(Circular) (Six-base) MAPSORT of: pFN21AB6104.seq Check: 4686 from: 1 to: 6547 pFN21AB6104 6547 bp With 182 enzymes: SgfI * January 7, 2013 17:02 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2667 4357 278 Size: 53 83 186 936 1131 1690 2468 Fragments arranged by size: 2468 1690 1131 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3890 1937 Size: 1953 4594 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2591 3498 4610 5994 6262 1632 Size: 959 907 1112 1384 268 1917 Fragments arranged by size: 1917 1384 1112 959 907 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 2203 3414 5446 6423 1203 Size: 1000 1211 2032 977 1327 Fragments arranged by size: 2032 1327 1211 1000 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 3570 5276 3570 Size: 1706 4841 AleI CACnn'nnGTG Cuts at: 1166 2313 1166 Size: 1147 5400 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6547 AlwNI CAG_nnn'CTG Cuts at: 2128 4290 5862 2128 Size: 2162 1572 2813 Fragments arranged by size: 2813 2162 1572 ApaLI G'TGCA_C Cuts at: 4604 5760 4604 Size: 1156 5391 ApoI r'AATT_y Cuts at: 1493 2017 2761 2837 3856 4082 4234 6343 1493 Size: 524 744 76 1019 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 744 524 226 152 76 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 3163 3189 3366 3505 3872 1870 Size: 73 1220 26 177 139 367 4545 Fragments arranged by size: 4545 1220 367 177 139 73 26 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2382 2895 3868 5323 618 Size: 325 217 627 595 513 973 1455 1842 Fragments arranged by size: 1842 1455 973 627 595 513 325 217 BanII G_rGCy'C Cuts at: 1391 3512 3866 1391 Size: 2121 354 4072 Fragments arranged by size: 4072 2121 354 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3213 3489 928 Size: 971 1314 276 3986 Fragments arranged by size: 3986 1314 971 276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2260 2294 4738 4772 1903 Size: 34 323 34 2444 34 3678 Fragments arranged by size: 3678 2444 323 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 2301 2346 1548 Size: 753 45 5749 Fragments arranged by size: 5749 753 45 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2462 5158 136 Size: 107 122 71 631 605 790 2696 1525 Fragments arranged by size: 2696 1525 790 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 3964 3964 Size: 6547 Bme1580I G_kGCm'C Cuts at: 1790 2900 4608 5764 1790 Size: 1110 1708 1156 2573 Fragments arranged by size: 2573 1708 1156 1110 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4278 5236 442 Size: 416 53 83 399 24 2861 958 1753 Fragments arranged by size: 2861 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2893 3011 3202 3395 5207 1239 Size: 1654 118 191 193 1812 2579 Fragments arranged by size: 2579 1812 1654 193 191 118 Bpu10I CC'TnA_GC Cuts at: 2150 2150 Size: 6547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3628 4672 5537 5835 3628 Size: 1044 865 298 4340 Fragments arranged by size: 4340 1044 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5210 882 Size: 617 3711 2219 Fragments arranged by size: 3711 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4338 493 Size: 711 3134 2702 Fragments arranged by size: 3134 2702 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2664 4354 4736 5371 Size: 53 83 186 936 1131 1690 382 635 Cuts at: 5371 275 Size: 1451 Fragments arranged by size: 1690 1451 1131 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2982 4980 5378 5652 5799 6210 2982 Size: 1998 398 274 147 411 3319 Fragments arranged by size: 3319 1998 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3200 3230 1178 Size: 30 165 30 1797 30 4495 Fragments arranged by size: 4495 1797 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 1186 Size: 1255 5292 BseYI C'CCAG_C Cuts at: 3747 5750 6246 3747 Size: 2003 496 4048 Fragments arranged by size: 4048 2003 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 5371 Fragments arranged by size: 5371 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3077 3997 4758 5786 1540 Size: 275 186 1076 920 761 1028 2301 Fragments arranged by size: 2301 1076 1028 920 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2421 3512 4608 4693 5764 2421 Size: 1091 1096 85 1071 3204 Fragments arranged by size: 3204 1096 1091 1071 85 BsmI GAATG_Cn' Cuts at: 4058 4151 4058 Size: 93 6454 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2421 2900 3512 3866 4608 4693 5764 Size: 399 631 479 612 354 742 85 1071 Cuts at: 5764 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 631 612 479 399 354 85 BspEI T'CCGG_A Cuts at: 2982 6210 2982 Size: 3228 3319 BspHI T'CATG_A Cuts at: 3820 4436 6166 3820 Size: 616 1730 4201 Fragments arranged by size: 4201 1730 616 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2154 3257 3647 3902 844 Size: 1310 1103 390 255 3489 Fragments arranged by size: 3489 1310 1103 390 255 BsrBI CCG'CTC Cuts at: 1394 1823 4434 1394 Size: 429 2611 3507 Fragments arranged by size: 3507 2611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3651 5042 5216 65 Size: 1183 2403 1391 174 1396 Fragments arranged by size: 2403 1396 1391 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3312 5191 5378 1700 Size: 110 1502 1879 187 2869 Fragments arranged by size: 2869 1879 1502 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6547 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 2058 2453 3738 794 Size: 1264 395 1285 3603 Fragments arranged by size: 3603 1285 1264 395 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstXI CCAn_nnnn'nTGG Cuts at: 2174 2253 2174 Size: 79 6468 BstYI r'GATC_y Cuts at: 1972 3370 3811 3877 4244 4629 4646 6098 6184 Size: 1398 441 66 367 385 17 1452 86 Cuts at: 6184 6196 6542 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1398 441 385 367 346 86 66 17 12 Bsu36I CC'TnA_GG Cuts at: 3286 3286 Size: 6547 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5995 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2700 487 Size: 683 56 1474 4334 Fragments arranged by size: 4334 1474 683 56 BtsI GCAGTG_nn' Cuts at: 2059 3100 3229 3672 3758 4063 4857 4877 2059 Size: 1041 129 443 86 305 794 20 3729 Fragments arranged by size: 3729 1041 794 443 305 129 86 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DraIII CAC_nnn'GTG Cuts at: 2895 2895 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3538 3700 3994 Size: 54 1425 50 275 18 1708 162 294 Cuts at: 3994 4883 6512 8 Size: 889 1629 43 Fragments arranged by size: 1708 1629 1425 889 294 275 162 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2766 2997 3315 3384 3517 3561 4477 1353 Size: 1413 231 318 69 133 44 916 3423 Fragments arranged by size: 3423 1413 916 318 231 133 69 44 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5134 5508 5654 387 Size: 1064 138 3545 374 146 1280 Fragments arranged by size: 3545 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3510 3510 Size: 6547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2591 2893 3011 3202 3395 3498 4610 Size: 393 959 302 118 191 193 103 1112 Cuts at: 4610 5207 5994 6262 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1112 959 787 597 393 302 268 193 191 118 103 EcoRI G'AATT_C Cuts at: 3856 4234 3856 Size: 378 6169 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2645 2645 Size: 6547 FspI TGC'GCA Cuts at: 3650 5053 3650 Size: 1403 5144 HaeII r_GCGC'y Cuts at: 1678 3121 5694 1678 Size: 1443 2573 2531 Fragments arranged by size: 2573 2531 1443 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2101 2133 3200 3232 3556 Size: 32 135 32 529 32 1067 32 324 Cuts at: 3556 3588 5188 5220 5262 5294 1373 Size: 32 1600 32 42 32 2626 Fragments arranged by size: 2626 1600 1067 529 324 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 2228 3530 748 Size: 1480 1302 3765 Fragments arranged by size: 3765 1480 1302 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2677 2791 3554 5661 5845 1305 Size: 1372 114 763 2107 184 2007 Fragments arranged by size: 2107 2007 1372 763 184 114 MscI TGG'CCA Cuts at: 10 64 1489 1832 3540 3702 10 Size: 54 1425 343 1708 162 2855 Fragments arranged by size: 2855 1708 1425 343 162 54 MslI CAynn'nnrTG Cuts at: 518 1166 2313 3443 4505 4864 5023 518 Size: 648 1147 1130 1062 359 159 2042 Fragments arranged by size: 2042 1147 1130 1062 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3064 3087 3963 4640 5788 6033 1606 Size: 1458 23 876 677 1148 245 2120 Fragments arranged by size: 2120 1458 1148 876 677 245 23 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5995 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 2207 2590 3418 3442 3905 5450 1132 Size: 97 978 383 828 24 463 1545 2229 Fragments arranged by size: 2229 1545 978 828 463 383 97 24 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6547 PciI A'CATG_T Cuts at: 2203 3414 5446 2203 Size: 1211 2032 3304 Fragments arranged by size: 3304 2032 1211 PflMI CCAn_nnn'nTGG Cuts at: 1108 2128 3709 1108 Size: 1020 1581 3946 Fragments arranged by size: 3946 1581 1020 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspXI vC'TCGA_Gb Cuts at: 3505 3505 Size: 6547 PstI C_TGCA'G Cuts at: 830 2463 2964 3254 3899 830 Size: 1633 501 290 645 3478 Fragments arranged by size: 3478 1633 645 501 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 3064 3064 Size: 6547 SacI G_AGCT'C Cuts at: 3512 3512 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SapI GCTCTTCn'nnn_ Cuts at: 3517 3517 Size: 6547 SbfI CC_TGCA'GG Cuts at: 2463 3899 2463 Size: 1436 5111 ScaI AGT'ACT Cuts at: 2324 3547 4795 6507 2324 Size: 1223 1248 1712 2364 Fragments arranged by size: 2364 1712 1248 1223 SfcI C'TryA_G Cuts at: 826 1045 2459 2680 2960 3250 3895 5030 5711 Size: 219 1414 221 280 290 645 1135 681 Cuts at: 5711 5902 826 Size: 191 1471 Fragments arranged by size: 1471 1414 1135 681 645 290 280 221 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3191 3368 3874 1872 Size: 1319 177 506 4545 Fragments arranged by size: 4545 1319 506 177 SmlI C'TyrA_G Cuts at: 820 1017 1943 3505 3607 4651 5552 5814 820 Size: 197 926 1562 102 1044 901 262 1553 Fragments arranged by size: 1562 1553 1044 926 901 262 197 102 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SspI AAT'ATT Cuts at: 5 52 2240 4471 5 Size: 47 2188 2231 2081 Fragments arranged by size: 2231 2188 2081 47 StuI AGG'CCT Cuts at: 1517 2432 3723 1517 Size: 915 1291 4341 Fragments arranged by size: 4341 1291 915 StyI C'CwwG_G Cuts at: 513 1065 3986 513 Size: 552 2921 3074 Fragments arranged by size: 3074 2921 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4574 4591 4744 4929 5374 325 Size: 1255 2994 17 153 185 445 1498 Fragments arranged by size: 2994 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 3545 4793 Size: 274 80 33 51 1443 345 1223 1248 Cuts at: 4793 6505 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1248 1223 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2805 2837 3137 3169 6507 6539 2805 Size: 32 300 32 3338 32 2813 Fragments arranged by size: 3338 2813 300 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6547 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 3505 1943 Size: 1562 4985 XmaI C'CCGG_G Cuts at: 1870 3189 3366 3872 1870 Size: 1319 177 506 4545 Fragments arranged by size: 4545 1319 506 177 XmnI GAAnn'nnTTC Cuts at: 3641 4676 3641 Size: 1035 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2665 4355 276 Size: 53 83 186 936 1131 1690 2468 Fragments arranged by size: 2468 1690 1131 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI