################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AvaI AviII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BpmI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsoBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraI DraII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I KasI KspI MluI MroI MslI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SbfI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI TthHB8I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 504 CCGC CCGC(-3/-1) AflIII 832 ACATGT A^C[GA][CT]GT AspI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 670 CCTAGG C^CTAGG BamHI 40 GGATCC G^GATCC BanII 787 GGGCTC G[GA]GC[CT]^C BcnI 546 CCCGG CC^[GC]GG BfaI 671 CTAG C^TAG BglI 600 GCCTCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 670 CCTAGG C^CTAGG BlpI 580 GCTGAGC GC^T[ACGT]AGC BmyI 787 GGGCTC G[AGT]GC[ACT]^C Bpu1102I 580 GCTGAGC GC^T[ACGT]AGC BsaAI 484 TACGTG [CT]AC^GT[GA] BsaBI 622 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 377 GAGACG CGTCTC(1/5) BsmI 161 GAATGC GAATGC(1/-1) Bsp1286I 787 GGGCTC G[AGT]GC[ACT]^C BspLU11I 832 ACATGT A^CATGT BsrBI 504 CCGCTC CCGCTC(-3/-3) BssSI 307 CTCGTG CACGAG(-5/-1) BstAPI 327 GCAGACACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 984 CCAGGTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 336 GCAAGC GC[ACGT]^[ACGT]GC CelII 580 GCTGAGC GC^T[ACGT]AGC DraIII 232 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 312 GAAGAG CTCTTC(1/4) EcoNI 978 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HapII 547 CCGG C^CGG HincII 193 GTTAAC GT[CT]^[GA]AC HindII 193 GTTAAC GT[CT]^[GA]AC HindIII 424 AAGCTT A^AGCTT HpaI 193 GTTAAC GTT^AAC HpaII 547 CCGG C^CGG Ksp632I 312 GAAGAG CTCTTC(1/4) MaeI 671 CTAG C^TAG MamI 622 GATGTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 547 CCGG C^CGG NciI 546 CCCGG CC^[GC]GG NspI 832 ACATGT [GA]CATG^[CT] PflFI 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 267 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 76 GACACGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 312 GAAGAGC GCTCTTC(1/4) SspI 245 AATATT AAT^ATT Tth111I 124 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 267 CCATGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### Acc65I 294 GGTACC G^GTACC 500 GGTACC AlwNI 328 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 725 CAGACCCTG Asp718I 294 GGTACC G^GTACC 500 GGTACC AvaII 460 GGACC G^G[AT]CC 813 GGACC BalI 92 TGGCCA TGG^CCA 607 TGGCCA BsmAI 377 GAGAC GTCTC(1/5) 803 GTCTC BsmFI 74 GGGAC GGGAC(10/14) 527 GGGAC BsrI 457 ACTGG ACTGG(1/-1) 991 ACTGG BstYI 40 GGATCC [GA]^GATC[CT] 660 GGATCT EaeI 92 TGGCCA [CT]^GGCC[GA] 607 TGGCCA EcoT14I 653 CCTTGG C^C[AT][AT]GG 670 CCTAGG HinfI 112 GAATC G^A[ACGT]TC 696 GAATC HphI 231 TCACC GGTGA(8/7) 852 TCACC KpnI 294 GGTACC GGTAC^C 500 GGTACC MaeII 380 ACGT A^CGT 485 ACGT MfeI 363 CAATTG C^AATTG 563 CAATTG MflI 40 GGATCC [GA]^GATC[CT] 660 GGATCT MluNI 92 TGGCCA TGG^CCA 607 TGGCCA MscI 92 TGGCCA TGG^CCA 607 TGGCCA MspA1I 631 CAGCTG C[AC]G^C[GT]G 841 CAGCTG MunI 363 CAATTG C^AATTG 563 CAATTG PvuII 631 CAGCTG CAG^CTG 841 CAGCTG ScaI 701 AGTACT AGT^ACT 1028 AGTACT SexAI 233 ACCTGGT A^CC[AT]GGT 493 ACCAGGT SmlI 166 CTCAAG C^T[CT][GA]AG 175 CTCAAG StyI 653 CCTTGG C^C[AT][AT]GG 670 CCTAGG TfiI 112 GAATC G^A[AT]TC 696 GAATC XhoII 40 GGATCC [GA]^GATC[CT] 660 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 43 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BanI BbsI BbvI BpuAI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII ItaI MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI Tru9I TseI Tsp45I Tsp509I TspRI