(Circular) (Six-base) MAPSORT of: pFN21AE1872.seq Check: 9134 from: 1 to: 5884 pFN21AE1872 5884 bp With 182 enzymes: SgfI * March 28, 2012 13:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2761 2761 Size: 5884 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3694 278 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3227 1937 Size: 1290 4594 Acc65I G'GTAC_C Cuts at: 3205 3205 Size: 5884 AclI AA'CG_TT Cuts at: 1563 4011 4384 1563 Size: 2448 373 3063 Fragments arranged by size: 3063 2448 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2956 3947 5331 5599 1632 Size: 1324 991 1384 268 1917 Fragments arranged by size: 1917 1384 1324 991 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5687 AflIII A'CryG_T Cuts at: 1203 4783 5760 1203 Size: 3580 977 1327 Fragments arranged by size: 3580 1327 977 AgeI A'CCGG_T Cuts at: 4715 4715 Size: 5884 AhdI GACnn_n'nnGTC Cuts at: 4613 4613 Size: 5884 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3141 1184 Size: 1957 3927 AlwNI CAG_nnn'CTG Cuts at: 2347 2753 2776 2815 3019 3627 5199 2347 Size: 406 23 39 204 608 1572 3032 Fragments arranged by size: 3032 1572 608 406 204 39 23 ApaI G_GGCC'C Cuts at: 3026 3026 Size: 5884 ApaLI G'TGCA_C Cuts at: 2262 3941 5097 2262 Size: 1679 1156 3049 Fragments arranged by size: 3049 1679 1156 ApoI r'AATT_y Cuts at: 1493 2282 3052 3193 3419 3571 5680 1493 Size: 789 770 141 226 152 2109 1697 Fragments arranged by size: 2109 1697 789 770 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 4438 160 Size: 872 3406 1606 Fragments arranged by size: 3406 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 AvaI C'yCGr_G Cuts at: 1870 1943 2859 3209 1870 Size: 73 916 350 4545 Fragments arranged by size: 4545 916 350 73 BamHI G'GATC_C Cuts at: 3214 3581 3214 Size: 367 5517 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3205 4660 618 Size: 325 217 627 1418 1455 1842 Fragments arranged by size: 1842 1455 1418 627 325 217 BanII G_rGCy'C Cuts at: 1391 2328 2745 2805 2970 3026 3203 1391 Size: 937 417 60 165 56 177 4072 Fragments arranged by size: 4072 937 417 177 165 60 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2252 928 Size: 971 353 4560 Fragments arranged by size: 4560 971 353 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4075 4109 1903 Size: 34 2138 34 3678 Fragments arranged by size: 3678 2138 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3776 4986 3776 Size: 1210 4674 BclI T'GATC_A Cuts at: 1548 1548 Size: 5884 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4495 136 Size: 107 122 71 631 605 2823 1525 Fragments arranged by size: 2823 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5879 5879 Size: 5884 BlpI GC'TnA_GC Cuts at: 2322 2739 2819 3301 2322 Size: 417 80 482 4905 Fragments arranged by size: 4905 482 417 80 Bme1580I G_kGCm'C Cuts at: 1790 2023 2266 3026 3945 5101 1790 Size: 233 243 760 919 1156 2573 Fragments arranged by size: 2573 1156 919 760 243 233 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5884 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2056 3615 4573 Size: 416 53 83 399 24 639 1559 958 Cuts at: 4573 442 Size: 1753 Fragments arranged by size: 1753 1559 958 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2465 2628 2648 2819 3061 3135 3161 4544 Size: 1226 163 20 171 242 74 26 1383 Cuts at: 4544 1239 Size: 2579 Fragments arranged by size: 2579 1383 1226 242 171 163 74 26 20 Bpu10I CC'TnA_GC Cuts at: 2568 2568 Size: 5884 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2849 4009 4874 5172 2849 Size: 1160 865 298 3561 Fragments arranged by size: 3561 1160 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4547 882 Size: 617 3048 2219 Fragments arranged by size: 3048 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3675 493 Size: 711 2471 2702 Fragments arranged by size: 2702 2471 711 BsaBI GATnn'nnATC Cuts at: 5878 5878 Size: 5884 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3691 4073 4708 275 Size: 53 83 186 936 2158 382 635 1451 Fragments arranged by size: 2158 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2040 2118 4317 4715 4989 5136 5547 2040 Size: 78 2199 398 274 147 411 2377 Fragments arranged by size: 2377 2199 411 398 274 147 78 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5659 Fragments arranged by size: 5659 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2516 2519 1186 Size: 1330 3 4551 Fragments arranged by size: 4551 1330 3 BseYI C'CCAG_C Cuts at: 2091 2948 5087 5583 2091 Size: 857 2139 496 2392 Fragments arranged by size: 2392 2139 857 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 2443 2894 1163 Size: 573 275 432 451 4153 Fragments arranged by size: 4153 573 451 432 275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3334 4095 5123 1540 Size: 275 186 1333 761 1028 2301 Fragments arranged by size: 2301 1333 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2266 2481 2970 3945 4030 5101 2266 Size: 215 489 975 85 1071 3049 Fragments arranged by size: 3049 1071 975 489 215 85 BsmI GAATG_Cn' Cuts at: 2911 3395 3488 2911 Size: 484 93 5307 Fragments arranged by size: 5307 484 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2368 2368 Size: 5884 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2023 2266 2328 2481 2745 2805 2970 Size: 399 233 243 62 153 264 60 165 Cuts at: 2970 3026 3203 3945 4030 5101 1391 Size: 56 177 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 264 243 233 177 165 153 85 62 60 56 BspEI T'CCGG_A Cuts at: 2040 2118 5547 2040 Size: 78 3429 2377 Fragments arranged by size: 3429 2377 78 BspHI T'CATG_A Cuts at: 3773 5503 3773 Size: 1730 4154 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2761 3239 844 Size: 1917 478 3489 Fragments arranged by size: 3489 1917 478 BsrBI CCG'CTC Cuts at: 1394 1823 3771 1394 Size: 429 1948 3507 Fragments arranged by size: 3507 1948 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4379 4553 65 Size: 1183 3131 174 1396 Fragments arranged by size: 3131 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4528 4715 1700 Size: 110 2718 187 2869 Fragments arranged by size: 2869 2718 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5884 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5884 BssSI C'ACGA_G Cuts at: 3944 4956 3944 Size: 1012 4872 BstAPI GCAn_nnn'nTGC Cuts at: 794 2815 2944 2958 794 Size: 2021 129 14 3720 Fragments arranged by size: 3720 2021 129 14 BstBI TT'CG_AA Cuts at: 4688 4688 Size: 5884 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5884 BstXI CCAn_nnnn'nTGG Cuts at: 2241 2241 Size: 5884 BstYI r'GATC_y Cuts at: 1972 3214 3581 3966 3983 5435 5521 5533 5879 Size: 1242 367 385 17 1452 86 12 346 Cuts at: 5879 1972 Size: 1977 Fragments arranged by size: 1977 1452 1242 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2746 2746 Size: 5884 BtgI C'CryG_G Cuts at: 513 1065 2004 3070 513 Size: 552 939 1066 3327 Fragments arranged by size: 3327 1066 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5145 Fragments arranged by size: 5145 683 56 BtsI GCAGTG_nn' Cuts at: 2660 3400 4194 4214 2660 Size: 740 794 20 4330 Fragments arranged by size: 4330 794 740 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5884 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5849 DraI TTT'AAA Cuts at: 3188 3188 Size: 5884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4891 809 Size: 507 3575 1802 Fragments arranged by size: 3575 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2368 2847 3331 Size: 54 1425 50 275 18 538 479 484 Cuts at: 3331 4220 5849 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 538 484 479 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3331 1537 Size: 275 1519 4090 Fragments arranged by size: 4090 1519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2433 2873 2975 3057 3814 1353 Size: 1080 440 102 82 757 3423 Fragments arranged by size: 3423 1080 757 440 102 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4471 4845 4991 387 Size: 1064 138 2882 374 146 1280 Fragments arranged by size: 2882 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2968 2968 Size: 5884 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2465 2628 2648 2819 2956 3061 3135 Size: 393 833 163 20 171 137 105 74 Cuts at: 3135 3161 3947 4544 5331 5599 1239 Size: 26 786 597 787 268 1524 Fragments arranged by size: 1524 833 787 786 597 393 268 171 163 137 105 74 26 20 EcoNI CCTnn'n_nnAGG Cuts at: 2425 2928 2425 Size: 503 5381 EcoO109I rG'GnC_Cy Cuts at: 2599 2622 3016 3023 2599 Size: 23 394 7 5460 Fragments arranged by size: 5460 394 23 7 EcoRI G'AATT_C Cuts at: 3193 3571 3193 Size: 378 5506 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2167 2167 Size: 5884 FspI TGC'GCA Cuts at: 4390 4390 Size: 5884 HaeII r_GCGC'y Cuts at: 1678 2815 2881 5031 1678 Size: 1137 66 2150 2531 Fragments arranged by size: 2531 2150 1137 66 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2606 2638 2985 3017 4525 Size: 32 135 32 1034 32 347 32 1508 Cuts at: 4525 4557 4599 4631 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1508 1034 347 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3228 3474 669 Size: 988 281 1290 246 3079 Fragments arranged by size: 3079 1290 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5884 HpaI GTT'AAC Cuts at: 3474 3474 Size: 5884 KpnI G_GTAC'C Cuts at: 3209 3209 Size: 5884 MfeI C'AATT_G Cuts at: 3483 3483 Size: 5884 MluI A'CGCG_T Cuts at: 5760 5760 Size: 5884 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4998 5182 1305 Size: 3693 184 2007 Fragments arranged by size: 3693 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2370 2849 10 Size: 54 1425 343 538 479 3045 Fragments arranged by size: 3045 1425 538 479 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2843 3842 4201 4360 518 Size: 648 1677 999 359 159 2042 Fragments arranged by size: 2042 1677 999 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2237 3300 3977 5125 5370 1606 Size: 631 1063 677 1148 245 2120 Fragments arranged by size: 2120 1148 1063 677 631 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3070 513 Size: 552 939 1066 3327 Fragments arranged by size: 3327 1066 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5884 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5884 NotI GC'GGCC_GC Cuts at: 3331 3331 Size: 5884 NspI r_CATG'y Cuts at: 1132 1229 2138 3242 4787 1132 Size: 97 909 1104 1545 2229 Fragments arranged by size: 2229 1545 1104 909 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5884 PciI A'CATG_T Cuts at: 4783 4783 Size: 5884 PflMI CCAn_nnn'nTGG Cuts at: 1108 2901 1108 Size: 1793 4091 PfoI T'CCnGG_A Cuts at: 2080 2154 2481 2900 2080 Size: 74 327 419 5064 Fragments arranged by size: 5064 419 327 74 PmeI GTTT'AAAC Cuts at: 3188 3188 Size: 5884 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3953 3985 5466 5498 3953 Size: 32 1481 32 4339 Fragments arranged by size: 4339 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2622 2622 Size: 5884 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5884 PsiI TTA'TAA Cuts at: 3454 3454 Size: 5884 PspOMI G'GGCC_C Cuts at: 3022 3022 Size: 5884 PstI C_TGCA'G Cuts at: 830 2993 3236 830 Size: 2163 243 3478 Fragments arranged by size: 3478 2163 243 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5884 PvuII CAG'CTG Cuts at: 2237 2237 Size: 5884 SacI G_AGCT'C Cuts at: 2970 2970 Size: 5884 SalI G'TCGA_C Cuts at: 1936 3226 1936 Size: 1290 4594 SapI GCTCTTCn'nnn_ Cuts at: 2975 2975 Size: 5884 SbfI CC_TGCA'GG Cuts at: 3236 3236 Size: 5884 ScaI AGT'ACT Cuts at: 4132 5844 4132 Size: 1712 4172 SfcI C'TryA_G Cuts at: 826 1045 2989 3232 4367 5048 5239 826 Size: 219 1944 243 1135 681 191 1471 Fragments arranged by size: 1944 1471 1135 681 243 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5884 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5884 SmaI CCC'GGG Cuts at: 1872 3211 1872 Size: 1339 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 3988 4889 5151 820 Size: 197 926 885 1160 901 262 1553 Fragments arranged by size: 1553 1160 926 901 885 262 197 SnaBI TAC'GTA Cuts at: 493 3675 493 Size: 3182 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5884 SphI G_CATG'C Cuts at: 1132 3242 1132 Size: 2110 3774 SspI AAT'ATT Cuts at: 5 52 2196 3808 5 Size: 47 2144 1612 2081 Fragments arranged by size: 2144 2081 1612 47 StuI AGG'CCT Cuts at: 1517 2186 2865 3122 1517 Size: 669 679 257 4279 Fragments arranged by size: 4279 679 669 257 StyI C'CwwG_G Cuts at: 513 1065 2004 3070 3323 513 Size: 552 939 1066 253 3074 Fragments arranged by size: 3074 1066 939 552 253 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2038 3911 3928 4081 4266 4711 325 Size: 1255 458 1873 17 153 185 445 1498 Fragments arranged by size: 1873 1498 1255 458 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4130 5842 96 Size: 274 80 33 51 1443 2153 1712 138 Fragments arranged by size: 2153 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2215 2247 2452 2484 3021 3053 5844 5876 2215 Size: 32 205 32 537 32 2791 32 2223 Fragments arranged by size: 2791 2223 537 205 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2999 1316 Size: 1683 4201 XbaI T'CTAG_A Cuts at: 3220 3220 Size: 5884 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2537 2921 3175 2537 Size: 384 254 5246 Fragments arranged by size: 5246 384 254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5884 XmaI C'CCGG_G Cuts at: 1870 3209 1870 Size: 1339 4545 XmnI GAAnn'nnTTC Cuts at: 4013 4013 Size: 5884 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3692 276 Size: 53 83 186 936 2158 2468 Fragments arranged by size: 2468 2158 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI