 0 393 1074 Size: 393 681 Bsu36I CC'TnA_GG Cuts at: 0 952 1074 Size: 952 122 BtgI C'CryG_G Cuts at: 0 386 764 1074 Size: 386 378 310 Fragments arranged by size: 386 378 310 BtsI GCAGTG_nn' Cuts at: 0 723 1074 Size: 723 351 DrdI GACnn_nn'nnGTC Cuts at: 0 937 1074 Size: 937 137 EaeI y'GGCC_r Cuts at: 0 98 174 362 461 720 1074 Size: 98 76 188 99 259 354 Fragments arranged by size: 354 259 188 99 98 76 EagI C'GGCC_G Cuts at: 0 98 174 1074 Size: 98 76 900 Fragments arranged by size: 900 98 76 EarI CTCTTCn'nnn_ Cuts at: 0 385 502 873 1074 Size: 385 117 371 201 Fragments arranged by size: 385 371 201 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 591 784 1074 Size: 591 193 290 Fragments arranged by size: 591 290 193 EcoICRI GAG'CTC Cuts at: 0 185 1074 Size: 185 889 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 339 415 630 1074 Size: 339 76 215 444 Fragments arranged by size: 444 339 215 76 EcoO109I rG'GnC_Cy Cuts at: 0 242 456 551 737 888 1074 Size: 242 214 95 186 151 186 Fragments arranged by size: 242 214 186 186 151 95 HaeII r_GCGC'y Cuts at: 0 133 173 310 402 438 1074 Size: 133 40 137 92 36 636 Fragments arranged by size: 636 137 133 92 40 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 10 42 1074 Size: 10 32 1032 Fragments arranged by size: 1032 32 10 KasI G'GCGC_C Cuts at: 0 306 1074 Size: 306 768 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 976 1074 Size: 976 98 MscI TGG'CCA Cuts at: 0 364 1074 Size: 364 710 MslI CAynn'nnrTG Cuts at: 0 670 1074 Size: 670 404 MspA1I CmG'CkG Cuts at: 0 146 513 594 698 1074 Size: 146 367 81 104 376 Fragments arranged by size: 376 367 146 104 81 NaeI GCC'GGC Cuts at: 0 174 1074 Size: 174 900 NarI GG'CG_CC Cuts at: 0 307 1074 Size: 307 767 NcoI C'CATG_G Cuts at: 0 386 764 1074 Size: 386 378 310 Fragments arranged by size: 386 378 310 NgoMIV G'CCGG_C Cuts at: 0 172 1074 Size: 172 902 NspI r_CATG'y Cuts at: 0 675 1074 Size: 675 399 PasI CC'CwG_GG Cuts at: 0 711 1074 Size: 711 363 PflMI CCAn_nnn'nTGG Cuts at: 0 207 294 1074 Size: 207 87 780 Fragments arranged by size: 780 207 87 PmlI CAC'GTG Cuts at: 0 204 1074 Size: 204 870 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 208 240 1074 Size: 208 32 834 Fragments arranged by size: 834 208 32 PpuMI rG'GwC_Cy Cuts at: 0 242 551 888 1074 Size: 242 309 337 186 Fragments arranged by size: 337 309 242 186 PspOMI G'GGCC_C Cuts at: 0 737 1074 Size: 737 337 PstI C_TGCA'G Cuts at: 0 593 1074 Size: 593 481 PvuI CG_AT'CG Cuts at: 0 413 1074 Size: 413 661 PvuII CAG'CTG Cuts at: 0 594 1074 Size: 594 480 RsrII CG'GwC_CG Cuts at: 0 578 1074 Size: 578 496 SacI G_AGCT'C Cuts at: 0 187 1074 Size: 187 887 SapI GCTCTTCn'nnn_ Cuts at: 0 385 502 1074 Size: 385 117 572 Fragments arranged by size: 572 385 117 ScaI AGT'ACT Cuts at: 0 541 1074 Size: 541 533 SexAI A'CCwGG_T Cuts at: 0 553 1074 Size: 553 521 SfcI C'TryA_G Cuts at: 0 589 1074 Size: 589 485 SfiI GGCCn_nnn'nGGCC Cuts at: 0 728 1074 Size: 728 346 SfoI GGC'GCC Cuts at: 0 308 1074 Size: 308 766 SmaI CCC'GGG Cuts at: 0 549 735 1074 Size: 549 186 339 Fragments arranged by size: 549 339 186 SphI G_CATG'C Cuts at: 0 675 1074 Size: 675 399 StuI AGG'CCT Cuts at: 0 957 1074 Size: 957 117 StyI C'CwwG_G Cuts at: 0 386 764 934 1074 Size: 386 378 170 140 Fragments arranged by size: 386 378 170 140 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 229 1074 Size: 229 845 TatI w'GTAC_w Cuts at: 0 539 1074 Size: 539 535 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 148 180 1074 Size: 148 32 894 Fragments arranged by size: 894 148 32 XmaI C'CCGG_G Cuts at: 0 547 733 1074 Size: 547 186 341 Fragments arranged by size: 547 341 186 Enzymes that do cut: AcuI AfeI AhdI AlwNI ApaI ApaLI AscI AseI AvaI BanI BanII BbeI BcgI BglI Bme1580I BmgBI BmrI BpmI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BssHII BstEII BstXI Bsu36I BtgI BtsI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI SapI ScaI SexAI SfcI SfiI SfoI SmaI SphI StuI StyI TaqII TatI TstI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AflII AflIII AgeI AleI AlfI AloI ApoI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmtI BplI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BsmI BspEI BsrBI BsrGI BssSI BstAPI BstBI BstYI BstZ17I BtgZI ClaI CspCI DraI DraIII EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PfoI PmeI PshAI PsiI PspXI PsrI SacII SalI SanDI SbfI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI ZraI