################################## # NO CUT ENZYMES ( 131 enzymes) # ################################## AatII Acc65I AccI AclI AcyI AflII AflIII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaI BsiEI BsiWI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI TaqI Tsp45I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 685 CGCG CG^CG Alw44I 257 GTGCAC G^TGCAC AlwI 609 GGATC GGATC(4/5) AocI 740 CCTCAGG CC^T[ACGT]AGG ApaI 1017 GGGCCC GGGCC^C ApaLI 257 GTGCAC G^TGCAC AspI 991 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 854 CCCGAG C^[CT]CG[GA]G BbsI 240 GAAGAC GAAGAC(2/6) BfaI 838 CTAG C^TAG BpuAI 240 GAAGAC GAAGAC(2/6) BsmBI 357 CGTCTC CGTCTC(1/5) BsmI 900 GAATGC GAATGC(1/-1) BsoBI 854 CCCGAG C^[CT]CG[GA]G BspMI 765 GCAGGT ACCTGC(4/8) BstUI 685 CGCG CG^CG BstXI 229 CCAGCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 740 CCTCAGG CC^T[ACGT]AGG DpnII 610 GATC ^GATC DsaI 1065 CCATGG C^C[GA][CT]GG Eco81I 740 CCTCAGG CC^T[ACGT]AGG EcoT14I 1065 CCATGG C^C[AT][AT]GG FauI 930 CCCGC CCCGC(4/6) HphI 665 TCACC GGTGA(8/7) MaeI 838 CTAG C^TAG MboI 610 GATC ^GATC MslI 834 CATCCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 230 CAGCTG C[AC]G^C[GT]G MvnI 685 CGCG CG^CG NcoI 1065 CCATGG C^CATGG NdeII 610 GATC ^GATC NspI 129 ACATGC [GA]CATG^[CT] PflFI 991 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 890 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 616 AGGTCCC [GA]G^G[AT]CC[CT] PstI 984 CTGCAG CTGCA^G PvuII 230 CAGCTG CAG^CTG SacI 961 GAGCTC GAGCT^C SapI 963 GCTCTTC GCTCTTC(1/4) Sau3AI 610 GATC ^GATC SfcI 984 CTGCAG C^T[GA][CT]AG SmlI 823 CTTGAG C^T[CT][GA]AG SspI 189 AATATT AAT^ATT StyI 1065 CCATGG C^C[AT][AT]GG Tth111I 991 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 890 CCATCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccIII 35 TCCGGA T^CCGGA 113 TCCGGA AcsI 277 GAATTT [GA]^AATT[CT] 1047 GAATTT AfaI 385 GTAC GT^AC 1002 GTAC ApoI 277 GAATTT [GA]^AATT[CT] 1047 GAATTT BalI 363 TGGCCA TGG^CCA 842 TGGCCA BcnI 76 CCCGG CC^[GC]GG 150 CCGGG BsaWI 35 TCCGGA [AT]^CCGG[AT] 113 TCCGGA BseAI 35 TCCGGA T^CCGGA 113 TCCGGA BseRI 496 GAGGAG GAGGAG(10/8) 499 GAGGAG BsmAI 989 GAGAC GTCTC(1/5) 358 GTCTC BspEI 35 TCCGGA T^CCGGA 113 TCCGGA EaeI 363 TGGCCA [CT]^GGCC[GA] 842 TGGCCA EcoNI 416 CCTCTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 919 CCTGGGCCAGG HaeII 806 AGCGCC [GA]GCGC^[CT] 872 AGCGCC MaeIII 577 GTTAC ^GT[ACGT]AC 1039 GTAAC MluNI 363 TGGCCA TGG^CCA 842 TGGCCA MroI 35 TCCGGA T^CCGGA 113 TCCGGA MscI 363 TGGCCA TGG^CCA 842 TGGCCA MseI 194 TTAA T^TAA 281 TTAA NciI 76 CCCGG CC^[GC]GG 150 CCGGG RsaI 385 GTAC GT^AC 1002 GTAC Tru9I 194 TTAA T^TAA 281 TTAA Tsp509I 278 AATT ^AATT 1048 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwNI AspHI AvaII BanII BbvI BlpI BmyI BpmI Bpu1102I BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstAPI BstF5I BstNI Cac8I CelII CfoI Cfr13I DdeI DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI Ksp632I MboII MnlI MspI MvaI MwoI NlaIII NlaIV PleI Sau96I ScrFI SfaNI StuI TfiI TseI TspRI XcmI