################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI MaeII MaeIII MamI MfeI MluI MroI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NlaIII NotI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 320 GGTACC G^GTACC AfaI 321 GTAC GT^AC AlwNI 283 CAGACGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 320 GGTACC G^GTACC AvaII 643 GGTCC G^G[AT]CC AviII 32 TGCGCA TGC^GCA BalI 2 TGGCCA TGG^CCA BanII 231 GGGCTC G[GA]GC[CT]^C BbeI 595 GGCGCC GGCGC^C BglII 386 AGATCT A^GATCT BmyI 231 GGGCTC G[AGT]GC[ACT]^C BsgI 475 CTGCAC GTGCAG(16/14) BsiEI 205 CGGCCG CG[GA][CT]^CG BsmBI 224 CGTCTC CGTCTC(1/5) BsmFI 644 GTCCC GGGAC(10/14) Bsp1286I 231 GGGCTC G[AGT]GC[ACT]^C BsrBI 298 GAGCGG CCGCTC(-3/-3) BsrFI 513 GCCGGT [GA]^CCGG[CT] BsrI 713 ACTGG ACTGG(1/-1) BssHII 420 GCGCGC G^CGCGC BstAPI 66 GCAACACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 386 AGATCT [GA]^GATC[CT] Cfr10I 513 GCCGGT [GA]^CCGG[CT] DpnII 387 GATC ^GATC DsaI 575 CCGCGG C^C[GA][CT]GG EagI 205 CGGCCG C^GGCCG EarI 664 CTCTTC CTCTTC(1/4) EclXI 205 CGGCCG C^GGCCG Eco52I 205 CGGCCG C^GGCCG EcoT14I 275 CCAAGG C^C[AT][AT]GG FspI 32 TGCGCA TGC^GCA HphI 26 TCACC GGTGA(8/7) KasI 595 GGCGCC G^GCGCC KpnI 320 GGTACC GGTAC^C Ksp632I 664 CTCTTC CTCTTC(1/4) KspI 575 CCGCGG CCGC^GG MboI 387 GATC ^GATC MflI 386 AGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA NarI 595 GGCGCC GG^CGCC NdeII 387 GATC ^GATC NruI 525 TCGCGA TCG^CGA PvuII 346 CAGCTG CAG^CTG RsaI 321 GTAC GT^AC SacII 575 CCGCGG CCGC^GG Sau3AI 387 GATC ^GATC SfoI 595 GGCGCC GGC^GCC StyI 275 CCAAGG C^C[AT][AT]GG XbaI 42 TCTAGA T^CTAGA XhoII 386 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AvaI 52 CCCGAG C^[CT]CG[GA]G 334 CCCGAG BfaI 43 CTAG C^TAG 215 CTAG BsaI 178 GAGACC GGTCTC(1/5) 702 GGTCTC BsoBI 52 CCCGAG C^[CT]CG[GA]G 334 CCCGAG BspMI 71 ACCTGC ACCTGC(4/8) 378 GCAGGT BstF5I 464 CATCC GGATG(2/0) 689 CATCC DdeI 19 CTGAG C^T[ACGT]AG 684 CTTAG FokI 464 CATCC GGATG(9/13) 689 CATCC MaeI 43 CTAG C^TAG 215 CTAG MboII 252 GAAGA GAAGA(8/7) 665 TCTTC PleI 193 GACTC GAGTC(4/5) 445 GAGTC SfaNI 463 GCATC GCATC(5/9) 688 GCATC TfiI 133 GATTC G^A[AT]TC 399 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AccII AciI AcyI AluI BanI BbvI BcnI BpmI BsaHI BsaJI BsiYI BslI BsmAI BssKI BstNI BstUI Cac8I CfoI Cfr13I EaeI EcoRII FauI Fnu4HI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI TseI